FastQCFastQC Report
Sat 18 Jun 2016
SRR3553295_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553295_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences566089
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA149602.642693993347336No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34140.6030853805673666No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27070.4781933582881844No Hit
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT22710.40117366703822194Illumina Single End Adapter 1 (95% over 21bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14900.26320949532670657No Hit
GACGAGGGGGAGAAGAAGAAGAAGAAGAGGAAGAAGAAGAAGAAGAAGAAG10690.18883956409681163No Hit
GCAGAGGGACATATTGTTGATTTATTATTTTTAAGAGTACAGACTAACTGA5850.10334064078263312No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG207.030248E-445.00000434
GGTACGA406.8048394E-945.0000049
GATAATC207.030248E-445.0000049
CGAGTAT207.030248E-445.00000434
ACGTTTA207.030248E-445.00000438
CTATCGG207.030248E-445.0000042
TAGACCC207.030248E-445.00000432
GACCGTA207.030248E-445.00000414
TAGCGAT207.030248E-445.00000445
ATTACCG207.030248E-445.0000041
TAAACCG406.8048394E-945.0000041
CCGTGAT207.030248E-445.00000421
TCCTCGA406.8048394E-945.00000422
TAGCCCG207.030248E-445.0000041
ATCGTAG207.030248E-445.0000041
CTGCGCT207.030248E-445.00000415
TGCGCAG406.8048394E-945.0000041
CATAGCG207.030248E-445.0000041
CGTAGCA207.030248E-445.00000417
CGCTAAT207.030248E-445.00000410