Basic Statistics
Measure | Value |
---|---|
Filename | SRR3553293_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 615359 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 46 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 16851 | 2.738401485961853 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 3758 | 0.6107004204049994 | No Hit |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 3147 | 0.5114087873907751 | No Hit |
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT | 1994 | 0.32403848810206726 | TruSeq Adapter, Index 22 (95% over 23bp) |
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1723 | 0.2799991549648254 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGT | 1356 | 0.22035917245055323 | No Hit |
AATGATACGGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCC | 1066 | 0.17323221079077417 | No Hit |
AATCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT | 894 | 0.14528104732359484 | No Hit |
CTGTCTGGGGCTCCTGTTTTTTAGCTGCTGTTCCTTGTCAGCTCTGACCAT | 810 | 0.13163047911869333 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTACAG | 85 | 0.0 | 45.000004 | 1 |
TAGAGTC | 20 | 7.030729E-4 | 45.0 | 38 |
AAACTCG | 20 | 7.030729E-4 | 45.0 | 1 |
AGCCGTC | 25 | 3.8886858E-5 | 45.0 | 13 |
GTCGCTA | 20 | 7.030729E-4 | 45.0 | 33 |
TCGCATC | 25 | 3.8886858E-5 | 45.0 | 15 |
TACCCTA | 35 | 1.2107012E-7 | 45.0 | 19 |
GGTACGT | 20 | 7.030729E-4 | 45.0 | 8 |
ACCGGTA | 25 | 3.8886858E-5 | 45.0 | 32 |
AGATCAC | 25 | 3.8886858E-5 | 45.0 | 37 |
TCCGCGT | 25 | 3.8886858E-5 | 45.0 | 11 |
CTCGTCG | 20 | 7.030729E-4 | 45.0 | 30 |
AGTTTCG | 20 | 7.030729E-4 | 45.0 | 45 |
GATCGAG | 20 | 7.030729E-4 | 45.0 | 1 |
CGAGTAT | 25 | 3.8886858E-5 | 45.0 | 13 |
CAACGGC | 20 | 7.030729E-4 | 45.0 | 16 |
TTTACCG | 20 | 7.030729E-4 | 45.0 | 1 |
CCTAGTA | 20 | 7.030729E-4 | 45.0 | 12 |
AGATACG | 20 | 7.030729E-4 | 45.0 | 1 |
ACGCATG | 25 | 3.8886858E-5 | 45.0 | 1 |