FastQCFastQC Report
Sat 18 Jun 2016
SRR3553293_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553293_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences615359
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA168512.738401485961853No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37580.6107004204049994No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31470.5114087873907751No Hit
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT19940.32403848810206726TruSeq Adapter, Index 22 (95% over 23bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17230.2799991549648254No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGT13560.22035917245055323No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCC10660.17323221079077417No Hit
AATCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT8940.14528104732359484No Hit
CTGTCTGGGGCTCCTGTTTTTTAGCTGCTGTTCCTTGTCAGCTCTGACCAT8100.13163047911869333No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACAG850.045.0000041
TAGAGTC207.030729E-445.038
AAACTCG207.030729E-445.01
AGCCGTC253.8886858E-545.013
GTCGCTA207.030729E-445.033
TCGCATC253.8886858E-545.015
TACCCTA351.2107012E-745.019
GGTACGT207.030729E-445.08
ACCGGTA253.8886858E-545.032
AGATCAC253.8886858E-545.037
TCCGCGT253.8886858E-545.011
CTCGTCG207.030729E-445.030
AGTTTCG207.030729E-445.045
GATCGAG207.030729E-445.01
CGAGTAT253.8886858E-545.013
CAACGGC207.030729E-445.016
TTTACCG207.030729E-445.01
CCTAGTA207.030729E-445.012
AGATACG207.030729E-445.01
ACGCATG253.8886858E-545.01