##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553293_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	615359
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.672366862270643	31.0	31.0	34.0	30.0	34.0
2	31.868354895272514	33.0	31.0	34.0	30.0	34.0
3	32.02787966049087	33.0	31.0	34.0	30.0	34.0
4	35.74836477568379	37.0	35.0	37.0	35.0	37.0
5	35.52903102091624	37.0	35.0	37.0	33.0	37.0
6	32.729565993184465	37.0	35.0	37.0	26.0	37.0
7	34.22832037883577	37.0	35.0	37.0	28.0	37.0
8	35.327480381370876	37.0	35.0	37.0	32.0	37.0
9	37.19694032264093	39.0	37.0	39.0	34.0	39.0
10	36.77325431171072	39.0	37.0	39.0	32.0	39.0
11	36.7746599952223	39.0	37.0	39.0	32.0	39.0
12	36.6999393849769	39.0	35.0	39.0	32.0	39.0
13	36.68909693365986	39.0	35.0	39.0	32.0	39.0
14	37.810478111151376	40.0	37.0	41.0	33.0	41.0
15	37.87071936869372	40.0	37.0	41.0	33.0	41.0
16	37.771643869676076	40.0	37.0	41.0	33.0	41.0
17	37.73766533031937	39.0	37.0	41.0	33.0	41.0
18	37.57957387476254	39.0	36.0	41.0	33.0	41.0
19	37.53717748501281	39.0	36.0	41.0	32.0	41.0
20	37.43074367970567	39.0	35.0	41.0	32.0	41.0
21	37.36555409118905	39.0	35.0	41.0	32.0	41.0
22	37.32385485545836	39.0	35.0	41.0	32.0	41.0
23	37.15815320812729	39.0	35.0	40.0	32.0	41.0
24	37.023384723389114	39.0	35.0	40.0	31.0	41.0
25	37.04060069000372	39.0	35.0	40.0	31.0	41.0
26	36.92358931940542	39.0	35.0	40.0	31.0	41.0
27	36.8310612179232	39.0	35.0	40.0	31.0	41.0
28	36.75832806540572	39.0	35.0	40.0	31.0	41.0
29	36.60299597470745	39.0	35.0	40.0	31.0	41.0
30	36.59516314866606	39.0	35.0	40.0	31.0	41.0
31	36.20150026244843	38.0	35.0	40.0	30.0	41.0
32	36.26040408932022	38.0	35.0	40.0	30.0	41.0
33	36.23744513365369	38.0	35.0	40.0	30.0	41.0
34	36.216213624892134	38.0	35.0	40.0	30.0	41.0
35	36.11791490820806	38.0	35.0	40.0	30.0	41.0
36	35.990436476918354	38.0	35.0	40.0	30.0	41.0
37	35.970422143821736	38.0	35.0	40.0	30.0	41.0
38	35.80109009537522	38.0	35.0	40.0	29.0	41.0
39	35.71964495522126	38.0	35.0	40.0	29.0	41.0
40	35.564155232961575	38.0	34.0	40.0	28.0	41.0
41	35.52429069860033	38.0	34.0	40.0	28.0	41.0
42	35.4473096192629	38.0	34.0	40.0	28.0	41.0
43	35.348068363345625	38.0	34.0	40.0	27.0	41.0
44	35.182820109887075	38.0	34.0	40.0	27.0	41.0
45	34.95688208021659	37.0	34.0	40.0	26.0	41.0
46	34.6535112024038	37.0	33.0	40.0	25.0	41.0
47	34.58526648671751	37.0	33.0	40.0	25.0	41.0
48	34.560207943655655	37.0	33.0	40.0	25.0	41.0
49	34.470237698644205	36.0	33.0	40.0	25.0	41.0
50	34.33470055691068	36.0	33.0	40.0	24.0	41.0
51	33.22917191428093	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	6.0
10	19.0
11	20.0
12	34.0
13	41.0
14	56.0
15	72.0
16	92.0
17	193.0
18	393.0
19	741.0
20	1174.0
21	1900.0
22	2454.0
23	3121.0
24	3852.0
25	4576.0
26	5372.0
27	6736.0
28	8262.0
29	10330.0
30	13417.0
31	17562.0
32	22897.0
33	31471.0
34	46577.0
35	62860.0
36	66664.0
37	87805.0
38	112617.0
39	104024.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.537183985283388	31.56628894677741	19.828912878498567	27.067614189440636
2	24.369676887800455	33.38538966684489	18.960151716315192	23.284781729039473
3	20.584894346227163	34.27381414751389	20.08209841734662	25.059193088912323
4	20.432300494508084	35.30378201992658	18.08635284443715	26.177564641128185
5	17.777427485419082	37.14010845701453	16.094832447400623	28.98763161016577
6	17.89621993015459	44.70772345898898	17.729650496701925	19.6664061141545
7	81.42758942340976	10.705458114694025	4.562702422488337	3.3042500394078904
8	82.79654640624416	9.138242879359854	3.853360396126489	4.211850318269498
9	76.71928094006914	12.796920171802151	6.240584764340816	4.243214123787903
10	35.343596177190875	35.90456952770659	12.688365653220316	16.063468641882217
11	26.436600423492628	32.79679016639068	22.54570096480266	18.220908445314034
12	24.616362156074747	29.31248263208956	27.07736459530128	18.993790616534415
13	23.412024525520874	29.738575368199697	24.935362934482146	21.914037171797276
14	18.961451770429942	32.313820062760115	27.58666079475558	21.138067372054362
15	19.998244926945084	33.28918566235319	25.8239499219155	20.88861948878622
16	21.147817777915005	30.92081207880278	26.98554827344688	20.94582186983533
17	21.43740483197613	30.5067448432541	27.081102250881194	20.974748073888573
18	22.040954954749992	30.337412794807584	25.299540593377202	22.322091657065226
19	22.24506345076614	31.9103157668938	25.29726549867638	20.54735528366368
20	23.06052239424466	31.681993762990384	24.713866214681186	20.54361762808377
21	22.879164845236684	30.57223506928476	25.985969165966534	20.562630919512024
22	21.88348590010059	30.31969955749408	24.78796929922208	23.00884524318325
23	21.193644685460033	32.86650556829428	23.748088514184403	22.191761232061285
24	19.745871921918752	31.925591402742135	26.755926215428715	21.572610459910393
25	21.272947986459936	31.74943407019317	24.266972612735003	22.710645330611886
26	22.56130161417969	31.823699661498413	24.3163746690956	21.2986240552263
27	19.403145155917116	31.900727867797496	26.16878927585361	22.52733770043178
28	20.049434557713464	32.22346630178482	27.506544959933958	20.220554180567767
29	21.53458387705388	33.161617852343106	24.31474960145216	20.989048669150854
30	22.037542312698765	31.14848405564882	25.055455433332412	21.758518198320004
31	23.530329449963354	32.059821990090335	22.72982112880449	21.68002743114182
32	21.61599976599026	32.842617073935706	24.23690886133135	21.304474298742683
33	21.980664945178344	30.793731789085722	25.205124163293295	22.02047910244264
34	21.597799008383724	30.953800951964627	24.745392526963936	22.703007512687716
35	21.22110832863418	31.68459387121989	25.77259778438277	21.321700015763156
36	22.501986645194105	33.712515783469485	24.802269894484358	18.98322767685205
37	23.193777941006793	31.499661173396344	22.20589932055922	23.100661565037644
38	21.812470444082237	31.914540942766745	25.23957559733424	21.033413015816784
39	22.636217232542304	29.275106076290424	24.2588472745178	23.829829416649467
40	22.258063991913662	29.29363184742565	25.490486041481475	22.957818119179212
41	19.515437330078864	30.83338343958567	26.154976200884363	23.496203029451102
42	22.110995370182284	31.23331258663642	23.22562926681823	23.430062776363066
43	21.271485425580842	30.707765710747708	25.37591877261891	22.64483009105254
44	21.699853256391798	28.82057465642007	25.482360703264273	23.997211383923855
45	23.786440110569604	29.078960411727138	25.264926652571912	21.869672825131346
46	21.23264630890261	29.683973095380097	27.352163533807094	21.7312170619102
47	20.713762210352005	31.01392845477193	25.436371288954902	22.83593804592116
48	21.305286832564406	29.71143673855424	27.069401763848422	21.91387466503293
49	21.327550259279544	28.76223472802055	26.59813214725063	23.312082865449273
50	20.82377928981294	29.92903329601095	25.84393825392982	23.403249160246293
51	20.231929654071852	28.23343771684496	27.973751907423143	23.560880721660038
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	19434.0
1	14506.0
2	9578.0
3	5903.5
4	2229.0
5	1873.0
6	1517.0
7	1498.0
8	1479.0
9	1633.5
10	1788.0
11	1863.0
12	1938.0
13	2079.0
14	2220.0
15	2335.5
16	2451.0
17	2328.0
18	2205.0
19	2216.5
20	2228.0
21	2572.5
22	2917.0
23	3406.5
24	3896.0
25	4355.0
26	5632.5
27	6451.0
28	7333.0
29	8215.0
30	8642.0
31	9069.0
32	11416.0
33	13763.0
34	14443.0
35	15123.0
36	19176.0
37	23229.0
38	22285.0
39	21341.0
40	22088.0
41	22835.0
42	26933.0
43	31031.0
44	32486.5
45	33942.0
46	37578.5
47	41215.0
48	44485.5
49	47756.0
50	47413.5
51	47071.0
52	44546.0
53	42021.0
54	39071.5
55	36122.0
56	32538.0
57	28954.0
58	27410.5
59	25867.0
60	25613.0
61	25359.0
62	23204.0
63	21049.0
64	18320.5
65	15592.0
66	13576.5
67	11561.0
68	10695.0
69	9829.0
70	8185.5
71	6542.0
72	5436.0
73	4330.0
74	3925.5
75	2892.0
76	2263.0
77	1646.5
78	1030.0
79	916.0
80	802.0
81	618.0
82	434.0
83	315.0
84	196.0
85	121.0
86	46.0
87	31.5
88	17.0
89	13.5
90	10.0
91	10.0
92	10.0
93	10.5
94	11.0
95	13.5
96	16.0
97	13.0
98	10.0
99	21.0
100	32.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	615359.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.85617287479971
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.46104799525226	17.70463104042643
2	9.200673316672134	4.205843597808301
3	3.395797303830449	2.3284479067238255
4	1.7387727671253577	1.5896676382164414
5	1.080555761609274	1.2348684644101213
6	0.720429943932991	0.9879762805628822
7	0.5756962624858968	0.9210749309127608
8	0.4517458848596044	0.8260145631864385
9	0.3445177918553741	0.7086922388181626
>10	3.2054520673804614	16.093804636377467
>50	0.9349504241509512	15.460423837117968
>100	0.8824493618717054	32.5672768700359
>500	0.0028767705358490802	0.45714053449677383
>1k	0.004315155803773621	2.14417156849188
>5k	0.0	0.0
>10k+	7.191926339622701E-4	2.769965892414648
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16851	2.738401485961853	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3758	0.6107004204049994	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3147	0.5114087873907751	No Hit
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT	1994	0.32403848810206726	TruSeq Adapter, Index 22 (95% over 23bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1723	0.2799991549648254	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGT	1356	0.22035917245055323	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCC	1066	0.17323221079077417	No Hit
AATCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT	894	0.14528104732359484	No Hit
CTGTCTGGGGCTCCTGTTTTTTAGCTGCTGTTCCTTGTCAGCTCTGACCAT	810	0.13163047911869333	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4059418973314764	0.0
2	0.0	0.0	0.0	0.565523539917349	0.0
3	0.0	0.0	0.0	0.7322554801343606	0.0
4	0.0	0.0	0.0	1.5418641800964965	0.0
5	0.0	0.0	0.0	1.6731696456865017	0.0
6	0.0	0.0	0.0	2.3764989217676185	0.0
7	0.0	0.0	0.0	2.9147213252751647	0.0
8	0.0	0.0	0.0	3.2275468466374915	0.0
9	0.0	0.0	0.0	3.976703030263635	0.0
10	0.0	0.0	0.0	4.896003796158015	0.0
11	0.0	0.0	0.0	5.938484689425198	0.0
12	0.0	0.0	0.0	6.404391582799634	0.0
13	0.0	0.0	0.0	6.592736922674407	0.0
14	0.0	0.0	0.0	6.81992137922741	0.0
15	0.0	0.0	0.0	7.0170420843767625	0.0
16	0.0	0.0	0.0	7.406733305273832	0.0
17	0.0	0.0	0.0	7.8737777459986775	0.0
18	0.0	0.0	0.0	8.57288184620685	0.0
19	0.0	0.0	0.0	8.826717412112279	0.0
20	0.0	0.0	0.0	9.113704357943899	0.0
21	0.0	0.0	0.0	9.397766182017326	0.0
22	0.0	0.0	0.0	9.716604453660384	0.0
23	0.0	0.0	0.0	10.044055583813677	0.0
24	0.0	0.0	0.0	10.27042750654496	0.0
25	0.0	0.0	0.0	10.484448915186094	0.0
26	0.0	0.0	0.0	10.684007221800607	0.0
27	0.0	0.0	0.0	10.907454022773697	0.0
28	0.0	0.0	0.0	11.114487640548038	0.0
29	0.0	0.0	0.0	11.32883406271786	0.0
30	0.0	0.0	0.0	11.598920305057698	0.0
31	0.0	0.0	0.0	11.810504112233671	0.0
32	0.0	0.0	0.0	12.043701319067406	0.0
33	0.0	0.0	0.0	12.279823647659335	0.0
34	0.0	0.0	0.0	12.495795137472598	0.0
35	0.0	0.0	0.0	12.738742750166976	0.0
36	0.0	0.0	0.0	12.955039253508927	0.0
37	0.0	0.0	0.0	13.181573683004554	0.0
38	0.0	0.0	0.0	13.422571214526805	0.0
39	0.0	0.0	0.0	13.645205481678175	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACAG	85	0.0	45.000004	1
TAGAGTC	20	7.030729E-4	45.0	38
AAACTCG	20	7.030729E-4	45.0	1
AGCCGTC	25	3.8886858E-5	45.0	13
GTCGCTA	20	7.030729E-4	45.0	33
TCGCATC	25	3.8886858E-5	45.0	15
TACCCTA	35	1.2107012E-7	45.0	19
GGTACGT	20	7.030729E-4	45.0	8
ACCGGTA	25	3.8886858E-5	45.0	32
AGATCAC	25	3.8886858E-5	45.0	37
TCCGCGT	25	3.8886858E-5	45.0	11
CTCGTCG	20	7.030729E-4	45.0	30
AGTTTCG	20	7.030729E-4	45.0	45
GATCGAG	20	7.030729E-4	45.0	1
CGAGTAT	25	3.8886858E-5	45.0	13
CAACGGC	20	7.030729E-4	45.0	16
TTTACCG	20	7.030729E-4	45.0	1
CCTAGTA	20	7.030729E-4	45.0	12
AGATACG	20	7.030729E-4	45.0	1
ACGCATG	25	3.8886858E-5	45.0	1
>>END_MODULE