##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553286_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	819235
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.664827552533765	31.0	31.0	34.0	30.0	34.0
2	31.861161937661354	33.0	31.0	34.0	30.0	34.0
3	32.03980176628196	33.0	31.0	34.0	30.0	34.0
4	35.74727520186516	37.0	35.0	37.0	35.0	37.0
5	35.50079403345804	37.0	35.0	37.0	33.0	37.0
6	34.1158471012591	37.0	35.0	37.0	32.0	37.0
7	34.990522865844355	37.0	35.0	37.0	32.0	37.0
8	35.70956807265315	37.0	35.0	37.0	33.0	37.0
9	37.4070455974171	39.0	37.0	39.0	35.0	39.0
10	36.77045902579846	39.0	37.0	39.0	32.0	39.0
11	36.76280066159283	39.0	37.0	39.0	32.0	39.0
12	36.79872930233694	39.0	37.0	39.0	32.0	39.0
13	36.7713488803579	39.0	37.0	39.0	33.0	39.0
14	37.93269452599071	40.0	37.0	41.0	33.0	41.0
15	37.916480619114175	40.0	37.0	41.0	33.0	41.0
16	37.86684040598851	40.0	37.0	41.0	33.0	41.0
17	37.8409735912162	39.0	37.0	41.0	33.0	41.0
18	37.75357864349057	39.0	37.0	41.0	33.0	41.0
19	37.74409174412714	39.0	36.0	41.0	33.0	41.0
20	37.63267560590063	39.0	36.0	41.0	33.0	41.0
21	37.60736540797207	39.0	36.0	41.0	33.0	41.0
22	37.60154778543397	39.0	36.0	41.0	33.0	41.0
23	37.43129749095193	39.0	36.0	41.0	32.0	41.0
24	37.26709186008898	39.0	36.0	41.0	32.0	41.0
25	37.301387269831004	39.0	36.0	41.0	32.0	41.0
26	37.24776651388186	39.0	36.0	41.0	32.0	41.0
27	37.183134265503796	39.0	35.0	41.0	32.0	41.0
28	37.12738347360647	39.0	35.0	40.0	32.0	41.0
29	36.96899058267774	39.0	35.0	40.0	31.0	41.0
30	36.971136487088565	39.0	35.0	40.0	31.0	41.0
31	36.93272382161407	39.0	35.0	40.0	31.0	41.0
32	36.83307231746691	39.0	35.0	40.0	31.0	41.0
33	36.788621091628166	39.0	35.0	40.0	31.0	41.0
34	36.695192466142196	39.0	35.0	40.0	31.0	41.0
35	36.570991229622756	39.0	35.0	40.0	30.0	41.0
36	36.515224569262784	39.0	35.0	40.0	30.0	41.0
37	36.48698847095156	39.0	35.0	40.0	30.0	41.0
38	36.37170897239498	38.0	35.0	40.0	30.0	41.0
39	36.290265918814505	38.0	35.0	40.0	30.0	41.0
40	36.19864263611784	38.0	35.0	40.0	30.0	41.0
41	36.10215872124604	38.0	35.0	40.0	30.0	41.0
42	36.02614756449615	38.0	35.0	40.0	30.0	41.0
43	35.931129651443115	38.0	35.0	40.0	29.0	41.0
44	35.807732823914996	38.0	35.0	40.0	29.0	41.0
45	35.575742003210316	38.0	34.0	40.0	28.0	41.0
46	35.381552301842575	38.0	34.0	40.0	27.0	41.0
47	35.29536823988233	38.0	34.0	40.0	27.0	41.0
48	35.193222945797	37.0	34.0	40.0	27.0	41.0
49	35.01602592662667	37.0	34.0	40.0	26.0	41.0
50	34.92430499185215	37.0	34.0	40.0	26.0	41.0
51	33.786303075430126	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	2.0
10	7.0
11	12.0
12	14.0
13	30.0
14	43.0
15	52.0
16	93.0
17	171.0
18	345.0
19	643.0
20	993.0
21	1590.0
22	2197.0
23	3023.0
24	3956.0
25	4904.0
26	6106.0
27	7622.0
28	9664.0
29	12487.0
30	16394.0
31	21345.0
32	28110.0
33	38376.0
34	55001.0
35	74556.0
36	85910.0
37	115536.0
38	163460.0
39	166567.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.978473820088254	31.636343662074985	20.277576031297492	27.10760648653927
2	22.973994031016744	31.908548829090556	20.631320683320414	24.48613645657229
3	21.513241011431397	32.274255860650484	19.750376875987964	26.462126251930158
4	20.682221828901355	33.2568798940475	18.59271149303924	27.468186784011912
5	18.398261793014214	35.69293304119087	15.9440209463707	29.964784219424217
6	18.34516347568158	41.214547718298164	18.03670497476304	22.403583831257208
7	86.78315745787228	6.492642526259254	3.822956782852295	2.9012432330161673
8	88.04848425665406	6.238502993646511	2.9713085988757806	2.741704150823634
9	81.04853918594786	10.306810622104768	5.331803450780301	3.3128467411670646
10	33.087941799361595	40.96065231588006	12.249110450603307	13.702295434155035
11	21.384828529054545	31.370730010314503	29.0751737901823	18.16926767044865
12	24.791665395155235	26.963081411316654	30.177787814241334	18.067465379286773
13	24.01227974879003	27.794832984430595	29.071633902360126	19.121253364419243
14	19.976075240925987	30.79519307646768	28.23438939986695	20.994342282739385
15	18.558777395985278	33.121021440734346	27.777988001000935	20.542213162279445
16	21.65349380824794	30.57925991931497	27.535810847925195	20.231435424511893
17	20.294787210019102	30.67862090853052	28.154192630930076	20.872399250520303
18	21.756272620188348	30.17253901505673	27.064761637381213	21.006426727373707
19	21.306218606382785	32.159392604075755	26.90265918814504	19.631729601396426
20	22.60352646066147	31.38928390510659	26.89033061331608	19.116859020915854
21	22.91198496158001	30.65176658712091	27.04999176060593	19.386256690693145
22	22.30764066476652	28.158709039530784	26.619712292565623	22.913938003137073
23	19.939455711731068	32.928524782266386	26.28940413922745	20.842615366775103
24	19.390651034196537	31.933572174040414	28.64013378334666	20.035643008416386
25	20.719085488290904	32.82330466837965	25.432873351358282	21.02473649197117
26	22.606822218289015	31.057755100795255	24.935824275085903	21.399598405829828
27	19.967042423724575	31.568597533064384	27.092226284277405	21.37213375893364
28	20.196402741582087	32.47151305791379	27.854522817018317	19.477561383485813
29	20.672212490921407	31.969703442846072	26.42697150390303	20.93111256232949
30	21.75578435979908	30.29863226058457	26.743486301244452	21.202097078371896
31	22.59839972657418	31.75023039787118	24.06073959242464	21.590630283129993
32	21.474302245387463	31.496945320939655	25.3399818123005	21.688770621372377
33	21.714404291808822	30.909690137750463	25.171287847809236	22.20461772263148
34	21.34796486966499	30.668977765842527	26.62117707373342	21.36188029075906
35	21.48663082021642	30.911276984015572	26.176493924209783	21.425598271558222
36	23.911820173698633	31.762436907602826	24.7854400751921	19.540302843506442
37	21.928140277209838	31.715808040427962	25.122828004174625	21.233223678187578
38	21.43743858599791	31.973609525960196	26.346530604771523	20.242421283270367
39	21.134350949361295	31.17664650558143	25.474253419348535	22.214749125708742
40	22.529432946590415	30.766507778598328	25.846185770871603	20.85787350393965
41	20.71823103260969	29.53426062118928	27.601481870281418	22.14602647591961
42	21.966956978156453	30.69656447783603	25.204855749571248	22.13162279443627
43	20.375960499734507	30.614414667342093	26.43978833912125	22.569836493802146
44	22.202176420685152	29.662428973371497	26.348361581231273	21.78703302471208
45	23.082998162920283	30.786770584752848	25.676759415796447	20.453471836530422
46	21.490536903330547	30.849634109870795	26.86164531544673	20.798183671351932
47	19.965211447264828	31.833600859338283	27.231136365023467	20.970051328373422
48	20.674897923062368	30.89699536762956	27.122864623703823	21.305242085604252
49	21.121534114143074	31.06739824348325	26.322849975892144	21.488217666481535
50	20.399519063516575	29.636795302935052	27.313652370809354	22.65003326273902
51	20.71932961848554	29.4413690821315	27.427661171702873	22.41164012768009
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	14276.0
1	10777.5
2	7279.0
3	4672.0
4	2065.0
5	1657.0
6	1249.0
7	1417.0
8	1585.0
9	1834.5
10	2084.0
11	2529.0
12	2974.0
13	3720.5
14	4467.0
15	4331.5
16	4196.0
17	4187.0
18	4178.0
19	4346.5
20	4515.0
21	4765.5
22	5016.0
23	5033.5
24	5051.0
25	5985.0
26	8476.5
27	10034.0
28	12225.0
29	14416.0
30	15690.5
31	16965.0
32	20436.0
33	23907.0
34	27360.0
35	30813.0
36	33386.0
37	35959.0
38	38704.0
39	41449.0
40	37747.5
41	34046.0
42	36408.0
43	38770.0
44	42530.5
45	46291.0
46	49626.5
47	52962.0
48	59102.5
49	65243.0
50	66420.0
51	67597.0
52	64624.0
53	61651.0
54	54921.0
55	48191.0
56	42693.0
57	37195.0
58	33504.0
59	29813.0
60	27243.0
61	24673.0
62	21601.0
63	18529.0
64	16603.5
65	14678.0
66	12898.5
67	11119.0
68	9498.0
69	7877.0
70	6834.0
71	5791.0
72	5376.0
73	4961.0
74	5253.5
75	3937.5
76	2329.0
77	1728.0
78	1127.0
79	895.0
80	663.0
81	527.0
82	391.0
83	301.5
84	212.0
85	168.0
86	124.0
87	81.5
88	39.0
89	25.5
90	12.0
91	8.5
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	819235.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.30653587722538
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.93121431116174	18.456247848555417
2	9.439169843352968	4.588670408973655
3	3.4304742424474224	2.5014883574983746
4	1.8234527482064253	1.7728727857881872
5	1.21435207120387	1.4758346093149906
6	0.8773582834075737	1.2795324355696236
7	0.6326753894771472	1.0764702937074673
8	0.4978482255176258	0.9680772603965728
9	0.43236081531961507	0.9458274302525376
>10	4.122498534956591	22.626412980974646
>50	0.970869396225498	16.740680987262344
>100	0.6200208260550558	23.967057466412612
>500	0.0041095000898429546	0.6874771735124814
>1k	0.0030821250673822164	1.3612197867114189
>5k	0.0	0.0
>10k+	5.136875112303693E-4	1.5521301750696797
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12431	1.517391224740154	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2847	0.34751933205978747	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2355	0.28746330418011923	No Hit
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT	2110	0.25755735533760155	Illumina PCR Primer Index 8 (95% over 24bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1328	0.16210244923617764	No Hit
AGAGAAGGAGAAGGAGAAGGAGAAGAAGAAAAGAAGAAGAAGAAGAAGAAG	1166	0.14232790347092106	No Hit
GTATGCGGGGAAGAAAAGGAAGAGAAAGAGGAAGAGAAAGAAAAAGAAGAA	1096	0.13378334665877312	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	897	0.10949239229280974	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	832	0.10155816096724384	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.31956642477433217	0.0
2	0.0	0.0	0.0	0.4258851245369155	0.0
3	0.0	0.0	0.0	0.5458751151989356	0.0
4	0.0	0.0	0.0	1.1569329923648282	0.0
5	0.0	0.0	0.0	1.2520217031743028	0.0
6	0.0	0.0	0.0	1.8367135193198534	0.0
7	0.0	0.0	0.0	2.25136865490366	0.0
8	0.0	0.0	0.0	2.626840894248903	0.0
9	0.0	0.0	0.0	3.2255701965858394	0.0
10	0.0	0.0	0.0	4.094063363992017	0.0
11	0.0	0.0	0.0	4.995392042576306	0.0
12	0.0	0.0	0.0	5.433605741942178	0.0
13	0.0	0.0	0.0	5.617557843597991	0.0
14	0.0	0.0	0.0	5.811397218136432	0.0
15	0.0	0.0	0.0	5.982898679865973	0.0
16	0.0	0.0	0.0	6.327122254298217	0.0
17	0.0	0.0	0.0	6.776932137909147	0.0
18	0.0	0.0	0.0	7.310600743376443	0.0
19	0.0	0.0	0.0	7.566205057156981	0.0
20	0.0	0.0	0.0	7.829011211679188	0.0
21	0.0	0.0	0.0	8.128558960493631	0.0
22	1.2206509731639884E-4	0.0	0.0	8.469852972590282	0.0
23	2.4413019463279768E-4	0.0	0.0	8.83702478531801	0.0
24	2.4413019463279768E-4	0.0	0.0	9.068094014537953	0.0
25	2.4413019463279768E-4	0.0	0.0	9.296721941811569	0.0
26	2.4413019463279768E-4	0.0	0.0	9.516072921689137	0.0
27	2.4413019463279768E-4	0.0	0.0	9.7606913767112	0.0
28	2.4413019463279768E-4	0.0	0.0	9.999938967451342	0.0
29	2.4413019463279768E-4	0.0	0.0	10.25053861224191	0.0
30	2.4413019463279768E-4	0.0	0.0	10.577428942855224	0.0
31	2.4413019463279768E-4	0.0	0.0	10.820704681806808	0.0
32	2.4413019463279768E-4	0.0	0.0	11.07582073519808	0.0
33	2.4413019463279768E-4	0.0	0.0	11.339725475596136	0.0
34	2.4413019463279768E-4	0.0	0.0	11.58837207882964	0.0
35	2.4413019463279768E-4	0.0	0.0	11.888041892741398	0.0
36	2.4413019463279768E-4	0.0	0.0	12.148895005706544	0.0
37	2.4413019463279768E-4	0.0	0.0	12.405964100654879	0.0
38	2.4413019463279768E-4	0.0	0.0	12.655465159569598	0.0
39	2.4413019463279768E-4	0.0	0.0	12.941341617484605	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTGC	55	1.8189894E-12	45.0	6
CTATGCG	35	1.211356E-7	45.0	1
AGGTACG	45	3.8380676E-10	45.0	1
GTCGCAA	20	7.0320995E-4	45.0	21
GCGACCG	50	2.1827873E-11	45.0	32
CGAGTTG	25	3.889821E-5	45.0	1
CTGTACG	20	7.0320995E-4	45.0	28
CGAAACG	40	6.8102963E-9	45.0	1
GCGAATC	25	3.889821E-5	45.0	28
CGACGAT	25	3.889821E-5	45.0	40
GTTTACG	20	7.0320995E-4	45.0	25
CGCATTA	55	1.8189894E-12	45.0	42
CGTTGTC	20	7.0320995E-4	45.0	45
TAGCGAT	20	7.0320995E-4	45.0	45
TAAACCG	50	2.1827873E-11	45.0	1
ATTCGGT	45	3.8380676E-10	45.0	40
GTTAGCG	45	3.8380676E-10	45.0	1
CGATTAC	20	7.0320995E-4	45.0	38
CGCGAGA	20	7.0320995E-4	45.0	23
CCTAACG	40	6.8102963E-9	45.0	1
>>END_MODULE