##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553281_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1698325
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.71078091649125	31.0	31.0	34.0	30.0	34.0
2	31.91361606288549	33.0	31.0	34.0	30.0	34.0
3	32.07926044779415	34.0	31.0	34.0	30.0	34.0
4	35.78789218789101	37.0	35.0	37.0	35.0	37.0
5	35.557873787408184	37.0	35.0	37.0	33.0	37.0
6	32.81850470316341	37.0	35.0	37.0	26.0	37.0
7	34.308705342028176	37.0	35.0	37.0	28.0	37.0
8	35.411568457156314	37.0	35.0	37.0	32.0	37.0
9	37.283707182076455	39.0	37.0	39.0	34.0	39.0
10	36.762245153312826	39.0	37.0	39.0	32.0	39.0
11	36.76504614841093	39.0	37.0	39.0	32.0	39.0
12	36.684271267278056	39.0	35.0	39.0	32.0	39.0
13	36.67010377872315	39.0	35.0	39.0	32.0	39.0
14	37.860148087085804	40.0	37.0	41.0	33.0	41.0
15	37.876855136678785	40.0	37.0	41.0	33.0	41.0
16	37.836864557725995	40.0	37.0	41.0	33.0	41.0
17	37.83029337729822	40.0	37.0	41.0	33.0	41.0
18	37.74237380948876	39.0	37.0	41.0	33.0	41.0
19	37.77792236468285	40.0	36.0	41.0	33.0	41.0
20	37.66407430850985	39.0	36.0	41.0	33.0	41.0
21	37.63355011555503	39.0	36.0	41.0	33.0	41.0
22	37.570780622083525	39.0	36.0	41.0	32.0	41.0
23	37.42997247287769	39.0	36.0	41.0	32.0	41.0
24	37.299853679360545	39.0	36.0	41.0	32.0	41.0
25	37.33496062296675	39.0	36.0	41.0	32.0	41.0
26	37.26409020652702	39.0	36.0	41.0	32.0	41.0
27	37.17260830524193	39.0	35.0	41.0	32.0	41.0
28	37.119205687957255	39.0	35.0	41.0	31.0	41.0
29	36.918161129348036	39.0	35.0	40.0	31.0	41.0
30	36.94132100746324	39.0	35.0	40.0	31.0	41.0
31	36.57318004504438	39.0	35.0	40.0	30.0	41.0
32	36.689304461749074	39.0	35.0	40.0	30.0	41.0
33	36.68478412553545	39.0	35.0	40.0	30.0	41.0
34	36.61150368745676	39.0	35.0	40.0	30.0	41.0
35	36.54957796652584	39.0	35.0	40.0	30.0	41.0
36	36.46018694890554	39.0	35.0	40.0	30.0	41.0
37	36.37456906069215	39.0	35.0	40.0	30.0	41.0
38	36.25534511945593	38.0	35.0	40.0	30.0	41.0
39	36.22143464884518	38.0	35.0	40.0	30.0	41.0
40	36.10641131703296	38.0	35.0	40.0	30.0	41.0
41	36.064643693050506	38.0	35.0	40.0	30.0	41.0
42	35.96441317180163	38.0	35.0	40.0	29.0	41.0
43	35.867067846260284	38.0	35.0	40.0	29.0	41.0
44	35.67441508545184	38.0	34.0	40.0	29.0	41.0
45	35.51201566249098	38.0	34.0	40.0	28.0	41.0
46	35.241927781784995	37.0	34.0	40.0	27.0	41.0
47	35.21224029558535	37.0	34.0	40.0	27.0	41.0
48	35.176951408004946	37.0	34.0	40.0	27.0	41.0
49	35.04219215992228	37.0	34.0	40.0	27.0	41.0
50	34.934161600400394	37.0	34.0	40.0	27.0	41.0
51	33.7470283956251	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	3.0
9	7.0
10	17.0
11	28.0
12	41.0
13	56.0
14	85.0
15	120.0
16	234.0
17	465.0
18	783.0
19	1380.0
20	2200.0
21	3233.0
22	4573.0
23	6352.0
24	8338.0
25	10927.0
26	13629.0
27	17266.0
28	21816.0
29	27695.0
30	35374.0
31	45553.0
32	59927.0
33	82377.0
34	119385.0
35	147641.0
36	177029.0
37	245051.0
38	330214.0
39	336469.0
40	54.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.850529197886154	29.41474688295821	20.31607613383775	28.418647785317887
2	25.169505247817703	29.462028763634756	19.389103970088176	25.979362018459362
3	22.078989592686913	30.50723506984823	19.611087394933243	27.802687942531612
4	21.091958252984558	32.545301988724184	18.992360119529536	27.370379638761722
5	19.14792516155624	34.15029514374457	16.498962212768465	30.202817481930726
6	17.977536690562758	43.67144097861128	17.00516685557829	21.34585547524767
7	87.36861319241017	5.388839003135442	3.833306345958518	3.409241458495871
8	88.48695037757791	4.933449133705269	3.460056231875525	3.119544256841299
9	82.4976373780048	7.772717236100275	5.777574963567044	3.9520704223278815
10	38.66185800715411	32.53664640160158	12.499668791309084	16.301826799935228
11	27.8410198283603	29.037316179176543	23.16488304653114	19.95678094593202
12	26.831907909263542	25.197002929356866	27.524472642162127	20.446616519217464
13	25.02495105471568	25.153312822928477	27.637525208661472	22.184210913694375
14	22.225192469050388	27.636288696215388	26.889552941869194	23.24896589286503
15	20.871211340585578	29.537927075206454	27.849086600032386	21.741774984175585
16	23.083567632814685	26.89985721225325	27.61373706446057	22.402838090471494
17	22.745293156492426	27.66302091766888	26.93394962683821	22.657736299000486
18	23.29030073749135	27.70323701293922	26.185506307685515	22.820955941883916
19	23.39022271944416	29.206129568839884	25.125461852119003	22.278185859596956
20	25.098376341395195	27.69505247817703	25.41886859111183	21.787702589315945
21	25.717810195339524	27.647476189775222	25.11810165898753	21.516611955897723
22	25.232331856387912	25.648447735268576	24.90983763413952	24.209382774203995
23	23.407710538324526	28.498314515772893	25.176688796314018	22.917286149588563
24	21.734002620228754	28.328971192204083	26.9691019092341	22.96792427833306
25	23.192145201890096	29.015058955146984	24.47323097758085	23.319564865382066
26	24.652819689988664	27.03557917359751	24.970838914813125	23.340762221600695
27	22.332592407224766	27.82094122149765	26.20593820381847	23.640528167459113
28	22.725273431174834	28.088911133028134	26.882251630282777	22.303563805514255
29	22.8357352096919	28.47399643766653	25.87578938071335	22.814478971928224
30	23.731794562289316	27.959960549364816	25.902403839076737	22.40584104926913
31	24.329972178469962	28.396272798198225	23.857565542519836	23.416189480811976
32	23.935171418898033	27.074146585606407	25.244932507028985	23.74574948846658
33	23.35754346194044	26.976167694640306	25.191232537941794	24.475056305477455
34	22.451120957413924	27.193793885151546	26.246448706814068	24.108636450620462
35	23.685160378608337	27.25320536410876	26.484035741097845	22.577598516185066
36	23.822766549394256	28.858963979214813	25.072821750842746	22.245447720548185
37	22.531199858684293	29.15825887271282	25.27631637054156	23.034224898061325
38	22.44800023552618	29.428230756775058	25.839341704326323	22.28442730337244
39	22.36114995657486	27.331576700572626	25.618418147292182	24.68885519556033
40	23.147395227650776	26.74511651185727	26.60857020888228	23.498918051609675
41	21.930313691431262	26.289255590066684	27.456464457627366	24.323966260874684
42	23.09451960019431	27.240310305742423	25.03896486243799	24.626205231625278
43	22.04507382273711	26.881721696377316	26.393181517083008	24.68002296380257
44	22.63824650758836	26.559934052669544	25.93802717383304	24.86379226590906
45	23.85738889788468	27.302901388132423	26.107782668217215	22.731927045765683
46	22.06132512917139	27.074735401056927	27.434619404413173	23.429320065358514
47	22.366390414084467	27.604728188067657	27.296601062811888	22.73228033503599
48	22.305624659591068	27.19426493751196	27.266807001015707	23.233303401881265
49	22.34554634713615	27.753109681598044	26.28213092311542	23.619213048150385
50	22.168666185800713	25.980834057085655	27.684277155432557	24.166222601681067
51	22.173023420134545	26.087762942899623	27.341704326321526	24.39750931064431
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13554.0
1	10525.5
2	7497.0
3	4933.5
4	2370.0
5	2161.0
6	1952.0
7	2094.5
8	2237.0
9	2382.0
10	2527.0
11	2828.5
12	3130.0
13	3582.0
14	4034.0
15	4157.0
16	4280.0
17	4006.5
18	3733.0
19	3901.0
20	4069.0
21	4661.5
22	5254.0
23	7506.0
24	9758.0
25	11600.0
26	14622.5
27	15803.0
28	19759.5
29	23716.0
30	27128.5
31	30541.0
32	35489.0
33	40437.0
34	46421.0
35	52405.0
36	57264.0
37	62123.0
38	63807.5
39	65492.0
40	67958.0
41	70424.0
42	76976.0
43	83528.0
44	87339.0
45	91150.0
46	99198.5
47	107247.0
48	111642.0
49	116037.0
50	119272.0
51	122507.0
52	116189.0
53	109871.0
54	104934.5
55	99998.0
56	94905.5
57	89813.0
58	87450.0
59	85087.0
60	81123.0
61	77159.0
62	71634.5
63	66110.0
64	60286.0
65	54462.0
66	49181.5
67	43901.0
68	38541.5
69	33182.0
70	29975.0
71	26768.0
72	22790.5
73	18813.0
74	16259.5
75	10983.5
76	8261.0
77	6860.0
78	5459.0
79	4273.5
80	3088.0
81	2424.5
82	1761.0
83	1351.5
84	942.0
85	602.5
86	263.0
87	202.5
88	142.0
89	169.0
90	196.0
91	120.0
92	44.0
93	34.0
94	24.0
95	18.0
96	12.0
97	9.5
98	7.0
99	8.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1698325.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.54397069348881
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.82358273237517	15.577174615292396
2	8.936081494291601	3.6716519266670864
3	3.427417930461497	2.1123832055321716
4	1.8703050149151388	1.5369396565720705
5	1.2185830094209722	1.2517266816563926
6	0.8227981915722765	1.0142125160589865
7	0.6557193283562388	0.9429755065433315
8	0.5157558393253815	0.8476538278477089
9	0.4036868092242531	0.7463996980245956
>10	3.9464100368748207	18.476764777047233
>50	1.396161346719773	20.771592099416562
>100	0.9739553547335738	30.605968958713138
>500	0.007229478582845856	0.9819693519674241
>1k	0.00202425400319684	0.7910292166160234
>5k	0.0	0.0
>10k+	2.8917914331383425E-4	0.6715579620448764
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11304	0.6655969852648933	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2592	0.15262096477411566	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	2387	0.14055024803850852	No Hit
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT	2279	0.13419104117292038	TruSeq Adapter, Index 22 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2215	0.13042262228960888	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1730528609070702	0.0
2	0.0	0.0	0.0	0.232699866044485	0.0
3	0.0	0.0	0.0	0.3054185741833866	0.0
4	0.0	0.0	0.0	0.6292670719679685	0.0
5	0.0	0.0	0.0	0.688972958650435	0.0
6	0.0	0.0	0.0	1.0707608967659312	0.0
7	0.0	0.0	0.0	1.3647564512092798	0.0
8	0.0	0.0	0.0	1.625954985058808	0.0
9	0.0	0.0	0.0	1.9857212253249525	0.0
10	5.888154505174216E-5	0.0	0.0	2.5559889891510754	0.0
11	5.888154505174216E-5	0.0	0.0	3.107355777015589	0.0
12	5.888154505174216E-5	0.0	0.0	3.360605302283132	0.0
13	5.888154505174216E-5	0.0	0.0	3.4737167503275286	0.0
14	5.888154505174216E-5	0.0	0.0	3.5963081271252557	0.0
15	5.888154505174216E-5	0.0	0.0	3.7057689193764443	0.0
16	5.888154505174216E-5	0.0	0.0	3.913267484138784	0.0
17	5.888154505174216E-5	0.0	0.0	4.169932139019328	0.0
18	5.888154505174216E-5	0.0	0.0	4.491248730366685	0.0
19	5.888154505174216E-5	0.0	0.0	4.639218053081713	0.0
20	5.888154505174216E-5	0.0	0.0	4.802849866780504	0.0
21	5.888154505174216E-5	0.0	0.0	4.995804689915063	0.0
22	5.888154505174216E-5	0.0	0.0	5.205599634934421	0.0
23	1.1776309010348432E-4	0.0	0.0	5.427583059779488	0.0
24	1.7664463515522648E-4	0.0	0.0	5.595689870902213	0.0
25	1.7664463515522648E-4	0.0	0.0	5.745131232243534	0.0
26	1.7664463515522648E-4	0.0	0.0	5.879322273416454	0.0
27	1.7664463515522648E-4	0.0	0.0	6.0202258107252735	0.0
28	1.7664463515522648E-4	0.0	0.0	6.166016516273387	0.0
29	1.7664463515522648E-4	0.0	0.0	6.319697348858434	0.0
30	1.7664463515522648E-4	0.0	0.0	6.519423549673943	0.0
31	1.7664463515522648E-4	0.0	0.0	6.668217214019696	0.0
32	1.7664463515522648E-4	0.0	0.0	6.848218097242872	0.0
33	1.7664463515522648E-4	0.0	0.0	7.017679183901786	0.0
34	1.7664463515522648E-4	0.0	0.0	7.175246198460248	0.0
35	2.3552618020696863E-4	0.0	0.0	7.359309908291993	0.0
36	2.3552618020696863E-4	0.0	0.0	7.519408829287681	0.0
37	2.3552618020696863E-4	0.0	0.0	7.684394918522662	0.0
38	2.3552618020696863E-4	0.0	0.0	7.857565542519835	0.0
39	2.3552618020696863E-4	0.0	0.0	8.035976624026615	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTCGC	20	7.034238E-4	45.000004	30
TCGTACC	20	7.034238E-4	45.000004	39
TCCCGAT	20	7.034238E-4	45.000004	38
GTATACG	20	7.034238E-4	45.000004	1
TTACGCT	40	6.8175723E-9	45.000004	15
TCGAACG	45	3.8562575E-10	45.000004	1
TAGACGC	25	3.8915954E-5	45.0	13
ATTACGC	35	1.2123928E-7	45.0	22
TTGCGAT	25	3.8915954E-5	45.0	16
CGCGTAG	55	1.8189894E-12	45.0	1
TAGTGCG	35	1.2123928E-7	45.0	1
TCGACCA	30	2.166118E-6	44.999996	18
CGGTCTA	95	0.0	42.63158	31
ATTACGG	75	0.0	42.0	2
CGGTAGT	190	0.0	41.44737	12
ACGCATG	50	1.0822987E-9	40.5	1
CATACCG	50	1.0822987E-9	40.5	1
CGATACG	45	1.9292202E-8	40.000004	1
TTCGTAC	85	0.0	39.705883	33
AATTCGT	85	0.0	39.705883	31
>>END_MODULE