FastQCFastQC Report
Sat 18 Jun 2016
SRR3553280_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553280_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380877
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA151323.97293614473964No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38651.0147632962872528No Hit
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT28400.7456475450079684TruSeq Adapter, Index 10 (95% over 24bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28330.7438096813406953No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15860.41640739661360493No Hit
AAACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCT6320.16593283395951974No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTT4920.12917556061405652No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC4850.12733769694678335RNA PCR Primer, Index 10 (95% over 23bp)
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3940.10344546927223223No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGG1500.045.0000042
CGGGTTC207.02734E-445.06
CGGGTTA207.02734E-445.06
CTAGACG207.02734E-445.01
CGGGTAC253.8858772E-545.06
TCGTTTC253.8858772E-545.030
CGGAACG253.8858772E-545.01
AACCGGA207.02734E-445.026
ACTATCG207.02734E-445.01
TTGGCGA351.209064E-745.027
AGTCCGG253.8858772E-545.02
GAAGCGG207.02734E-445.02
CAAACGA207.02734E-445.022
CGAACGA207.02734E-445.026
ACGCCCT207.02734E-445.040
TCCGCCT207.02734E-445.012
AGTTTCG207.02734E-445.021
GATCGGC207.02734E-445.028
CTTCCGA207.02734E-445.028
CAAAACG253.8858772E-545.01