##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553280_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	380877
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51012269052739	31.0	31.0	34.0	30.0	34.0
2	31.707154803256696	31.0	31.0	34.0	30.0	34.0
3	31.89971565623548	33.0	31.0	34.0	30.0	34.0
4	35.65087679224526	37.0	35.0	37.0	33.0	37.0
5	35.386379329809884	37.0	35.0	37.0	33.0	37.0
6	33.99155632920864	37.0	35.0	37.0	32.0	37.0
7	34.87264917545559	37.0	35.0	37.0	32.0	37.0
8	35.558140817114186	37.0	35.0	37.0	32.0	37.0
9	37.21677601955487	39.0	37.0	39.0	34.0	39.0
10	36.61963310990162	38.0	35.0	39.0	32.0	39.0
11	36.643050118542206	39.0	35.0	39.0	32.0	39.0
12	36.71951574917887	39.0	35.0	39.0	32.0	39.0
13	36.67872305232398	39.0	35.0	39.0	32.0	39.0
14	37.830239158573505	39.0	37.0	41.0	33.0	41.0
15	37.80187042010938	39.0	37.0	41.0	33.0	41.0
16	37.73787075617588	39.0	37.0	41.0	33.0	41.0
17	37.64129102046067	39.0	36.0	40.0	33.0	41.0
18	37.469445516531586	39.0	36.0	40.0	33.0	41.0
19	37.333960832499734	39.0	35.0	40.0	32.0	41.0
20	37.21768970034945	39.0	35.0	40.0	32.0	41.0
21	37.167463511842406	39.0	35.0	40.0	32.0	41.0
22	37.15623679035489	39.0	35.0	40.0	32.0	41.0
23	36.968974235776905	39.0	35.0	40.0	32.0	41.0
24	36.8011063939277	39.0	35.0	40.0	31.0	41.0
25	36.8872155577785	39.0	35.0	40.0	31.0	41.0
26	36.79910049701085	39.0	35.0	40.0	31.0	41.0
27	36.719983091654264	39.0	35.0	40.0	31.0	41.0
28	36.66482617747987	39.0	35.0	40.0	31.0	41.0
29	36.534369363337774	38.0	35.0	40.0	31.0	41.0
30	36.45514168616115	38.0	35.0	40.0	31.0	41.0
31	36.39564741373199	38.0	35.0	40.0	30.0	41.0
32	36.2509655348052	38.0	35.0	40.0	30.0	41.0
33	36.18357895068487	38.0	35.0	40.0	30.0	41.0
34	36.162356876366914	38.0	35.0	40.0	30.0	41.0
35	35.976050010895904	38.0	35.0	40.0	30.0	41.0
36	35.92557964907306	38.0	35.0	40.0	30.0	41.0
37	35.85333322831255	38.0	35.0	40.0	29.0	41.0
38	35.795949873581236	38.0	35.0	40.0	29.0	41.0
39	35.72707199437089	38.0	35.0	40.0	29.0	41.0
40	35.58530181659696	38.0	35.0	40.0	28.0	41.0
41	35.494684635722294	38.0	35.0	40.0	28.0	41.0
42	35.39107900975905	38.0	34.0	40.0	27.0	41.0
43	35.31865142815135	38.0	34.0	40.0	27.0	41.0
44	35.150213848565286	38.0	34.0	40.0	26.0	41.0
45	35.02015086235189	38.0	34.0	40.0	26.0	41.0
46	34.82494873673128	37.0	33.0	40.0	25.0	41.0
47	34.76134815176553	37.0	33.0	40.0	25.0	41.0
48	34.644257857523556	37.0	33.0	40.0	24.0	41.0
49	34.468248279628334	37.0	33.0	40.0	24.0	41.0
50	34.414551154309656	37.0	33.0	40.0	24.0	41.0
51	33.28594270591293	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	3.0
12	0.0
13	5.0
14	10.0
15	17.0
16	46.0
17	113.0
18	272.0
19	414.0
20	781.0
21	1191.0
22	1681.0
23	2167.0
24	2662.0
25	3006.0
26	3530.0
27	4149.0
28	5164.0
29	6409.0
30	8298.0
31	10771.0
32	14053.0
33	19078.0
34	27371.0
35	41220.0
36	41008.0
37	51391.0
38	70599.0
39	65454.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.206260813858542	33.81039023096695	19.7034738248831	26.27987513029141
2	21.05745424375848	35.842542343066135	19.078337626057756	24.021665787117627
3	18.94469868225175	36.894588016603784	19.30386975322742	24.856843547917045
4	18.429046647605396	38.651060578612	18.20587748800794	24.714015285774675
5	15.806152642454125	40.47973492754879	17.312675745713182	26.4014366842839
6	17.90998143757696	43.49068071844716	17.501713151489852	21.097624692486026
7	78.69102098577756	12.46465394339905	4.917335517765578	3.9269895530578114
8	80.15186004930726	12.241484783801594	3.7263998613725686	3.88025530551858
9	72.58196215576157	16.539722797648583	7.241445401008724	3.636869645581119
10	26.73907849515723	47.70595231531439	12.05743586512181	13.497533324406566
11	17.878737755233317	34.17323702927717	31.93997012158781	16.008055093901703
12	21.05351596447147	29.732958409145212	32.10511529968992	17.108410326693395
13	21.263032422540608	31.480766756721984	28.560663941377403	18.695536879360002
14	16.703292664035896	35.247599618774565	28.57825492219273	19.47085279499681
15	15.07888373411889	38.27771170220307	27.939203469886603	18.704201093791433
16	18.371285218062525	35.336079626756145	27.059129325215224	19.233505829966106
17	16.722984060470967	34.36306209091124	29.243561569745612	19.670392278872182
18	17.29219669342071	34.6445177839565	27.576094119623924	20.48719140299887
19	17.52928110649895	37.50554640999588	27.64908356240991	17.316088921095265
20	17.89921680752579	36.85179204835157	27.533298151371703	17.715692992750938
21	18.58815313080076	36.45140032083848	26.712035644053067	18.24841090430769
22	16.630303221249903	34.97769621163788	26.655061870367597	21.736938696744616
23	16.246977370647215	36.43906037907251	26.40196178818884	20.912000462091438
24	15.63969470458967	36.69872426006296	26.62434329192889	21.03723774341848
25	16.76630513262812	36.62179653798995	25.806493959992334	20.805404369389592
26	18.573975325367506	36.136337977877375	25.331274926025987	19.958411770729136
27	15.94845580069156	36.13870094544958	26.555292128429915	21.357551125428945
28	17.16879727576094	35.46394242760786	28.75180176277381	18.61545853385739
29	18.670069339970645	36.21379080385531	25.261698658622073	19.854441197551967
30	17.89659128800111	37.19862317756126	26.00340792434303	18.901377610094595
31	18.644601800581288	36.276015616590136	24.30600955164003	20.773373031188548
32	18.652740911107784	35.754849990941956	25.20603764469894	20.38637145325131
33	17.248875621263558	36.00768752116825	27.031823922158598	19.711612935409594
34	16.531321135169623	33.359063424675156	28.314127658010328	21.795487782144892
35	17.62064918595767	34.60776051061104	26.852763490575697	20.918826812855592
36	18.734657120277674	36.06991233390307	26.721224962389435	18.47420558342982
37	16.960856129406608	36.48868269808889	25.812795206851558	20.737665965652955
38	17.258064939599922	34.912583327425914	28.65649540402807	19.17285632894609
39	18.133150597174417	33.05292784809794	26.82020704846972	21.993714506257923
40	18.509650097012948	34.19450373742705	25.33678851702781	21.959057648532205
41	16.956655298167124	32.31043092651959	28.172612155630294	22.560301619682996
42	19.69664747411894	33.028773068470926	25.406627336384187	21.86795212102595
43	17.84460600141253	32.568782047747696	27.094048734893416	22.492563215946358
44	19.238756869015454	32.89828474809453	26.074559503461746	21.788398879428268
45	20.82220769434752	32.08594900715979	28.01613119195961	19.07571210653308
46	17.096070384927415	33.83060673130696	29.519503671789053	19.55381921197657
47	18.016052426373868	33.63946890991055	26.404587307713513	21.939891356002068
48	18.50072333062905	31.505971744158874	29.445464021193196	20.547840904018884
49	18.269940164410034	31.23580578506972	28.033197068870003	22.461056981650245
50	17.99504827017646	30.255436794555724	27.52410883303534	24.22540610223248
51	17.750349850476663	30.936496559256664	29.789932182830675	21.523221407435997
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	17133.0
1	12992.0
2	8851.0
3	5338.0
4	1825.0
5	1595.0
6	1365.0
7	1387.0
8	1409.0
9	1664.5
10	1920.0
11	2280.0
12	2640.0
13	2772.0
14	2904.0
15	3042.5
16	3181.0
17	3092.5
18	3004.0
19	3067.5
20	3131.0
21	3041.5
22	2952.0
23	2786.0
24	2620.0
25	2829.5
26	4114.0
27	5189.0
28	5762.5
29	6336.0
30	7081.0
31	7826.0
32	8876.0
33	9926.0
34	10849.5
35	11773.0
36	12726.5
37	13680.0
38	15256.5
39	16833.0
40	18302.0
41	19771.0
42	21583.5
43	23396.0
44	26730.0
45	30064.0
46	33666.5
47	37269.0
48	35894.5
49	34520.0
50	31034.0
51	27548.0
52	23893.5
53	20239.0
54	17609.0
55	14979.0
56	13362.5
57	11746.0
58	10393.5
59	9041.0
60	8042.0
61	7043.0
62	6374.5
63	5706.0
64	4669.5
65	3633.0
66	3257.5
67	2882.0
68	2382.0
69	1882.0
70	1696.0
71	1510.0
72	1243.0
73	976.0
74	796.5
75	430.5
76	244.0
77	179.0
78	114.0
79	96.0
80	78.0
81	53.5
82	29.0
83	25.5
84	22.0
85	20.0
86	18.0
87	12.0
88	6.0
89	5.0
90	4.0
91	2.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	380877.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.089956162362697
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.11443451521991	23.66394432401213
2	9.80688614795757	6.097913208585657
3	3.783238358000935	3.528621441060543
4	2.070346778790667	2.5746796237396263
5	1.2474086890671154	1.9390940729823472
6	0.8636604485194874	1.6110699290182422
7	0.671895575153248	1.4622442784039966
8	0.5259744770570636	1.3082018747380602
9	0.4275656580790255	1.1963697809587797
>10	3.5952828782512025	23.843777714103258
>50	0.626078041539417	13.520344766021742
>100	0.2621383669860161	12.16203304301031
>500	8.483442297282074E-4	0.16668990464916975
>1k	0.0033933769189128295	2.9339533217046903
>5k	0.0	0.0
>10k+	8.483442297282074E-4	3.99106271701145
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15132	3.97293614473964	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3865	1.0147632962872528	No Hit
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	2840	0.7456475450079684	TruSeq Adapter, Index 10 (95% over 24bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2833	0.7438096813406953	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1586	0.41640739661360493	No Hit
AAACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCT	632	0.16593283395951974	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTT	492	0.12917556061405652	No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	485	0.12733769694678335	RNA PCR Primer, Index 10 (95% over 23bp)
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	394	0.10344546927223223	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.625519524675945E-4	0.0	0.0	0.856707020901761	0.0
2	2.625519524675945E-4	0.0	0.0	1.1709817080054716	0.0
3	2.625519524675945E-4	0.0	0.0	1.4855189470616499	0.0
4	2.625519524675945E-4	0.0	0.0	3.024598492426689	0.0
5	2.625519524675945E-4	0.0	0.0	3.2359528141631024	0.0
6	2.625519524675945E-4	0.0	0.0	4.523507589064186	0.0
7	2.625519524675945E-4	0.0	0.0	5.474733312854281	0.0
8	2.625519524675945E-4	0.0	0.0	6.27814228740512	0.0
9	2.625519524675945E-4	0.0	0.0	7.44387295636124	0.0
10	2.625519524675945E-4	0.0	0.0	9.365228144519097	0.0
11	2.625519524675945E-4	0.0	0.0	11.060263549649887	0.0
12	2.625519524675945E-4	0.0	0.0	11.915920362741778	0.0
13	2.625519524675945E-4	0.0	0.0	12.274828881764979	0.0
14	2.625519524675945E-4	0.0	0.0	12.606169445779084	0.0
15	2.625519524675945E-4	0.0	0.0	12.906528879402012	0.0
16	2.625519524675945E-4	0.0	0.0	13.524838727463196	0.0
17	2.625519524675945E-4	0.0	0.0	14.446921184529389	0.0
18	2.625519524675945E-4	0.0	0.0	15.549376832940817	0.0
19	5.25103904935189E-4	0.0	0.0	15.987838593561701	0.0
20	5.25103904935189E-4	0.0	0.0	16.502440420398187	0.0
21	5.25103904935189E-4	0.0	0.0	17.008903136708177	0.0
22	5.25103904935189E-4	0.0	0.0	17.603583309047277	0.0
23	5.25103904935189E-4	0.0	0.0	18.20482728019807	0.0
24	5.25103904935189E-4	0.0	0.0	18.596029689374785	0.0
25	5.25103904935189E-4	0.0	0.0	18.950999929110974	0.0
26	5.25103904935189E-4	0.0	0.0	19.259498473260397	0.0
27	5.25103904935189E-4	0.0	0.0	19.63652307700386	0.0
28	5.25103904935189E-4	0.0	0.0	20.02089913541642	0.0
29	5.25103904935189E-4	0.0	0.0	20.408950921163527	0.0
30	5.25103904935189E-4	0.0	0.0	20.891258857846495	0.0
31	5.25103904935189E-4	0.0	0.0	21.248329513202425	0.0
32	5.25103904935189E-4	0.0	0.0	21.592797674839908	0.0
33	5.25103904935189E-4	0.0	0.0	21.94829301848103	0.0
34	5.25103904935189E-4	0.0	0.0	22.29144842035618	0.0
35	5.25103904935189E-4	0.0	0.0	22.713106856019134	0.0
36	5.25103904935189E-4	0.0	0.0	23.084355316808313	0.0
37	5.25103904935189E-4	0.0	0.0	23.44378893973645	0.0
38	5.25103904935189E-4	0.0	0.0	23.80847360171394	0.0
39	5.25103904935189E-4	0.0	0.0	24.169745088309348	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACACGG	150	0.0	45.000004	2
CGGGTTC	20	7.02734E-4	45.0	6
CGGGTTA	20	7.02734E-4	45.0	6
CTAGACG	20	7.02734E-4	45.0	1
CGGGTAC	25	3.8858772E-5	45.0	6
TCGTTTC	25	3.8858772E-5	45.0	30
CGGAACG	25	3.8858772E-5	45.0	1
AACCGGA	20	7.02734E-4	45.0	26
ACTATCG	20	7.02734E-4	45.0	1
TTGGCGA	35	1.209064E-7	45.0	27
AGTCCGG	25	3.8858772E-5	45.0	2
GAAGCGG	20	7.02734E-4	45.0	2
CAAACGA	20	7.02734E-4	45.0	22
CGAACGA	20	7.02734E-4	45.0	26
ACGCCCT	20	7.02734E-4	45.0	40
TCCGCCT	20	7.02734E-4	45.0	12
AGTTTCG	20	7.02734E-4	45.0	21
GATCGGC	20	7.02734E-4	45.0	28
CTTCCGA	20	7.02734E-4	45.0	28
CAAAACG	25	3.8858772E-5	45.0	1
>>END_MODULE