SRR3553279_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553279_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	377573
Sequences flagged as poor quality	0
Sequence length	51
%GC	41

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15396	4.077622075731051	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3672	0.9725271669319576	No Hit
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	2727	0.7222444401479978	TruSeq Adapter, Index 10 (95% over 24bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2675	0.7084722689387218	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1556	0.41210573849295373	No Hit
AAACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCT	567	0.15016963607037578	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTT	483	0.12792228257846827	No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	473	0.12527378811514595	RNA PCR Primer, Index 10 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	60	0.0	45.000004	21
TCCGGAT	30	2.1616142E-6	45.000004	4
GATCGAA	30	2.1616142E-6	45.000004	9
TTTACGG	30	2.1616142E-6	45.000004	2
TTCACCC	30	2.1616142E-6	45.000004	41
CGCTGCG	30	2.1616142E-6	45.000004	1
GCACCGT	30	2.1616142E-6	45.000004	24
TGACCGG	30	2.1616142E-6	45.000004	2
CGGATGT	30	2.1616142E-6	45.000004	6
AGCATAG	30	2.1616142E-6	45.000004	1
GACGGGT	30	2.1616142E-6	45.000004	4
GATGCGT	30	2.1616142E-6	45.000004	18
CTAGGTC	30	2.1616142E-6	45.000004	11
GTTGATC	55	1.8189894E-12	45.0	16
CCTTATG	40	6.7939254E-9	45.0	21
GATACTC	20	7.0272625E-4	45.0	28
TGATAGA	25	3.8858132E-5	45.0	40
TTGGCGA	20	7.0272625E-4	45.0	27
ACCGGGT	75	0.0	45.0	4
CGAACCT	20	7.0272625E-4	45.0	35