##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553279_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	377573
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49210086526314	31.0	31.0	34.0	30.0	34.0
2	31.696567286326086	31.0	31.0	34.0	30.0	34.0
3	31.886437854401667	33.0	31.0	34.0	30.0	34.0
4	35.64571884112476	37.0	35.0	37.0	33.0	37.0
5	35.40093968583559	37.0	35.0	37.0	33.0	37.0
6	32.6038487921541	37.0	35.0	37.0	26.0	37.0
7	34.18539990942149	37.0	35.0	37.0	28.0	37.0
8	35.275724164598635	37.0	35.0	37.0	32.0	37.0
9	37.11317016841776	39.0	37.0	39.0	34.0	39.0
10	36.59674023301454	38.0	35.0	39.0	32.0	39.0
11	36.66109335148435	39.0	35.0	39.0	32.0	39.0
12	36.72580136821224	39.0	35.0	39.0	32.0	39.0
13	36.71507231714132	39.0	35.0	39.0	32.0	39.0
14	37.8379174358336	39.0	37.0	41.0	33.0	41.0
15	37.84970853318431	39.0	37.0	41.0	33.0	41.0
16	37.816766559049505	39.0	37.0	41.0	33.0	41.0
17	37.74511948682771	39.0	37.0	41.0	33.0	41.0
18	37.5661474734687	39.0	36.0	40.0	33.0	41.0
19	37.44239127268104	39.0	35.0	40.0	32.0	41.0
20	37.30939977169978	39.0	35.0	40.0	32.0	41.0
21	37.23527370866031	39.0	35.0	40.0	32.0	41.0
22	37.18691484825451	39.0	35.0	40.0	32.0	41.0
23	36.99434281582634	39.0	35.0	40.0	32.0	41.0
24	36.91640027226523	39.0	35.0	40.0	31.0	41.0
25	36.95758965815882	39.0	35.0	40.0	31.0	41.0
26	36.87057602106083	39.0	35.0	40.0	31.0	41.0
27	36.75376682125046	39.0	35.0	40.0	31.0	41.0
28	36.74310663103559	39.0	35.0	40.0	31.0	41.0
29	36.57676793626663	39.0	35.0	40.0	31.0	41.0
30	36.50723965961549	38.0	35.0	40.0	31.0	41.0
31	36.1339131770545	38.0	35.0	40.0	30.0	41.0
32	36.20489018017708	38.0	35.0	40.0	30.0	41.0
33	36.16356572106586	38.0	35.0	40.0	30.0	41.0
34	36.19215621879742	38.0	35.0	40.0	30.0	41.0
35	36.09130154963411	38.0	35.0	40.0	30.0	41.0
36	36.0233597211665	38.0	35.0	40.0	30.0	41.0
37	35.924300731249325	38.0	35.0	40.0	30.0	41.0
38	35.852447076459384	38.0	35.0	40.0	29.0	41.0
39	35.73272982972829	38.0	35.0	40.0	29.0	41.0
40	35.64454555807751	38.0	35.0	40.0	28.0	41.0
41	35.61703829458145	38.0	35.0	40.0	29.0	41.0
42	35.514199903065105	38.0	35.0	40.0	28.0	41.0
43	35.46223114470579	38.0	35.0	40.0	28.0	41.0
44	35.25280144501858	38.0	34.0	40.0	27.0	41.0
45	35.08764662727472	38.0	34.0	40.0	26.0	41.0
46	34.7919554629171	37.0	33.0	40.0	24.0	41.0
47	34.79345451078335	37.0	33.0	40.0	25.0	41.0
48	34.80992020086182	37.0	34.0	40.0	25.0	41.0
49	34.68017575409259	37.0	33.0	40.0	25.0	41.0
50	34.576044897278145	37.0	33.0	40.0	24.0	41.0
51	33.515203682466705	36.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	3.0
12	1.0
13	1.0
14	9.0
15	25.0
16	41.0
17	111.0
18	237.0
19	460.0
20	765.0
21	1116.0
22	1617.0
23	2139.0
24	2639.0
25	2955.0
26	3360.0
27	4035.0
28	5025.0
29	6325.0
30	8143.0
31	10649.0
32	13954.0
33	18789.0
34	27350.0
35	41199.0
36	41131.0
37	53390.0
38	68805.0
39	63287.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.13173611460565	33.75161889224071	19.835104734713553	26.28154025844009
2	21.025602995976936	35.78884083342824	19.097763876124617	24.08779229447021
3	18.97010644299248	36.78096685938878	19.360759376332524	24.888167321286215
4	18.53019151263464	38.665635519488944	18.21899341319427	24.585179554682142
5	15.69577273798709	40.66154094704865	17.22395404332407	26.418732271640188
6	17.283280319302495	45.75009335942983	16.759143265011005	20.20748305625667
7	78.7749653709349	12.418525689072046	4.851512157913834	3.954996782079227
8	80.00889894139677	12.357345466969301	3.6943849268883104	3.9393706647456255
9	72.5523276293591	16.506212043763725	7.277268236870752	3.664192090006436
10	26.79746697989528	47.69594224163274	12.035023690782975	13.471567087689005
11	17.79761794407969	34.2987978483631	31.94428627046955	15.959297937087664
12	21.075394691887396	29.815426420851065	32.0136768254086	17.09550206185294
13	21.224769779618775	31.39710731434716	28.602680806095776	18.77544209993829
14	16.727096482004804	35.28085959536302	28.490649490297244	19.50139443233494
15	15.207920057843118	38.251146135979006	27.958566952615787	18.582366853562093
16	18.521186631459347	35.34680710749974	27.115021466047622	19.016984794993284
17	16.75596507165502	34.49425673975629	29.165485879551767	19.584292309036925
18	17.464967039486403	34.66190643928459	27.489518583161406	20.383607938067605
19	17.68797027329814	37.530490792509	27.65981677715303	17.12172215703983
20	17.964737944715324	36.930871646012825	27.314453099135793	17.789937310136054
21	18.479340418938854	36.50578828464959	26.79137544262964	18.22349585378192
22	16.722329191970825	34.77314320674413	26.756680165160112	21.747847436124935
23	16.24003835019983	36.352970154115894	26.52043445903176	20.886557036652516
24	15.799858570395658	36.72349452953468	26.58240922947351	20.894237670596148
25	16.75358142663803	36.62602993328443	25.729858861730047	20.8905297783475
26	18.506619911911073	36.19326593797756	25.49043496224571	19.809679187865658
27	15.92804570242046	36.23802549440771	26.523877501834082	21.310051301337754
28	17.272951190895537	35.51842954872303	28.691405370617073	18.517213889764363
29	18.53575335100762	36.30132451208111	25.33126044500004	19.831661691911233
30	17.955997912986362	37.07124185256891	26.060920669645338	18.91183956479939
31	18.77279360547497	36.33681433788963	24.197704814698085	20.69268724193732
32	18.66499988081775	35.74540552422975	25.20042481851192	20.389169776440582
33	17.271626943663872	35.97847303700212	26.988688280147148	19.76121173918686
34	16.488467130859462	33.4886233920328	28.40166007632961	21.62124940077813
35	17.42523962253657	34.73632913370395	26.88910488832623	20.949326355433254
36	18.72326675901084	36.06931639709407	26.6480918921639	18.55932495173119
37	17.05286130099345	36.454142642614805	25.976433696265357	20.516562360126386
38	17.318770145111014	34.982639118792925	28.53779269174438	19.160798044351687
39	18.157283492198857	33.175571346468104	26.631936075937634	22.035209085395408
40	18.626067012206914	34.11022504257455	25.26266443839999	22.001043506818547
41	16.98400044494707	32.378639362454415	27.995645875102298	22.64171431749622
42	19.558866762189034	33.2560855781531	25.348210809565302	21.836836850092563
43	17.819070749232598	32.53728418080742	27.23076067409481	22.41288439586517
44	19.376385493666128	32.92528861968414	26.016955661554192	21.681370225095545
45	20.612702709144987	32.262635304960895	28.005180455170258	19.11948153072386
46	17.211770968792788	33.81889065160909	29.29287846323757	19.676459916360546
47	17.973742825890625	33.757710429506346	26.41767287385486	21.85087387074817
48	18.3564502758407	31.649508836701777	29.295262108254562	20.69877877920296
49	18.305069483252247	31.350493811792685	27.827731326127665	22.516705378827407
50	17.94619848347207	30.307781541582685	27.687890818464243	24.058129156480998
51	17.62043366448342	31.15635916763116	29.73756068362939	21.485646484256023
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	17374.0
1	12959.5
2	8545.0
3	5205.0
4	1865.0
5	1596.5
6	1328.0
7	1373.5
8	1419.0
9	1639.0
10	1859.0
11	2225.5
12	2592.0
13	2803.0
14	3014.0
15	3058.5
16	3103.0
17	3058.0
18	3013.0
19	2964.0
20	2915.0
21	2928.5
22	2942.0
23	2784.5
24	2627.0
25	2843.0
26	4096.0
27	5133.0
28	5788.5
29	6444.0
30	7170.5
31	7897.0
32	8797.0
33	9697.0
34	10658.0
35	11619.0
36	12777.0
37	13935.0
38	15304.0
39	16673.0
40	18057.5
41	19442.0
42	21402.0
43	23362.0
44	26690.0
45	30018.0
46	33500.0
47	36982.0
48	35387.0
49	33792.0
50	30304.0
51	26816.0
52	23513.0
53	20210.0
54	17491.5
55	14773.0
56	13128.0
57	11483.0
58	10290.0
59	9097.0
60	8061.0
61	7025.0
62	6299.5
63	5574.0
64	4582.0
65	3590.0
66	3281.0
67	2972.0
68	2394.0
69	1816.0
70	1662.0
71	1508.0
72	1227.5
73	947.0
74	792.0
75	437.5
76	238.0
77	164.5
78	91.0
79	88.0
80	85.0
81	52.5
82	20.0
83	19.0
84	18.0
85	14.5
86	11.0
87	8.0
88	5.0
89	2.5
90	0.0
91	1.0
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	377573.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.523840686279993
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.81062173934161	23.898419620388395
2	9.944656374240708	6.269875264427258
3	3.7728334273316255	3.5680259969722163
4	2.0629326866704227	2.6012624544447185
5	1.3459637271872642	2.1214973052681474
6	0.8954014214572651	1.6935895056172483
7	0.6600946005846566	1.4566101918693035
8	0.5339946264884576	1.346684922620138
9	0.44598201351572886	1.2653159348714587
>10	3.6736376976625413	24.34538267027853
>50	0.6143886062312188	13.35450595783641
>100	0.234415487501443	10.98447397698544
>500	8.462652978391445E-4	0.15126160886340606
>1k	0.003385061191356578	2.835821697033521
>5k	0.0	0.0
>10k+	8.462652978391445E-4	4.107272892523809
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15396	4.077622075731051	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3672	0.9725271669319576	No Hit
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	2727	0.7222444401479978	TruSeq Adapter, Index 10 (95% over 24bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2675	0.7084722689387218	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1556	0.41210573849295373	No Hit
AAACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCT	567	0.15016963607037578	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTT	483	0.12792228257846827	No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	473	0.12527378811514595	RNA PCR Primer, Index 10 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8287139175735553	0.0
2	0.0	0.0	0.0	1.1306422863923002	0.0
3	0.0	0.0	0.0	1.4341597518890388	0.0
4	0.0	0.0	0.0	2.8675249554390807	0.0
5	0.0	0.0	0.0	3.08337725419985	0.0
6	0.0	0.0	0.0	4.3286993508540075	0.0
7	0.0	0.0	0.0	5.245608134056196	0.0
8	0.0	0.0	0.0	6.057106837618156	0.0
9	0.0	0.0	0.0	7.216352864214337	0.0
10	0.0	0.0	0.0	9.08645480476623	0.0
11	0.0	0.0	0.0	10.736466855416039	0.0
12	0.0	0.0	0.0	11.57550990139655	0.0
13	0.0	0.0	0.0	11.931997256159736	0.0
14	0.0	0.0	0.0	12.24213595781478	0.0
15	0.0	0.0	0.0	12.55174496057716	0.0
16	0.0	0.0	0.0	13.159839289355965	0.0
17	0.0	0.0	0.0	14.020070291043057	0.0
18	0.0	0.0	0.0	15.129524621728779	0.0
19	0.0	0.0	0.0	15.601486335092817	0.0
20	0.0	0.0	0.0	16.1025814875534	0.0
21	0.0	0.0	0.0	16.616654262884264	0.0
22	0.0	0.0	0.0	17.183696927481574	0.0
23	0.0	0.0	0.0	17.74438320536691	0.0
24	0.0	0.0	0.0	18.117556075249023	0.0
25	0.0	0.0	0.0	18.48119436506318	0.0
26	0.0	0.0	0.0	18.7976894534302	0.0
27	0.0	0.0	0.0	19.179602355041276	0.0
28	0.0	0.0	0.0	19.54191639762377	0.0
29	0.0	0.0	0.0	19.90608438633059	0.0
30	0.0	0.0	0.0	20.391818270903904	0.0
31	0.0	0.0	0.0	20.72738251940684	0.0
32	0.0	0.0	0.0	21.062417069017116	0.0
33	0.0	0.0	0.0	21.41863957433397	0.0
34	0.0	0.0	0.0	21.761089908441548	0.0
35	0.0	0.0	0.0	22.198886043228725	0.0
36	0.0	0.0	0.0	22.561729784703886	0.0
37	0.0	0.0	0.0	22.909741957184437	0.0
38	0.0	0.0	0.0	23.254840785755338	0.0
39	0.0	0.0	0.0	23.61477118332084	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	60	0.0	45.000004	21
TCCGGAT	30	2.1616142E-6	45.000004	4
GATCGAA	30	2.1616142E-6	45.000004	9
TTTACGG	30	2.1616142E-6	45.000004	2
TTCACCC	30	2.1616142E-6	45.000004	41
CGCTGCG	30	2.1616142E-6	45.000004	1
GCACCGT	30	2.1616142E-6	45.000004	24
TGACCGG	30	2.1616142E-6	45.000004	2
CGGATGT	30	2.1616142E-6	45.000004	6
AGCATAG	30	2.1616142E-6	45.000004	1
GACGGGT	30	2.1616142E-6	45.000004	4
GATGCGT	30	2.1616142E-6	45.000004	18
CTAGGTC	30	2.1616142E-6	45.000004	11
GTTGATC	55	1.8189894E-12	45.0	16
CCTTATG	40	6.7939254E-9	45.0	21
GATACTC	20	7.0272625E-4	45.0	28
TGATAGA	25	3.8858132E-5	45.0	40
TTGGCGA	20	7.0272625E-4	45.0	27
ACCGGGT	75	0.0	45.0	4
CGAACCT	20	7.0272625E-4	45.0	35
>>END_MODULE