FastQCFastQC Report
Sat 18 Jun 2016
SRR3553278_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553278_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1413853
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA113870.8053878302765562No Hit
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT25110.17759979290633468TruSeq Adapter, Index 16 (95% over 22bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25100.17752906419549982No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20790.14704498982567496No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAT406.8175723E-945.034
CGCATAG750.045.01
GCACGAC207.033838E-445.028
ATGTCGA207.033838E-445.038
CCCGTTA351.2121927E-745.044
CGTACGA207.033838E-445.023
CGTAATC207.033838E-445.042
TTCGAAT207.033838E-445.036
TATCGCG900.045.01
CTAGCGT207.033838E-445.044
CTATCGT302.165858E-644.99999619
CGACAAT253.8912644E-544.99999643
TACTCGA253.8912644E-544.99999644
TATACGG1400.043.3928572
CGGCACG900.042.51
TCTAGCG700.041.7857131
CCTTACG603.6379788E-1241.2499961
CGAAACG603.6379788E-1241.2499961
CTAACGG2950.041.186442
TATACGC451.9288564E-840.031