##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553278_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1413853
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.724980602651055	31.0	31.0	34.0	30.0	34.0
2	31.9321400456766	33.0	31.0	34.0	30.0	34.0
3	32.101585525510785	34.0	31.0	34.0	30.0	34.0
4	35.80139873098547	37.0	35.0	37.0	35.0	37.0
5	35.559837550296955	37.0	35.0	37.0	33.0	37.0
6	34.197326737645284	37.0	35.0	37.0	32.0	37.0
7	35.03754845800801	37.0	35.0	37.0	32.0	37.0
8	35.7394799883722	37.0	35.0	37.0	33.0	37.0
9	37.428271538837485	39.0	37.0	39.0	35.0	39.0
10	36.781818194677946	39.0	37.0	39.0	32.0	39.0
11	36.80959618857123	39.0	37.0	39.0	32.0	39.0
12	36.76850139300196	39.0	37.0	39.0	32.0	39.0
13	36.76722332519717	39.0	37.0	39.0	33.0	39.0
14	37.96540234380802	40.0	37.0	41.0	33.0	41.0
15	37.94036084373694	40.0	37.0	41.0	33.0	41.0
16	37.87624597465224	40.0	37.0	41.0	33.0	41.0
17	37.85078646790013	40.0	37.0	41.0	33.0	41.0
18	37.775689551884106	39.0	37.0	41.0	33.0	41.0
19	37.804802196550845	40.0	37.0	41.0	33.0	41.0
20	37.707934983339854	39.0	36.0	41.0	33.0	41.0
21	37.67556740340049	39.0	36.0	41.0	33.0	41.0
22	37.65385086002576	39.0	36.0	41.0	33.0	41.0
23	37.518447108716394	39.0	36.0	41.0	32.0	41.0
24	37.33810870012653	39.0	36.0	41.0	32.0	41.0
25	37.41231301981182	39.0	36.0	41.0	32.0	41.0
26	37.34134170949879	39.0	36.0	41.0	32.0	41.0
27	37.29264287022767	39.0	36.0	41.0	32.0	41.0
28	37.230550842272855	39.0	36.0	41.0	32.0	41.0
29	37.038171577950465	39.0	35.0	41.0	31.0	41.0
30	37.060883274286645	39.0	35.0	41.0	31.0	41.0
31	37.01042470468995	39.0	35.0	41.0	31.0	41.0
32	36.95252264556499	39.0	35.0	40.0	31.0	41.0
33	36.88783628849675	39.0	35.0	40.0	31.0	41.0
34	36.7995795885428	39.0	35.0	40.0	31.0	41.0
35	36.69063756981808	39.0	35.0	40.0	31.0	41.0
36	36.64048737740062	39.0	35.0	40.0	31.0	41.0
37	36.59005992843669	39.0	35.0	40.0	30.0	41.0
38	36.5184393285582	39.0	35.0	40.0	30.0	41.0
39	36.47677870330225	39.0	35.0	40.0	30.0	41.0
40	36.34760120040768	38.0	35.0	40.0	30.0	41.0
41	36.253809978830894	38.0	35.0	40.0	30.0	41.0
42	36.181462287805026	38.0	35.0	40.0	30.0	41.0
43	36.111593638093915	38.0	35.0	40.0	30.0	41.0
44	35.976339124364415	38.0	35.0	40.0	30.0	41.0
45	35.80254099966545	38.0	34.0	40.0	29.0	41.0
46	35.653740523236856	38.0	34.0	40.0	29.0	41.0
47	35.555203405163056	38.0	34.0	40.0	28.0	41.0
48	35.40512132449413	38.0	34.0	40.0	28.0	41.0
49	35.25619990197001	37.0	34.0	40.0	27.0	41.0
50	35.176383966367084	37.0	34.0	40.0	27.0	41.0
51	33.99053013290632	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	7.0
10	12.0
11	26.0
12	29.0
13	27.0
14	49.0
15	98.0
16	170.0
17	293.0
18	565.0
19	961.0
20	1641.0
21	2464.0
22	3609.0
23	4787.0
24	6154.0
25	7939.0
26	9856.0
27	12592.0
28	16181.0
29	21009.0
30	27100.0
31	35328.0
32	47251.0
33	63438.0
34	94756.0
35	120956.0
36	146148.0
37	199449.0
38	289488.0
39	301431.0
40	35.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.602811607713107	29.917820310880977	20.660705179392767	27.818662902013152
2	23.61695310615743	29.54960664227469	20.66657566239206	26.16686458917582
3	21.517654239867937	31.38805802300522	20.73567761287772	26.358610124249125
4	20.946661357298108	31.999649185594258	19.635987616817307	27.417701840290327
5	18.923537312577757	33.85422671239514	16.522439037155916	30.69979693787119
6	18.792406282689928	40.1851536192235	18.28358393694394	22.738856161142635
7	88.56882575486985	5.053283474307442	3.261513042727922	3.1163777280947875
8	89.75013668323368	4.803328210217045	2.8935115602541424	2.5530235462951243
9	82.64274998886023	8.979363484039713	4.922647545395455	3.4552389817046043
10	34.87293233454963	38.89909347011323	11.845220118357425	14.382754076979715
11	24.992909446738807	29.152535659647782	26.68007211499357	19.174482778619844
12	25.533559712360475	25.757486810863643	29.27029896318783	19.438654513588048
13	24.13921390696204	25.90071245030424	28.25272500040669	21.70734864232703
14	21.3246355879996	28.105821468002684	28.14769286481692	22.421850079180793
15	20.110152894254213	30.91601460689336	27.667586375669888	21.306246123182536
16	22.75477012108048	28.540166481239567	27.163502853549836	21.541560544130117
17	22.846363801611624	28.133476393939116	27.525704581735162	21.4944552227141
18	23.003240082243344	28.560677807381673	26.811910431989748	21.624171678385235
19	22.41046275673638	30.190691677281865	26.05596197058676	21.342883595394994
20	24.088218506450104	29.510988766158857	25.056423829068507	21.34436889832253
21	24.782774446848435	28.755606134442544	26.07081499986208	20.39080441884694
22	23.500887291677422	26.313626664158157	26.05872039030932	24.126765653855102
23	21.830558056601358	29.485314244125803	26.41703203939872	22.267095659874116
24	20.647478910466646	30.301099194895087	27.392946791498122	21.658475103140145
25	22.559134506911256	29.601450787316647	25.037397805853935	22.80201689991817
26	22.907049035507935	29.14779683602185	25.020281457831896	22.92487267063832
27	21.727647782336636	28.32409026963906	27.069009295874462	22.879252652149834
28	21.493960121738258	29.585183183824626	26.993046660437827	21.927810033999293
29	21.844986713611668	30.182840790379196	25.018371782639353	22.953800713369777
30	22.726761551589874	28.559758334140817	26.49455070647373	22.218929407795578
31	23.32328749877109	28.968499554055477	25.0125720283509	22.695640918822537
32	23.412264217001344	28.812754932797112	25.37774436239128	22.39723648781026
33	21.80827851268838	28.312278574929646	26.136451243516827	23.74299166886515
34	21.73571085537181	29.34746398670866	26.37798979101788	22.53883536690165
35	22.34730201796085	27.92369503760292	26.578930058499715	23.150072885936517
36	22.443846708250433	30.180365285499978	25.594881504654303	21.780906501595286
37	20.647054538201637	31.107830870677507	25.882040070643836	22.36307452047702
38	22.75172878651458	29.948940943648317	26.1150911728447	21.184239096992403
39	21.701619616749408	27.476760313837435	26.083404710390685	24.738215359022472
40	22.167226720175297	28.58536212746304	26.324306699494223	22.92310445286745
41	20.204929366772927	27.179558270909354	28.632255262746554	23.983257099571173
42	21.398334904689527	29.149494325081886	25.682938749643704	23.769232020584884
43	20.67081938504215	28.390221614269656	26.577939856548028	24.361019144140162
44	22.495549395870718	27.512831956363215	26.440513971395895	23.551104676370173
45	23.239969077407626	28.063454970212604	26.42665114407226	22.26992480830751
46	21.581097893486806	28.11947210919381	27.395280838955678	22.904149158363705
47	21.58017842024595	29.262094432730983	26.737645285613144	22.420081861409923
48	21.00069809237594	28.943885962684945	27.44542749493759	22.60998845000152
49	21.034860059709178	28.877330245789345	27.056773228900035	23.031036465601446
50	20.881873858173375	26.878466148885348	28.59865912509999	23.64100086784128
51	21.504852343206828	26.235400709974797	28.083966296354713	24.175780650463665
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13296.0
1	10169.0
2	7042.0
3	4519.5
4	1997.0
5	1839.5
6	1682.0
7	1920.5
8	2159.0
9	2401.0
10	2643.0
11	3189.5
12	3736.0
13	4523.0
14	5310.0
15	5195.0
16	5080.0
17	4966.5
18	4853.0
19	5643.5
20	6434.0
21	6234.0
22	6034.0
23	6969.0
24	7904.0
25	9389.5
26	13320.0
27	15765.0
28	17779.5
29	19794.0
30	22800.5
31	25807.0
32	33056.5
33	40306.0
34	43371.5
35	46437.0
36	48664.5
37	50892.0
38	55136.0
39	59380.0
40	61433.0
41	63486.0
42	68338.5
43	73191.0
44	75682.5
45	78174.0
46	89565.0
47	100956.0
48	106932.5
49	112909.0
50	109373.0
51	105837.0
52	99962.5
53	94088.0
54	88591.5
55	83095.0
56	76220.0
57	69345.0
58	64637.5
59	59930.0
60	57542.0
61	55154.0
62	49519.5
63	43885.0
64	40266.5
65	36648.0
66	32957.0
67	29266.0
68	25012.5
69	20759.0
70	18991.5
71	17224.0
72	14704.0
73	12184.0
74	10345.0
75	6968.5
76	5431.0
77	4128.5
78	2826.0
79	2326.0
80	1826.0
81	1342.5
82	859.0
83	640.5
84	422.0
85	293.5
86	165.0
87	134.0
88	103.0
89	95.5
90	88.0
91	59.5
92	31.0
93	28.0
94	25.0
95	19.5
96	14.0
97	7.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1413853.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.72470756043162
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.11682560361356	16.53615776666879
2	9.003319373367814	3.9118896091916855
3	3.310611699673561	2.157662130646548
4	1.7662785026011016	1.53487535757144
5	1.1495593869824032	1.248692075277088
6	0.8083758978568525	1.0537037987904876
7	0.6268013866492393	0.9531953776399429
8	0.47997984426097173	0.83419374011769
9	0.3882172123016629	0.7590514866461663
>10	4.208789825573649	20.90778562909195
>50	1.3161207987328207	20.519915446984385
>100	0.8177771632470792	27.26133668087488
>500	0.005006798958654568	0.7099136472242703
>1k	0.0020027195834618275	0.785908489133467
>5k	0.0	0.0
>10k+	3.337865972436379E-4	0.8257187641412428
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11387	0.8053878302765562	No Hit
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT	2511	0.17759979290633468	TruSeq Adapter, Index 16 (95% over 22bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2510	0.17752906419549982	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2079	0.14704498982567496	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4145742166972097E-4	0.0	0.0	0.21628839773300337	0.0
2	1.4145742166972097E-4	0.0	0.0	0.3057602169391019	0.0
3	1.4145742166972097E-4	0.0	0.0	0.3844105433874667	0.0
4	1.4145742166972097E-4	0.0	0.0	0.7553826317163099	0.0
5	1.4145742166972097E-4	0.0	0.0	0.8268893583703539	0.0
6	1.4145742166972097E-4	0.0	0.0	1.2926379192179103	0.0
7	1.4145742166972097E-4	0.0	0.0	1.6244970304550757	0.0
8	1.4145742166972097E-4	0.0	0.0	1.9359155442609663	0.0
9	1.4145742166972097E-4	0.0	0.0	2.365521733871909	0.0
10	1.4145742166972097E-4	0.0	0.0	3.086388754700807	0.0
11	1.4145742166972097E-4	0.0	0.0	3.692533806555561	0.0
12	1.4145742166972097E-4	0.0	0.0	4.011237377577443	0.0
13	1.4145742166972097E-4	0.0	0.0	4.151492411162971	0.0
14	1.4145742166972097E-4	0.0	0.0	4.288493924050096	0.0
15	1.4145742166972097E-4	0.0	0.0	4.404630467240937	0.0
16	1.4145742166972097E-4	0.0	0.0	4.643693509862765	0.0
17	1.4145742166972097E-4	0.0	0.0	4.96345801154717	0.0
18	1.4145742166972097E-4	0.0	0.0	5.333934998900169	7.072871083486049E-5
19	1.4145742166972097E-4	0.0	0.0	5.522709928118411	7.072871083486049E-5
20	1.4145742166972097E-4	0.0	0.0	5.721669791696874	7.072871083486049E-5
21	1.4145742166972097E-4	0.0	0.0	5.948708953476776	7.072871083486049E-5
22	1.4145742166972097E-4	0.0	0.0	6.204888344120641	7.072871083486049E-5
23	1.4145742166972097E-4	0.0	0.0	6.473515987871441	7.072871083486049E-5
24	1.4145742166972097E-4	0.0	0.0	6.67169783563072	7.072871083486049E-5
25	1.4145742166972097E-4	0.0	0.0	6.844629533621954	7.072871083486049E-5
26	1.4145742166972097E-4	0.0	0.0	7.009710344710518	7.072871083486049E-5
27	1.4145742166972097E-4	0.0	0.0	7.181227468485055	7.072871083486049E-5
28	1.4145742166972097E-4	0.0	0.0	7.371416971919994	7.072871083486049E-5
29	1.4145742166972097E-4	0.0	0.0	7.5659916554266955	7.072871083486049E-5
30	1.4145742166972097E-4	0.0	0.0	7.804135224807671	7.072871083486049E-5
31	1.4145742166972097E-4	0.0	0.0	7.994890557929289	7.072871083486049E-5
32	1.4145742166972097E-4	0.0	0.0	8.189606698857661	7.072871083486049E-5
33	1.4145742166972097E-4	0.0	0.0	8.408299872759049	7.072871083486049E-5
34	1.4145742166972097E-4	0.0	0.0	8.604784231458291	7.072871083486049E-5
35	1.4145742166972097E-4	0.0	0.0	8.828357686407285	7.072871083486049E-5
36	1.4145742166972097E-4	0.0	0.0	9.045211913826968	7.072871083486049E-5
37	1.4145742166972097E-4	0.0	0.0	9.248556957477192	7.072871083486049E-5
38	2.1218613250458145E-4	0.0	0.0	9.45982361674092	7.072871083486049E-5
39	2.1218613250458145E-4	0.0	0.0	9.679153349039822	7.072871083486049E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAT	40	6.8175723E-9	45.0	34
CGCATAG	75	0.0	45.0	1
GCACGAC	20	7.033838E-4	45.0	28
ATGTCGA	20	7.033838E-4	45.0	38
CCCGTTA	35	1.2121927E-7	45.0	44
CGTACGA	20	7.033838E-4	45.0	23
CGTAATC	20	7.033838E-4	45.0	42
TTCGAAT	20	7.033838E-4	45.0	36
TATCGCG	90	0.0	45.0	1
CTAGCGT	20	7.033838E-4	45.0	44
CTATCGT	30	2.165858E-6	44.999996	19
CGACAAT	25	3.8912644E-5	44.999996	43
TACTCGA	25	3.8912644E-5	44.999996	44
TATACGG	140	0.0	43.392857	2
CGGCACG	90	0.0	42.5	1
TCTAGCG	70	0.0	41.785713	1
CCTTACG	60	3.6379788E-12	41.249996	1
CGAAACG	60	3.6379788E-12	41.249996	1
CTAACGG	295	0.0	41.18644	2
TATACGC	45	1.9288564E-8	40.0	31
>>END_MODULE