##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553276_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1305896
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.78226750062792	31.0	31.0	34.0	30.0	34.0
2	31.983180130730165	33.0	31.0	34.0	30.0	34.0
3	32.14090555450051	34.0	31.0	34.0	30.0	34.0
4	35.85122168993549	37.0	35.0	37.0	35.0	37.0
5	35.60322720951745	37.0	35.0	37.0	33.0	37.0
6	34.29975434490955	37.0	35.0	37.0	32.0	37.0
7	35.05076208212599	37.0	35.0	37.0	32.0	37.0
8	35.72059566764888	37.0	35.0	37.0	33.0	37.0
9	37.44672623241055	39.0	37.0	39.0	35.0	39.0
10	36.87342483628099	39.0	37.0	39.0	32.0	39.0
11	36.849338691595655	39.0	37.0	39.0	32.0	39.0
12	36.75725632056458	39.0	37.0	39.0	32.0	39.0
13	36.739647720798594	39.0	37.0	39.0	33.0	39.0
14	37.96072351856503	40.0	37.0	41.0	33.0	41.0
15	37.94565876608857	40.0	37.0	41.0	33.0	41.0
16	37.88575583354264	40.0	37.0	41.0	33.0	41.0
17	37.88189181986927	40.0	37.0	41.0	33.0	41.0
18	37.815653773347954	40.0	37.0	41.0	33.0	41.0
19	37.81542940632332	40.0	37.0	41.0	33.0	41.0
20	37.71724011713031	39.0	36.0	41.0	33.0	41.0
21	37.68394879837292	39.0	36.0	41.0	33.0	41.0
22	37.63655451888971	39.0	36.0	41.0	33.0	41.0
23	37.509867554537266	39.0	36.0	41.0	32.0	41.0
24	37.31123611681175	39.0	36.0	41.0	32.0	41.0
25	37.335225010261155	39.0	36.0	41.0	32.0	41.0
26	37.27045492137199	39.0	36.0	41.0	32.0	41.0
27	37.19723163253429	39.0	35.0	41.0	32.0	41.0
28	37.126167014831196	39.0	35.0	41.0	31.0	41.0
29	36.9688719469238	39.0	35.0	40.0	31.0	41.0
30	36.968324430123076	39.0	35.0	40.0	31.0	41.0
31	36.91275492075939	39.0	35.0	40.0	31.0	41.0
32	36.8283806673732	39.0	35.0	40.0	31.0	41.0
33	36.786610112903325	39.0	35.0	40.0	31.0	41.0
34	36.65677282111286	39.0	35.0	40.0	31.0	41.0
35	36.54290617323279	39.0	35.0	40.0	30.0	41.0
36	36.46745376354625	39.0	35.0	40.0	30.0	41.0
37	36.39127005519582	38.0	35.0	40.0	30.0	41.0
38	36.265597719879686	38.0	35.0	40.0	30.0	41.0
39	36.22669569399095	38.0	35.0	40.0	30.0	41.0
40	36.053489711278694	38.0	35.0	40.0	30.0	41.0
41	35.96283471271832	38.0	35.0	40.0	29.0	41.0
42	35.842166604385035	38.0	35.0	40.0	29.0	41.0
43	35.766330550059116	38.0	35.0	40.0	29.0	41.0
44	35.62021018519086	38.0	34.0	40.0	29.0	41.0
45	35.43872406378456	38.0	34.0	40.0	28.0	41.0
46	35.26641861220189	37.0	34.0	40.0	27.0	41.0
47	35.1651969222664	37.0	34.0	40.0	27.0	41.0
48	35.00860099119685	37.0	34.0	40.0	27.0	41.0
49	34.81289551388472	37.0	33.0	40.0	26.0	41.0
50	34.71386159387884	37.0	33.0	40.0	26.0	41.0
51	33.48469479958588	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	13.0
10	31.0
11	27.0
12	34.0
13	51.0
14	78.0
15	127.0
16	163.0
17	302.0
18	592.0
19	1054.0
20	1580.0
21	2514.0
22	3637.0
23	4975.0
24	6500.0
25	8215.0
26	10128.0
27	12411.0
28	15772.0
29	20250.0
30	26028.0
31	34091.0
32	44302.0
33	60164.0
34	89641.0
35	113753.0
36	137093.0
37	187699.0
38	261365.0
39	263264.0
40	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.788644731280286	30.765237047973194	20.509902779394377	25.936215441352147
2	25.35041075246421	29.77610774518032	20.190811519447184	24.682669982908287
3	21.996544900972207	30.718678975967457	19.981070468092405	27.30370565496793
4	20.823786886551456	32.33810349369322	18.764281382284654	28.07382823747067
5	19.088656370798287	34.17921488388049	16.724072973651808	30.00805577166941
6	18.77247499035145	40.47680672886661	17.922866752023133	22.8278515287588
7	86.53728934003932	6.373631590877069	3.8270275734055392	3.262051495678063
8	88.11919172736573	5.576630910884175	3.3820457371796833	2.92213162457041
9	82.46330488798496	8.177297426441308	5.365128616673916	3.99426906889982
10	39.243170972267315	31.143904261901405	12.996364182140077	16.616560583691196
11	27.74171909554819	30.349200855198273	21.878465053878713	20.030614995374822
12	26.473471088049887	25.37889694125719	27.56452274913163	20.583109221561287
13	24.931541255965254	25.798838498624697	26.80558022997237	22.464040015437675
14	22.568336222792627	27.69324662913433	26.998168307430298	22.740248840642746
15	21.6212470212023	29.603965400001226	26.74324754804364	22.031540030752833
16	23.120447570097465	27.740493883126987	26.82035935480314	22.318699191972406
17	23.23814453830933	27.480059667844912	27.054681230358312	22.227114563487444
18	24.01661388043152	27.03385261919785	26.18615877527766	22.763374725092962
19	23.27574324448501	29.524020289517694	24.738493723849373	22.46174274214792
20	24.582432291698574	28.213885332369497	25.234321875555178	21.96936050037675
21	25.356077359912277	27.653197498116235	25.805730318493968	21.18499482347752
22	24.561220801656486	26.46964229923363	24.999923424223674	23.96921347488621
23	23.035984488810747	28.823505087694578	24.965387749101	23.175122674393673
24	21.882064115366003	28.6829885381378	26.811859443631043	22.623087902865162
25	23.801895403615603	29.044043323511215	24.832528777176744	22.32153249569644
26	24.785511250513057	27.630148189442345	23.76797233470353	23.81636822534107
27	22.793469005188776	27.750601885601917	25.530363826828477	23.925565282380834
28	21.74637183971771	28.241835490728207	27.88307797864455	22.128714690909536
29	23.2975673407377	28.319713055250954	25.366644817045152	23.01607478696619
30	23.823489772539315	27.826718207269185	25.878324154450276	22.471467865741225
31	24.89011376097331	28.07206699461519	23.685040768943317	23.352778475468185
32	24.217472141732575	27.193895991717564	25.473621176571488	23.115010689978373
33	23.721261111145147	27.48970821566189	24.948158199427827	23.84087247376514
34	22.238983808817853	27.153923436475797	27.152315345172966	23.454777409533378
35	23.15689763962827	26.952605720516793	27.248494520237447	22.642002119617487
36	24.575387320276654	29.342382548074276	24.886514699486025	21.195715432163052
37	23.48280414366841	28.952918149684205	24.786889614486913	22.77738809216048
38	23.354233415218363	28.26565055716535	26.19733883862115	22.182777188995143
39	23.36035947732438	25.988746423911245	26.74791867039948	23.902975428364893
40	23.83780944271213	27.191292415322504	26.91807004539412	22.052828096571243
41	22.644605696012547	25.425761316368224	28.46689169734803	23.4627412902712
42	22.5597597358442	26.380584671367398	26.555100865612573	24.504554727175822
43	22.405765849654184	26.803282956682615	26.707486660499764	24.083464533163436
44	23.954510925831766	25.420247860472813	26.720274815146077	23.904966398549348
45	24.5916213848576	26.191901958502058	26.523551645766585	22.69292501087376
46	22.461130135937317	27.014172644682272	27.507626947321988	23.01707027205842
47	22.33592874164558	27.666291955867848	27.548365260327007	22.44941404215956
48	22.21807862188107	27.207679631456106	27.27698070903043	23.2972610376324
49	22.55991288739685	28.35095597199164	26.865156183953392	22.22397495665811
50	22.286613941692142	27.32284959904924	27.2061481159296	23.184388343329022
51	22.699893406519354	25.946476595379725	27.280579770517715	24.073050227583206
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13718.0
1	10279.5
2	6841.0
3	4328.5
4	1816.0
5	1686.0
6	1556.0
7	1739.0
8	1922.0
9	2029.5
10	2137.0
11	2385.5
12	2634.0
13	2779.5
14	2925.0
15	3319.0
16	3713.0
17	3922.0
18	4131.0
19	3870.0
20	3609.0
21	3997.0
22	4385.0
23	5407.5
24	6430.0
25	7836.5
26	12226.0
27	15209.0
28	16284.5
29	17360.0
30	22045.5
31	26731.0
32	29707.0
33	32683.0
34	36139.0
35	39595.0
36	41912.0
37	44229.0
38	45969.0
39	47709.0
40	50286.0
41	52863.0
42	56238.0
43	59613.0
44	64400.5
45	69188.0
46	76196.0
47	83204.0
48	86962.0
49	90720.0
50	90809.0
51	90898.0
52	87836.0
53	84774.0
54	81159.0
55	77544.0
56	71363.5
57	65183.0
58	63394.5
59	61606.0
60	60917.0
61	60228.0
62	54189.0
63	48150.0
64	45829.5
65	43509.0
66	39195.0
67	34881.0
68	31959.5
69	29038.0
70	24698.0
71	20358.0
72	18491.5
73	16625.0
74	13720.0
75	8963.5
76	7112.0
77	5771.0
78	4430.0
79	3526.5
80	2623.0
81	2142.5
82	1662.0
83	1338.5
84	1015.0
85	771.0
86	527.0
87	451.0
88	375.0
89	264.5
90	154.0
91	144.0
92	134.0
93	88.0
94	42.0
95	35.0
96	28.0
97	24.5
98	21.0
99	10.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1305896.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.135458144005007
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.33421976399427	15.571599451571913
2	8.638092005275038	3.4786388003255952
3	3.1595995721614925	1.9085995481122158
4	1.570676925319038	1.265051979500639
5	0.973746487550248	0.9803415821469955
6	0.6885701112561255	0.8318804792646353
7	0.5052099271980662	0.7120843340122734
8	0.4082611423406779	0.6576420114739513
9	0.3158091896182038	0.5723066447144535
>10	3.7843307458083277	18.347542425477283
>50	1.6130108555085634	23.28738620647718
>100	1.0006677532736048	29.966307662104057
>500	0.005073588452635281	0.708433566746479
>1k	0.0023416562089085915	0.7951901566175953
>5k	0.0	0.0
>10k+	3.9027603481809855E-4	0.9169951514547603
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11669	0.8935627339389968	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2469	0.18906559174696913	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2152	0.16479107065187426	No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT	1669	0.1278049706867928	TruSeq Adapter, Index 15 (95% over 22bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1532	0.11731408933023764	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.657577632522038E-5	0.0	0.0	0.15989022096706015	0.0
2	7.657577632522038E-5	0.0	0.0	0.22452017618554618	0.0
3	7.657577632522038E-5	0.0	0.0	0.2896861618383087	0.0
4	7.657577632522038E-5	0.0	0.0	0.5885614168356439	0.0
5	7.657577632522038E-5	0.0	0.0	0.641092399394745	0.0
6	7.657577632522038E-5	0.0	0.0	1.0019174574391836	0.0
7	7.657577632522038E-5	0.0	0.0	1.26648676464282	0.0
8	7.657577632522038E-5	0.0	0.0	1.493380789894448	0.0
9	7.657577632522038E-5	0.0	0.0	1.8067288666172499	0.0
10	7.657577632522038E-5	0.0	0.0	2.340155724498735	0.0
11	7.657577632522038E-5	0.0	0.0	2.86669076251095	0.0
12	7.657577632522038E-5	0.0	0.0	3.1135710653834607	0.0
13	7.657577632522038E-5	0.0	0.0	3.2207005764624443	0.0
14	7.657577632522038E-5	0.0	0.0	3.342149757714244	0.0
15	7.657577632522038E-5	0.0	0.0	3.452801754504187	0.0
16	7.657577632522038E-5	0.0	0.0	3.65879059281903	0.0
17	7.657577632522038E-5	0.0	0.0	3.9365309335506042	0.0
18	1.5315155265044076E-4	0.0	0.0	4.241685402206608	0.0
19	1.5315155265044076E-4	0.0	0.0	4.405098108884628	0.0
20	1.5315155265044076E-4	0.0	0.0	4.568893694444275	0.0
21	1.5315155265044076E-4	0.0	0.0	4.768297015995148	0.0
22	1.5315155265044076E-4	0.0	0.0	4.975511066731195	0.0
23	1.5315155265044076E-4	0.0	0.0	5.196049302547829	0.0
24	1.5315155265044076E-4	0.0	0.0	5.366124101766144	0.0
25	1.5315155265044076E-4	0.0	0.0	5.516442350692551	0.0
26	1.5315155265044076E-4	0.0	0.0	5.65826068844686	0.0
27	1.5315155265044076E-4	0.0	0.0	5.800308753530143	0.0
28	1.5315155265044076E-4	0.0	0.0	5.951469335996128	0.0
29	1.5315155265044076E-4	0.0	0.0	6.109292011002408	0.0
30	1.5315155265044076E-4	0.0	0.0	6.306780938145151	0.0
31	1.5315155265044076E-4	0.0	0.0	6.4537298529132485	0.0
32	1.5315155265044076E-4	0.0	0.0	6.611475952143203	0.0
33	1.5315155265044076E-4	0.0	0.0	6.791658753836447	0.0
34	1.5315155265044076E-4	0.0	0.0	6.953999399645913	0.0
35	1.5315155265044076E-4	0.0	0.0	7.1498036597095025	0.0
36	1.5315155265044076E-4	0.0	0.0	7.313982124150774	0.0
37	1.5315155265044076E-4	0.0	0.0	7.481223619645056	0.0
38	1.5315155265044076E-4	0.0	0.0	7.659262299601194	0.0
39	1.5315155265044076E-4	0.0	0.0	7.8438864963213	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGA	20	7.033639E-4	45.000004	1
GTATGCG	45	3.8562575E-10	45.000004	1
CACTACG	40	6.8157533E-9	45.000004	1
CGCTAAG	40	6.8157533E-9	45.000004	1
GCGATAT	20	7.033639E-4	45.000004	37
TTACGTC	20	7.033639E-4	45.000004	45
CAATACG	40	6.8157533E-9	45.000004	1
TACGTCG	20	7.033639E-4	45.000004	24
TGCGTAT	20	7.033639E-4	45.000004	26
ATTACGG	85	0.0	45.0	2
TACGGTA	25	3.8910992E-5	45.0	22
CCGCTAA	25	3.8910992E-5	45.0	24
CGTTAGA	25	3.8910992E-5	45.0	25
GTTACCG	25	3.8910992E-5	45.0	38
CGATATG	25	3.8910992E-5	45.0	1
TATCGAT	25	3.8910992E-5	45.0	13
CATTACG	60	0.0	44.999996	1
CCCGATC	185	0.0	43.783783	41
CGCATAG	155	0.0	43.548386	1
TATACGG	125	0.0	43.199997	2
>>END_MODULE