Basic Statistics
Measure | Value |
---|---|
Filename | SRR3553274_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 392006 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 41 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 17686 | 4.51166563777085 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 4106 | 1.047432947454886 | No Hit |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 3076 | 0.7846818671142789 | No Hit |
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT | 2027 | 0.5170839221848645 | TruSeq Adapter, Index 15 (95% over 24bp) |
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1470 | 0.3749942602919343 | No Hit |
AATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT | 606 | 0.154589470569328 | TruSeq Adapter, Index 15 (95% over 21bp) |
AATGATACGGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCC | 565 | 0.14413044698295432 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGT | 535 | 0.13647750289536384 | No Hit |
AAACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT | 416 | 0.10612082468125489 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTGGTT | 20 | 7.0275925E-4 | 45.000004 | 25 |
GTGCAAC | 20 | 7.0275925E-4 | 45.000004 | 45 |
AAACTCG | 20 | 7.0275925E-4 | 45.000004 | 2 |
CAGCGCG | 55 | 1.8189894E-12 | 45.000004 | 1 |
GGTACGA | 20 | 7.0275925E-4 | 45.000004 | 9 |
CGAACAT | 20 | 7.0275925E-4 | 45.000004 | 39 |
TCCGCTG | 20 | 7.0275925E-4 | 45.000004 | 11 |
TCACCTA | 20 | 7.0275925E-4 | 45.000004 | 28 |
CTCGTGA | 20 | 7.0275925E-4 | 45.000004 | 11 |
CTCGTAA | 20 | 7.0275925E-4 | 45.000004 | 33 |
CACGGAT | 20 | 7.0275925E-4 | 45.000004 | 22 |
AACCCTA | 20 | 7.0275925E-4 | 45.000004 | 11 |
CGTGCCG | 20 | 7.0275925E-4 | 45.000004 | 38 |
CTATCCA | 20 | 7.0275925E-4 | 45.000004 | 22 |
ACGCAGA | 20 | 7.0275925E-4 | 45.000004 | 41 |
GACACGA | 70 | 0.0 | 45.000004 | 25 |
TCACACG | 20 | 7.0275925E-4 | 45.000004 | 1 |
ACCGCAA | 20 | 7.0275925E-4 | 45.000004 | 33 |
CATTGCG | 40 | 6.7957444E-9 | 45.000004 | 1 |
TAGCGAG | 20 | 7.0275925E-4 | 45.000004 | 1 |