FastQCFastQC Report
Sat 18 Jun 2016
SRR3553274_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553274_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392006
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA176864.51166563777085No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41061.047432947454886No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30760.7846818671142789No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT20270.5170839221848645TruSeq Adapter, Index 15 (95% over 24bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14700.3749942602919343No Hit
AATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT6060.154589470569328TruSeq Adapter, Index 15 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCC5650.14413044698295432No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGT5350.13647750289536384No Hit
AAACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT4160.10612082468125489No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTT207.0275925E-445.00000425
GTGCAAC207.0275925E-445.00000445
AAACTCG207.0275925E-445.0000042
CAGCGCG551.8189894E-1245.0000041
GGTACGA207.0275925E-445.0000049
CGAACAT207.0275925E-445.00000439
TCCGCTG207.0275925E-445.00000411
TCACCTA207.0275925E-445.00000428
CTCGTGA207.0275925E-445.00000411
CTCGTAA207.0275925E-445.00000433
CACGGAT207.0275925E-445.00000422
AACCCTA207.0275925E-445.00000411
CGTGCCG207.0275925E-445.00000438
CTATCCA207.0275925E-445.00000422
ACGCAGA207.0275925E-445.00000441
GACACGA700.045.00000425
TCACACG207.0275925E-445.0000041
ACCGCAA207.0275925E-445.00000433
CATTGCG406.7957444E-945.0000041
TAGCGAG207.0275925E-445.0000041