##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553274_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	392006
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.591169522915465	31.0	31.0	34.0	30.0	34.0
2	31.790031275031506	31.0	31.0	34.0	30.0	34.0
3	31.98408187629781	33.0	31.0	34.0	30.0	34.0
4	35.70117294123049	37.0	35.0	37.0	33.0	37.0
5	35.452597664321466	37.0	35.0	37.0	33.0	37.0
6	34.08030489329245	37.0	35.0	37.0	32.0	37.0
7	34.9184731866349	37.0	35.0	37.0	32.0	37.0
8	35.603174441207535	37.0	35.0	37.0	32.0	37.0
9	37.26386075723331	39.0	37.0	39.0	34.0	39.0
10	36.70723917491059	38.0	37.0	39.0	32.0	39.0
11	36.74450391065443	39.0	37.0	39.0	32.0	39.0
12	36.8041790176681	39.0	37.0	39.0	32.0	39.0
13	36.77591414417126	39.0	37.0	39.0	32.0	39.0
14	37.91749871175441	40.0	37.0	41.0	33.0	41.0
15	37.91974102437207	40.0	37.0	41.0	33.0	41.0
16	37.81806145824299	40.0	37.0	41.0	33.0	41.0
17	37.739208073345814	39.0	37.0	41.0	33.0	41.0
18	37.540578460533766	39.0	36.0	40.0	33.0	41.0
19	37.39809594751101	39.0	35.0	40.0	32.0	41.0
20	37.290742998831654	39.0	35.0	40.0	32.0	41.0
21	37.24510084029326	39.0	35.0	40.0	32.0	41.0
22	37.20750702795365	39.0	35.0	40.0	32.0	41.0
23	36.981441610587595	39.0	35.0	40.0	32.0	41.0
24	36.83862236802498	39.0	35.0	40.0	31.0	41.0
25	36.90510604429524	39.0	35.0	40.0	31.0	41.0
26	36.80225557772075	39.0	35.0	40.0	31.0	41.0
27	36.803717290041476	39.0	35.0	40.0	31.0	41.0
28	36.717177798299005	39.0	35.0	40.0	31.0	41.0
29	36.52344096774029	38.0	35.0	40.0	31.0	41.0
30	36.53223930245966	38.0	35.0	40.0	31.0	41.0
31	36.43338622368025	38.0	35.0	40.0	31.0	41.0
32	36.27997020453768	38.0	35.0	40.0	30.0	41.0
33	36.258725631750536	38.0	35.0	40.0	30.0	41.0
34	36.15314306413677	38.0	35.0	40.0	30.0	41.0
35	36.01805584608399	38.0	35.0	40.0	30.0	41.0
36	35.976324341974355	38.0	35.0	40.0	30.0	41.0
37	35.89908317729831	38.0	35.0	40.0	30.0	41.0
38	35.79093177145248	38.0	35.0	40.0	29.0	41.0
39	35.711920225710834	38.0	35.0	40.0	29.0	41.0
40	35.537660137855035	38.0	35.0	40.0	28.0	41.0
41	35.44664367382132	38.0	34.0	40.0	28.0	41.0
42	35.322313944174326	38.0	34.0	40.0	27.0	41.0
43	35.27227899572966	38.0	34.0	40.0	27.0	41.0
44	35.15224766967852	38.0	34.0	40.0	26.0	41.0
45	34.942875874348864	37.0	34.0	40.0	25.0	41.0
46	34.75143237603506	37.0	33.0	40.0	24.0	41.0
47	34.658438901445386	37.0	33.0	40.0	24.0	41.0
48	34.49229603628516	37.0	33.0	40.0	24.0	41.0
49	34.3406223374132	37.0	33.0	40.0	24.0	41.0
50	34.24412126345005	37.0	33.0	40.0	23.0	41.0
51	33.13039086136437	35.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	6.0
12	9.0
13	6.0
14	9.0
15	14.0
16	49.0
17	89.0
18	205.0
19	415.0
20	741.0
21	1131.0
22	1691.0
23	2173.0
24	2722.0
25	3138.0
26	3504.0
27	4310.0
28	5211.0
29	6574.0
30	8704.0
31	11137.0
32	14463.0
33	19418.0
34	27592.0
35	43550.0
36	42000.0
37	52977.0
38	71459.0
39	68695.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.018571144319218	36.126487859879695	17.866053070616267	25.988887925184816
2	21.847369683117098	36.35582108437116	18.11094728141916	23.685861951092583
3	19.819339499905613	37.04662683734433	18.864762274046825	24.269271388703235
4	18.52344096774029	36.96065876542706	17.72268791804207	26.79321234879058
5	16.295413845706445	40.30754631306664	16.584440034081112	26.81259980714581
6	17.11376866680612	44.82865058187885	16.740304995331705	21.317275755983328
7	78.89624138406045	13.501323959327152	4.189731789819544	3.4127028667928547
8	80.17657892991433	12.760008775375887	3.314745182471697	3.7486671122380777
9	73.35015280378362	16.773212654908342	6.361892420013978	3.514742121294062
10	26.8452523685862	49.25332775518742	10.496778110539125	13.40464176568726
11	18.334923445049313	35.51833390305251	29.985255327724573	16.16148732417361
12	21.44533502038234	30.54800181629873	30.883455865471447	17.123207297847483
13	22.70016275261093	32.63291888389463	26.968464768396398	17.698453595098034
14	16.97831155645577	37.12902353535405	27.74625898583185	18.14640592235833
15	16.123732800008163	38.69558119008383	26.39908572827967	18.781600281628343
16	19.758626143477397	36.39051443090157	25.28787824676153	18.56298117885951
17	17.558149620158876	36.6172966740305	26.271281562016906	19.553272143793716
18	17.889777197287795	36.304546358984304	25.703688208853947	20.101988234873954
19	17.8604409116187	38.621602730570444	25.113391121564465	18.404565236246384
20	18.869098942363127	38.17926256230772	25.064412279403886	17.887226215925267
21	19.57776156487401	37.20708356504747	25.3021637423917	17.91299112768682
22	18.506094294475083	35.59511844206466	25.449100268873437	20.44968699458682
23	17.15790064437789	39.67617842584042	22.89378223802697	20.27213869175472
24	14.951046667653046	39.08919761432223	26.100110712591135	19.859645005433592
25	18.17089534343862	37.92084815028341	23.700912741131514	20.20734376514645
26	18.34665795931695	38.39890205762156	22.935108136099956	20.319331846961525
27	16.92754702734142	37.07366723978714	24.66186742039663	21.336918312474808
28	16.099753575200378	36.61321510385045	27.620495604659112	19.666535716290056
29	17.27601108146304	39.1713392141957	24.43023831267889	19.12241139166237
30	18.78644714621715	36.98948485482365	25.29629648525788	18.927771513701323
31	19.840767743350867	37.51626250618613	22.778477880440605	19.8644918700224
32	18.586195109258533	37.413713055412416	24.01162227108769	19.988469564241363
33	17.93237858604205	36.19842553430305	24.15065075534558	21.718545124309323
34	16.368116814538553	37.01346407963143	26.317709422814957	20.300709683015057
35	17.53748667112238	37.33922439962654	24.631255644046266	20.49203328520482
36	18.095131196971476	36.579797248001306	25.563894430187297	19.761177124839925
37	16.97678096763825	37.413457957276165	23.337397896970966	22.27236317811462
38	18.297934215292624	37.696871986653264	24.531257174635083	19.47393662341903
39	18.69002005071351	35.48006918261456	24.853191022586387	20.97671974408555
40	19.90862384759417	36.34026009805972	24.100396422503735	19.65071963184237
41	17.544629418937465	35.48670173415713	27.6493216940557	19.3193471528497
42	18.721142023336377	36.57367489273123	24.331260235812717	20.373922848119673
43	17.6237098411759	35.82470676469238	24.65523486885405	21.896348525277674
44	20.048672724397075	33.02806589695056	24.523349132411237	22.39991224624113
45	21.171614720182856	35.717565547466116	24.550134436717805	18.56068529563323
46	19.21424672071346	34.68569358632266	26.563113829890362	19.536945863073523
47	18.513492140426422	35.68797416366076	25.815931388805275	19.982602307107545
48	17.717841053453263	34.65304102488227	27.937582588021613	19.69153533364285
49	20.03362193435815	33.88774661612322	25.271041769768832	20.8075896797498
50	17.731871450947178	32.91684310954424	26.01006107049382	23.34122436901476
51	17.404070345862053	32.78648796191895	27.801615281398757	22.007826410820243
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	19703.0
1	14568.5
2	9434.0
3	5830.0
4	2226.0
5	1804.0
6	1382.0
7	1487.0
8	1592.0
9	1822.0
10	2052.0
11	2358.5
12	2665.0
13	2819.0
14	2973.0
15	3088.5
16	3204.0
17	3143.5
18	3083.0
19	3210.5
20	3338.0
21	2793.5
22	2249.0
23	2464.0
24	2679.0
25	3249.0
26	4435.0
27	5051.0
28	6234.0
29	7417.0
30	7436.0
31	7455.0
32	8408.0
33	9361.0
34	10741.0
35	12121.0
36	12729.5
37	13338.0
38	14198.0
39	15058.0
40	17192.0
41	19326.0
42	21480.5
43	23635.0
44	26416.5
45	29198.0
46	31521.5
47	33845.0
48	33026.5
49	32208.0
50	30579.0
51	28950.0
52	26872.5
53	24795.0
54	20781.0
55	16767.0
56	15668.0
57	14569.0
58	12939.0
59	11309.0
60	9630.5
61	7952.0
62	6802.5
63	5653.0
64	4849.5
65	4046.0
66	3571.0
67	3096.0
68	2369.5
69	1643.0
70	1649.5
71	1656.0
72	1360.0
73	1064.0
74	917.5
75	695.5
76	620.0
77	436.5
78	253.0
79	203.0
80	153.0
81	169.5
82	186.0
83	117.0
84	48.0
85	41.5
86	35.0
87	23.0
88	11.0
89	9.5
90	8.0
91	5.0
92	2.0
93	4.5
94	7.0
95	3.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	392006.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.701212944620917
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.76061073580456	19.42796561237389
2	11.011987236730645	5.880668323027853
3	4.061390873401167	3.253321876860735
4	2.2900341669192725	2.4458675976547624
5	1.3824092349611203	1.845600167965368
6	1.0304517137966414	1.6508586383540216
7	0.7563795809717327	1.4137376580942562
8	0.6118779953811778	1.3070307720640484
9	0.520195631208108	1.2500868889574261
>10	4.459530958236219	25.21966163531187
>50	0.7543832239053875	13.882402064410043
>100	0.3527763028566806	14.421232551652821
>500	0.0029896296852261065	0.45394805493144846
>1k	0.003986172913634808	2.841565813958346
>5k	0.0	0.0
>10k+	9.96543228408702E-4	4.706052344383117
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17686	4.51166563777085	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4106	1.047432947454886	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3076	0.7846818671142789	No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT	2027	0.5170839221848645	TruSeq Adapter, Index 15 (95% over 24bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1470	0.3749942602919343	No Hit
AATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT	606	0.154589470569328	TruSeq Adapter, Index 15 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCC	565	0.14413044698295432	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGT	535	0.13647750289536384	No Hit
AAACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT	416	0.10612082468125489	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.550981362530165E-4	0.0	0.0	0.6351943592700112	0.0
2	2.550981362530165E-4	0.0	0.0	0.8548338545838584	0.0
3	2.550981362530165E-4	0.0	0.0	1.096666887751718	0.0
4	2.550981362530165E-4	0.0	0.0	2.345372264710234	0.0
5	2.550981362530165E-4	0.0	0.0	2.5336346892649604	0.0
6	2.550981362530165E-4	0.0	0.0	3.563210767182135	0.0
7	2.550981362530165E-4	0.0	0.0	4.323403213216125	0.0
8	2.550981362530165E-4	0.0	0.0	4.8963536272404	0.0
9	2.550981362530165E-4	0.0	0.0	5.925419508885068	0.0
10	2.550981362530165E-4	0.0	0.0	7.4580491114931915	0.0
11	2.550981362530165E-4	0.0	0.0	8.924098100539277	0.0
12	2.550981362530165E-4	0.0	0.0	9.633270919322664	0.0
13	2.550981362530165E-4	0.0	0.0	9.928929659239909	0.0
14	2.550981362530165E-4	0.0	0.0	10.206221333346939	0.0
15	2.550981362530165E-4	0.0	0.0	10.450605347877328	0.0
16	2.550981362530165E-4	0.0	0.0	10.9556996576583	0.0
17	2.550981362530165E-4	0.0	0.0	11.628903639230012	0.0
18	2.550981362530165E-4	0.0	0.0	12.53195104156569	0.0
19	2.550981362530165E-4	0.0	0.0	12.888578236047408	0.0
20	2.550981362530165E-4	0.0	0.0	13.33780605398897	0.0
21	2.550981362530165E-4	0.0	0.0	13.740606011132483	0.0
22	2.550981362530165E-4	0.0	0.0	14.22835364764825	0.0
23	2.550981362530165E-4	0.0	0.0	14.676816171181054	0.0
24	2.550981362530165E-4	0.0	0.0	15.02502512716642	0.0
25	2.550981362530165E-4	0.0	0.0	15.332928577623813	0.0
26	2.550981362530165E-4	0.0	0.0	15.591598087784371	0.0
27	2.550981362530165E-4	0.0	0.0	15.932919394090908	0.0
28	2.550981362530165E-4	0.0	0.0	16.243118727774576	0.0
29	2.550981362530165E-4	0.0	0.0	16.572195323540967	0.0
30	2.550981362530165E-4	0.0	0.0	16.99540313158472	0.0
31	2.550981362530165E-4	0.0	0.0	17.29896991372581	0.0
32	2.550981362530165E-4	0.0	0.0	17.60228159773065	0.0
33	2.550981362530165E-4	0.0	0.0	17.906358576144243	0.0
34	2.550981362530165E-4	0.0	0.0	18.233904583093118	0.0
35	2.550981362530165E-4	0.0	0.0	18.554307842226905	0.0
36	2.550981362530165E-4	0.0	0.0	18.891802676489647	0.0
37	2.550981362530165E-4	0.0	0.0	19.195369458630736	0.0
38	2.550981362530165E-4	0.0	0.0	19.49613016127304	0.0
39	2.550981362530165E-4	0.0	0.0	19.810155967000505	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGGTT	20	7.0275925E-4	45.000004	25
GTGCAAC	20	7.0275925E-4	45.000004	45
AAACTCG	20	7.0275925E-4	45.000004	2
CAGCGCG	55	1.8189894E-12	45.000004	1
GGTACGA	20	7.0275925E-4	45.000004	9
CGAACAT	20	7.0275925E-4	45.000004	39
TCCGCTG	20	7.0275925E-4	45.000004	11
TCACCTA	20	7.0275925E-4	45.000004	28
CTCGTGA	20	7.0275925E-4	45.000004	11
CTCGTAA	20	7.0275925E-4	45.000004	33
CACGGAT	20	7.0275925E-4	45.000004	22
AACCCTA	20	7.0275925E-4	45.000004	11
CGTGCCG	20	7.0275925E-4	45.000004	38
CTATCCA	20	7.0275925E-4	45.000004	22
ACGCAGA	20	7.0275925E-4	45.000004	41
GACACGA	70	0.0	45.000004	25
TCACACG	20	7.0275925E-4	45.000004	1
ACCGCAA	20	7.0275925E-4	45.000004	33
CATTGCG	40	6.7957444E-9	45.000004	1
TAGCGAG	20	7.0275925E-4	45.000004	1
>>END_MODULE