FastQCFastQC Report
Sat 18 Jun 2016
SRR3553273_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553273_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388569
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA179464.618484747882615No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40981.0546389444345792No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31950.8222477861074866No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT19160.4930913171148496TruSeq Adapter, Index 15 (95% over 24bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14750.3795979607225486No Hit
AATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT5770.14849357514366818TruSeq Adapter, Index 15 (95% over 21bp)
AATGATACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGT4860.12507431112620926No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCC4860.12507431112620926No Hit
CCTATTGGGAATTAAGATAAAAATGCTTAGTTATTTATGTTAGTATCTAGC4290.1104051017965921No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTAGT302.161778E-645.00000429
CAGCGCG302.161778E-645.0000041
CGAACAT302.161778E-645.00000439
CTCAACG302.161778E-645.0000041
CGCGGAT302.161778E-645.00000423
GCGCGGA302.161778E-645.00000422
TAGCGAT302.161778E-645.00000445
ATAGCCG302.161778E-645.0000041
CGTTCGC302.161778E-645.00000418
CCATGCG302.161778E-645.0000041
TCAGGCG302.161778E-645.0000041
CCCCGGT302.161778E-645.00000426
CGTACTG302.161778E-645.0000041
GCTAGCG302.161778E-645.0000041
ATGCGCG302.161778E-645.00000428
GCGGATG302.161778E-645.00000424
GTCCCCG302.161778E-645.00000424
TGGGCGA302.161778E-645.0000046
ATACGCG302.161778E-645.0000041
CCAAACG302.161778E-645.0000041
Produced by FastQC (version 0.11.5)