##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553273_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	388569
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.568236272064937	31.0	31.0	34.0	30.0	34.0
2	31.779910389145815	31.0	31.0	34.0	30.0	34.0
3	31.9768972820786	33.0	31.0	34.0	30.0	34.0
4	35.7155820459172	37.0	35.0	37.0	33.0	37.0
5	35.488180992307676	37.0	35.0	37.0	33.0	37.0
6	32.6454838136856	37.0	35.0	37.0	26.0	37.0
7	34.20259979566049	37.0	35.0	37.0	27.0	37.0
8	35.31028723341286	37.0	35.0	37.0	32.0	37.0
9	37.14571671955303	39.0	37.0	39.0	34.0	39.0
10	36.692067560716374	38.0	37.0	39.0	32.0	39.0
11	36.76493750144762	39.0	37.0	39.0	32.0	39.0
12	36.83241329081836	39.0	37.0	39.0	32.0	39.0
13	36.825366923249156	39.0	37.0	39.0	33.0	39.0
14	37.927330795817475	40.0	37.0	41.0	33.0	41.0
15	37.985660204493925	40.0	37.0	41.0	33.0	41.0
16	37.9061530899274	40.0	37.0	41.0	33.0	41.0
17	37.84961229537096	39.0	37.0	41.0	33.0	41.0
18	37.63448448023389	39.0	37.0	41.0	33.0	41.0
19	37.49191778036848	39.0	35.0	41.0	32.0	41.0
20	37.37084533248921	39.0	35.0	40.0	32.0	41.0
21	37.30310704147783	39.0	35.0	40.0	32.0	41.0
22	37.236665817396656	39.0	35.0	40.0	32.0	41.0
23	37.01050263917091	39.0	35.0	40.0	32.0	41.0
24	36.92140649408471	39.0	35.0	40.0	31.0	41.0
25	36.95442508280382	39.0	35.0	40.0	31.0	41.0
26	36.87240361428729	39.0	35.0	40.0	31.0	41.0
27	36.833766975749484	39.0	35.0	40.0	31.0	41.0
28	36.76634523083416	39.0	35.0	40.0	31.0	41.0
29	36.54946483121402	38.0	35.0	40.0	31.0	41.0
30	36.557504587344845	38.0	35.0	40.0	31.0	41.0
31	36.133636496992814	38.0	35.0	40.0	30.0	41.0
32	36.18789455669392	38.0	35.0	40.0	30.0	41.0
33	36.204442454235924	38.0	35.0	40.0	30.0	41.0
34	36.16258631028209	38.0	35.0	40.0	30.0	41.0
35	36.0985951015135	38.0	35.0	40.0	30.0	41.0
36	36.01800195074749	38.0	35.0	40.0	30.0	41.0
37	35.918006840483926	38.0	35.0	40.0	30.0	41.0
38	35.79476232020568	38.0	35.0	40.0	29.0	41.0
39	35.70674191713698	38.0	35.0	40.0	29.0	41.0
40	35.57494807871961	38.0	35.0	40.0	28.0	41.0
41	35.54464715404471	38.0	35.0	40.0	28.0	41.0
42	35.42607876593346	38.0	34.0	40.0	28.0	41.0
43	35.389678538432044	38.0	34.0	40.0	27.0	41.0
44	35.21375611538748	38.0	34.0	40.0	27.0	41.0
45	35.001078315563	37.0	34.0	40.0	26.0	41.0
46	34.69639111715036	37.0	33.0	40.0	24.0	41.0
47	34.682468236014714	37.0	33.0	40.0	24.0	41.0
48	34.64129922870841	37.0	33.0	40.0	24.0	41.0
49	34.51538336820487	37.0	33.0	40.0	24.0	41.0
50	34.41716657787935	37.0	33.0	40.0	24.0	41.0
51	33.35367463693707	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	6.0
13	7.0
14	10.0
15	16.0
16	38.0
17	111.0
18	189.0
19	431.0
20	760.0
21	1191.0
22	1662.0
23	2134.0
24	2697.0
25	2950.0
26	3463.0
27	4169.0
28	5136.0
29	6391.0
30	8210.0
31	11099.0
32	14305.0
33	19564.0
34	27929.0
35	43967.0
36	42720.0
37	53588.0
38	69458.0
39	66360.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.93802902444611	36.22882937136004	17.840846799410144	25.992294804783704
2	21.928409111380475	36.36805818271658	18.00606842028057	23.697464285622374
3	19.83971958648271	37.14475421353736	18.921478553358607	24.094047646621323
4	18.55371890191961	36.91879691895133	17.60716886833484	26.920315310794223
5	16.245248591627227	40.441465994456586	16.459110222380065	26.85417519153612
6	16.386278884831267	47.088161948071	16.07796813435966	20.44759103273807
7	78.77468351824257	13.72935051432307	4.124621367118839	3.3713446003155165
8	79.84913876299962	13.04298593042677	3.3088074447524125	3.7990678618211953
9	73.22843561889908	16.998010649331263	6.327833666607476	3.445720065162172
10	26.814799945440836	49.50883884200747	10.414109205829595	13.2622520067221
11	18.213238832742704	35.609634324920414	30.027099434077346	16.150027408259536
12	21.405464666507108	30.681037344718696	30.730964127349324	17.18253386142487
13	22.582861731121113	32.65571880412488	26.982595111807683	17.778824352946323
14	17.010878376813384	37.30328461611709	27.61774614032514	18.068090866744388
15	16.156203917450952	38.93439775175065	26.311414446340294	18.597983884458102
16	19.778469203667818	36.64805993272752	25.144826272811265	18.428644590793397
17	17.61077183202983	36.92574549179168	25.950603367741635	19.512879308436855
18	17.923457609845357	36.57162563148373	25.59262318918905	19.912293569481868
19	17.904928082271102	38.817044077113714	24.92864845111164	18.349379389503536
20	18.74107301405928	38.33476165108385	25.005340106905077	17.918825227951793
21	19.5689826002589	37.38306452650623	25.12629674523701	17.92165612799786
22	18.687285913184017	35.7254438722595	25.34247456693663	20.244795647619856
23	17.167092588446327	39.92263922237749	22.763009915870796	20.147258273305386
24	15.001711407755122	39.28259845741683	26.01416994150331	19.701520193324736
25	18.259305297128694	38.04523778273614	23.660405230473867	20.035051689661294
26	18.223790369278042	38.611417791949435	22.833525062472816	20.331266776299707
27	16.81014182809231	37.25567402443324	24.7912211216026	21.142963025871854
28	16.226976418602614	36.620780350465424	27.6174887857755	19.53475444515646
29	17.29062277227468	39.180686055758436	24.46978528909923	19.05890588286765
30	18.525409901458943	37.16122490471448	25.30181254809313	19.01155264573345
31	19.870087423340514	37.540308156337744	22.82528971688426	19.764314703437485
32	18.506365664785402	37.63810288520186	24.00423090879612	19.851300541216617
33	17.83827325391372	36.44243364756324	24.07062838260386	21.648664715919182
34	16.277417910332527	37.01607693871616	26.351561756084507	20.354943394866805
35	17.423160365340518	37.68082374044249	24.589455154682952	20.30656073953403
36	18.268570060915824	36.59478754095154	25.429203050166123	19.707439347966513
37	16.832274319361552	37.58611726617409	23.417462535611435	22.16414587885292
38	18.23948899680623	38.01435523677905	24.486770689375632	19.259385077039084
39	18.70169776796399	35.620957925104676	24.838317004187157	20.83902730274417
40	19.789792803852084	36.60816997753295	24.01478244533146	19.58725477328351
41	17.50448440302752	35.73625276334448	27.62212116766906	19.137141665958943
42	18.663866649166557	36.78883287138191	24.28114440421135	20.266156075240175
43	17.55827150390278	36.02989430448646	24.619565636991116	21.792268554619643
44	20.10814038175974	33.21366346774962	24.471072061847448	22.2071240886432
45	21.036418242319897	36.13283612434343	24.35938018730264	18.47136544603404
46	19.09287668342045	35.04602786120354	26.404062084211553	19.457033371164453
47	18.42581369074733	35.65878904390211	25.785896455970498	20.12950080938006
48	17.54205816727531	34.926100641070185	27.861460898836498	19.670380292818006
49	19.93597018804897	34.21863298410321	25.175708818768356	20.66968800907947
50	17.660183905561176	33.254582841142756	25.81394810188152	23.27128515141455
51	17.364483528022053	33.00752247348605	27.71940118743389	21.90859281105801
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	19975.0
1	14846.5
2	9718.0
3	5949.0
4	2180.0
5	1762.5
6	1345.0
7	1496.0
8	1647.0
9	1794.0
10	1941.0
11	2236.0
12	2531.0
13	2772.5
14	3014.0
15	3162.0
16	3310.0
17	3186.5
18	3063.0
19	3111.5
20	3160.0
21	2737.5
22	2315.0
23	2474.5
24	2634.0
25	3257.0
26	4542.5
27	5205.0
28	6256.0
29	7307.0
30	7311.5
31	7316.0
32	8498.0
33	9680.0
34	10931.5
35	12183.0
36	12853.5
37	13524.0
38	14265.0
39	15006.0
40	17094.0
41	19182.0
42	21367.5
43	23553.0
44	26020.5
45	28488.0
46	30881.5
47	33275.0
48	32329.5
49	31384.0
50	29767.5
51	28151.0
52	26317.0
53	24483.0
54	20577.5
55	16672.0
56	15558.0
57	14444.0
58	12778.5
59	11113.0
60	9509.0
61	7905.0
62	6717.0
63	5529.0
64	4818.0
65	4107.0
66	3529.0
67	2951.0
68	2275.0
69	1599.0
70	1650.5
71	1702.0
72	1363.0
73	1024.0
74	911.0
75	705.5
76	613.0
77	432.5
78	252.0
79	211.5
80	171.0
81	164.0
82	157.0
83	94.0
84	31.0
85	28.0
86	25.0
87	15.5
88	6.0
89	9.0
90	12.0
91	7.0
92	2.0
93	3.5
94	5.0
95	2.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	388569.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.071318344572497
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.79188536207764	19.705723015384308
2	10.612680755040014	5.745985184180125
3	4.133775919157407	3.357202916179139
4	2.3489577471247896	2.5435753180146006
5	1.481034588530739	2.0046779412719284
6	1.0573106192989477	1.7173675418483345
7	0.8450311012286607	1.6013274166698022
8	0.6806059288090314	1.4739919812794189
9	0.5052224787199455	1.2309334700635484
>10	4.501162895578302	25.633787658677754
>50	0.6995387077562494	13.089220600556647
>100	0.33681493336412005	14.017208870912059
>500	9.964938856926629E-4	0.1556538465101953
>1k	0.0039859755427706514	2.882158918743374
>5k	0.0	0.0
>10k+	9.964938856926629E-4	4.841185319708777
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17946	4.618484747882615	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4098	1.0546389444345792	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3195	0.8222477861074866	No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT	1916	0.4930913171148496	TruSeq Adapter, Index 15 (95% over 24bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1475	0.3795979607225486	No Hit
AATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT	577	0.14849357514366818	TruSeq Adapter, Index 15 (95% over 21bp)
AATGATACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGT	486	0.12507431112620926	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCC	486	0.12507431112620926	No Hit
CCTATTGGGAATTAAGATAAAAATGCTTAGTTATTTATGTTAGTATCTAGC	429	0.1104051017965921	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5735454964240587E-4	0.0	0.0	0.5991213915675209	0.0
2	2.5735454964240587E-4	0.0	0.0	0.8134977314196449	0.0
3	2.5735454964240587E-4	0.0	0.0	1.0489771443424463	0.0
4	2.5735454964240587E-4	0.0	0.0	2.2315212999493013	0.0
5	2.5735454964240587E-4	0.0	0.0	2.403176784560786	0.0
6	2.5735454964240587E-4	0.0	0.0	3.3713446003155165	0.0
7	2.5735454964240587E-4	0.0	0.0	4.119988985225275	0.0
8	2.5735454964240587E-4	0.0	0.0	4.675360103353587	0.0
9	2.5735454964240587E-4	0.0	0.0	5.655623582941511	0.0
10	5.147090992848117E-4	0.0	0.0	7.11456652486431	0.0
11	5.147090992848117E-4	0.0	0.0	8.521523847759342	0.0
12	5.147090992848117E-4	0.0	0.0	9.205057531609572	0.0
13	5.147090992848117E-4	0.0	0.0	9.502044681896908	0.0
14	5.147090992848117E-4	0.0	0.0	9.768663995326442	0.0
15	5.147090992848117E-4	0.0	0.0	10.008518435593164	0.0
16	5.147090992848117E-4	0.0	0.0	10.485653770630185	0.0
17	5.147090992848117E-4	0.0	0.0	11.13676078122547	0.0
18	5.147090992848117E-4	0.0	0.0	11.98397195864827	0.0
19	5.147090992848117E-4	0.0	0.0	12.316474036786259	0.0
20	5.147090992848117E-4	0.0	0.0	12.757579734873342	0.0
21	5.147090992848117E-4	0.0	0.0	13.16059695961335	0.0
22	5.147090992848117E-4	0.0	0.0	13.629496949061814	0.0
23	5.147090992848117E-4	0.0	0.0	14.071117356248182	0.0
24	5.147090992848117E-4	0.0	0.0	14.429354889350412	0.0
25	5.147090992848117E-4	0.0	0.0	14.71681992130098	0.0
26	5.147090992848117E-4	0.0	0.0	14.97160092544696	0.0
27	5.147090992848117E-4	0.0	0.0	15.301272103538883	0.0
28	5.147090992848117E-4	0.0	0.0	15.595685708329794	0.0
29	5.147090992848117E-4	0.0	0.0	15.910945031641742	0.0
30	5.147090992848117E-4	0.0	0.0	16.352050729728827	0.0
31	5.147090992848117E-4	0.0	0.0	16.651611425512584	0.0
32	5.147090992848117E-4	0.0	0.0	16.970216357969885	0.0
33	5.147090992848117E-4	0.0	0.0	17.278527108441487	0.0
34	5.147090992848117E-4	0.0	0.0	17.602021777341992	0.0
35	5.147090992848117E-4	0.0	0.0	17.923200255295715	0.0
36	5.147090992848117E-4	0.0	0.0	18.247466987845144	0.0
37	7.720636489272175E-4	0.0	0.0	18.576623456837783	0.0
38	7.720636489272175E-4	0.0	0.0	18.85868404324586	0.0
39	7.720636489272175E-4	0.0	0.0	19.189641994085992	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCTAGT	30	2.161778E-6	45.000004	29
CAGCGCG	30	2.161778E-6	45.000004	1
CGAACAT	30	2.161778E-6	45.000004	39
CTCAACG	30	2.161778E-6	45.000004	1
CGCGGAT	30	2.161778E-6	45.000004	23
GCGCGGA	30	2.161778E-6	45.000004	22
TAGCGAT	30	2.161778E-6	45.000004	45
ATAGCCG	30	2.161778E-6	45.000004	1
CGTTCGC	30	2.161778E-6	45.000004	18
CCATGCG	30	2.161778E-6	45.000004	1
TCAGGCG	30	2.161778E-6	45.000004	1
CCCCGGT	30	2.161778E-6	45.000004	26
CGTACTG	30	2.161778E-6	45.000004	1
GCTAGCG	30	2.161778E-6	45.000004	1
ATGCGCG	30	2.161778E-6	45.000004	28
GCGGATG	30	2.161778E-6	45.000004	24
GTCCCCG	30	2.161778E-6	45.000004	24
TGGGCGA	30	2.161778E-6	45.000004	6
ATACGCG	30	2.161778E-6	45.000004	1
CCAAACG	30	2.161778E-6	45.000004	1
>>END_MODULE