FastQCFastQC Report
Sat 18 Jun 2016
SRR3553272_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553272_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1647092
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA147500.8955176760010977No Hit
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT33000.20035310717312696TruSeq Adapter, Index 21 (95% over 22bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28850.17515718611953673No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27270.16556452220033854No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16500.10017655358656348No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTAG302.1660762E-645.0000041
ACCGTAT302.1660762E-645.00000425
GCTCGAT302.1660762E-645.00000415
CTATGCG453.8562575E-1045.01
CGTTGAC207.034177E-445.011
TCGGCCA207.034177E-445.030
GTTACGT351.2123564E-745.033
TCGTGCA253.8915437E-545.036
CGTAGTA207.034177E-445.039
CGCTATT406.8175723E-945.034
CATACCG1100.045.01
CGTACGT253.8915437E-545.013
ACGCTAA207.034177E-445.022
CTATTCG551.8189894E-1245.026
ACGTAAG850.045.01
ATCGGCC351.2123564E-745.032
CGACTCA207.034177E-445.026
CCTATCG253.8915437E-545.018
CGTTTAG253.8915437E-545.01
CAATACG551.8189894E-1245.01