##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553272_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1647092
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.711505489675137	31.0	31.0	34.0	30.0	34.0
2	31.915399382669577	33.0	31.0	34.0	30.0	34.0
3	32.08126807731444	33.0	31.0	34.0	30.0	34.0
4	35.79375954712912	37.0	35.0	37.0	35.0	37.0
5	35.54461560131432	37.0	35.0	37.0	33.0	37.0
6	34.19351924482664	37.0	35.0	37.0	32.0	37.0
7	35.026212257724524	37.0	35.0	37.0	32.0	37.0
8	35.71909644391449	37.0	35.0	37.0	33.0	37.0
9	37.44371777654193	39.0	37.0	39.0	35.0	39.0
10	36.80112404164431	39.0	37.0	39.0	32.0	39.0
11	36.781942963720304	39.0	37.0	39.0	32.0	39.0
12	36.712458684760776	39.0	35.0	39.0	32.0	39.0
13	36.69373174054637	39.0	35.0	39.0	32.0	39.0
14	37.87106791848907	40.0	37.0	41.0	33.0	41.0
15	37.873521333356	40.0	37.0	41.0	33.0	41.0
16	37.803009789374244	40.0	37.0	41.0	33.0	41.0
17	37.77382319870414	39.0	37.0	41.0	33.0	41.0
18	37.725969162621155	39.0	37.0	41.0	33.0	41.0
19	37.75171210837039	39.0	36.0	41.0	33.0	41.0
20	37.64898196336331	39.0	36.0	41.0	33.0	41.0
21	37.601269388716595	39.0	36.0	41.0	32.0	41.0
22	37.59800120454717	39.0	36.0	41.0	32.0	41.0
23	37.444565330898335	39.0	36.0	41.0	32.0	41.0
24	37.249375869714626	39.0	36.0	41.0	32.0	41.0
25	37.33487261185167	39.0	36.0	41.0	32.0	41.0
26	37.237073581803564	39.0	36.0	41.0	32.0	41.0
27	37.21145509783303	39.0	36.0	41.0	32.0	41.0
28	37.10499899216316	39.0	35.0	41.0	31.0	41.0
29	36.94388291607269	39.0	35.0	40.0	31.0	41.0
30	36.96886573427592	39.0	35.0	40.0	31.0	41.0
31	36.902172434812385	39.0	35.0	40.0	31.0	41.0
32	36.82154427317964	39.0	35.0	40.0	31.0	41.0
33	36.76128291558699	39.0	35.0	40.0	31.0	41.0
34	36.64879132434618	39.0	35.0	40.0	30.0	41.0
35	36.53937667112705	39.0	35.0	40.0	30.0	41.0
36	36.449951186697525	39.0	35.0	40.0	30.0	41.0
37	36.39159439788427	38.0	35.0	40.0	30.0	41.0
38	36.27227319421137	38.0	35.0	40.0	30.0	41.0
39	36.24370405538974	38.0	35.0	40.0	30.0	41.0
40	36.095484040964315	38.0	35.0	40.0	30.0	41.0
41	36.00446423150619	38.0	35.0	40.0	30.0	41.0
42	35.90472116918788	38.0	35.0	40.0	29.0	41.0
43	35.825196163905844	38.0	35.0	40.0	29.0	41.0
44	35.69196438328885	38.0	34.0	40.0	29.0	41.0
45	35.510921065732816	38.0	34.0	40.0	28.0	41.0
46	35.35520238092347	38.0	34.0	40.0	28.0	41.0
47	35.25223120505716	37.0	34.0	40.0	27.0	41.0
48	35.1184214360825	37.0	34.0	40.0	27.0	41.0
49	34.95131540921819	37.0	34.0	40.0	27.0	41.0
50	34.8614102915927	37.0	34.0	40.0	26.0	41.0
51	33.62059010668499	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	6.0
10	9.0
11	17.0
12	19.0
13	29.0
14	69.0
15	130.0
16	232.0
17	420.0
18	708.0
19	1216.0
20	2144.0
21	3063.0
22	4314.0
23	6036.0
24	8010.0
25	10127.0
26	12894.0
27	16018.0
28	20607.0
29	26162.0
30	33483.0
31	43294.0
32	57352.0
33	77148.0
34	112603.0
35	143836.0
36	173205.0
37	233518.0
38	329088.0
39	331294.0
40	38.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.734912196768608	30.9156379850063	19.8645248717133	27.48492494651179
2	24.64640712237082	30.352342188535918	19.15418203719039	25.847068651902873
3	22.056691429501203	30.734409492608794	19.74874506099234	27.460154016897658
4	20.513183234451994	31.78407763500764	19.776369504557124	27.92636962598325
5	19.288661471247508	34.56595017157512	16.82298256563689	29.322405791540483
6	19.168024615504173	41.28822190867298	17.106694708006597	22.437058767816247
7	87.31054488759582	5.720688340420572	3.564160350484369	3.404606421499224
8	88.43215800938867	5.441347538571009	3.1154908165421236	3.011003635498199
9	82.15260592608064	8.600369621126202	5.3354032440203705	3.911621208772795
10	35.80249312120999	36.26069460600865	12.412057128563553	15.524755144217808
11	26.886172721378042	30.75371624657275	24.023673237439073	18.336437794610134
12	26.708829864998435	26.82175616176874	28.153011489340003	18.316402483892823
13	25.058709531708008	27.44898281334619	27.06205846425093	20.430249190694873
14	22.17271409247328	30.22332693012898	26.005529745757975	21.598429231639763
15	21.5207165112817	30.91357374087179	26.515883751484438	21.049825996362074
16	23.491766094425813	29.60630007309853	25.669482943272143	21.232450889203516
17	21.703462830248704	29.692755474496874	27.16618136691818	21.43760032833624
18	24.005216466354035	28.1904714490751	25.93364547942677	21.870666605144095
19	22.51040014765417	30.643097046188068	25.268473163611986	21.578029642545772
20	25.469190549161798	29.96122863810886	24.518606125219478	20.050974687509864
21	25.133568738115418	29.131281069909875	25.901042564714054	19.83410762726065
22	24.688784840191076	27.160352912891327	24.83692471337363	23.313937533543967
23	22.837097138471925	30.889652794136573	24.48818888076683	21.78506118662467
24	20.855422769341363	31.15241892984727	26.577325371017523	21.414832929793842
25	23.84092691847207	30.025159493215924	23.94893545715722	22.184978131154786
26	23.878751156583846	30.461747127665	23.483751970138886	22.175749745612265
27	22.4473799884888	28.753767245545482	26.1064348560979	22.692417909867814
28	21.65392097102044	30.749830610554845	26.458752759408704	21.13749565901601
29	22.60650892603449	30.979204561736683	24.749315763782473	21.66497074844635
30	23.166101225675312	28.99753019260612	26.363251111656176	21.473117470062387
31	23.889375942570297	29.769739638101576	24.17302737187722	22.1678570474509
32	22.78597673961139	30.017873925682352	26.080146099914277	21.116003234791986
33	22.89562453099159	29.245118062621884	24.812518062136178	23.04673934425035
34	21.415500773484418	30.469882678077486	26.497184128148277	21.61743242028982
35	23.09731332554587	29.3007919411909	26.471259650341327	21.1306350829219
36	23.219407294795918	32.056497147700306	24.79594339599731	19.92815216150646
37	22.346110599772203	31.23948146187341	24.21060875773788	22.203799180616503
38	21.956454162851863	31.689183117882912	25.9266634772071	20.427699242058125
39	22.924706088063083	28.408127779140447	25.311883003499503	23.355283129296968
40	24.339502590019258	28.59597399538095	26.022165124959624	21.042358289640166
41	21.87479509341312	27.709016861231795	27.70731689547396	22.708871149881123
42	23.63942026310613	29.512376964978277	25.234413135392558	21.613789636523038
43	22.66631129287253	28.740653223985056	26.000126283170584	22.592909199971828
44	23.686412173697644	27.927766026427182	25.974262518426418	22.41155928144876
45	24.25171150123976	28.346868298795698	25.698807352594756	21.70261284736979
46	21.726108802665546	29.59810380962326	25.792001903961648	22.88378548374954
47	22.12869712195797	29.785342895235967	26.460756290480436	21.625203692325627
48	21.96386115651099	29.10049954708055	27.2712756785899	21.664363617818555
49	22.37537429603204	29.682373540761535	26.352808464858064	21.58944369834836
50	21.88420561814398	28.935724294696353	26.78733185517263	22.392738231987042
51	22.128454269706854	27.377705677642776	27.662996359644755	22.830843693005612
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17492.0
1	13026.0
2	8560.0
3	5566.5
4	2573.0
5	2291.0
6	2009.0
7	2345.5
8	2682.0
9	3145.0
10	3608.0
11	4022.0
12	4436.0
13	4981.0
14	5526.0
15	5946.0
16	6366.0
17	5825.0
18	5284.0
19	5568.5
20	5853.0
21	6658.0
22	7463.0
23	9538.5
24	11614.0
25	12582.5
26	13995.0
27	14439.0
28	18802.0
29	23165.0
30	28641.0
31	34117.0
32	38698.0
33	43279.0
34	49711.0
35	56143.0
36	59080.0
37	62017.0
38	62824.0
39	63631.0
40	66105.5
41	68580.0
42	79236.0
43	89892.0
44	96986.5
45	104081.0
46	113468.5
47	122856.0
48	121898.5
49	120941.0
50	119147.5
51	117354.0
52	110376.0
53	103398.0
54	96370.0
55	89342.0
56	86503.0
57	83664.0
58	77046.0
59	70428.0
60	65908.0
61	61388.0
62	58033.0
63	54678.0
64	49183.5
65	43689.0
66	38254.5
67	32820.0
68	28610.0
69	24400.0
70	21611.0
71	18822.0
72	18417.5
73	18013.0
74	14968.5
75	10130.0
76	8336.0
77	6267.0
78	4198.0
79	3190.0
80	2182.0
81	1817.5
82	1453.0
83	963.0
84	473.0
85	308.5
86	144.0
87	107.5
88	71.0
89	78.5
90	86.0
91	54.5
92	23.0
93	17.5
94	12.0
95	14.0
96	16.0
97	10.5
98	5.0
99	10.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1647092.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.21683074156173
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.46563921448133	15.458928855444942
2	9.368323414079121	3.7879561758929468
3	3.434505721643874	2.083044625661986
4	1.7944727256375963	1.451142054582569
5	1.100398842197619	1.112328857045988
6	0.7783055786087613	0.9440923288767956
7	0.6210159149727957	0.8788481548574771
8	0.46409264928170796	0.7505986031145009
9	0.35703009541200426	0.6496215307729314
>10	3.48227079480729	15.674494066522456
>50	1.005875506013387	14.603122137284888
>100	1.115449938155857	39.39344476535095
>500	0.008310471393530012	0.9823066142631981
>1k	0.004001338078366302	1.3122312100330875
>5k	0.0	0.0
>10k+	3.077952367974079E-4	0.9178400202953358
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14750	0.8955176760010977	No Hit
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT	3300	0.20035310717312696	TruSeq Adapter, Index 21 (95% over 22bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2885	0.17515718611953673	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2727	0.16556452220033854	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1650	0.10017655358656348	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2478914353296598	0.0
2	0.0	0.0	0.0	0.34794656279066377	0.0
3	0.0	0.0	0.0	0.4463624375566149	0.0
4	0.0	0.0	0.0	0.8737216864631727	0.0
5	0.0	0.0	0.0	0.9451202482921416	0.0
6	0.0	0.0	0.0	1.429003358646633	0.0
7	0.0	0.0	0.0	1.7742178336122088	0.0
8	0.0	0.0	0.0	2.093811396084736	0.0
9	0.0	0.0	0.0	2.533981101237818	0.0
10	6.071306277973544E-5	0.0	0.0	3.2805089211774447	0.0
11	6.071306277973544E-5	0.0	0.0	3.868636360324742	0.0
12	6.071306277973544E-5	0.0	0.0	4.167283916138261	0.0
13	6.071306277973544E-5	0.0	0.0	4.303949020455445	0.0
14	6.071306277973544E-5	0.0	0.0	4.442010525216563	0.0
15	6.071306277973544E-5	0.0	0.0	4.567200860668378	0.0
16	6.071306277973544E-5	0.0	0.0	4.81922078426706	0.0
17	6.071306277973544E-5	0.0	0.0	5.162067449784225	0.0
18	6.071306277973544E-5	0.0	0.0	5.5517238867045675	0.0
19	6.071306277973544E-5	0.0	0.0	5.739570102945069	0.0
20	6.071306277973544E-5	0.0	0.0	5.947390916840104	0.0
21	6.071306277973544E-5	0.0	0.0	6.180954069353746	0.0
22	6.071306277973544E-5	0.0	0.0	6.453312868983639	0.0
23	6.071306277973544E-5	0.0	0.0	6.73313937533544	0.0
24	6.071306277973544E-5	0.0	0.0	6.947638626136245	0.0
25	6.071306277973544E-5	0.0	0.0	7.124860056390292	0.0
26	6.071306277973544E-5	0.0	0.0	7.2957066150524685	0.0
27	6.071306277973544E-5	0.0	0.0	7.476813681324419	0.0
28	6.071306277973544E-5	0.0	0.0	7.66253494036763	0.0
29	6.071306277973544E-5	0.0	0.0	7.8558453322583075	0.0
30	6.071306277973544E-5	0.0	0.0	8.117579345901747	0.0
31	6.071306277973544E-5	0.0	0.0	8.30931119816015	0.0
32	6.071306277973544E-5	0.0	0.0	8.508814322454363	0.0
33	6.071306277973544E-5	0.0	0.0	8.717302980039973	0.0
34	1.2142612555947087E-4	0.0	0.0	8.916988243522523	0.0
35	1.2142612555947087E-4	0.0	0.0	9.146605046955482	0.0
36	1.2142612555947087E-4	0.0	0.0	9.344893909994099	0.0
37	1.2142612555947087E-4	0.0	0.0	9.545307730230006	0.0
38	1.8213918833920632E-4	0.0	0.0	9.76235692966756	0.0
39	1.8213918833920632E-4	0.0	0.0	9.986084566010884	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCTAG	30	2.1660762E-6	45.000004	1
ACCGTAT	30	2.1660762E-6	45.000004	25
GCTCGAT	30	2.1660762E-6	45.000004	15
CTATGCG	45	3.8562575E-10	45.0	1
CGTTGAC	20	7.034177E-4	45.0	11
TCGGCCA	20	7.034177E-4	45.0	30
GTTACGT	35	1.2123564E-7	45.0	33
TCGTGCA	25	3.8915437E-5	45.0	36
CGTAGTA	20	7.034177E-4	45.0	39
CGCTATT	40	6.8175723E-9	45.0	34
CATACCG	110	0.0	45.0	1
CGTACGT	25	3.8915437E-5	45.0	13
ACGCTAA	20	7.034177E-4	45.0	22
CTATTCG	55	1.8189894E-12	45.0	26
ACGTAAG	85	0.0	45.0	1
ATCGGCC	35	1.2123564E-7	45.0	32
CGACTCA	20	7.034177E-4	45.0	26
CCTATCG	25	3.8915437E-5	45.0	18
CGTTTAG	25	3.8915437E-5	45.0	1
CAATACG	55	1.8189894E-12	45.0	1
>>END_MODULE