##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553271_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1640785
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.69787814978806	31.0	31.0	34.0	30.0	34.0
2	31.90739920221114	33.0	31.0	34.0	30.0	34.0
3	32.06832644130706	33.0	31.0	34.0	30.0	34.0
4	35.794967652678444	37.0	35.0	37.0	35.0	37.0
5	35.56431403261244	37.0	35.0	37.0	33.0	37.0
6	32.76028120686135	37.0	35.0	37.0	26.0	37.0
7	34.3062497524051	37.0	35.0	37.0	28.0	37.0
8	35.434692540460816	37.0	35.0	37.0	32.0	37.0
9	37.32451174285479	39.0	37.0	39.0	34.0	39.0
10	36.762170546415284	39.0	37.0	39.0	32.0	39.0
11	36.791664355780924	39.0	37.0	39.0	32.0	39.0
12	36.733817044890095	39.0	35.0	39.0	32.0	39.0
13	36.72994755559077	39.0	37.0	39.0	32.0	39.0
14	37.890036781174864	40.0	37.0	41.0	33.0	41.0
15	37.92239568255439	40.0	37.0	41.0	33.0	41.0
16	37.877504365288566	40.0	37.0	41.0	33.0	41.0
17	37.859384989501976	40.0	37.0	41.0	33.0	41.0
18	37.80379940089652	39.0	37.0	41.0	33.0	41.0
19	37.82852963672876	40.0	37.0	41.0	33.0	41.0
20	37.71310196034215	39.0	36.0	41.0	33.0	41.0
21	37.653702953159616	39.0	36.0	41.0	33.0	41.0
22	37.62379714587834	39.0	36.0	41.0	33.0	41.0
23	37.47685041001716	39.0	36.0	41.0	32.0	41.0
24	37.34270486383042	39.0	36.0	41.0	32.0	41.0
25	37.389657389603144	39.0	36.0	41.0	32.0	41.0
26	37.31190741017257	39.0	36.0	41.0	32.0	41.0
27	37.257766252129315	39.0	36.0	41.0	32.0	41.0
28	37.18190622171704	39.0	35.0	41.0	32.0	41.0
29	36.98894797307386	39.0	35.0	40.0	31.0	41.0
30	37.018986643588285	39.0	35.0	40.0	31.0	41.0
31	36.65207202649951	39.0	35.0	40.0	30.0	41.0
32	36.75427127868672	39.0	35.0	40.0	31.0	41.0
33	36.743190606935094	39.0	35.0	40.0	31.0	41.0
34	36.668126536992965	39.0	35.0	40.0	31.0	41.0
35	36.63698534542917	39.0	35.0	40.0	31.0	41.0
36	36.52290824209144	39.0	35.0	40.0	30.0	41.0
37	36.44587925901322	39.0	35.0	40.0	30.0	41.0
38	36.32057643140326	38.0	35.0	40.0	30.0	41.0
39	36.271238462077605	38.0	35.0	40.0	30.0	41.0
40	36.14864896985284	38.0	35.0	40.0	30.0	41.0
41	36.11075978875965	38.0	35.0	40.0	30.0	41.0
42	36.00294980756163	38.0	35.0	40.0	30.0	41.0
43	35.943430126433384	38.0	35.0	40.0	29.0	41.0
44	35.76781967168154	38.0	35.0	40.0	29.0	41.0
45	35.57954454727463	38.0	34.0	40.0	28.0	41.0
46	35.33448562730644	38.0	34.0	40.0	27.0	41.0
47	35.29704196466935	37.0	34.0	40.0	28.0	41.0
48	35.26097873883538	37.0	34.0	40.0	27.0	41.0
49	35.14358493038393	37.0	34.0	40.0	27.0	41.0
50	35.021785303985595	37.0	34.0	40.0	27.0	41.0
51	33.853638959400534	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	12.0
10	9.0
11	15.0
12	34.0
13	34.0
14	56.0
15	110.0
16	238.0
17	409.0
18	686.0
19	1234.0
20	1933.0
21	3092.0
22	4309.0
23	5993.0
24	7944.0
25	9843.0
26	12629.0
27	15637.0
28	20056.0
29	25492.0
30	32941.0
31	42882.0
32	56928.0
33	77962.0
34	113814.0
35	145277.0
36	175711.0
37	238897.0
38	323594.0
39	322961.0
40	53.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.71673924371566	30.86102079187706	19.885908269517337	27.53633169488995
2	24.71658383030074	30.20011762662384	19.120116285802222	25.963182257273193
3	21.98252665644798	30.68695776716633	19.77803307563148	27.55248250075421
4	20.47593072827945	31.75449556157571	19.75895684078048	28.01061686936436
5	19.29393552476406	34.522256115213146	16.867657858890713	29.316150501132082
6	18.416367775180785	43.786724037579575	16.412875544327868	21.384032642911777
7	87.37293429669334	5.720249758499742	3.550251861151827	3.3565640836550794
8	88.27244276367713	5.514189854246595	3.064021184981579	3.149346197094683
9	82.14696014407737	8.629832671556603	5.328607952900594	3.8945992314654267
10	35.85113223243752	36.18237611874804	12.464338715919514	15.502152932894925
11	26.88176695910799	30.803548301575162	23.94292975618378	18.371754983133073
12	26.77346514016157	26.730254116170006	28.173343856751494	18.32293688691693
13	25.075009827613005	27.4439368960589	27.076734611786428	20.40431866454167
14	22.157869556340408	30.26130784959638	26.021812729882342	21.559009864180865
15	21.58917835060657	30.87656213336909	26.590503935616184	20.94375558040816
16	23.49381546028273	29.58510712860003	25.680512681429924	21.240564729687314
17	21.670541844300136	29.694201251230357	27.239949170671355	21.395307733798152
18	24.007228247454723	28.197661485203728	25.99907970879792	21.796030558543624
19	22.486858424473652	30.636250331396255	25.361884707624704	21.515006536505393
20	25.429413360068505	29.92994207041142	24.636134533165528	20.00451003635455
21	25.148389338030274	29.11435684748459	25.96562011476214	19.771633699722997
22	24.629796103694268	27.186621038100668	24.888635622583095	23.294947235621972
23	22.861679013399076	30.9289151229442	24.519361159445022	21.690044704211704
24	20.787915540427296	31.19037533863364	26.6325569773005	21.38915214363856
25	23.84797520698934	30.032697763570486	23.92458487857946	22.194742150860716
26	23.861017744555195	30.456153609400378	23.563172505843237	22.119656140201187
27	22.456689938047948	28.846558202323884	26.03643987481602	22.660311984812147
28	21.69638313368296	30.765822456933723	26.39334221119769	21.144452198185625
29	22.570476936344495	31.037155995453396	24.67660296748203	21.71576410072008
30	23.152698251141985	29.01714728011287	26.328434255554505	21.501720213190637
31	23.885761998068	29.827430163001246	24.111080976483816	22.175726862446936
32	22.775805483350958	30.02130077980966	26.11853472575627	21.08435901108311
33	22.84875836870766	29.315845768945962	24.82896906054114	23.006426801805233
34	21.439676740096967	30.476387826558625	26.501887815893006	21.5820476174514
35	23.032511876936955	29.289638800939795	26.50322863751192	21.174620684611327
36	23.195848328696325	32.11743159524252	24.788074001164077	19.898646074897076
37	22.302739237621015	31.24864013261945	24.304281182482775	22.144339447276764
38	21.8939105367248	31.76284522347535	25.972568008605634	20.370676231194214
39	22.91189887767136	28.442788055717234	25.362555118434166	23.28275794817724
40	24.272162410065913	28.64317994130858	26.00822167438147	21.076435974244035
41	21.847042726499815	27.793220927787615	27.665355302492404	22.694381043220165
42	23.527884518690747	29.629476134898848	25.21018902537505	21.632450321035357
43	22.57328047245678	28.898545513275657	26.031381320526453	22.496792693741106
44	23.600837404047454	28.006289672321483	25.99133951127052	22.401533412360546
45	24.20438997187322	28.41194915848207	25.75127149504658	21.632389374598134
46	21.762449071633394	29.608205828307792	25.80027243057439	22.829072669484425
47	22.113744335790493	29.811523142885875	26.547719536685182	21.52701298463845
48	21.871299408514826	29.13593188626176	27.297116928787133	21.69565177643628
49	22.333334349107286	29.75392875970953	26.38517538860972	21.527561502573462
50	21.909756610402948	28.988441508180536	26.766151567694727	22.335650313721786
51	22.054809130995228	27.485989937743216	27.604226025957086	22.854974905304474
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17475.0
1	13075.5
2	8676.0
3	5689.5
4	2703.0
5	2348.0
6	1993.0
7	2352.0
8	2711.0
9	3146.0
10	3581.0
11	4055.0
12	4529.0
13	5053.0
14	5577.0
15	5930.5
16	6284.0
17	5775.5
18	5267.0
19	5567.5
20	5868.0
21	6728.5
22	7589.0
23	9688.5
24	11788.0
25	12611.5
26	14037.5
27	14640.0
28	18889.0
29	23138.0
30	28898.5
31	34659.0
32	39344.5
33	44030.0
34	50041.5
35	56053.0
36	59130.5
37	62208.0
38	63089.0
39	63970.0
40	66433.0
41	68896.0
42	79330.5
43	89765.0
44	96181.5
45	102598.0
46	112015.5
47	121433.0
48	120823.5
49	120214.0
50	118115.0
51	116016.0
52	109786.0
53	103556.0
54	96148.5
55	88741.0
56	85944.5
57	83148.0
58	76492.0
59	69836.0
60	65648.5
61	61461.0
62	58019.0
63	54577.0
64	49071.5
65	43566.0
66	37794.0
67	32022.0
68	27983.5
69	23945.0
70	21501.5
71	19058.0
72	18347.0
73	17636.0
74	14701.5
75	9880.5
76	7994.0
77	6087.5
78	4181.0
79	3132.0
80	2083.0
81	1702.0
82	1321.0
83	877.0
84	433.0
85	285.5
86	138.0
87	109.0
88	80.0
89	77.0
90	74.0
91	50.5
92	27.0
93	22.5
94	18.0
95	14.5
96	11.0
97	8.0
98	5.0
99	8.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1640785.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.769389280935314
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.6315508620693	15.70821121775176
2	9.224328571087042	3.831673418963212
3	3.5957808952919272	2.2404651954980443
4	1.9065169742998636	1.5838877283977926
5	1.2296687137580726	1.2769734101314216
6	0.900262510664759	1.1218741523417137
7	0.6953761522556454	1.0109776602013512
8	0.5313598174060663	0.8828815116762642
9	0.4550751538605191	0.850646972034959
>10	3.8348373174684447	17.272453967394007
>50	0.9622205690241343	14.435641012593345
>100	1.0229499753649816	36.87939531775427
>500	0.006813741509721947	0.9103250921312407
>1k	0.0029624963085747597	1.093374578382707
>5k	0.0	0.0
>10k+	2.96249630857476E-4	0.9012187647479745
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14647	0.892682466014743	No Hit
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT	3077	0.18753218733715873	TruSeq Adapter, Index 21 (95% over 22bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2982	0.18174227580091237	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2702	0.1646772733782915	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1688	0.10287758603351445	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.0946437223645996E-5	0.0	0.0	0.22745210371864688	0.0
2	6.0946437223645996E-5	0.0	0.0	0.32484451040203316	0.0
3	6.0946437223645996E-5	0.0	0.0	0.417665934293646	0.0
4	6.0946437223645996E-5	0.0	0.0	0.8188763305369077	0.0
5	6.0946437223645996E-5	0.0	0.0	0.8882943225346405	0.0
6	6.0946437223645996E-5	0.0	0.0	1.32808381354047	0.0
7	6.0946437223645996E-5	0.0	0.0	1.6564632173014746	0.0
8	6.0946437223645996E-5	0.0	0.0	1.974725512483354	0.0
9	6.0946437223645996E-5	0.0	0.0	2.406531020212886	0.0
10	6.0946437223645996E-5	0.0	0.0	3.1244800507074357	0.0
11	6.0946437223645996E-5	0.0	0.0	3.7082859728727406	0.0
12	6.0946437223645996E-5	0.0	0.0	4.007411086766395	0.0
13	6.0946437223645996E-5	0.0	0.0	4.137714569550551	0.0
14	1.2189287444729199E-4	0.0	0.0	4.273259445935939	0.0
15	1.2189287444729199E-4	0.0	0.0	4.393080141517627	0.0
16	1.2189287444729199E-4	0.0	0.0	4.638084819156684	0.0
17	1.2189287444729199E-4	0.0	0.0	4.961344722190902	0.0
18	1.2189287444729199E-4	0.0	0.0	5.342016169089796	0.0
19	1.82839311670938E-4	0.0	0.0	5.528756052743047	0.0
20	1.82839311670938E-4	0.0	0.0	5.721163955058097	0.0
21	1.82839311670938E-4	0.0	0.0	5.94404507598497	0.0
22	1.82839311670938E-4	0.0	0.0	6.196729004714207	0.0
23	1.82839311670938E-4	0.0	0.0	6.462638310320974	0.0
24	1.82839311670938E-4	0.0	0.0	6.669490518258029	0.0
25	1.82839311670938E-4	0.0	0.0	6.846661811267168	0.0
26	1.82839311670938E-4	0.0	0.0	7.011217191771012	0.0
27	1.82839311670938E-4	0.0	0.0	7.18686482384956	0.0
28	1.82839311670938E-4	0.0	0.0	7.368302367464354	0.0
29	1.82839311670938E-4	0.0	0.0	7.56064932334218	0.0
30	1.82839311670938E-4	0.0	0.0	7.814186502192548	0.0
31	1.82839311670938E-4	0.0	0.0	8.005314529325902	0.0
32	1.82839311670938E-4	0.0	0.0	8.206315879289486	0.0
33	1.82839311670938E-4	0.0	0.0	8.412619569291529	0.0
34	1.82839311670938E-4	0.0	0.0	8.603442864238763	0.0
35	1.82839311670938E-4	0.0	0.0	8.823581395490573	0.0
36	2.4378574889458399E-4	0.0	0.0	9.013612386753902	0.0
37	2.4378574889458399E-4	0.0	0.0	9.21199303991687	0.0
38	2.4378574889458399E-4	0.0	0.0	9.417870104858345	0.0
39	3.0473218611823E-4	0.0	0.0	9.632035885262237	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCCG	25	3.8915372E-5	45.0	1
TTGTCGA	25	3.8915372E-5	45.0	12
CGCGCAT	20	7.0341694E-4	45.0	41
CGTCTAC	25	3.8915372E-5	45.0	13
TTATCGT	45	3.8562575E-10	45.0	21
CGCCTAT	20	7.0341694E-4	45.0	41
CCGTAAA	25	3.8915372E-5	45.0	30
CGCTATT	25	3.8915372E-5	45.0	34
ACGTCGT	25	3.8915372E-5	45.0	18
ACGTAGC	20	7.0341694E-4	45.0	1
CAGTCGA	20	7.0341694E-4	45.0	28
ACGTAAG	100	0.0	45.0	1
ATCGGGT	55	1.8189894E-12	45.0	4
GGCGAAT	35	1.2123564E-7	45.0	8
CGTTTAG	25	3.8915372E-5	45.0	1
TTCGACG	20	7.0341694E-4	45.0	1
TACGTCG	90	0.0	45.0	1
TATCCGT	20	7.0341694E-4	45.0	30
ACGCCTA	30	2.1660726E-6	44.999996	14
TCCGCAC	30	2.1660726E-6	44.999996	43
>>END_MODULE