##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553266_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	952881
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.698714739825853	31.0	31.0	34.0	30.0	34.0
2	31.89442123413102	33.0	31.0	34.0	30.0	34.0
3	32.06256185189966	33.0	31.0	34.0	30.0	34.0
4	35.77083392364839	37.0	35.0	37.0	35.0	37.0
5	35.527666098914764	37.0	35.0	37.0	33.0	37.0
6	34.19025250792072	37.0	35.0	37.0	32.0	37.0
7	34.973022864345076	37.0	35.0	37.0	32.0	37.0
8	35.64789622208859	37.0	35.0	37.0	33.0	37.0
9	37.36093908893135	39.0	37.0	39.0	35.0	39.0
10	36.766729528660974	39.0	37.0	39.0	32.0	39.0
11	36.751720309251624	39.0	37.0	39.0	32.0	39.0
12	36.635323823226614	39.0	35.0	39.0	32.0	39.0
13	36.60057971562031	39.0	35.0	39.0	32.0	39.0
14	37.75592230299481	40.0	37.0	41.0	33.0	41.0
15	37.75167623239418	40.0	37.0	41.0	33.0	41.0
16	37.69960467256667	40.0	36.0	41.0	33.0	41.0
17	37.66855567484292	39.0	36.0	41.0	33.0	41.0
18	37.583933355791544	39.0	36.0	41.0	33.0	41.0
19	37.57588932930765	39.0	36.0	41.0	32.0	41.0
20	37.436401817225864	39.0	36.0	41.0	32.0	41.0
21	37.42250396429355	39.0	36.0	41.0	32.0	41.0
22	37.36742678256781	39.0	36.0	41.0	32.0	41.0
23	37.180499978486296	39.0	35.0	41.0	32.0	41.0
24	36.98444926491346	39.0	35.0	40.0	31.0	41.0
25	37.01545628467773	39.0	35.0	40.0	31.0	41.0
26	36.96879043658127	39.0	35.0	40.0	31.0	41.0
27	36.885945884113546	39.0	35.0	40.0	31.0	41.0
28	36.77470429151174	39.0	35.0	40.0	31.0	41.0
29	36.625916562508856	39.0	35.0	40.0	31.0	41.0
30	36.617534613451205	39.0	35.0	40.0	31.0	41.0
31	36.56581356958529	39.0	35.0	40.0	30.0	41.0
32	36.45775600520947	39.0	35.0	40.0	30.0	41.0
33	36.375139183171875	39.0	35.0	40.0	30.0	41.0
34	36.278024223381514	38.0	35.0	40.0	30.0	41.0
35	36.149417398394974	38.0	35.0	40.0	30.0	41.0
36	36.06864865602316	38.0	35.0	40.0	30.0	41.0
37	35.98474730842571	38.0	35.0	40.0	29.0	41.0
38	35.86349187359177	38.0	35.0	40.0	29.0	41.0
39	35.831646344087034	38.0	35.0	40.0	29.0	41.0
40	35.67226022976636	38.0	34.0	40.0	29.0	41.0
41	35.54290829599918	38.0	34.0	40.0	28.0	41.0
42	35.410257944066466	38.0	34.0	40.0	27.0	41.0
43	35.31045954321684	38.0	34.0	40.0	27.0	41.0
44	35.19164302782824	38.0	34.0	40.0	27.0	41.0
45	35.00786666960512	37.0	34.0	40.0	26.0	41.0
46	34.82632353882594	37.0	33.0	40.0	26.0	41.0
47	34.72721882375659	37.0	33.0	40.0	26.0	41.0
48	34.53759388632998	37.0	33.0	40.0	25.0	41.0
49	34.34693524165137	36.0	33.0	40.0	24.0	41.0
50	34.27240127571019	36.0	33.0	40.0	24.0	41.0
51	33.067529943403215	35.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	12.0
10	20.0
11	18.0
12	43.0
13	35.0
14	58.0
15	89.0
16	160.0
17	315.0
18	640.0
19	1041.0
20	1645.0
21	2501.0
22	3594.0
23	4652.0
24	5948.0
25	7459.0
26	8771.0
27	10733.0
28	13344.0
29	16523.0
30	20981.0
31	27148.0
32	35192.0
33	48017.0
34	69655.0
35	88499.0
36	97709.0
37	130740.0
38	179520.0
39	177786.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.989419455314987	32.40478086980431	18.01263746469916	27.593162210181543
2	23.50492873716655	31.66271549123133	18.973408012123237	25.858947759478884
3	21.503419629523517	31.127391563059813	20.291830774251977	27.07735803316469
4	21.30087597506929	32.004835860931216	18.90288504020964	27.791403123789856
5	18.62089809745393	34.804136088346816	16.216295633977378	30.358670180221875
6	19.207645025979108	40.85420949730344	18.184956988333276	21.753188488384172
7	84.99424377230736	7.4543410982063865	4.129476818196606	3.421938311289657
8	86.0322537651606	7.124709171449531	3.4750404300222164	3.36799663336765
9	80.00988580945575	9.698482811599769	5.855505566802151	4.436125812142334
10	37.86149582161886	32.392712206456	13.042447063169483	16.703344908755657
11	27.712589504880462	31.45471470204569	22.11587805822553	18.716817734848316
12	26.53447807228814	26.467943006524425	27.40457622725188	19.59300269393555
13	26.249552672369376	25.545372402220213	26.918051676967007	21.287023248443404
14	22.261331687797323	28.325362768278517	26.989624097867416	22.423681446056747
15	20.852551367904283	30.376615757896317	26.557775839795315	22.213057034404084
16	22.508791758886996	28.146011936432775	26.872400646040795	22.47279565863943
17	21.74857091284221	28.44730874054578	27.24589954044629	22.55822080616572
18	22.703779380636195	28.51541797978971	26.296463042079754	22.484339597494333
19	23.8957435398544	29.76604633737056	24.17164367848661	22.166566444288428
20	22.63199706993843	29.534432945981713	25.6236612966362	22.209908687443658
21	25.463095601654352	28.07517412982314	25.329395800734822	21.13233446778769
22	24.113189369921322	27.190068854348027	25.16736087717144	23.52938089855921
23	23.64754885447396	29.61828392002779	24.272915505713723	22.461251719784528
24	20.56542212511321	30.33453285352526	27.13497278254053	21.96507223882101
25	22.84702916733569	31.15562174080499	23.47512438594116	22.522224705918156
26	23.900990784788448	29.282040464654035	23.50010127182723	23.316867478730295
27	21.77134395585598	28.669791925749383	25.875843888166518	23.68302023022812
28	22.440157795149656	29.315307997535893	27.0480784064327	21.196455800881747
29	22.744812836020447	28.969724446179534	25.067243443829817	23.218219273970202
30	23.217274769882074	28.874014698582513	26.306222917657085	21.60248761387833
31	24.588274926249973	29.137006614676963	22.48601871587323	23.788699743199835
32	23.63317140335467	27.991218210878376	25.59049870865302	22.78511167711393
33	22.880191755318872	27.637763792120946	25.23683440009823	24.245210052461953
34	21.203067329498644	29.031746881299973	26.712674510248398	23.052511278952988
35	23.736856963251444	27.756246582731738	26.5782400950381	21.928656358978717
36	24.08286029420253	29.09345448172437	24.692800045336195	22.130885178736904
37	23.32001582569072	28.610393113095967	25.84394063896751	22.2256504222458
38	23.125447983536244	28.26407494744884	27.35808563713622	21.252391431878692
39	23.27342029067638	27.451066817367543	25.82557528169834	23.449937610257734
40	23.13258423664655	28.798874151126952	25.434865423909176	22.633676188317324
41	22.444670425792935	27.433855853983868	26.720755267446826	23.40071845277637
42	22.817854485502387	27.61939843485178	25.779294581380046	23.783452498265785
43	22.19689552000722	26.874919323609138	26.202432412861626	24.725752743522015
44	22.90275490853527	26.446849081889557	26.590938427778493	24.05945758179668
45	24.005935683469396	27.584241893793664	25.914148776185066	22.49567364655188
46	23.311200454201522	27.749005384722754	26.67101138547206	22.26878277560367
47	22.58025923488872	28.307836970198796	26.57278296030669	22.539120834605793
48	21.944083259084817	27.76285811134864	28.378254997213716	21.914803632352832
49	22.326082690283467	27.942313888093057	27.618768765459695	22.112834656163784
50	22.618144343312544	26.880901182833956	27.06980200045966	23.431152473393844
51	22.24800368566484	26.665869085436693	27.493884336029367	23.592242892869102
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15624.0
1	11868.0
2	8112.0
3	5124.5
4	2137.0
5	1931.0
6	1725.0
7	1860.0
8	1995.0
9	2299.0
10	2603.0
11	2811.0
12	3019.0
13	2963.5
14	2908.0
15	3023.0
16	3138.0
17	3223.0
18	3308.0
19	3215.0
20	3122.0
21	3401.0
22	3680.0
23	3989.0
24	4298.0
25	5098.5
26	7439.0
27	8979.0
28	11550.0
29	14121.0
30	17330.5
31	20540.0
32	22368.0
33	24196.0
34	25296.0
35	26396.0
36	27807.5
37	29219.0
38	31375.5
39	33532.0
40	35680.0
41	37828.0
42	39958.0
43	42088.0
44	47661.5
45	53235.0
46	55577.5
47	57920.0
48	63414.0
49	68908.0
50	69047.0
51	69186.0
52	64048.0
53	58910.0
54	54832.5
55	50755.0
56	48994.5
57	47234.0
58	46363.0
59	45492.0
60	43053.5
61	40615.0
62	37351.5
63	34088.0
64	32849.0
65	31610.0
66	28118.5
67	24627.0
68	22394.5
69	20162.0
70	18357.5
71	16553.0
72	14777.5
73	13002.0
74	10863.5
75	7466.5
76	6208.0
77	4608.0
78	3008.0
79	2585.5
80	2163.0
81	1655.5
82	1148.0
83	815.5
84	483.0
85	342.5
86	202.0
87	149.0
88	96.0
89	70.0
90	44.0
91	32.5
92	21.0
93	18.0
94	15.0
95	9.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	952881.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.27838974300351
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.04571758100647	16.606871964610363
2	8.829795404695426	3.757676559441814
3	3.1246722397635653	1.9946398121049858
4	1.6664738218490631	1.4183951791126783
5	1.028550267733609	1.0942946733553167
6	0.7285490042027674	0.9301409794982152
7	0.4959793633939242	0.7387549539147881
8	0.4071673078628848	0.6931091733852767
9	0.3009809717112839	0.5763951379170551
>10	3.1028718651799525	15.31147773324346
>50	1.2389627715916067	19.182619601145674
>100	1.0206789553894215	34.36819261824564
>500	0.007074012562104902	0.9419551625459089
>1k	0.0020211464463156864	0.9304508591110943
>5k	0.0	0.0
>10k+	5.052866115789216E-4	1.455025592367742
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13533	1.4202193138492634	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3091	0.32438468182280894	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2512	0.2636215854865403	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1529	0.16046075008316882	No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT	1522	0.1597261357924022	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.148346960428427E-4	0.0	0.0	0.194148062559753	0.0
2	3.148346960428427E-4	0.0	0.0	0.2810424386675776	0.0
3	3.148346960428427E-4	0.0	0.0	0.3918642516746582	0.0
4	3.148346960428427E-4	0.0	0.0	0.8754503447964647	0.0
5	3.148346960428427E-4	0.0	0.0	0.962659555600332	0.0
6	3.148346960428427E-4	0.0	0.0	1.480982410185532	0.0
7	3.148346960428427E-4	0.0	0.0	1.8399989085730537	0.0
8	3.148346960428427E-4	0.0	0.0	2.161025353638072	0.0
9	3.148346960428427E-4	0.0	0.0	2.6446114467598787	0.0
10	3.148346960428427E-4	0.0	0.0	3.402628449932363	0.0
11	3.148346960428427E-4	0.0	0.0	4.10785816906833	0.0
12	3.148346960428427E-4	0.0	0.0	4.400759381286855	0.0
13	4.197795947237903E-4	0.0	0.0	4.54191027001273	0.0
14	4.197795947237903E-4	0.0	0.0	4.687468844483204	0.0
15	4.197795947237903E-4	0.0	0.0	4.814557116785831	0.0
16	4.197795947237903E-4	0.0	0.0	5.05582543885333	0.0
17	4.197795947237903E-4	0.0	0.0	5.338127216305079	0.0
18	4.197795947237903E-4	0.0	0.0	5.7148794025696805	0.0
19	4.197795947237903E-4	0.0	0.0	5.872296750591103	0.0
20	4.197795947237903E-4	0.0	0.0	6.063086576393065	0.0
21	4.197795947237903E-4	0.0	0.0	6.264580781860484	0.0
22	4.197795947237903E-4	0.0	0.0	6.472686515944803	0.0
23	4.197795947237903E-4	0.0	0.0	6.691706519491941	0.0
24	4.197795947237903E-4	0.0	0.0	6.85667990021839	0.0
25	4.197795947237903E-4	0.0	0.0	7.0020285848915025	0.0
26	4.197795947237903E-4	0.0	0.0	7.133839377634773	0.0
27	4.197795947237903E-4	0.0	0.0	7.284540252140614	0.0
28	4.197795947237903E-4	0.0	0.0	7.450668026752553	0.0
29	4.197795947237903E-4	0.0	0.0	7.612388115619893	0.0
30	4.197795947237903E-4	0.0	0.0	7.820493849704213	0.0
31	4.197795947237903E-4	0.0	0.0	7.9748677956638865	0.0
32	4.197795947237903E-4	0.0	0.0	8.138371947808803	0.0
33	4.197795947237903E-4	0.0	0.0	8.306178840799639	0.0
34	4.197795947237903E-4	0.0	0.0	8.455410486723945	0.0
35	4.197795947237903E-4	0.0	0.0	8.634341538974962	0.0
36	4.197795947237903E-4	0.0	0.0	8.80130887277635	0.0
37	4.197795947237903E-4	0.0	0.0	8.963973465731817	0.0
38	4.197795947237903E-4	0.0	0.0	9.14815176291688	0.0
39	4.197795947237903E-4	0.0	0.0	9.322255349828572	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCATAT	60	0.0	45.000004	30
ATCCGAT	30	2.1651085E-6	45.000004	15
CTTACGT	60	0.0	45.000004	43
CTAGCGT	30	2.1651085E-6	45.000004	2
AAACTCG	35	1.2116288E-7	45.0	1
CGAAATT	25	3.8903017E-5	45.0	22
CAACGAA	20	7.0326787E-4	45.0	26
CCGATAG	40	6.8121153E-9	45.0	1
CGCATTA	25	3.8903017E-5	45.0	45
CGCATAT	25	3.8903017E-5	45.0	29
CCGGATA	25	3.8903017E-5	45.0	13
TAAACGT	20	7.0326787E-4	45.0	16
CAATTGA	25	3.8903017E-5	45.0	34
CGACAAT	25	3.8903017E-5	45.0	44
CGTTACG	45	3.8380676E-10	45.0	1
ACGATAG	35	1.2116288E-7	45.0	1
CGTAGAT	20	7.0326787E-4	45.0	13
AACGCGT	20	7.0326787E-4	45.0	39
CCATACG	45	3.8380676E-10	45.0	1
ACTACGC	25	3.8903017E-5	45.0	17
>>END_MODULE