FastQCFastQC Report
Sat 18 Jun 2016
SRR3553265_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553265_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944936
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA135631.4353353031316407No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32430.34319784620334076No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25590.27081199149995344No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15480.16382061853924498No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT14080.14900480032510138No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATT302.1650903E-645.00000422
TCGATTT253.8902763E-545.00000419
CCGTCAA351.2116288E-745.00000413
CGTTACG302.1650903E-645.0000041
ATCCGAT302.1650903E-645.00000415
GTTACGG302.1650903E-645.0000042
TACGAAT302.1650903E-645.00000411
GTCCGTT253.8902763E-545.00000417
GCGATTA253.8902763E-545.00000411
CGTACTC351.2116288E-745.00000445
ACGTCAT253.8902763E-545.00000413
ATGCGTA253.8902763E-545.00000412
ACCCGAT253.8902763E-545.00000430
CGTCATC253.8902763E-545.00000438
TATCGCG351.2116288E-745.0000041
CGTCAAT351.2116288E-745.00000414
ATACCCG351.2116288E-745.0000041
TAGTCCG253.8902763E-545.0000041
TACGTGA502.1827873E-1145.00000444
ACGAACT302.1650903E-645.00000433