##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553264_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	539625
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.656342830669445	31.0	31.0	34.0	30.0	34.0
2	31.85934120917304	33.0	31.0	34.0	30.0	34.0
3	32.023210562890895	33.0	31.0	34.0	30.0	34.0
4	35.751736854296965	37.0	35.0	37.0	35.0	37.0
5	35.48816492934908	37.0	35.0	37.0	33.0	37.0
6	34.16379893444522	37.0	35.0	37.0	32.0	37.0
7	34.92956034283067	37.0	35.0	37.0	32.0	37.0
8	35.59989437109103	37.0	35.0	37.0	32.0	37.0
9	37.295735001158214	39.0	37.0	39.0	34.0	39.0
10	36.738768589298125	39.0	37.0	39.0	32.0	39.0
11	36.72647486680565	39.0	37.0	39.0	32.0	39.0
12	36.63521334259903	39.0	35.0	39.0	32.0	39.0
13	36.59519481121149	39.0	35.0	39.0	32.0	39.0
14	37.78372202918693	40.0	37.0	41.0	33.0	41.0
15	37.784917303683116	40.0	37.0	41.0	33.0	41.0
16	37.691261524206624	40.0	36.0	41.0	33.0	41.0
17	37.617337966180216	39.0	36.0	41.0	33.0	41.0
18	37.52529627055826	39.0	36.0	41.0	33.0	41.0
19	37.483192958072735	39.0	36.0	41.0	32.0	41.0
20	37.36319666435024	39.0	35.0	41.0	32.0	41.0
21	37.32514060690294	39.0	35.0	41.0	32.0	41.0
22	37.312465137827196	39.0	35.0	41.0	32.0	41.0
23	37.13668380820014	39.0	35.0	40.0	32.0	41.0
24	36.89150428538337	39.0	35.0	40.0	31.0	41.0
25	36.95800602270095	39.0	35.0	40.0	31.0	41.0
26	36.86341626129256	39.0	35.0	40.0	31.0	41.0
27	36.82350521195274	39.0	35.0	40.0	31.0	41.0
28	36.75930321982858	39.0	35.0	40.0	31.0	41.0
29	36.51886958536021	39.0	35.0	40.0	30.0	41.0
30	36.555437572388236	39.0	35.0	40.0	30.0	41.0
31	36.455659022469305	38.0	35.0	40.0	30.0	41.0
32	36.34246745425064	38.0	35.0	40.0	30.0	41.0
33	36.29076302988186	38.0	35.0	40.0	30.0	41.0
34	36.195517257354645	38.0	35.0	40.0	30.0	41.0
35	36.06605513087793	38.0	35.0	40.0	30.0	41.0
36	35.94312902478573	38.0	35.0	40.0	29.0	41.0
37	35.892510539726665	38.0	35.0	40.0	29.0	41.0
38	35.80256474403521	38.0	35.0	40.0	29.0	41.0
39	35.72307250405374	38.0	35.0	40.0	29.0	41.0
40	35.54144452165856	38.0	34.0	40.0	28.0	41.0
41	35.43236136205699	38.0	34.0	40.0	27.0	41.0
42	35.32559277275886	38.0	34.0	40.0	27.0	41.0
43	35.236655084549454	38.0	34.0	40.0	27.0	41.0
44	35.133009034051426	38.0	34.0	40.0	27.0	41.0
45	34.93726013435256	37.0	34.0	40.0	26.0	41.0
46	34.75884364141765	37.0	33.0	40.0	26.0	41.0
47	34.675487607134585	37.0	33.0	40.0	25.0	41.0
48	34.52337827194811	37.0	33.0	40.0	25.0	41.0
49	34.350033819782254	36.0	33.0	40.0	24.0	41.0
50	34.245175816539266	36.0	33.0	40.0	24.0	41.0
51	33.0634829742877	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	6.0
12	16.0
13	26.0
14	22.0
15	58.0
16	77.0
17	187.0
18	353.0
19	628.0
20	1076.0
21	1520.0
22	2195.0
23	2916.0
24	3667.0
25	4461.0
26	5213.0
27	6220.0
28	7600.0
29	9503.0
30	12324.0
31	15268.0
32	19975.0
33	26562.0
34	39189.0
35	51873.0
36	55278.0
37	74012.0
38	101432.0
39	97949.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.27644197359277	32.739031735001156	19.462033819782256	26.522492471623814
2	23.826360898772293	32.335047486680565	19.341579800787585	24.497011813759556
3	20.559647903636787	32.26388695853602	21.191938846421127	25.98452629140607
4	22.514709288858004	33.93097058142228	17.704702339587676	25.849617790132033
5	19.29321287931434	36.59763724808895	15.556358582348853	28.55279129024786
6	18.110910354412788	41.780681028492005	17.888348390085707	22.220060227009498
7	83.07843409775307	9.094278434097754	4.113597405605745	3.713690062543433
8	83.91494093120222	8.152142691684041	3.9134584201992126	4.019457956914524
9	78.65406532314107	11.217419504285383	6.181885568681955	3.9466296038915916
10	35.166643502432244	35.97665045170257	12.674542506370164	16.182163539495022
11	25.95320824646745	31.688116747741486	23.28746814917767	19.07120685661339
12	25.667639564512395	27.05286078295112	27.878990039379197	19.400509613157286
13	24.62543432939541	28.888765346305306	25.499374565670607	20.986425758628677
14	20.5422283993514	30.388881167477415	27.222608292795925	21.846282140375262
15	19.74556404910818	31.66606439657169	27.138290479499656	21.450081074820478
16	22.4848737549224	30.014176511466296	26.02881630762103	21.47213342599027
17	20.56668983090109	30.128144544822792	27.334537873523278	21.97062775075284
18	23.346212647671994	29.625017373175815	24.617651146629605	22.411118832522586
19	22.646467454250637	32.40713458420199	24.325781792911744	20.620616168635628
20	23.36882094046792	32.05967106787121	23.741672457725272	20.829835533935604
21	23.983877692842253	30.68723650683345	24.469029418577716	20.85985638174658
22	22.816585591846188	29.18489691915682	24.4015751679407	23.59694232105629
23	23.121797544591153	31.50428538336808	23.13995830437804	22.233958767662727
24	20.684364141765116	32.20236275191105	25.822747278202456	21.290525828121382
25	23.033958767662728	31.357702107945336	22.946675932360435	22.661663192031504
26	23.666620338197824	30.461338892749595	23.82450776001853	22.04753300903405
27	21.424137132267777	30.260458651841553	25.354829742877	22.96057447301367
28	23.137549223998146	30.392216817234193	25.008292795923094	21.461941162844568
29	22.297336113041464	32.392680101922636	24.423627519110493	20.88635626592541
30	24.111373639101227	29.386518415566364	24.968079684966412	21.534028260365996
31	24.996432707899004	30.07403289321288	23.718322909427844	21.211211489460275
32	23.200185313875377	29.696548529071116	25.083900857076674	22.019365299976837
33	22.749501968959926	29.23270789900394	25.2668056520732	22.750984479962938
34	23.67551540421589	29.170257123002084	26.180588371554318	20.973639101227704
35	23.45962473940236	28.21737317581654	26.344035209636324	21.978966875144774
36	23.721287931433864	30.975028955293027	24.626360898772294	20.67732221450081
37	23.144961779013205	31.191475561732684	23.895668288163076	21.767894371091035
38	23.33861477878156	30.779337502895533	25.664489228630995	20.217558489691918
39	23.07769284225156	28.752374334028257	24.799629372249246	23.370303451470928
40	23.958489691915684	29.86240444753301	24.664720870975216	21.514384989576094
41	21.767523743340284	28.46977067407922	27.47797081306463	22.284734769515868
42	23.311558952976604	29.7995830437804	24.78869585360204	22.100162149640955
43	22.200602270094976	27.81672457725272	26.62515635858235	23.357516794069955
44	23.241139680333564	27.09437109103544	26.101644660643963	23.562844567987028
45	23.588788510539725	26.79064164929349	27.36882094046792	22.251748899698864
46	21.68839471855455	28.579661802177437	27.455918461894836	22.276025017373176
47	22.996895992587447	28.966967801714155	26.702246930738937	21.333889274959464
48	21.549038684271483	28.264628214037522	27.862497104470695	22.323835997220293
49	22.172063933287006	28.741996757007183	26.993375028955292	22.09256428075052
50	21.59073430623118	27.946444290016213	27.11271716469771	23.3501042390549
51	22.606810284920083	26.447440352096365	27.788000926569378	23.157748436414177
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13098.0
1	9840.0
2	6582.0
3	4078.0
4	1574.0
5	1346.5
6	1119.0
7	1198.0
8	1277.0
9	1371.5
10	1466.0
11	1614.0
12	1762.0
13	1877.5
14	1993.0
15	1899.5
16	1806.0
17	1796.0
18	1786.0
19	1916.5
20	2047.0
21	2079.0
22	2111.0
23	2188.0
24	2265.0
25	2561.0
26	4305.5
27	5754.0
28	7444.5
29	9135.0
30	9635.5
31	10136.0
32	10595.5
33	11055.0
34	13031.0
35	15007.0
36	16646.5
37	18286.0
38	18544.5
39	18803.0
40	19706.5
41	20610.0
42	21760.5
43	22911.0
44	25979.5
45	29048.0
46	32620.0
47	36192.0
48	38669.0
49	41146.0
50	40224.5
51	39303.0
52	37890.0
53	36477.0
54	34062.5
55	31648.0
56	29054.0
57	26460.0
58	26136.5
59	25813.0
60	22654.0
61	19495.0
62	19482.0
63	19469.0
64	17414.5
65	15360.0
66	14828.5
67	14297.0
68	12085.0
69	9873.0
70	8459.5
71	7046.0
72	6434.0
73	5822.0
74	4755.5
75	2788.0
76	1887.0
77	1595.5
78	1304.0
79	1044.5
80	785.0
81	591.0
82	397.0
83	364.5
84	332.0
85	280.5
86	229.0
87	151.0
88	73.0
89	49.0
90	25.0
91	16.5
92	8.0
93	6.0
94	4.0
95	3.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	539625.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.37994587659272
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.93711391571091	19.001026190428732
2	8.701499306449241	4.242841642728832
3	3.1171697279163655	2.2798928776426273
4	1.6692284822736856	1.6278280021399787
5	1.0805100657436226	1.317138846097158
6	0.7189922114274107	1.0517394720175188
7	0.5170475120378616	0.8823913251376991
8	0.4001443471752606	0.7804398021565905
9	0.3367499139351906	0.7388950208118483
>10	3.5927079601931604	21.07056149710911
>50	1.3897397205027409	24.15007583858086
>100	0.5322244973573683	18.73718237099499
>500	0.0030543730120939356	0.5972128987486406
>1k	0.0030543730120939356	1.3807755829235249
>5k	0.0	0.0
>10k+	7.635932530234839E-4	2.1419986324818763
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11506	2.132221450081075	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2606	0.48292795923094745	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1969	0.3648830206161686	No Hit
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCT	1737	0.32189020152883946	Illumina Single End Adapter 1 (95% over 23bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1105	0.20477183229094278	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGT	963	0.17845726198749132	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCC	869	0.16103775770210793	No Hit
AATCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCT	750	0.13898540653231412	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	626	0.11600648598563817	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3863794301598332	0.0
2	0.0	0.0	0.0	0.5101690989112809	0.0
3	0.0	0.0	0.0	0.6773222145008108	0.0
4	0.0	0.0	0.0	1.3679870280287236	0.0
5	0.0	0.0	0.0	1.4758397034977995	0.0
6	0.0	0.0	0.0	2.116469770674079	0.0
7	0.0	0.0	0.0	2.5747509844799628	0.0
8	0.0	0.0	0.0	2.977067407922168	0.0
9	0.0	0.0	0.0	3.6629140606902943	0.0
10	0.0	0.0	0.0	4.420477183229094	0.0
11	0.0	0.0	0.0	5.222700949733611	0.0
12	0.0	0.0	0.0	5.550891823025249	0.0
13	0.0	0.0	0.0	5.700440120454019	0.0
14	0.0	0.0	0.0	5.875932360435487	0.0
15	0.0	0.0	0.0	6.01658559184619	0.0
16	0.0	0.0	0.0	6.30789900393792	0.0
17	0.0	0.0	0.0	6.662775075283762	0.0
18	0.0	0.0	0.0	7.158675005791059	0.0
19	0.0	0.0	0.0	7.3593699328237205	0.0
20	0.0	0.0	0.0	7.5913829047949966	0.0
21	0.0	0.0	0.0	7.843595089182302	0.0
22	0.0	0.0	0.0	8.1045170257123	0.0
23	0.0	0.0	0.0	8.385823488533704	0.0
24	0.0	0.0	0.0	8.590780634700023	0.0
25	0.0	0.0	0.0	8.77201760481816	0.0
26	0.0	0.0	0.0	8.930831596015752	0.0
27	0.0	0.0	0.0	9.127820245540885	0.0
28	0.0	0.0	0.0	9.314246004169561	0.0
29	0.0	0.0	0.0	9.511790595320825	0.0
30	0.0	0.0	0.0	9.777901320361362	0.0
31	0.0	0.0	0.0	9.965068334491544	0.0
32	0.0	0.0	0.0	10.167060458651841	0.0
33	0.0	0.0	0.0	10.373870743571924	0.0
34	0.0	0.0	0.0	10.550845494556405	0.0
35	0.0	0.0	0.0	10.749872596710679	0.0
36	0.0	0.0	0.0	10.93815149409312	0.0
37	0.0	0.0	0.0	11.122538800092657	0.0
38	0.0	0.0	0.0	11.325272179754458	0.0
39	0.0	0.0	0.0	11.529673384294648	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTC	25	3.8880447E-5	45.000004	22
AGCGTAA	30	2.1633532E-6	45.000004	13
CGCGGAA	30	2.1633532E-6	45.000004	40
TAGACAG	25	3.8880447E-5	45.000004	1
CATGACG	30	2.1633532E-6	45.000004	1
ATTACCG	25	3.8880447E-5	45.000004	1
TCGATAG	30	2.1633532E-6	45.000004	1
TAGCCCG	25	3.8880447E-5	45.000004	1
CTGCGTT	25	3.8880447E-5	45.000004	24
CATTAAG	25	3.8880447E-5	45.000004	29
ACGATAC	30	2.1633532E-6	45.000004	24
ATATCCG	25	3.8880447E-5	45.000004	1
GTTCGCT	30	2.1633532E-6	45.000004	19
GAAACGT	25	3.8880447E-5	45.000004	13
AACGCCA	25	3.8880447E-5	45.000004	14
GAACGTT	25	3.8880447E-5	45.000004	21
TCATCGG	25	3.8880447E-5	45.000004	31
ATTGGGC	50	2.1827873E-11	45.000004	4
AGGATAC	25	3.8880447E-5	45.000004	28
TAGTGCG	30	2.1633532E-6	45.000004	1
>>END_MODULE