##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553263_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	533840
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.632352390229283	31.0	31.0	34.0	30.0	34.0
2	31.84184774464259	33.0	31.0	34.0	30.0	34.0
3	32.00804173535142	33.0	31.0	34.0	30.0	34.0
4	35.74457890004496	37.0	35.0	37.0	35.0	37.0
5	35.49363479694291	37.0	35.0	37.0	33.0	37.0
6	32.77932713921774	37.0	35.0	37.0	26.0	37.0
7	34.250269743743445	37.0	35.0	37.0	28.0	37.0
8	35.31804098606324	37.0	35.0	37.0	32.0	37.0
9	37.17061291772816	39.0	37.0	39.0	34.0	39.0
10	36.69225797991908	38.0	37.0	39.0	32.0	39.0
11	36.7325378390529	39.0	37.0	39.0	32.0	39.0
12	36.648248538888055	39.0	35.0	39.0	32.0	39.0
13	36.62268657275588	39.0	35.0	39.0	32.0	39.0
14	37.78263150007493	40.0	37.0	41.0	33.0	41.0
15	37.83382848793646	40.0	37.0	41.0	33.0	41.0
16	37.76298141765323	40.0	37.0	41.0	33.0	41.0
17	37.70313015135621	39.0	36.0	41.0	33.0	41.0
18	37.601697137719164	39.0	36.0	41.0	33.0	41.0
19	37.564710400119885	39.0	36.0	41.0	33.0	41.0
20	37.427234751985615	39.0	36.0	41.0	32.0	41.0
21	37.37868649782706	39.0	35.0	41.0	32.0	41.0
22	37.32626442379739	39.0	35.0	41.0	32.0	41.0
23	37.144856136670164	39.0	35.0	40.0	32.0	41.0
24	36.965124381837256	39.0	35.0	40.0	31.0	41.0
25	37.006973999700286	39.0	35.0	40.0	31.0	41.0
26	36.933450097407466	39.0	35.0	40.0	31.0	41.0
27	36.8597950696838	39.0	35.0	40.0	31.0	41.0
28	36.816452495129624	39.0	35.0	40.0	31.0	41.0
29	36.555037089764724	39.0	35.0	40.0	30.0	41.0
30	36.58791960137869	39.0	35.0	40.0	30.0	41.0
31	36.20341300764274	38.0	35.0	40.0	30.0	41.0
32	36.25363217443429	38.0	35.0	40.0	30.0	41.0
33	36.252912857785105	38.0	35.0	40.0	30.0	41.0
34	36.20264311404166	38.0	35.0	40.0	30.0	41.0
35	36.158867825565714	38.0	35.0	40.0	30.0	41.0
36	36.016027274089616	38.0	35.0	40.0	30.0	41.0
37	35.9514817173685	38.0	35.0	40.0	29.0	41.0
38	35.84822793346321	38.0	35.0	40.0	29.0	41.0
39	35.75299527948449	38.0	35.0	40.0	29.0	41.0
40	35.59340626404915	38.0	34.0	40.0	28.0	41.0
41	35.540555222538586	38.0	34.0	40.0	28.0	41.0
42	35.43537951446126	38.0	34.0	40.0	28.0	41.0
43	35.36124681552525	38.0	34.0	40.0	27.0	41.0
44	35.1972351266297	38.0	34.0	40.0	27.0	41.0
45	35.018602952195415	37.0	34.0	40.0	26.0	41.0
46	34.73387344522703	37.0	33.0	40.0	25.0	41.0
47	34.7018694739997	37.0	33.0	40.0	26.0	41.0
48	34.65970515510265	37.0	33.0	40.0	26.0	41.0
49	34.52516109695789	37.0	33.0	40.0	26.0	41.0
50	34.41189120335681	36.0	33.0	40.0	25.0	41.0
51	33.28868387531845	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	9.0
11	7.0
12	11.0
13	17.0
14	21.0
15	47.0
16	100.0
17	194.0
18	329.0
19	652.0
20	1057.0
21	1593.0
22	2181.0
23	2894.0
24	3631.0
25	4271.0
26	5104.0
27	6176.0
28	7126.0
29	9291.0
30	11744.0
31	15168.0
32	19933.0
33	26689.0
34	38696.0
35	52004.0
36	56157.0
37	75595.0
38	98975.0
39	94145.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.2200284729507	32.79765472800839	19.424172036565263	26.55814476247565
2	23.970478045856435	32.154952794844895	19.33369548928518	24.54087367001349
3	20.581447624756482	32.29881612468155	21.172823317848042	25.94691293271392
4	22.467780608422	33.99988760677356	17.798029372096508	25.734302412707926
5	19.30297467405964	36.552150457065785	15.491533043608571	28.653341825265997
6	17.35032968679754	44.15405364903342	17.203656526300016	21.291960137869022
7	83.19533942754383	9.085493780908136	4.069384085119137	3.6497827064288924
8	83.84066386932415	8.214633598081821	3.8363554623108045	4.108347070283231
9	78.73857335531245	11.237449423048105	6.1035141615465305	3.920463060092912
10	35.12850292222389	35.96021279784205	12.685261501573505	16.22602277836056
11	25.87441930166342	31.711748838603327	23.33620560467556	19.077626255057694
12	25.70208302112993	27.102502622508617	27.85104151056496	19.344372845796492
13	24.585268994455266	28.91147160197812	25.64794695039712	20.85531245316949
14	20.512700434587142	30.411171886707628	27.279521954143565	21.796605724561665
15	19.71845496778061	31.776374943803386	27.071594485239025	21.433575603176983
16	22.433875318447473	30.145923872321294	26.09283680503522	21.327364004196014
17	20.531619961037016	30.123632549078376	27.48876067735651	21.855986812528098
18	23.209201258804136	29.655327438933014	24.61037014835906	22.525101153903794
19	22.567997901993106	32.41458114790949	24.39326389929567	20.624157050801738
20	23.249850142364753	32.08714221489585	23.88824366851491	20.774763974224484
21	24.01243818372546	30.80286228083321	24.568410010490034	20.616289524951295
22	23.000524501723362	29.217368499925072	24.311029521954143	23.471077476397422
23	22.981230331185373	31.793795893900796	23.226434886857486	21.998538888056345
24	20.69252959688296	32.12516858983965	25.962460662370745	21.21984115090664
25	22.912670463060092	31.559456016784054	22.976921924172036	22.550951595983815
26	23.590026974374346	30.510639892102503	23.922336280533493	21.97699685298966
27	21.334294919826167	30.343735950846696	25.37239622358759	22.949572905739547
28	22.892252360257757	30.46849243218942	25.08710475048704	21.55215045706579
29	22.22126479844148	32.386670163344824	24.533942754383336	20.85812228383036
30	24.06919676307508	29.497414955791996	24.940993556121686	21.49239472501124
31	25.178517907987413	30.056009291173385	23.59751985613667	21.167952944702535
32	23.19889854638094	29.865128128278133	25.008616814026674	21.92735651131425
33	22.593286377940956	29.264573655027725	25.26749587891503	22.874644088116288
34	23.556496328487935	29.29061141915181	26.159523452719917	20.99336879964034
35	23.3843473699985	28.336580248763678	26.4742244867376	21.804847894500227
36	23.702420200809232	31.100891652929718	24.530008991458114	20.666679154802935
37	23.027311554023676	31.079349617863027	24.110032968679754	21.78330585943354
38	23.349505469803685	30.691031020530495	25.79424546680653	20.165218042859284
39	23.002210400119885	28.809193765922377	24.832721414656074	23.355874419301664
40	23.815188071332237	29.977521354713023	24.745054698036864	21.46223587591788
41	21.639442529596884	28.64997002847295	27.4861381687397	22.22444927319047
42	23.30173834856886	29.869249213247418	24.754795444327886	22.074216993855835
43	22.215832459163796	27.934961786303013	26.60216544282931	23.24704031170388
44	23.044357860032967	27.26753334332384	26.161396673160496	23.52671212348269
45	23.393900794245468	26.929979019931068	27.423010639892105	22.253109545931366
46	21.486400419601377	28.669826165143114	27.518170238273644	22.32560317698187
47	23.02019331634947	29.107410460062937	26.70406863479694	21.16832758879065
48	21.416529297167692	28.477633747939457	27.813951745841454	22.2918852090514
49	22.158886557770117	28.871759328637793	26.96369698786153	22.005657125730558
50	21.4742244867376	28.174172036565263	27.074404315899898	23.27719916079724
51	22.531844747489885	26.600854188520906	27.79709276187622	23.070208302112995
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13093.0
1	9835.0
2	6577.0
3	4093.0
4	1609.0
5	1338.5
6	1068.0
7	1218.0
8	1368.0
9	1470.5
10	1573.0
11	1687.5
12	1802.0
13	1903.5
14	2005.0
15	1911.5
16	1818.0
17	1766.0
18	1714.0
19	1873.5
20	2033.0
21	2044.0
22	2055.0
23	2160.5
24	2266.0
25	2536.0
26	4255.0
27	5704.0
28	7473.0
29	9242.0
30	9673.5
31	10105.0
32	10606.0
33	11107.0
34	13209.5
35	15312.0
36	16719.5
37	18127.0
38	18473.0
39	18819.0
40	19700.0
41	20581.0
42	21797.5
43	23014.0
44	25840.0
45	28666.0
46	32220.5
47	35775.0
48	37976.5
49	40178.0
50	39591.5
51	39005.0
52	37415.5
53	35826.0
54	33460.5
55	31095.0
56	28554.5
57	26014.0
58	25728.5
59	25443.0
60	22492.5
61	19542.0
62	19121.0
63	18700.0
64	16928.0
65	15156.0
66	14539.5
67	13923.0
68	11821.0
69	9719.0
70	8348.5
71	6978.0
72	6306.0
73	5634.0
74	4600.5
75	2660.0
76	1753.0
77	1482.5
78	1212.0
79	1010.0
80	808.0
81	604.5
82	401.0
83	342.0
84	283.0
85	257.5
86	232.0
87	167.0
88	102.0
89	59.0
90	16.0
91	13.0
92	10.0
93	6.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	533840.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.7391425582663
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.45388978628979	19.161428211152693
2	8.596750204291272	4.253524576835335
3	3.16584630634841	2.3496096927094237
4	1.7268484557600958	1.7088300049428413
5	1.1060528466094768	1.368139952462405
6	0.817347425395996	1.2132284685902086
7	0.5896482596181652	1.0211174647749186
8	0.48050056997748564	0.9509737680000984
9	0.3881980624531768	0.8643318487084714
>10	3.827767655036365	22.136218915851718
>50	1.358288568397766	23.820697602198923
>100	0.481235309746766	16.93502867558134
>500	0.0038132750376130425	0.6777181198632788
>1k	0.0030506200300904345	1.3603417717745658
>5k	0.0	0.0
>10k+	7.626550075226086E-4	2.17881092655377
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11548	2.1631949647834556	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2556	0.47879514461261796	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1935	0.3624681552525101	No Hit
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCT	1645	0.30814476247564815	Illumina Single End Adapter 1 (95% over 23bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1074	0.20118387531844745	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGT	873	0.16353214446276038	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCC	821	0.15379139817173684	No Hit
AATCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCT	725	0.13580848194215495	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	643	0.12044807432938709	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.366214596133673	0.0
2	0.0	0.0	0.0	0.4862880263749438	0.0
3	0.0	0.0	0.0	0.6385808481942155	0.0
4	0.0	0.0	0.0	1.2820320695339427	0.0
5	0.0	0.0	0.0	1.385808481942155	0.0
6	0.0	0.0	0.0	1.9891727858534392	0.0
7	0.0	0.0	0.0	2.4293795893900794	0.0
8	0.0	0.0	0.0	2.8191967630750785	0.0
9	0.0	0.0	0.0	3.4828787651730857	0.0
10	0.0	0.0	0.0	4.23366551775813	0.0
11	0.0	0.0	0.0	5.009178780158849	0.0
12	0.0	0.0	0.0	5.327438933013637	0.0
13	0.0	0.0	0.0	5.472988161246816	0.0
14	0.0	0.0	0.0	5.663494680053949	0.0
15	0.0	0.0	0.0	5.798366551775813	0.0
16	0.0	0.0	0.0	6.08852839802188	0.0
17	0.0	0.0	0.0	6.4232728907537835	0.0
18	0.0	0.0	0.0	6.903941255806983	0.0
19	0.0	0.0	0.0	7.107747639742245	0.0
20	0.0	0.0	0.0	7.338153753933763	0.0
21	0.0	0.0	0.0	7.602090514011689	0.0
22	0.0	0.0	0.0	7.831560017982916	0.0
23	0.0	0.0	0.0	8.101865727558819	0.0
24	0.0	0.0	0.0	8.305859433538139	0.0
25	0.0	0.0	0.0	8.475385883410759	0.0
26	0.0	0.0	0.0	8.625243518657276	0.0
27	0.0	0.0	0.0	8.807320545481792	0.0
28	0.0	0.0	0.0	8.99127079274689	0.0
29	0.0	0.0	0.0	9.178780158849094	0.0
30	0.0	0.0	0.0	9.449835156601228	0.0
31	0.0	0.0	0.0	9.625917878015885	0.0
32	0.0	0.0	0.0	9.809680803236924	0.0
33	0.0	0.0	0.0	10.003184474748988	0.0
34	0.0	0.0	0.0	10.183950247265098	0.0
35	0.0	0.0	0.0	10.376891952644987	0.0
36	0.0	0.0	0.0	10.553723962235876	0.0
37	0.0	0.0	0.0	10.724561666416903	0.0
38	0.0	0.0	0.0	10.919189270193316	0.0
39	0.0	0.0	0.0	11.136295519256706	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATAC	20	7.0298894E-4	45.0	39
CTTCGAT	20	7.0298894E-4	45.0	24
GATACTA	20	7.0298894E-4	45.0	9
TCGCATA	25	3.887988E-5	45.0	37
GGTACGT	25	3.887988E-5	45.0	8
TCCGCGA	20	7.0298894E-4	45.0	23
TCCGCAC	25	3.887988E-5	45.0	31
ATCTAGA	25	3.887988E-5	45.0	21
GTCGACA	35	1.210301E-7	45.0	23
GTTTCGT	20	7.0298894E-4	45.0	11
ACGTTCC	20	7.0298894E-4	45.0	10
GACACGT	35	1.210301E-7	45.0	9
CGGTTAG	20	7.0298894E-4	45.0	1
GGCCGTA	25	3.887988E-5	45.0	42
CGCATAG	75	0.0	45.0	1
ATTACCG	20	7.0298894E-4	45.0	1
ACCGAAT	20	7.0298894E-4	45.0	17
CGCGCCA	20	7.0298894E-4	45.0	33
CGACCAT	20	7.0298894E-4	45.0	25
GCGCCAA	20	7.0298894E-4	45.0	34
>>END_MODULE