##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553262_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	681230
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.539350880025836	31.0	31.0	34.0	30.0	34.0
2	31.742031325690295	31.0	31.0	34.0	30.0	34.0
3	31.93201708674016	33.0	31.0	34.0	30.0	34.0
4	35.66000469738561	37.0	35.0	37.0	33.0	37.0
5	35.400521116216254	37.0	35.0	37.0	33.0	37.0
6	34.02222010187455	37.0	35.0	37.0	32.0	37.0
7	34.8969305520896	37.0	35.0	37.0	32.0	37.0
8	35.592567855203086	37.0	35.0	37.0	32.0	37.0
9	37.27209752946875	39.0	37.0	39.0	34.0	39.0
10	36.627200798555556	38.0	35.0	39.0	32.0	39.0
11	36.62018701466465	39.0	35.0	39.0	32.0	39.0
12	36.66884458993292	39.0	35.0	39.0	32.0	39.0
13	36.659338255802005	39.0	35.0	39.0	32.0	39.0
14	37.79427212542019	39.0	37.0	41.0	33.0	41.0
15	37.79299502370712	39.0	37.0	41.0	33.0	41.0
16	37.6814100964432	39.0	37.0	41.0	32.0	41.0
17	37.63070035083599	39.0	36.0	41.0	32.0	41.0
18	37.50785637743493	39.0	36.0	40.0	32.0	41.0
19	37.42452622462311	39.0	36.0	41.0	32.0	41.0
20	37.309541564522995	39.0	35.0	40.0	32.0	41.0
21	37.28740219896364	39.0	35.0	40.0	32.0	41.0
22	37.24944732322417	39.0	35.0	40.0	32.0	41.0
23	37.07333499699074	39.0	35.0	40.0	32.0	41.0
24	36.85431792492991	39.0	35.0	40.0	31.0	41.0
25	36.90752609250914	39.0	35.0	40.0	31.0	41.0
26	36.83019684981578	39.0	35.0	40.0	31.0	41.0
27	36.8052273094256	39.0	35.0	40.0	31.0	41.0
28	36.692396107041674	39.0	35.0	40.0	31.0	41.0
29	36.587364032705544	39.0	35.0	40.0	31.0	41.0
30	36.559756616708015	39.0	35.0	40.0	30.0	41.0
31	36.43757761695756	38.0	35.0	40.0	30.0	41.0
32	36.34690633119504	38.0	35.0	40.0	30.0	41.0
33	36.227763016895906	38.0	35.0	40.0	30.0	41.0
34	36.171624855041614	38.0	35.0	40.0	30.0	41.0
35	36.05714956769373	38.0	35.0	40.0	30.0	41.0
36	36.01963507185532	38.0	35.0	40.0	30.0	41.0
37	35.97297535340487	38.0	35.0	40.0	30.0	41.0
38	35.86321947066336	38.0	35.0	40.0	29.0	41.0
39	35.782816376260584	38.0	35.0	40.0	29.0	41.0
40	35.67196835136444	38.0	35.0	40.0	28.0	41.0
41	35.61833007941517	38.0	35.0	40.0	28.0	41.0
42	35.55985496821925	38.0	34.0	40.0	28.0	41.0
43	35.50064295465555	38.0	34.0	40.0	28.0	41.0
44	35.3540243383292	38.0	34.0	40.0	27.0	41.0
45	35.15502546863761	38.0	34.0	40.0	26.0	41.0
46	34.96936717408217	38.0	34.0	40.0	26.0	41.0
47	34.88548654639402	37.0	34.0	40.0	26.0	41.0
48	34.765484491287815	37.0	33.0	40.0	25.0	41.0
49	34.61175667542533	37.0	33.0	40.0	24.0	41.0
50	34.52172100465335	37.0	33.0	40.0	24.0	41.0
51	33.361287670830706	36.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	6.0
11	3.0
12	10.0
13	14.0
14	19.0
15	24.0
16	99.0
17	180.0
18	446.0
19	853.0
20	1307.0
21	2102.0
22	2864.0
23	3739.0
24	4575.0
25	5146.0
26	6180.0
27	7409.0
28	9215.0
29	11802.0
30	14951.0
31	19436.0
32	25123.0
33	33328.0
34	47697.0
35	68155.0
36	71687.0
37	92198.0
38	128696.0
39	123946.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.79258106660012	34.829939961540155	20.287861661993748	25.08961730986598
2	21.425803326336183	35.25696167226928	18.614418037960746	24.702816963433786
3	20.486766583973107	35.428122660481776	19.052889626117462	25.03222112942765
4	19.252968894499656	35.136884752579896	19.132304801608853	26.4778415513116
5	17.014517857404986	39.16665443389164	17.06721665223199	26.75161105647138
6	18.07598021226311	42.80977056207155	17.727933297124316	21.386315928541023
7	81.71278422852781	10.737342747676996	4.16966369654889	3.3802093272463045
8	83.42630242355739	9.609529821058967	3.2331224402918255	3.7310453150918197
9	76.44011567312067	14.210178647446531	5.8702640811473366	3.4794415982854545
10	29.73415733305932	46.456703316060654	11.378682676922624	12.4304566739574
11	19.163718567884562	31.29442332251956	32.98827121530174	16.553586894294146
12	23.146514393083102	28.49610263787561	31.34888363695081	17.008499332090484
13	23.07370491610763	30.365662111181248	27.602571818622195	18.958061154088927
14	17.89924107863717	32.70173069301117	30.53462119988844	18.86440702846322
15	17.126521145574916	35.597228542489326	27.772852047032575	19.50339826490319
16	18.832993262187514	34.57995097103768	27.17540331459272	19.411652452182082
17	18.920188482597656	33.62755603834241	28.397457540037873	19.054797939022063
18	19.365852942471705	32.10868575958193	27.49673384906713	21.028727448879234
19	18.23627849624943	35.806702582094154	26.870513629757937	19.08650529189848
20	19.96858623372429	34.83992190596421	27.21474391908753	17.976747941223962
21	19.896363929950237	34.17318673575738	27.113016161942372	18.817433172350015
22	19.643292280140333	32.67428034584501	26.72694978201195	20.9554775920027
23	18.806130088222773	34.50890301366646	25.61308221892753	21.071884679183242
24	16.980755398323623	35.91562321095665	27.791935176078564	19.311686214641163
25	18.57830688607372	35.347973518488615	26.170749966971506	19.902969628466156
26	19.67162338710861	35.22085052038225	24.35609118799818	20.75143490451096
27	16.712417245276924	34.534885427829074	26.475346065205585	22.277351261688416
28	17.67567488219838	35.78791303964887	27.498642161971727	19.037769916181023
29	18.734201371049426	33.894426258385565	25.73580141802328	21.635570952541727
30	20.447719566079	32.92250781674325	26.359085771325397	20.270686845852357
31	19.871409068890095	35.6195411241431	23.13682603525975	21.37222377170706
32	20.86079591327452	33.07531964241152	25.237878543223285	20.826005901090674
33	18.74491728197525	34.17303994245703	25.541447088354886	21.540595687212836
34	17.82055986964755	35.0924063825727	25.399791553513495	21.687242194266254
35	19.43646051994187	32.82914727771825	27.19316530393553	20.541226898404357
36	19.824288419476535	34.6738986832641	25.4096267046372	20.09218619262217
37	21.076288478193856	33.92231698545278	24.54721606505879	20.454178471294572
38	20.27509064486297	33.63137266415161	27.057087914507584	19.03644877647784
39	19.029255904760507	32.386271890550915	27.020976762620553	21.563495442068024
40	21.007736006928642	32.296434390734404	25.836208035465262	20.859621566871688
41	18.382925003302848	31.921671094931227	28.670493078695884	21.024910823070034
42	19.686889890345405	32.246965048515186	26.599679990605228	21.46646507053418
43	19.23329859225225	31.447528734788545	26.66162676335452	22.657545909604686
44	20.867254818490082	31.081573037006592	25.99650631945158	22.054665825051746
45	21.98449862748264	31.38235250943147	26.12289535105618	20.51025351202971
46	19.03644877647784	32.37687711932827	27.34039898419036	21.246275120003524
47	19.041880128590932	32.68954684908181	27.239698780147677	21.028874242179587
48	19.668247141200474	31.266239008851638	29.166360847290928	19.89915300265696
49	19.372458640987624	30.52728153487075	27.84155131159814	22.25870851254349
50	18.301014341705443	30.769050100553414	27.319701128840478	23.610234428900664
51	19.417670977496588	29.489599694669938	28.95747398088751	22.135255346945964
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	21797.0
1	16649.0
2	11501.0
3	6980.0
4	2459.0
5	2227.5
6	1996.0
7	2205.0
8	2414.0
9	2542.5
10	2671.0
11	3130.0
12	3589.0
13	4052.0
14	4515.0
15	4463.0
16	4411.0
17	4129.0
18	3847.0
19	4171.0
20	4495.0
21	4254.0
22	4013.0
23	4329.5
24	4646.0
25	5567.0
26	7808.0
27	9128.0
28	9950.0
29	10772.0
30	13545.5
31	16319.0
32	18675.5
33	21032.0
34	23570.0
35	26108.0
36	26930.0
37	27752.0
38	28019.5
39	28287.0
40	32271.5
41	36256.0
42	35480.0
43	34704.0
44	39918.5
45	45133.0
46	49712.5
47	54292.0
48	56941.5
49	59591.0
50	58128.5
51	56666.0
52	50645.0
53	44624.0
54	38237.5
55	31851.0
56	28256.5
57	24662.0
58	22183.0
59	19704.0
60	18164.5
61	16625.0
62	14479.5
63	12334.0
64	10553.0
65	8772.0
66	7243.5
67	5715.0
68	4655.0
69	3595.0
70	3093.0
71	2591.0
72	2521.5
73	2452.0
74	1878.0
75	1082.5
76	861.0
77	681.0
78	501.0
79	439.0
80	377.0
81	291.0
82	205.0
83	143.0
84	81.0
85	63.5
86	46.0
87	39.5
88	33.0
89	19.0
90	5.0
91	4.0
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	3.0
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	681230.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.914355436354793
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.17209486324151	19.739507406177754
2	9.790905960411292	5.074500342040458
3	3.700506492359169	2.8768872161260206
4	1.8931195495032984	1.9623589155736132
5	1.2197784771524471	1.5804886505272044
6	0.8314400842112423	1.2927740319769734
7	0.6161151179785911	1.1176358309906214
8	0.4818717936883699	0.9989917549115395
9	0.383238693038585	0.8938245337529365
>10	3.5970489678698607	20.372215249459554
>50	0.7635334394705092	14.1321683841715
>100	0.5404982541927049	23.31891853396532
>500	0.004055185186869169	0.6428353129623351
>1k	0.005213809525974646	3.1310073066656843
>5k	0.0	0.0
>10k+	5.793121695527384E-4	2.8658865306984995
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19090	2.8022841037535047	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4822	0.7078372943058878	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3446	0.5058497130190978	No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT	2911	0.42731529732982987	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2056	0.3018070255273549	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGT	1824	0.26775097984527985	No Hit
GGTGTAGGGATTGAGAATATTTGAATCTACGTTTTATTAGGGATATTGGCC	1659	0.24353008528690748	No Hit
AATCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT	1497	0.21974957062959646	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCC	1420	0.20844648650235603	No Hit
ACTGCAGGGACCATCCCCAAGGAGACATAAAAAAGAGAAGAAGAAAGATAA	1221	0.17923461973195542	No Hit
AAACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT	828	0.1215448526929231	No Hit
AAAAACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTT	697	0.10231493034657897	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.935866007075437E-4	0.0	0.0	0.5126022048353713	0.0
2	2.935866007075437E-4	0.0	0.0	0.735434434772397	0.0
3	2.935866007075437E-4	0.0	0.0	0.9973136826035259	0.0
4	2.935866007075437E-4	0.0	0.0	2.194559840288889	0.0
5	2.935866007075437E-4	0.0	0.0	2.3742348399219058	0.0
6	2.935866007075437E-4	0.0	0.0	3.354814086285102	0.0
7	2.935866007075437E-4	0.0	0.0	4.060743067686391	0.0
8	2.935866007075437E-4	0.0	0.0	4.520646477694758	0.0
9	2.935866007075437E-4	0.0	0.0	5.639211426390499	0.0
10	2.935866007075437E-4	0.0	0.0	7.0363900591577	0.0
11	2.935866007075437E-4	0.0	0.0	8.511075554511692	0.0
12	2.935866007075437E-4	0.0	0.0	9.162984601382792	0.0
13	2.935866007075437E-4	0.0	0.0	9.42750612862029	0.0
14	2.935866007075437E-4	0.0	0.0	9.736065645963919	0.0
15	2.935866007075437E-4	0.0	0.0	9.969466993526416	0.0
16	2.935866007075437E-4	0.0	0.0	10.421003185414618	0.0
17	2.935866007075437E-4	0.0	0.0	11.160988212497982	0.0
18	2.935866007075437E-4	0.0	0.0	11.955433554012595	0.0
19	2.935866007075437E-4	0.0	0.0	12.419447176430868	0.0
20	2.935866007075437E-4	0.0	0.0	12.795678405237584	0.0
21	2.935866007075437E-4	0.0	0.0	13.229305814482627	0.0
22	2.935866007075437E-4	0.0	0.0	13.7088795267384	0.0
23	2.935866007075437E-4	0.0	0.0	14.179792434273299	0.0
24	2.935866007075437E-4	0.0	0.0	14.49187499082542	0.0
25	2.935866007075437E-4	0.0	0.0	14.793094843151358	0.0
26	2.935866007075437E-4	0.0	0.0	15.084479544353595	0.0
27	2.935866007075437E-4	0.0	0.0	15.40023193341456	0.0
28	2.935866007075437E-4	0.0	0.0	15.691176254715735	0.0
29	2.935866007075437E-4	0.0	0.0	16.041425069359835	0.0
30	2.935866007075437E-4	0.0	0.0	16.42264727037858	0.0
31	4.403799010613156E-4	0.0	0.0	16.72474788250664	0.0
32	4.403799010613156E-4	0.0	0.0	17.04490407057822	0.0
33	4.403799010613156E-4	0.0	0.0	17.362271185943072	0.0
34	4.403799010613156E-4	0.0	0.0	17.660555172261937	0.0
35	4.403799010613156E-4	0.0	0.0	17.993482377464293	0.0
36	4.403799010613156E-4	0.0	0.0	18.294408643189524	0.0
37	4.403799010613156E-4	0.0	0.0	18.62117052977702	0.0
38	4.403799010613156E-4	0.0	0.0	18.9667219588098	0.0
39	4.403799010613156E-4	0.0	0.0	19.314034907446825	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTACG	35	1.2109558E-7	45.000004	15
CTCGTGG	35	1.2109558E-7	45.000004	2
GACCGTT	35	1.2109558E-7	45.000004	9
TAGCGAT	35	1.2109558E-7	45.000004	45
ATTCGCG	35	1.2109558E-7	45.000004	1
CCGTAGT	35	1.2109558E-7	45.000004	27
CGTAGTG	35	1.2109558E-7	45.000004	28
CGTAGAC	35	1.2109558E-7	45.000004	37
TACGTCG	35	1.2109558E-7	45.000004	1
AGGTACG	45	3.8380676E-10	45.0	6
GCCCATA	25	3.8891274E-5	45.0	36
TCGCAAT	40	6.8084773E-9	45.0	29
ACACGCC	20	7.031261E-4	45.0	25
GCGACAT	25	3.8891274E-5	45.0	34
TTGGACG	20	7.031261E-4	45.0	39
TACCACG	50	2.1827873E-11	45.0	1
ACGCATT	20	7.031261E-4	45.0	15
CTATCCG	45	3.8380676E-10	45.0	1
CGGCCGA	20	7.031261E-4	45.0	34
CGTTGTA	20	7.031261E-4	45.0	20
>>END_MODULE