FastQCFastQC Report
Sat 18 Jun 2016
SRR3553259_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553259_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968293
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA205282.120019456920581No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47820.49385878034851016No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT33260.3434910714009086Illumina PCR Primer Index 8 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32820.3389469922843602No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGT25170.25994198037164373No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCC23660.2443475270398526No Hit
AATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT22310.23040546611407914No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20310.2097505610388591No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGCG453.8380676E-1045.00000421
CGCATTG453.8380676E-1045.00000412
CTTGACG453.8380676E-1045.0000041
TCGCGGA453.8380676E-1045.00000423
TCGCGCG453.8380676E-1045.0000041
CGGACAA351.2116652E-745.037
AGTCCCG207.032737E-445.01
CGAACCG207.032737E-445.01
AGTCACG351.2116652E-745.01
CTCCGCA207.032737E-445.020
ACTTGCG351.2116652E-745.01
CCCTACG207.032737E-445.032
GACGTAC207.032737E-445.09
CGTTGAC502.1827873E-1145.043
ATTCGTC351.2116652E-745.037
TCCTCGA207.032737E-445.026
TTCCGTC406.8139343E-945.040
ATTCGAT207.032737E-445.011
TCGATTG207.032737E-445.013
TAACGCG207.032737E-445.01