Basic Statistics
Measure | Value |
---|---|
Filename | SRR3553259_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 968293 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 43 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 20528 | 2.120019456920581 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 4782 | 0.49385878034851016 | No Hit |
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT | 3326 | 0.3434910714009086 | Illumina PCR Primer Index 8 (95% over 21bp) |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 3282 | 0.3389469922843602 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGT | 2517 | 0.25994198037164373 | No Hit |
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCC | 2366 | 0.2443475270398526 | No Hit |
AATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT | 2231 | 0.23040546611407914 | No Hit |
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2031 | 0.2097505610388591 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TTTCGCG | 45 | 3.8380676E-10 | 45.000004 | 21 |
CGCATTG | 45 | 3.8380676E-10 | 45.000004 | 12 |
CTTGACG | 45 | 3.8380676E-10 | 45.000004 | 1 |
TCGCGGA | 45 | 3.8380676E-10 | 45.000004 | 23 |
TCGCGCG | 45 | 3.8380676E-10 | 45.000004 | 1 |
CGGACAA | 35 | 1.2116652E-7 | 45.0 | 37 |
AGTCCCG | 20 | 7.032737E-4 | 45.0 | 1 |
CGAACCG | 20 | 7.032737E-4 | 45.0 | 1 |
AGTCACG | 35 | 1.2116652E-7 | 45.0 | 1 |
CTCCGCA | 20 | 7.032737E-4 | 45.0 | 20 |
ACTTGCG | 35 | 1.2116652E-7 | 45.0 | 1 |
CCCTACG | 20 | 7.032737E-4 | 45.0 | 32 |
GACGTAC | 20 | 7.032737E-4 | 45.0 | 9 |
CGTTGAC | 50 | 2.1827873E-11 | 45.0 | 43 |
ATTCGTC | 35 | 1.2116652E-7 | 45.0 | 37 |
TCCTCGA | 20 | 7.032737E-4 | 45.0 | 26 |
TTCCGTC | 40 | 6.8139343E-9 | 45.0 | 40 |
ATTCGAT | 20 | 7.032737E-4 | 45.0 | 11 |
TCGATTG | 20 | 7.032737E-4 | 45.0 | 13 |
TAACGCG | 20 | 7.032737E-4 | 45.0 | 1 |