##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553259_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	968293
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.560687725719383	31.0	31.0	34.0	30.0	34.0
2	31.77075327406064	31.0	31.0	34.0	30.0	34.0
3	31.944165660600664	33.0	31.0	34.0	30.0	34.0
4	35.6708589239001	37.0	35.0	37.0	33.0	37.0
5	35.44068272723236	37.0	35.0	37.0	33.0	37.0
6	32.70189291877562	37.0	35.0	37.0	26.0	37.0
7	34.263177571251674	37.0	35.0	37.0	28.0	37.0
8	35.373236200199734	37.0	35.0	37.0	32.0	37.0
9	37.2193447644463	39.0	37.0	39.0	34.0	39.0
10	36.633597475144406	38.0	35.0	39.0	32.0	39.0
11	36.66519947990949	39.0	35.0	39.0	32.0	39.0
12	36.73839943075082	39.0	35.0	39.0	32.0	39.0
13	36.75430990413026	39.0	37.0	39.0	32.0	39.0
14	37.87749265976311	40.0	37.0	41.0	33.0	41.0
15	37.90762093705108	40.0	37.0	41.0	33.0	41.0
16	37.8184392534078	40.0	37.0	41.0	33.0	41.0
17	37.813747491719965	39.0	37.0	41.0	33.0	41.0
18	37.66823368546504	39.0	37.0	41.0	33.0	41.0
19	37.6081134532626	39.0	36.0	41.0	32.0	41.0
20	37.54319715210169	39.0	36.0	41.0	32.0	41.0
21	37.50940882563439	39.0	36.0	41.0	32.0	41.0
22	37.426781976116736	39.0	36.0	41.0	32.0	41.0
23	37.25257540847657	39.0	36.0	40.0	32.0	41.0
24	37.14925854054506	39.0	35.0	40.0	31.0	41.0
25	37.18795550520349	39.0	35.0	40.0	32.0	41.0
26	37.08109115732531	39.0	35.0	40.0	31.0	41.0
27	37.01846032141098	39.0	35.0	40.0	31.0	41.0
28	36.986233505767366	39.0	35.0	40.0	31.0	41.0
29	36.77687538792494	39.0	35.0	40.0	31.0	41.0
30	36.79453739725476	39.0	35.0	40.0	31.0	41.0
31	36.355383132997964	39.0	35.0	40.0	30.0	41.0
32	36.42310437026809	39.0	35.0	40.0	30.0	41.0
33	36.420734219910706	39.0	35.0	40.0	30.0	41.0
34	36.289831693505995	38.0	35.0	40.0	30.0	41.0
35	36.308914760304994	38.0	35.0	40.0	30.0	41.0
36	36.1957723540292	38.0	35.0	40.0	30.0	41.0
37	36.13187020870749	38.0	35.0	40.0	30.0	41.0
38	36.07046317591886	38.0	35.0	40.0	30.0	41.0
39	35.948861553269516	38.0	35.0	40.0	29.0	41.0
40	35.7581930262844	38.0	35.0	40.0	28.0	41.0
41	35.67643678101567	38.0	35.0	40.0	28.0	41.0
42	35.582126484442206	38.0	34.0	40.0	28.0	41.0
43	35.55041604142548	38.0	34.0	40.0	28.0	41.0
44	35.384827732927945	38.0	34.0	40.0	27.0	41.0
45	35.17418178175408	38.0	34.0	40.0	26.0	41.0
46	34.859159366018346	37.0	33.0	40.0	25.0	41.0
47	34.80038273539105	37.0	33.0	40.0	26.0	41.0
48	34.7214655068249	37.0	33.0	40.0	26.0	41.0
49	34.56524419777898	37.0	33.0	40.0	26.0	41.0
50	34.42812867592764	37.0	33.0	40.0	25.0	41.0
51	33.28544665715852	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	7.0
11	10.0
12	6.0
13	11.0
14	26.0
15	52.0
16	116.0
17	221.0
18	513.0
19	913.0
20	1648.0
21	2544.0
22	3566.0
23	4693.0
24	5922.0
25	6892.0
26	8564.0
27	10298.0
28	12793.0
29	16525.0
30	21657.0
31	27643.0
32	36244.0
33	47643.0
34	66666.0
35	92965.0
36	107285.0
37	138605.0
38	179712.0
39	174515.0
40	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.258640721351906	33.92733397845487	19.05776454027861	26.756260759914614
2	21.805899660536635	33.57496129787162	19.344764446298797	25.274374595292954
3	21.393317931659116	33.035558451832244	20.5468799216766	25.024243694832037
4	20.584678397964254	33.90926093651405	18.001885792833367	27.50417487268833
5	18.34021313796547	37.82191960491297	15.60509060790484	28.232776649216714
6	17.57598165018233	43.7949050545651	17.357969127113385	21.271144168139188
7	84.37022678053027	8.944193544722518	3.6426990590657993	3.0428806156814105
8	85.61943543947957	7.785866468104179	2.906145144083454	3.688552948332788
9	78.86961900994844	12.118955729309207	5.713353292856604	3.298071967885754
10	29.7828240005866	45.896954744070236	11.181636136995724	13.138585118347443
11	19.005094532336802	31.31376556476191	31.799362383080325	17.881777519820965
12	23.376705191507117	28.01445430257164	31.213486000621714	17.395354505299533
13	22.52923443627084	29.810295024336643	29.33234052089605	18.328130018496466
14	19.151744358370866	31.868762864133064	29.405045786760827	19.574446990735243
15	17.52227889698676	34.566913114109056	28.505834494311124	19.40497349459306
16	19.88303127255903	33.04867431655501	28.084784254352762	18.9835101565332
17	18.678333934046822	31.987941666417086	29.351859406192133	19.981864993343954
18	19.862169818433056	32.018924024029914	27.6278977540889	20.49100840344813
19	19.43998355869556	34.40972928648663	27.267263111475554	18.883024043342253
20	20.104245305914638	34.22683010204556	26.727653716385436	18.94127087565437
21	20.893056130737286	33.009016898810586	27.15954778150828	18.938379188943845
22	19.78770888563689	31.996203628447173	26.8021146491816	21.413972836734334
23	19.01025825860561	34.99891045375728	25.651946260067977	20.338885027569134
24	17.801533213603733	35.700144481061	26.73570912936477	19.762613175970497
25	19.595721542962718	34.69445715294854	25.15137463557002	20.558446668518723
26	20.279089077376373	35.849995817381725	23.37525934815185	20.495655757090052
27	18.235802592810234	34.354477415410415	26.148696727127017	21.26102326465233
28	19.84936377728642	33.995185341626964	26.94618261208126	19.209268269005353
29	19.86392548536445	34.71356294014312	24.95566940998231	20.466842164510123
30	23.015760725317648	32.77406735357996	25.951855481760173	18.258316439342224
31	22.580458600857384	34.649119636308434	22.97486401326871	19.795557749565475
32	21.070688314384178	33.999935969794265	24.29367970232151	20.635696013500045
33	22.529854083423096	32.55863669364542	24.444770332946742	20.466738889984747
34	19.494719057144895	33.100311579243055	26.779497528124235	20.62547183548781
35	21.612879572608705	32.38255362787916	26.031583415350518	19.972983384161612
36	22.108287470837855	34.09670420007167	25.36887078601209	18.426137543078386
37	20.587879908250912	34.137910735696735	24.536271562429967	20.737937793622386
38	22.16983908796201	32.89479527374462	25.573354346256767	19.362011292036605
39	20.64674638771529	31.567407799085608	26.14198388297757	21.643861930221533
40	22.63436790310371	32.010042414847575	26.166563220017082	19.189026462031634
41	19.11590809806536	31.30963458374686	29.619133877865483	19.9553234403223
42	20.78017707450121	33.085336773063524	25.343258703718814	20.791227448716455
43	20.23251226643175	32.407546063020185	26.461721813541978	20.898219857006094
44	22.270635024729085	30.610672596001415	25.284082400678308	21.83460997859119
45	23.301211513457186	31.042566661124265	26.216031717672234	19.440190107746314
46	20.244801934951507	32.00322629617275	27.656504797618076	20.095466971257668
47	20.537172116291245	33.63578999331814	26.321784831657357	19.505253058733256
48	20.30356513989051	32.40196923864987	27.32654268904144	19.967922932418183
49	20.2085525765445	32.343515857286995	26.692127279656052	20.75580428651245
50	20.160943020346114	30.64547611105316	26.600212952071328	22.5933679165294
51	19.529109474095137	29.423325377752395	29.001552216116405	22.046012932036067
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	23296.0
1	17510.0
2	11724.0
3	7291.0
4	2858.0
5	2618.0
6	2378.0
7	2723.0
8	3068.0
9	3862.5
10	4657.0
11	5404.0
12	6151.0
13	7104.5
14	8058.0
15	7754.0
16	7450.0
17	7707.0
18	7964.0
19	7451.0
20	6938.0
21	6498.0
22	6058.0
23	7150.0
24	8242.0
25	8662.0
26	10426.5
27	11771.0
28	14594.5
29	17418.0
30	18767.0
31	20116.0
32	22899.5
33	25683.0
34	30723.5
35	35764.0
36	38609.5
37	41455.0
38	43924.0
39	46393.0
40	47285.0
41	48177.0
42	48880.0
43	49583.0
44	50378.0
45	51173.0
46	57904.5
47	64636.0
48	69258.5
49	73881.0
50	78251.5
51	82622.0
52	79088.0
53	75554.0
54	67058.0
55	58562.0
56	50628.5
57	42695.0
58	36232.5
59	29770.0
60	26978.0
61	24186.0
62	21525.0
63	18864.0
64	16550.5
65	14237.0
66	11120.5
67	8004.0
68	6980.5
69	5957.0
70	5131.5
71	4306.0
72	4069.5
73	3833.0
74	3306.5
75	2198.5
76	1617.0
77	1059.0
78	501.0
79	465.0
80	429.0
81	321.0
82	213.0
83	148.0
84	83.0
85	70.5
86	58.0
87	39.5
88	21.0
89	12.5
90	4.0
91	7.5
92	11.0
93	11.5
94	12.0
95	6.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	968293.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.34187176862514
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.85860133778012	18.97057075880654
2	9.866734913229948	5.0008306189218
3	3.768236844422164	2.8648252471546534
4	1.9655217983666717	1.992400054905827
5	1.2982949080243071	1.6450611538505477
6	0.9228825947887068	1.4032543424778867
7	0.7276283719378319	1.2907625427803409
8	0.5413323773523812	1.097470055285523
9	0.5116183530244744	1.1668830027139276
>10	4.243853085594587	22.71851881811132
>50	0.769004257432108	13.783345565833475
>100	0.5155589461963387	22.631402962691055
>500	0.007429992819482864	1.1392586534165392
>1k	0.0028894416520211137	2.1480742308244087
>5k	0.0	0.0
>10k+	4.127773788601591E-4	2.1473419922261243
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20528	2.120019456920581	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4782	0.49385878034851016	No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT	3326	0.3434910714009086	Illumina PCR Primer Index 8 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3282	0.3389469922843602	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGT	2517	0.25994198037164373	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCC	2366	0.2443475270398526	No Hit
AATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	2231	0.23040546611407914	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2031	0.2097505610388591	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.032745253761E-4	0.0	0.0	0.4180552787224528	0.0
2	1.032745253761E-4	0.0	0.0	0.5920728539811814	0.0
3	1.032745253761E-4	0.0	0.0	0.812770514709907	0.0
4	1.032745253761E-4	0.0	0.0	1.8127777439266834	0.0
5	1.032745253761E-4	0.0	0.0	1.9580850011308562	0.0
6	1.032745253761E-4	0.0	0.0	2.7424550213623355	0.0
7	1.032745253761E-4	0.0	0.0	3.349089583421547	0.0
8	1.032745253761E-4	0.0	0.0	3.822086909644085	0.0
9	1.032745253761E-4	0.0	0.0	4.8156911182875435	0.0
10	1.032745253761E-4	0.0	0.0	5.976909881616412	0.0
11	1.032745253761E-4	0.0	0.0	7.3100807296964865	0.0
12	1.032745253761E-4	0.0	0.0	7.856506243461432	0.0
13	1.032745253761E-4	0.0	0.0	8.095070397080223	0.0
14	1.032745253761E-4	0.0	0.0	8.354496004824986	0.0
15	1.032745253761E-4	0.0	0.0	8.568377546878889	0.0
16	1.032745253761E-4	0.0	0.0	8.973420235403953	0.0
17	1.032745253761E-4	0.0	0.0	9.547936420071197	0.0
18	1.032745253761E-4	0.0	0.0	10.228102444198193	0.0
19	1.032745253761E-4	0.0	0.0	10.542779923019168	0.0
20	1.032745253761E-4	0.0	0.0	10.856114833010256	0.0
21	1.032745253761E-4	0.0	0.0	11.219641162334128	0.0
22	1.032745253761E-4	0.0	0.0	11.630777047856382	0.0
23	1.032745253761E-4	0.0	0.0	12.049038875629588	0.0
24	1.032745253761E-4	0.0	0.0	12.339240291936429	0.0
25	1.032745253761E-4	0.0	0.0	12.585859858534555	0.0
26	1.032745253761E-4	0.0	0.0	12.821119227341311	0.0
27	1.032745253761E-4	0.0	0.0	13.087774051862402	0.0
28	1.032745253761E-4	0.0	0.0	13.366718544903247	0.0
29	1.032745253761E-4	0.0	0.0	13.64442374363958	0.0
30	1.032745253761E-4	0.0	0.0	14.012700701130752	0.0
31	1.032745253761E-4	0.0	0.0	14.288650232935693	0.0
32	1.032745253761E-4	0.0	0.0	14.550657703814858	0.0
33	1.032745253761E-4	0.0	0.0	14.838070707936543	0.0
34	1.032745253761E-4	0.0	0.0	15.118977416959536	0.0
35	1.032745253761E-4	0.0	0.0	15.428800993087837	0.0
36	2.065490507522E-4	0.0	0.0	15.705576721095785	0.0
37	2.065490507522E-4	0.0	0.0	15.997946902435523	0.0
38	2.065490507522E-4	0.0	0.0	16.282055121745174	0.0
39	2.065490507522E-4	0.0	0.0	16.572359812577393	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTCGCG	45	3.8380676E-10	45.000004	21
CGCATTG	45	3.8380676E-10	45.000004	12
CTTGACG	45	3.8380676E-10	45.000004	1
TCGCGGA	45	3.8380676E-10	45.000004	23
TCGCGCG	45	3.8380676E-10	45.000004	1
CGGACAA	35	1.2116652E-7	45.0	37
AGTCCCG	20	7.032737E-4	45.0	1
CGAACCG	20	7.032737E-4	45.0	1
AGTCACG	35	1.2116652E-7	45.0	1
CTCCGCA	20	7.032737E-4	45.0	20
ACTTGCG	35	1.2116652E-7	45.0	1
CCCTACG	20	7.032737E-4	45.0	32
GACGTAC	20	7.032737E-4	45.0	9
CGTTGAC	50	2.1827873E-11	45.0	43
ATTCGTC	35	1.2116652E-7	45.0	37
TCCTCGA	20	7.032737E-4	45.0	26
TTCCGTC	40	6.8139343E-9	45.0	40
ATTCGAT	20	7.032737E-4	45.0	11
TCGATTG	20	7.032737E-4	45.0	13
TAACGCG	20	7.032737E-4	45.0	1
>>END_MODULE