FastQCFastQC Report
Sat 18 Jun 2016
SRR3553258_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553258_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences841715
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA219852.611929215946015No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51530.6122024675810696No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40830.48508105475131136No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT34850.41403562963710994No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21550.2560249015403076No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCTA302.1648066E-645.0000046
CGTGCCG302.1648066E-645.0000041
CGACCGG302.1648066E-645.0000042
CGACATG302.1648066E-645.0000041
CCTGTCG302.1648066E-645.00000419
CCGTAGC302.1648066E-645.00000430
TACGAGC302.1648066E-645.00000424
CCATACG302.1648066E-645.0000041
ACGTCCA302.1648066E-645.00000413
TTACGTC302.1648066E-645.00000411
CGACTCG302.1648066E-645.0000041
TATCGAG302.1648066E-645.0000041
TTTTCGC302.1648066E-645.00000432
TACCGAG302.1648066E-645.0000041
CGGACGA302.1648066E-645.00000430
ATTTACG502.1827873E-1145.01
CGGCGTA207.032209E-445.024
ACTATCG253.8899125E-545.01
AACCGCG453.8380676E-1045.01
GGTACTA351.2114106E-745.08
Produced by FastQC (version 0.11.5)