##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553258_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	841715
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.506833072952247	31.0	31.0	34.0	30.0	34.0
2	31.709275704959516	31.0	31.0	34.0	30.0	34.0
3	31.898289801179736	33.0	31.0	34.0	30.0	34.0
4	35.641085165406345	37.0	35.0	37.0	33.0	37.0
5	35.37342212031388	37.0	35.0	37.0	33.0	37.0
6	34.0094069845494	37.0	35.0	37.0	32.0	37.0
7	34.916269758766326	37.0	35.0	37.0	32.0	37.0
8	35.61200406313301	37.0	35.0	37.0	33.0	37.0
9	37.29253726023654	39.0	37.0	39.0	34.0	39.0
10	36.59542244108754	38.0	35.0	39.0	32.0	39.0
11	36.628902894685254	39.0	35.0	39.0	32.0	39.0
12	36.69655643537302	39.0	35.0	39.0	32.0	39.0
13	36.66818459930024	39.0	35.0	39.0	32.0	39.0
14	37.82864271160666	39.0	37.0	41.0	33.0	41.0
15	37.81854309356493	39.0	37.0	41.0	33.0	41.0
16	37.7217371675686	39.0	37.0	41.0	33.0	41.0
17	37.67786364743411	39.0	37.0	41.0	33.0	41.0
18	37.54148494442893	39.0	36.0	41.0	32.0	41.0
19	37.44857226020684	39.0	36.0	41.0	32.0	41.0
20	37.35839803258823	39.0	36.0	40.0	32.0	41.0
21	37.31603690085124	39.0	36.0	40.0	32.0	41.0
22	37.28364113743963	39.0	35.0	40.0	32.0	41.0
23	37.104295396898	39.0	35.0	40.0	32.0	41.0
24	36.91062414237598	39.0	35.0	40.0	31.0	41.0
25	36.99506246175962	39.0	35.0	40.0	31.0	41.0
26	36.88030746749197	39.0	35.0	40.0	31.0	41.0
27	36.86291084274369	39.0	35.0	40.0	31.0	41.0
28	36.762092869914405	39.0	35.0	40.0	31.0	41.0
29	36.61508586635619	39.0	35.0	40.0	31.0	41.0
30	36.59395757471353	39.0	35.0	40.0	31.0	41.0
31	36.496715634151705	38.0	35.0	40.0	30.0	41.0
32	36.42854885561027	38.0	35.0	40.0	30.0	41.0
33	36.33405131190486	38.0	35.0	40.0	30.0	41.0
34	36.235599935845265	38.0	35.0	40.0	30.0	41.0
35	36.122935910611076	38.0	35.0	40.0	30.0	41.0
36	36.05177999679226	38.0	35.0	40.0	30.0	41.0
37	35.97522558110524	38.0	35.0	40.0	30.0	41.0
38	35.871133340857654	38.0	35.0	40.0	29.0	41.0
39	35.80406432105879	38.0	35.0	40.0	29.0	41.0
40	35.631827875230925	38.0	34.0	40.0	28.0	41.0
41	35.518197964869344	38.0	34.0	40.0	28.0	41.0
42	35.41420552087108	38.0	34.0	40.0	27.0	41.0
43	35.3150662635215	38.0	34.0	40.0	27.0	41.0
44	35.209010175653276	38.0	34.0	40.0	26.0	41.0
45	35.05033295117706	38.0	34.0	40.0	26.0	41.0
46	34.85367968968119	37.0	33.0	40.0	25.0	41.0
47	34.76929720867515	37.0	33.0	40.0	25.0	41.0
48	34.63967970156169	37.0	33.0	40.0	24.0	41.0
49	34.39794110833239	37.0	33.0	40.0	24.0	41.0
50	34.34145167901249	37.0	33.0	40.0	24.0	41.0
51	33.20736472559002	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	4.0
10	4.0
11	8.0
12	8.0
13	14.0
14	15.0
15	43.0
16	106.0
17	235.0
18	449.0
19	902.0
20	1442.0
21	2346.0
22	3195.0
23	4217.0
24	5503.0
25	6439.0
26	7746.0
27	9579.0
28	11771.0
29	14953.0
30	18719.0
31	24664.0
32	31532.0
33	41627.0
34	58292.0
35	83517.0
36	92420.0
37	115820.0
38	157238.0
39	148870.0
40	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.698336135152633	33.433525599520024	20.345841525932173	25.522296739395163
2	21.247096701377544	33.29725619716887	20.159198778683994	25.29644832276958
3	19.98871351942166	34.70390809240658	20.503852254028978	24.803526134142793
4	19.0219967566219	35.832793760358314	19.23489542184706	25.910314061172723
5	17.540972894625853	38.151393286326126	16.928532816927344	27.37910100212067
6	17.95785984567223	43.47088979048728	17.897031655607893	20.674218708232598
7	83.9278140463221	9.331780947232733	3.56177566040762	3.1786293460375545
8	85.10160802646976	8.942931990044137	3.017886101590206	2.937573881895891
9	77.7770385463013	13.456336170794152	5.750877672371289	3.0157476105332566
10	27.651521001764255	47.95827566337775	11.406236077532181	12.983967257325817
11	18.38163749012433	33.0230541216445	31.80898522659095	16.786323161640222
12	23.32333390755778	28.139453377924834	30.930778232537143	17.606434481980244
13	22.95349375976429	30.225551403978777	27.854083626880833	18.966871209376094
14	18.55366721515002	33.747052149480524	27.62645313437446	20.07282750099499
15	17.25643477899289	36.31454827346548	27.342033823800215	19.08698312374141
16	19.96673458355857	33.8944892273513	26.528219171572324	19.610557017517806
17	18.42892190349465	33.194133406200436	28.73870609410549	19.638238596199425
18	19.13022816511527	34.27288333937259	26.8146581681448	19.78223032736734
19	18.43331769066727	36.83586487112621	26.608887806442798	18.12192963176372
20	19.53654146593562	35.47305204255598	27.153727805729964	17.83667868577844
21	19.728055220591294	35.61573691807797	26.53142690815775	18.124780953172987
22	19.023066002150372	33.72531082373487	25.3688006035297	21.882822570585056
23	18.147591524447108	36.77741278223627	24.993020202800235	20.081975490516385
24	16.888851927315066	36.79214460951747	27.319223252526093	18.99978021064137
25	19.43531955590669	36.08454167978473	24.175284983634604	20.30485378067398
26	19.129990554997832	36.40448370291607	23.529698294553384	20.935827447532716
27	17.75719810149516	35.49681305429985	25.19688968356273	21.54909916064226
28	18.85471923394498	35.24328305899265	27.780424490474804	18.12157321658756
29	18.33340263628425	35.276310865316646	24.838573626465017	21.551712871934086
30	20.09159870027266	34.94448833631336	25.975894453585834	18.988018509828148
31	19.51646341101204	35.61514289278438	24.117902140273134	20.750491555930452
32	20.989527334073884	35.11140944381412	23.17565921956957	20.723404002542427
33	19.17561169754608	34.23177678905568	25.69694017571268	20.89567133768556
34	17.457809353522272	34.21918345283142	26.43020499812882	21.892802195517486
35	19.10432866231444	33.35653992146986	26.40596876615006	21.13316265006564
36	20.601510012296323	34.98607010686515	25.332683865679	19.079736015159526
37	18.346471192743387	35.73608644256072	25.760500882127563	20.156941482568328
38	17.962730853079727	34.7665183583517	27.697617364547384	19.573133424021194
39	17.63447247583802	33.77295165228135	26.79493652839738	21.797639343483247
40	19.14068301028258	35.39689799991684	26.303202390357782	19.159216599442804
41	18.222438711440333	33.65010722156549	28.255644725352408	19.871809341641764
42	19.23727152302145	35.34094081726	24.59597369656	20.825813963158552
43	17.956077769791438	34.52261157280077	25.29466624688879	22.226644410519
44	20.13484374164652	32.5490219373541	25.8875034898986	21.42863083110079
45	19.908163689609903	33.46631579572658	26.920513475463785	19.705007039199728
46	18.4427032903061	34.679909470545255	26.825350623429543	20.052036615719096
47	18.826087214793606	35.5313853263872	25.943579477614158	19.698947981205038
48	18.863629613348937	33.56242908823058	27.311263313591894	20.262677984828596
49	18.780347267186638	34.386460975508335	25.910195256114005	20.922996501191022
50	18.014173443505225	31.865655239600105	27.533903993632048	22.586267323262625
51	18.081892326975282	32.37770504268072	27.99522403663948	21.54517859370452
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	24937.0
1	19044.5
2	13152.0
3	8165.0
4	3178.0
5	2912.0
6	2646.0
7	2831.5
8	3017.0
9	3937.0
10	4857.0
11	5773.5
12	6690.0
13	7193.5
14	7697.0
15	7758.5
16	7820.0
17	7690.0
18	7560.0
19	7439.0
20	7318.0
21	6955.0
22	6592.0
23	6763.5
24	6935.0
25	7401.0
26	9097.0
27	10327.0
28	12828.5
29	15330.0
30	18157.0
31	20984.0
32	22484.0
33	23984.0
34	29328.5
35	34673.0
36	35688.0
37	36703.0
38	38270.0
39	39837.0
40	37977.0
41	36117.0
42	40261.5
43	44406.0
44	46957.5
45	49509.0
46	57308.5
47	65108.0
48	69475.0
49	73842.0
50	70691.0
51	67540.0
52	60583.0
53	53626.0
54	46954.0
55	40282.0
56	36056.0
57	31830.0
58	27543.5
59	23257.0
60	20671.0
61	18085.0
62	16029.0
63	13973.0
64	12244.5
65	10516.0
66	8601.5
67	6687.0
68	5543.0
69	4399.0
70	3721.0
71	3043.0
72	3015.0
73	2987.0
74	2476.0
75	1445.0
76	925.0
77	762.0
78	599.0
79	463.0
80	327.0
81	314.0
82	301.0
83	242.0
84	183.0
85	122.5
86	62.0
87	40.0
88	18.0
89	15.5
90	13.0
91	11.0
92	9.0
93	5.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	841715.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.419989992581616
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.50629793443616	19.948756358046893
2	9.685505372303792	5.1178191001872335
3	3.755557279504899	2.976653572232594
4	1.9819272744657779	2.0944999502964166
5	1.242905169314685	1.6418771067510969
6	0.871245984085107	1.3810986108363268
7	0.6858806958357518	1.2684672784059836
8	0.5143184764851498	1.0870631201389986
9	0.44156681472014336	1.0499571743366096
>10	4.096119926542383	22.927004287354
>50	0.7225853701910959	13.331388298290642
>100	0.4873783302664591	21.708037824186665
>500	0.006418905572653271	1.0022616580676005
>1k	0.0013754797655685582	1.1777846387951751
>5k	4.5849325518951934E-4	0.6242028431257367
>10k+	4.5849325518951934E-4	2.6631281789480536
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21985	2.611929215946015	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5153	0.6122024675810696	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4083	0.48508105475131136	No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT	3485	0.41403562963710994	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2155	0.2560249015403076	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1880505871940027E-4	0.0	0.0	0.5063471602620839	0.0
2	1.1880505871940027E-4	0.0	0.0	0.698692550328793	0.0
3	1.1880505871940027E-4	0.0	0.0	0.9060073777941465	0.0
4	1.1880505871940027E-4	0.0	0.0	1.7854024224351472	0.0
5	1.1880505871940027E-4	0.0	0.0	1.9213154096101412	0.0
6	1.1880505871940027E-4	0.0	0.0	2.7908496343774316	0.0
7	1.1880505871940027E-4	0.0	0.0	3.426100283350065	0.0
8	1.1880505871940027E-4	0.0	0.0	4.02333331353249	0.0
9	1.1880505871940027E-4	0.0	0.0	4.917460185454697	0.0
10	1.1880505871940027E-4	0.0	0.0	6.269105338505313	0.0
11	1.1880505871940027E-4	0.0	0.0	7.394664464812911	0.0
12	1.1880505871940027E-4	0.0	0.0	7.930594084696127	0.0
13	1.1880505871940027E-4	0.0	0.0	8.168560617311085	0.0
14	1.1880505871940027E-4	0.0	0.0	8.398329600874405	0.0
15	1.1880505871940027E-4	0.0	0.0	8.614198392567555	0.0
16	1.1880505871940027E-4	0.0	0.0	9.050688178302632	0.0
17	1.1880505871940027E-4	0.0	0.0	9.660514544709313	0.0
18	1.1880505871940027E-4	0.0	0.0	10.38997760524643	0.0
19	1.1880505871940027E-4	0.0	0.0	10.735225105885009	0.0
20	1.1880505871940027E-4	0.0	0.0	11.099125000742532	0.0
21	1.1880505871940027E-4	0.0	0.0	11.496052701924048	0.0
22	2.3761011743880054E-4	0.0	0.0	11.963907023161045	0.0
23	2.3761011743880054E-4	0.0	0.0	12.443404240152546	0.0
24	2.3761011743880054E-4	0.0	0.0	12.78710727502777	0.0
25	2.3761011743880054E-4	0.0	0.0	13.088634514057608	0.0
26	2.3761011743880054E-4	0.0	0.0	13.36568791099125	0.0
27	2.3761011743880054E-4	0.0	0.0	13.663413388142066	0.0
28	2.3761011743880054E-4	0.0	0.0	13.986919563034993	0.0
29	2.3761011743880054E-4	0.0	0.0	14.306267560872742	0.0
30	2.3761011743880054E-4	0.0	0.0	14.716382623572112	0.0
31	3.5641517615820083E-4	0.0	0.0	15.04772993234052	0.0
32	3.5641517615820083E-4	0.0	0.0	15.36042484688998	0.0
33	3.5641517615820083E-4	0.0	0.0	15.682505361078274	0.0
34	3.5641517615820083E-4	0.0	0.0	15.994012225040542	0.0
35	3.5641517615820083E-4	0.0	0.0	16.35850614519166	0.0
36	3.5641517615820083E-4	0.0	0.0	16.70102112947969	0.0
37	3.5641517615820083E-4	0.0	0.0	17.030823972484747	0.0
38	3.5641517615820083E-4	0.0	0.0	17.366923483601933	0.0
39	3.5641517615820083E-4	0.0	0.0	17.67605424638981	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCCTA	30	2.1648066E-6	45.000004	6
CGTGCCG	30	2.1648066E-6	45.000004	1
CGACCGG	30	2.1648066E-6	45.000004	2
CGACATG	30	2.1648066E-6	45.000004	1
CCTGTCG	30	2.1648066E-6	45.000004	19
CCGTAGC	30	2.1648066E-6	45.000004	30
TACGAGC	30	2.1648066E-6	45.000004	24
CCATACG	30	2.1648066E-6	45.000004	1
ACGTCCA	30	2.1648066E-6	45.000004	13
TTACGTC	30	2.1648066E-6	45.000004	11
CGACTCG	30	2.1648066E-6	45.000004	1
TATCGAG	30	2.1648066E-6	45.000004	1
TTTTCGC	30	2.1648066E-6	45.000004	32
TACCGAG	30	2.1648066E-6	45.000004	1
CGGACGA	30	2.1648066E-6	45.000004	30
ATTTACG	50	2.1827873E-11	45.0	1
CGGCGTA	20	7.032209E-4	45.0	24
ACTATCG	25	3.8899125E-5	45.0	1
AACCGCG	45	3.8380676E-10	45.0	1
GGTACTA	35	1.2114106E-7	45.0	8
>>END_MODULE