FastQCFastQC Report
Sat 18 Jun 2016
SRR3553257_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553257_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831314
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA221372.6628927216430855No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50480.6072314432332429No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38800.4667309825168348No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT33100.3981648330233823No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20470.24623668072473215No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG8500.10224776678848185No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGACG351.2113924E-745.0000041
CGATTAG351.2113924E-745.00000442
CCGTCGT351.2113924E-745.00000441
CCCCGTC351.2113924E-745.00000411
GTATGCG351.2113924E-745.0000041
TTACGTC351.2113924E-745.00000411
TCGGTCA351.2113924E-745.00000434
GTGTTCG207.03216E-445.042
CGTGGAC253.8898703E-545.034
TCGCATA207.03216E-445.021
ATCTCCG406.8102963E-945.01
CGAGTAA453.8380676E-1045.030
GCGAATT207.03216E-445.018
CTATCGG207.03216E-445.02
GCGAAGT207.03216E-445.023
TCCGAGT207.03216E-445.017
GGGTCGA207.03216E-445.07
ACCGCCG253.8898703E-545.01
TAGCGTC253.8898703E-545.032
CGGCATA253.8898703E-545.035