##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553257_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	831314
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.483325193609154	31.0	31.0	34.0	30.0	34.0
2	31.699081213596788	31.0	31.0	34.0	30.0	34.0
3	31.88270015902535	33.0	31.0	34.0	30.0	34.0
4	35.6317083556875	37.0	35.0	37.0	33.0	37.0
5	35.38665414031281	37.0	35.0	37.0	33.0	37.0
6	32.57336096829838	37.0	35.0	37.0	26.0	37.0
7	34.20307609399096	37.0	35.0	37.0	28.0	37.0
8	35.31871591239892	37.0	35.0	37.0	32.0	37.0
9	37.166831065036796	39.0	37.0	39.0	34.0	39.0
10	36.55623867756347	38.0	35.0	39.0	32.0	39.0
11	36.63857339104117	39.0	35.0	39.0	32.0	39.0
12	36.708481993566814	39.0	35.0	39.0	32.0	39.0
13	36.70069913414185	39.0	35.0	39.0	32.0	39.0
14	37.83264085532061	39.0	37.0	41.0	33.0	41.0
15	37.858242493209545	40.0	37.0	41.0	33.0	41.0
16	37.7873041955266	39.0	37.0	41.0	33.0	41.0
17	37.77171201254881	39.0	37.0	41.0	33.0	41.0
18	37.62582008723539	39.0	37.0	41.0	33.0	41.0
19	37.544494619361636	39.0	36.0	41.0	32.0	41.0
20	37.4390723601431	39.0	36.0	41.0	32.0	41.0
21	37.387805329875356	39.0	36.0	40.0	32.0	41.0
22	37.315852974928845	39.0	36.0	40.0	32.0	41.0
23	37.16740846419042	39.0	35.0	40.0	32.0	41.0
24	37.02457434856144	39.0	35.0	40.0	31.0	41.0
25	37.065532398107095	39.0	35.0	40.0	31.0	41.0
26	36.96227779154447	39.0	35.0	40.0	31.0	41.0
27	36.907065200393596	39.0	35.0	40.0	31.0	41.0
28	36.83892488277594	39.0	35.0	40.0	31.0	41.0
29	36.65389130942099	39.0	35.0	40.0	31.0	41.0
30	36.6486850937191	39.0	35.0	40.0	31.0	41.0
31	36.248927601363626	38.0	35.0	40.0	30.0	41.0
32	36.35908453364192	38.0	35.0	40.0	30.0	41.0
33	36.31524309707283	38.0	35.0	40.0	30.0	41.0
34	36.26677765561509	38.0	35.0	40.0	30.0	41.0
35	36.25110367442387	38.0	35.0	40.0	30.0	41.0
36	36.15497272991914	38.0	35.0	40.0	30.0	41.0
37	36.05342024794482	38.0	35.0	40.0	30.0	41.0
38	35.94302634143055	38.0	35.0	40.0	30.0	41.0
39	35.8601767803742	38.0	35.0	40.0	29.0	41.0
40	35.710988868225485	38.0	35.0	40.0	28.0	41.0
41	35.662814532174366	38.0	35.0	40.0	28.0	41.0
42	35.546474617292624	38.0	34.0	40.0	28.0	41.0
43	35.483213322523135	38.0	34.0	40.0	28.0	41.0
44	35.311551351234314	38.0	34.0	40.0	27.0	41.0
45	35.141304007871874	38.0	34.0	40.0	26.0	41.0
46	34.837315382635204	37.0	33.0	40.0	25.0	41.0
47	34.826762210187724	37.0	33.0	40.0	25.0	41.0
48	34.815199792136305	37.0	33.0	40.0	26.0	41.0
49	34.63896193255497	37.0	33.0	40.0	26.0	41.0
50	34.5354799750756	37.0	33.0	40.0	25.0	41.0
51	33.45117849573086	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	3.0
10	4.0
11	6.0
12	6.0
13	4.0
14	19.0
15	38.0
16	85.0
17	225.0
18	447.0
19	901.0
20	1482.0
21	2219.0
22	3093.0
23	4157.0
24	5241.0
25	6354.0
26	7328.0
27	8985.0
28	11187.0
29	14393.0
30	18152.0
31	23900.0
32	31309.0
33	41741.0
34	58486.0
35	83960.0
36	93314.0
37	117775.0
38	153010.0
39	143461.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.548192379774672	33.433696533439836	20.409375999923014	25.608735086862485
2	21.31781733496609	33.25554483624719	20.108887857055215	25.3177499717315
3	20.067267001397788	34.629033072942356	20.50067724109061	24.80302268456925
4	19.00040177357773	35.96559182210332	19.167967819620504	25.86603858469844
5	17.567730123635595	38.21504269144992	16.835154947468705	27.382072237445776
6	17.15019836066757	45.858243696124454	17.092578736795	19.89897920641298
7	83.9243655225342	9.388269655028065	3.519488424349885	3.167876398087847
8	84.92759655196473	9.057347765104401	2.991168198779282	3.023887484151596
9	77.71179121246605	13.563226410237286	5.756789853172207	2.9681925241244587
10	27.546390413249387	48.06222438212276	11.427691582242089	12.963693622385767
11	18.341926155459912	33.0650031155496	31.789913317952063	16.803157411038427
12	23.268343850819306	28.125353356252873	31.026062354296936	17.580240438630888
13	22.839865562230397	30.32740937840575	27.918692575849803	18.914032483514053
14	18.636760598281757	33.71481774636298	27.522091532200825	20.12633012315443
15	17.145266409563657	36.438457670627464	27.333715058329343	19.082560861479536
16	19.922315755538822	33.85255150280159	26.61954448018438	19.60558826147521
17	18.366225036508467	33.16207834825349	28.77709265091169	19.694603964326355
18	19.041180588802785	34.35585109838159	26.973802919233886	19.629165393581726
19	18.3913659579894	36.89147542324561	26.610883492879942	18.106275125885045
20	19.486018520077852	35.55720221240109	27.083027592462056	17.873751675059
21	19.76292953083913	35.65764560683448	26.508274851620445	18.071150010705942
22	18.996672737377214	33.78566943417289	25.37885804882391	21.83879977962599
23	18.082096536326826	36.98963327936255	24.98875274565327	19.939517438657354
24	16.91166033532456	36.848531361194446	27.37196775225727	18.867840551223725
25	19.45666739643504	36.233841845560164	24.043983380527695	20.265507377477103
26	19.108664114883183	36.46023043037889	23.50616012722028	20.92494532751764
27	17.772586531683576	35.64537587481986	25.171355227988464	21.410682365508098
28	18.82200949340442	35.4488195796053	27.74884099149058	17.980329935499704
29	18.19589228618789	35.47143437978911	24.870025044688287	21.462648289334716
30	20.06654525245575	35.10165833848582	25.935085900153254	18.89671050890518
31	19.608475257243352	35.63623372155407	23.949554560611272	20.805736460591305
32	20.965844434233034	35.2781259548137	23.123152021979664	20.6328775889736
33	19.197318943263316	34.316876655511635	25.690052134331914	20.795752266893135
34	17.379834815725467	34.298832931960725	26.47230769600897	21.849024556304837
35	19.078711533788674	33.49059440837036	26.37908179099594	21.05161226684502
36	20.69615091289212	35.16384903899129	25.193488862210913	18.94651118590569
37	18.32015339570848	35.92060280471639	25.666595293715734	20.092648505859398
38	17.832251110891914	34.944557652102574	27.719128993376753	19.504062243628763
39	17.519733818990176	33.884549039231864	26.85712017360468	21.738596968173276
40	19.074020165665438	35.53627149308204	26.3358971459641	19.05381119528842
41	18.24581325467874	33.66345327998806	28.272830723408966	19.817902741924232
42	19.09952196161739	35.61530300223502	24.520698556742698	20.764476479404895
43	17.95278318421198	34.72827355247235	25.181219130196293	22.137724133119374
44	20.087716554755485	32.761026519461964	25.90717827439451	21.244078651388044
45	19.824759356873574	33.690879739785444	26.781336534690865	19.703024368650112
46	18.4548798648886	34.79539620408173	26.754872406816197	19.994851524213473
47	18.913431026062355	35.52508438448047	25.93532648313393	19.626158106323246
48	18.824174740230525	33.83258311540525	27.151232867484488	20.192009276879734
49	18.846188082962634	34.55168564465412	25.854370310135522	20.74775596224772
50	17.95278318421198	32.01485840488672	27.628790084131865	22.403568326769427
51	18.073074674551375	32.53223210483644	27.963681593236732	21.431011627375458
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	24979.0
1	18948.5
2	12918.0
3	8068.5
4	3219.0
5	2945.0
6	2671.0
7	2793.5
8	2916.0
9	3844.5
10	4773.0
11	5729.5
12	6686.0
13	7158.0
14	7630.0
15	7711.5
16	7793.0
17	7606.0
18	7419.0
19	7442.0
20	7465.0
21	6961.0
22	6457.0
23	6727.5
24	6998.0
25	7534.5
26	9185.5
27	10300.0
28	12746.5
29	15193.0
30	17975.5
31	20758.0
32	22287.0
33	23816.0
34	29416.5
35	35017.0
36	35703.0
37	36389.0
38	38019.0
39	39649.0
40	38008.0
41	36367.0
42	40188.0
43	44009.0
44	46623.0
45	49237.0
46	56530.0
47	63823.0
48	68212.0
49	72601.0
50	69588.0
51	66575.0
52	59483.5
53	52392.0
54	45767.0
55	39142.0
56	35122.5
57	31103.0
58	26848.0
59	22593.0
60	20146.5
61	17700.0
62	15638.0
63	13576.0
64	11909.5
65	10243.0
66	8337.5
67	6432.0
68	5326.0
69	4220.0
70	3632.0
71	3044.0
72	2972.0
73	2900.0
74	2390.0
75	1399.0
76	918.0
77	749.5
78	581.0
79	445.5
80	310.0
81	293.5
82	277.0
83	228.5
84	180.0
85	120.0
86	60.0
87	38.5
88	17.0
89	12.0
90	7.0
91	6.0
92	5.0
93	4.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	831314.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.886120990187262
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.62483059246856	20.063722241813373
2	9.971386998196667	5.3618383454699154
3	3.8782580338253316	3.1281394418578095
4	2.0906325379267674	2.248359974428853
5	1.3438625817730125	1.806562598386732
6	0.9366511608403312	1.5109749861571498
7	0.7104860305640525	1.33715493657081
8	0.5932674429390629	1.2760528200318928
9	0.47308651726849493	1.1447515207896364
>10	4.2309336968689975	23.63102058892635
>50	0.6874203009749758	12.928332323597013
>100	0.45195286058439976	20.329299819921225
>500	0.0049714814664283975	0.8080587428869715
>1k	0.0013558585817531994	1.1224118207589409
>5k	4.519528605843998E-4	0.6133955690366064
>10k+	4.519528605843998E-4	2.6899242693667507
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22137	2.6628927216430855	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5048	0.6072314432332429	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3880	0.4667309825168348	No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT	3310	0.3981648330233823	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2047	0.24623668072473215	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	850	0.10224776678848185	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.405829806787808E-4	0.0	0.0	0.4782789655894163	0.0
2	2.405829806787808E-4	0.0	0.0	0.6796469204175558	0.0
3	2.405829806787808E-4	0.0	0.0	0.8752408837094047	0.0
4	2.405829806787808E-4	0.0	0.0	1.6848026136935021	0.0
5	2.405829806787808E-4	0.0	0.0	1.8106275125885045	0.0
6	2.405829806787808E-4	0.0	0.0	2.6530288194352556	0.0
7	2.405829806787808E-4	0.0	0.0	3.2890099288596124	0.0
8	2.405829806787808E-4	0.0	0.0	3.8723033655153167	0.0
9	2.405829806787808E-4	0.0	0.0	4.760174855710357	0.0
10	2.405829806787808E-4	0.0	0.0	6.08374212391467	0.0
11	2.405829806787808E-4	0.0	0.0	7.175267107254299	0.0
12	2.405829806787808E-4	0.0	0.0	7.71369181801341	0.0
13	2.405829806787808E-4	0.0	0.0	7.944892062445718	0.0
14	2.405829806787808E-4	0.0	0.0	8.162499368469676	0.0
15	2.405829806787808E-4	0.0	0.0	8.37252831060225	0.0
16	2.405829806787808E-4	0.0	0.0	8.793789109770797	0.0
17	2.405829806787808E-4	0.0	0.0	9.388510238008744	0.0
18	2.405829806787808E-4	0.0	0.0	10.110379471535424	0.0
19	2.405829806787808E-4	0.0	0.0	10.452247887079972	0.0
20	2.405829806787808E-4	0.0	0.0	10.790988723875696	0.0
21	2.405829806787808E-4	0.0	0.0	11.186266561130932	0.0
22	4.811659613575616E-4	0.0	0.0	11.634352362645162	0.0
23	4.811659613575616E-4	0.0	0.0	12.11022549842779	0.0
24	4.811659613575616E-4	0.0	0.0	12.448966335223513	0.0
25	4.811659613575616E-4	0.0	0.0	12.732974543914814	0.0
26	4.811659613575616E-4	0.0	0.0	13.008081182321	0.0
27	4.811659613575616E-4	0.0	0.0	13.290164727166871	0.0
28	4.811659613575616E-4	0.0	0.0	13.599554440319784	0.0
29	4.811659613575616E-4	0.0	0.0	13.923379132313421	0.0
30	4.811659613575616E-4	0.0	0.0	14.325513584518005	0.0
31	6.014574516969521E-4	0.0	0.0	14.64609040627248	0.0
32	6.014574516969521E-4	0.0	0.0	14.94958583639876	0.0
33	6.014574516969521E-4	0.0	0.0	15.275816358199188	0.0
34	6.014574516969521E-4	0.0	0.0	15.586408986255494	0.0
35	6.014574516969521E-4	0.0	0.0	15.96015464673998	0.0
36	7.217489420363425E-4	0.0	0.0	16.28987362176025	0.0
37	8.420404323757328E-4	0.0	0.0	16.618269390386786	0.0
38	8.420404323757328E-4	0.0	0.0	16.950153612233162	0.0
39	8.420404323757328E-4	0.0	0.0	17.26652023182576	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGACG	35	1.2113924E-7	45.000004	1
CGATTAG	35	1.2113924E-7	45.000004	42
CCGTCGT	35	1.2113924E-7	45.000004	41
CCCCGTC	35	1.2113924E-7	45.000004	11
GTATGCG	35	1.2113924E-7	45.000004	1
TTACGTC	35	1.2113924E-7	45.000004	11
TCGGTCA	35	1.2113924E-7	45.000004	34
GTGTTCG	20	7.03216E-4	45.0	42
CGTGGAC	25	3.8898703E-5	45.0	34
TCGCATA	20	7.03216E-4	45.0	21
ATCTCCG	40	6.8102963E-9	45.0	1
CGAGTAA	45	3.8380676E-10	45.0	30
GCGAATT	20	7.03216E-4	45.0	18
CTATCGG	20	7.03216E-4	45.0	2
GCGAAGT	20	7.03216E-4	45.0	23
TCCGAGT	20	7.03216E-4	45.0	17
GGGTCGA	20	7.03216E-4	45.0	7
ACCGCCG	25	3.8898703E-5	45.0	1
TAGCGTC	25	3.8898703E-5	45.0	32
CGGCATA	25	3.8898703E-5	45.0	35
>>END_MODULE