##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553256_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	663039
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.642639422417083	31.0	31.0	34.0	30.0	34.0
2	31.834088190890732	33.0	31.0	34.0	30.0	34.0
3	32.00852287723648	33.0	31.0	34.0	30.0	34.0
4	35.72987863459012	37.0	35.0	37.0	33.0	37.0
5	35.46994369863613	37.0	35.0	37.0	33.0	37.0
6	34.10249020042561	37.0	35.0	37.0	32.0	37.0
7	34.892786095538874	37.0	35.0	37.0	32.0	37.0
8	35.567455308058804	37.0	35.0	37.0	32.0	37.0
9	37.25373620556257	39.0	37.0	39.0	34.0	39.0
10	36.719628860444104	39.0	37.0	39.0	32.0	39.0
11	36.725998018216124	39.0	37.0	39.0	32.0	39.0
12	36.6245198246257	39.0	35.0	39.0	32.0	39.0
13	36.58577851378275	39.0	35.0	39.0	32.0	39.0
14	37.74080710184469	40.0	37.0	41.0	33.0	41.0
15	37.739359223213114	40.0	37.0	41.0	33.0	41.0
16	37.6653741333466	39.0	36.0	41.0	32.0	41.0
17	37.600643401066904	39.0	36.0	41.0	32.0	41.0
18	37.477469651106496	39.0	36.0	41.0	33.0	41.0
19	37.364269673427955	39.0	35.0	41.0	32.0	41.0
20	37.24071585532676	39.0	35.0	41.0	32.0	41.0
21	37.196418310235146	39.0	35.0	40.0	32.0	41.0
22	37.15940087988791	39.0	35.0	40.0	32.0	41.0
23	36.96919034928564	39.0	35.0	40.0	31.0	41.0
24	36.7787188988883	39.0	35.0	40.0	31.0	41.0
25	36.83752991905453	39.0	35.0	40.0	31.0	41.0
26	36.72477938703455	39.0	35.0	40.0	31.0	41.0
27	36.6585283821917	39.0	35.0	40.0	31.0	41.0
28	36.508504024650136	39.0	35.0	40.0	30.0	41.0
29	36.307185550171255	38.0	35.0	40.0	30.0	41.0
30	36.32379844926166	38.0	35.0	40.0	30.0	41.0
31	36.2382378713771	38.0	35.0	40.0	30.0	41.0
32	36.096688128450964	38.0	35.0	40.0	30.0	41.0
33	35.97379490497542	38.0	35.0	40.0	29.0	41.0
34	35.85415940842092	38.0	35.0	40.0	29.0	41.0
35	35.69424573818433	38.0	35.0	40.0	28.0	41.0
36	35.56788514702755	38.0	34.0	40.0	28.0	41.0
37	35.509980559212956	38.0	34.0	40.0	27.0	41.0
38	35.392470126191675	38.0	34.0	40.0	27.0	41.0
39	35.33333634974715	38.0	34.0	40.0	27.0	41.0
40	35.1721497528803	38.0	34.0	40.0	26.0	41.0
41	35.03778812407717	38.0	34.0	40.0	26.0	41.0
42	34.93573077903412	38.0	34.0	40.0	26.0	41.0
43	34.831795716390744	37.0	34.0	40.0	25.0	41.0
44	34.67115056580382	37.0	33.0	40.0	24.0	41.0
45	34.446994822325685	37.0	33.0	40.0	24.0	41.0
46	34.26516238109674	37.0	33.0	40.0	23.0	41.0
47	34.14768814504124	36.0	33.0	40.0	23.0	41.0
48	33.98594652803229	36.0	33.0	40.0	23.0	41.0
49	33.793613950310615	36.0	33.0	40.0	23.0	41.0
50	33.72985902790032	36.0	33.0	40.0	23.0	41.0
51	32.505415216902776	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	3.0
11	17.0
12	19.0
13	22.0
14	37.0
15	76.0
16	157.0
17	295.0
18	585.0
19	1121.0
20	1711.0
21	2399.0
22	3399.0
23	4332.0
24	5560.0
25	6362.0
26	7355.0
27	8518.0
28	10217.0
29	12686.0
30	15850.0
31	19360.0
32	25157.0
33	33221.0
34	48745.0
35	66919.0
36	66722.0
37	88370.0
38	118574.0
39	115231.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.99463379982173	31.8299526875493	19.964888943184338	26.210524569444633
2	23.69981252988135	33.90403882727864	19.059512336378404	23.33663630646161
3	21.009171406206875	33.67298152899	19.232503668713303	26.085343396089822
4	20.595017789300478	34.832641820466065	18.808999168977994	25.76334122125546
5	17.677391525988668	38.6544381250575	15.400300736457433	28.267869612496398
6	17.558394000956206	44.26164976720827	17.916593141579906	20.263363090255627
7	81.76985064226992	11.022428544927221	3.9358167468278635	3.271904065975003
8	83.05695441746262	10.707967404632306	3.250035065810608	2.985043112094462
9	77.17012121458919	13.45214987353685	5.553067014157538	3.824661897716424
10	35.17063098852405	36.18625752029669	12.481166266237732	16.16194522494152
11	25.20801943777063	33.0912661246171	22.923387613699948	18.77732682391232
12	25.4930705433617	29.07792754272373	26.353804225694116	19.075197688220452
13	24.90396492514015	29.36765408972926	25.095808843823665	20.63257214130692
14	21.031794509825215	31.424094208636294	26.061513726945172	21.48259755459332
15	18.598151843255074	33.46545225846443	27.30095816384858	20.63543773443191
16	20.39110821535385	30.62006910604052	26.66675715908114	22.322065519524493
17	20.768159942326168	31.70492233488528	26.660724331449583	20.86619339133897
18	21.620900128046767	31.058957316236302	25.18509469277071	22.135047862946223
19	20.909629750286182	33.23635562915605	24.315915051754118	21.538099568803645
20	22.522657038273767	31.305850787057775	25.448729260269758	20.7227629143987
21	22.89669235143031	31.708843672845788	24.41952886632611	20.974935109397787
22	21.462387582027603	30.760332348474222	23.987125945834258	23.790154123663918
23	21.823753957157873	32.697624121658	24.061782187774778	21.416839733409347
24	19.2135002616739	31.96463556442381	26.646999648587787	22.1748645253145
25	22.11468706968972	31.443851719129647	23.859531641426823	22.581929569753814
26	21.831898274460475	33.18432249083387	22.747681508930846	22.236097725774805
27	21.19377593173252	31.88877275695698	24.750731103298598	22.1667202080119
28	21.419102043771183	32.169450062515175	26.421371895167557	19.99007599854609
29	21.408695416106745	33.79876598510796	23.188077926034516	21.604460672750772
30	21.65392985932954	33.31010694695184	24.488001459944286	20.547961733774333
31	23.03861462146269	32.311372332547556	22.484650224194958	22.165362821794794
32	23.216432215902834	32.20745687659399	23.11704138067293	21.459069526830245
33	22.04500791054523	30.882044645940887	23.458650245309855	23.614297198204028
34	21.003591040647684	32.151502400311294	25.045133091718586	21.799773467322435
35	22.76955050909524	31.490606133274213	24.826292269383853	20.913551088246695
36	22.229009153307725	33.42714380300404	23.821072365275647	20.52277467841258
37	22.046365296762332	33.17859130458389	23.232117567744883	21.542925830908892
38	20.918980633115094	32.302775553172594	25.769826510959387	21.008417302752928
39	20.964076019660986	30.167757854364524	25.063834857376417	23.804331268598077
40	22.20698933245254	29.80427998956321	25.245724610467864	22.74300606751639
41	21.188044745482543	29.556179953215423	26.808679429113518	22.447095872188513
42	22.121624821466007	30.25975847574577	24.35950223139212	23.259114471396103
43	20.79681587357606	29.43959555923558	26.09831397549767	23.665274591690686
44	22.766081633207097	27.508789075755725	26.65966858661406	23.065460704423117
45	22.784029295410978	28.916700224270368	26.271154487141786	22.028115993176872
46	20.79063222525372	30.070176867424088	26.723918200890147	22.41527270643205
47	21.63462481090856	30.315562131337675	25.2724198727375	22.777393185016265
48	20.87599673624025	29.99159928752306	26.71290829046255	22.41949568577414
49	21.531312637718145	28.912024782855912	26.380801129345333	23.175861450080614
50	21.242189373475767	27.64588508368286	26.487883819805468	24.6240417230359
51	20.611004782524105	27.703046125491866	27.353292943552336	24.332656148431692
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	20799.0
1	15972.0
2	11145.0
3	6849.5
4	2554.0
5	2135.5
6	1717.0
7	1889.5
8	2062.0
9	2255.0
10	2448.0
11	2488.5
12	2529.0
13	2687.0
14	2845.0
15	2911.0
16	2977.0
17	2949.5
18	2922.0
19	2875.0
20	2828.0
21	2904.0
22	2980.0
23	3451.5
24	3923.0
25	4545.5
26	6454.0
27	7740.0
28	9262.5
29	10785.0
30	10935.5
31	11086.0
32	15932.5
33	20779.0
34	18313.0
35	15847.0
36	17948.0
37	20049.0
38	22249.0
39	24449.0
40	25729.0
41	27009.0
42	26512.0
43	26015.0
44	29498.0
45	32981.0
46	36425.0
47	39869.0
48	44033.5
49	48198.0
50	47846.0
51	47494.0
52	45359.0
53	43224.0
54	39437.0
55	35650.0
56	33699.5
57	31749.0
58	30478.5
59	29208.0
60	29116.5
61	29025.0
62	25050.0
63	21075.0
64	20645.0
65	20215.0
66	16906.5
67	13598.0
68	11986.0
69	10374.0
70	9683.5
71	8993.0
72	8074.5
73	7156.0
74	5917.5
75	4783.5
76	4888.0
77	3255.5
78	1623.0
79	1314.0
80	1005.0
81	796.5
82	588.0
83	440.5
84	293.0
85	278.0
86	263.0
87	218.0
88	173.0
89	107.0
90	41.0
91	28.5
92	16.0
93	9.5
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	663039.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.39551894859395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.55163393969454	18.37756240279139
2	9.060579426971433	4.239539153379013
3	3.3430654807836233	2.3463825540619077
4	1.6848607857769549	1.5767276975755058
5	1.0211119158718764	1.1944721588207778
6	0.7071490130228558	0.9926470880193441
7	0.4949164559528191	0.8105179126250599
8	0.40568877217748645	0.7593039485368157
9	0.3071858221917137	0.6468094551443085
>10	2.64780561956712	13.889902085760466
>50	0.8847486316724446	15.074310646483099
>100	0.8795442279567242	34.16471364708259
>500	0.007806605573580392	1.1089267022432265
>1k	0.0032527523223251633	2.049368383983673
>5k	0.0	0.0
>10k+	6.505504644650328E-4	2.768816163492832
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18192	2.7437300068321773	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4621	0.6969424121356361	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3483	0.5253084660178361	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1930	0.29108393322263093	No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT	1893	0.2855035676634406	No Hit
GCCCCTGGGCTATATCTTTTATTTTGTTTTGGTTTAATATAACAGATAACC	1538	0.23196222243337117	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	870	0.13121400098636732	No Hit
CTACTTGGGGACTCCTAGGACTTTGATCTTTTAAAGGTTCCCTCCCCCATC	863	0.1301582561508448	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5082069078892795E-4	0.0	0.0	0.3640811475644721	0.0
2	1.5082069078892795E-4	0.0	0.0	0.4768950242745902	0.0
3	1.5082069078892795E-4	0.0	0.0	0.615348418418826	0.0
4	1.5082069078892795E-4	0.0	0.0	1.2177262574298042	0.0
5	1.5082069078892795E-4	0.0	0.0	1.325110589271521	0.0
6	1.5082069078892795E-4	0.0	0.0	1.9878167045980704	0.0
7	1.5082069078892795E-4	0.0	0.0	2.476777384135775	0.0
8	1.5082069078892795E-4	0.0	0.0	2.924564015088102	0.0
9	1.5082069078892795E-4	0.0	0.0	3.515931943671488	0.0
10	1.5082069078892795E-4	0.0	0.0	4.482089288865361	0.0
11	1.5082069078892795E-4	0.0	0.0	5.347950874684596	0.0
12	1.5082069078892795E-4	0.0	0.0	5.788196471097477	0.0
13	1.5082069078892795E-4	0.0	0.0	5.964958320702101	0.0
14	1.5082069078892795E-4	0.0	0.0	6.151071053135637	0.0
15	1.5082069078892795E-4	0.0	0.0	6.32089515096397	0.0
16	1.5082069078892795E-4	0.0	0.0	6.66009088454827	0.0
17	1.5082069078892795E-4	0.0	0.0	7.0777133773428105	0.0
18	1.5082069078892795E-4	0.0	0.0	7.633336802209222	0.0
19	1.5082069078892795E-4	0.0	0.0	7.849161210728178	0.0
20	1.5082069078892795E-4	0.0	0.0	8.082782460760226	0.0
21	1.5082069078892795E-4	0.0	0.0	8.326659517765924	0.0
22	1.5082069078892795E-4	0.0	0.0	8.596025271514948	0.0
23	1.5082069078892795E-4	0.0	0.0	8.873535342566576	0.0
24	1.5082069078892795E-4	0.0	0.0	9.07970722687504	0.0
25	1.5082069078892795E-4	0.0	0.0	9.270947862795401	0.0
26	1.5082069078892795E-4	0.0	0.0	9.429611229505353	0.0
27	1.5082069078892795E-4	0.0	0.0	9.626130589603326	0.0
28	1.5082069078892795E-4	0.0	0.0	9.833207398056524	0.0
29	1.5082069078892795E-4	0.0	0.0	10.032893992661066	0.0
30	1.5082069078892795E-4	0.0	0.0	10.274810380686505	0.0
31	1.5082069078892795E-4	0.0	0.0	10.465749375225288	0.0
32	1.5082069078892795E-4	0.0	0.0	10.662117914632473	0.0
33	1.5082069078892795E-4	0.0	0.0	10.862558612690957	0.0
34	1.5082069078892795E-4	0.0	0.0	11.06480915903891	0.0
35	1.5082069078892795E-4	0.0	0.0	11.286817215880212	0.0
36	1.5082069078892795E-4	0.0	0.0	11.483638217359763	0.0
37	1.5082069078892795E-4	0.0	0.0	11.683475632655092	0.0
38	1.5082069078892795E-4	0.0	0.0	11.886781923838567	0.0
39	1.5082069078892795E-4	0.0	0.0	12.136239346403455	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATAAGG	105	0.0	45.000004	2
TCACGGA	20	7.031125E-4	45.0	23
CCTTCGT	20	7.031125E-4	45.0	20
AGCGTTG	35	1.210883E-7	45.0	1
TAGAGTA	35	1.210883E-7	45.0	19
AGCGTAA	35	1.210883E-7	45.0	10
CGTGGAT	25	3.889014E-5	45.0	38
CGGAAAT	25	3.889014E-5	45.0	14
ACTATCG	35	1.210883E-7	45.0	1
AGTCCGT	40	6.8066583E-9	45.0	15
ACAGTCG	25	3.889014E-5	45.0	17
TACCCGT	25	3.889014E-5	45.0	37
TCGCAAT	20	7.031125E-4	45.0	10
CGAACGA	25	3.889014E-5	45.0	13
GATCGTA	20	7.031125E-4	45.0	20
ATTAGGC	20	7.031125E-4	45.0	41
GATCGCC	25	3.889014E-5	45.0	29
CAACGTA	25	3.889014E-5	45.0	17
ACTGATC	45	3.8380676E-10	45.0	11
CGAAACG	25	3.889014E-5	45.0	1
>>END_MODULE