FastQCFastQC Report
Sat 18 Jun 2016
SRR3553255_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553255_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658742
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180942.746750624675518No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45970.6978452869256856No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34790.5281278558221579No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT19450.29525975268010846No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17890.2715782506656627No Hit
GCCCCTGGGCTATATCTTTTATTTTGTTTTGGTTTAATATAACAGATAACC13580.20615051112575183No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG8950.13586502758287766No Hit
CTACTTGGGGACTCCTAGGACTTTGATCTTTTAAAGGTTCCCTCCCCCATC8200.12447969007593261No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGAAC207.031092E-445.038
GTCATAT253.8889855E-545.035
GACAGTA207.031092E-445.013
TCGTTTA253.8889855E-545.045
ACAGTCG253.8889855E-545.017
CAAACGT253.8889855E-545.039
GTACATA207.031092E-445.010
GTCGAGT207.031092E-445.024
CTCCGAT253.8889855E-545.018
CGAGTAC207.031092E-445.036
TTAGCGA253.8889855E-545.012
TGGACGG502.1827873E-1145.02
GTTTAGC351.2108649E-745.043
GTTTACG207.031092E-445.01
CGCATTA253.8889855E-545.022
TTTCGAA207.031092E-445.029
TGGGTAT700.045.06
TAGCGAG207.031092E-445.01
ATTACAC351.2108649E-745.019
TTGTCGA207.031092E-445.022