##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553255_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	658742
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.625428468201513	31.0	31.0	34.0	30.0	34.0
2	31.81968965088001	33.0	31.0	34.0	30.0	34.0
3	31.991239362299655	33.0	31.0	34.0	30.0	34.0
4	35.72364901585143	37.0	35.0	37.0	33.0	37.0
5	35.48981543608879	37.0	35.0	37.0	33.0	37.0
6	32.65572409228499	37.0	35.0	37.0	26.0	37.0
7	34.17720594709309	37.0	35.0	37.0	27.0	37.0
8	35.265367928566874	37.0	35.0	37.0	32.0	37.0
9	37.128613326613454	39.0	37.0	39.0	34.0	39.0
10	36.67181992342981	38.0	37.0	39.0	32.0	39.0
11	36.71808082678803	39.0	37.0	39.0	32.0	39.0
12	36.64096414074099	39.0	35.0	39.0	32.0	39.0
13	36.62324551949018	39.0	35.0	39.0	32.0	39.0
14	37.745162749604546	40.0	37.0	41.0	33.0	41.0
15	37.79009688163196	40.0	37.0	41.0	33.0	41.0
16	37.74339119108847	40.0	37.0	41.0	33.0	41.0
17	37.69873334325123	39.0	36.0	41.0	33.0	41.0
18	37.5613077654074	39.0	36.0	41.0	33.0	41.0
19	37.46006023602564	39.0	35.0	41.0	32.0	41.0
20	37.33817184876629	39.0	35.0	41.0	32.0	41.0
21	37.275626876683134	39.0	35.0	41.0	32.0	41.0
22	37.20703553136129	39.0	35.0	41.0	32.0	41.0
23	37.030401887233545	39.0	35.0	40.0	31.0	41.0
24	36.89116072756861	39.0	35.0	40.0	31.0	41.0
25	36.920890120866744	39.0	35.0	40.0	31.0	41.0
26	36.80465493319084	39.0	35.0	40.0	31.0	41.0
27	36.70620516074578	39.0	35.0	40.0	31.0	41.0
28	36.589502415209594	39.0	35.0	40.0	31.0	41.0
29	36.35446047162622	38.0	35.0	40.0	30.0	41.0
30	36.38227105604319	38.0	35.0	40.0	30.0	41.0
31	35.98528103567102	38.0	35.0	40.0	29.0	41.0
32	36.04089157818994	38.0	35.0	40.0	30.0	41.0
33	35.961727049436654	38.0	35.0	40.0	29.0	41.0
34	35.87927595325636	38.0	35.0	40.0	29.0	41.0
35	35.81697994055336	38.0	35.0	40.0	29.0	41.0
36	35.66184181363872	38.0	35.0	40.0	28.0	41.0
37	35.58332852619083	38.0	34.0	40.0	28.0	41.0
38	35.45927692480516	38.0	34.0	40.0	27.0	41.0
39	35.382208816198144	38.0	34.0	40.0	27.0	41.0
40	35.257202364506895	38.0	34.0	40.0	26.0	41.0
41	35.19337312635296	38.0	34.0	40.0	26.0	41.0
42	35.08400557426124	38.0	34.0	40.0	26.0	41.0
43	34.98341232227488	38.0	34.0	40.0	26.0	41.0
44	34.78124667927656	37.0	34.0	40.0	25.0	41.0
45	34.554553983198275	37.0	33.0	40.0	24.0	41.0
46	34.27736959234419	37.0	33.0	40.0	23.0	41.0
47	34.21191452799427	36.0	33.0	40.0	23.0	41.0
48	34.165023635960665	36.0	33.0	40.0	23.0	41.0
49	34.00085617738052	36.0	33.0	40.0	23.0	41.0
50	33.91258489666668	36.0	33.0	40.0	23.0	41.0
51	32.75263912123411	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	9.0
11	12.0
12	14.0
13	33.0
14	33.0
15	67.0
16	160.0
17	329.0
18	598.0
19	998.0
20	1654.0
21	2406.0
22	3282.0
23	4229.0
24	5291.0
25	6253.0
26	6843.0
27	8455.0
28	10060.0
29	12367.0
30	15340.0
31	19490.0
32	24878.0
33	33386.0
34	48526.0
35	66997.0
36	68493.0
37	90749.0
38	116811.0
39	110960.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.930740714877754	31.811847430405226	19.9396728916632	26.31773896305382
2	23.708219606462013	33.69042811905116	19.19127670620668	23.410075568280146
3	20.97103266529233	33.58401316448625	19.2134401632202	26.231514007001223
4	20.58514562605694	34.67078158064918	18.901026502029627	25.84304629126426
5	17.622832611249926	38.54604685901309	15.454760740927403	28.37635978880958
6	16.757243351721918	46.57210258340898	17.265940231532223	19.404713833336874
7	81.85845141193366	10.920208518661328	3.943880912405767	3.2774591569992504
8	82.90225915457037	10.688251242519833	3.2823169010022135	3.1271727019075755
9	77.27471453163758	13.320844883125716	5.599460790415671	3.8049797948210373
10	35.18236274596124	36.07983095050869	12.607819146190769	16.129987157339293
11	25.29973798543284	32.9798312541177	22.904718387471878	18.815712372977583
12	25.595756760613416	28.873671331112938	26.548785412194757	18.98178649607889
13	24.897304255687356	29.24953927334222	25.16068506334802	20.692471407622406
14	21.094601528367708	31.283871379083163	26.15075401295196	21.470773079597173
15	18.606829380850165	33.49308226893078	27.30644167215693	20.593646678062125
16	20.462943003482394	30.493121738100804	26.73383509780764	22.31010016060916
17	20.66347674810472	31.599321130275587	26.70408141578949	21.033120705830203
18	21.59085043917042	31.014873804919073	25.238105358395245	22.156170397515265
19	20.949324621779088	33.284351081303456	24.324090463337694	21.442233833579763
20	22.599135928785476	31.269601756074454	25.54945638808517	20.5818059270549
21	22.90122688396975	31.602053611277253	24.463598798922796	21.033120705830203
22	21.47881871810208	30.63930947168998	24.027312665656662	23.85455914455128
23	21.83267500781793	32.65588045092009	24.08682002969296	21.42462451156902
24	19.19886693121131	31.972001178002923	26.615123978735227	22.214007912050544
25	22.112906114988874	31.299203633592516	23.913003877086933	22.67488637433168
26	21.792902228793672	33.124045529205674	22.93037334798753	22.152678894013135
27	21.19388167142827	31.949534111989212	24.741249229592164	22.115334986990355
28	21.453315562086523	32.11636725759098	26.50157421266596	19.928742967656532
29	21.468040598595508	33.76420510609617	23.183127840641706	21.584626454666623
30	21.66326118571459	33.29922792231253	24.51339067495317	20.524120217019714
31	23.116333860600964	32.21094146114867	22.509267664730654	22.163457013519707
32	23.168554608632817	32.15750627711608	23.199978140151988	21.473960974099114
33	22.170743629524154	30.935024637870367	23.331744446232364	23.56248728637312
34	21.043595216336595	32.13215492560062	25.048805146779774	21.775444711283022
35	22.796026365405574	31.435827683675853	24.8142671941367	20.953878756781865
36	22.37826038115074	33.22696898026845	23.905110043082118	20.489660595498695
37	22.03336055694035	33.097631546189554	23.41144180878098	21.457566088089116
38	20.877217484235103	32.24874078167173	25.74406975720388	21.129971976889284
39	20.88526312274001	30.22382055493653	25.236890922394505	23.654025399928955
40	22.220990919054803	29.796794496176044	25.247517237400984	22.734697347368165
41	21.128150322888175	29.49136384198973	26.883666139399036	22.49681969572306
42	22.053246946452482	30.229285516939864	24.38845557137696	23.3290119652307
43	20.730726141645743	29.549808574525382	26.166086267461313	23.55337901636756
44	22.81651997291808	27.447771661743143	26.662335178264023	23.073373187074758
45	22.753217496379463	29.002249742691372	26.372843996587434	21.87168876434173
46	20.877521093235288	30.00613290180374	26.74446141281412	22.37188459214685
47	21.626828105692365	30.481584596093768	25.15871160484681	22.732875693367056
48	20.993499731306034	29.966056513779293	26.64624390125421	22.394199853660464
49	21.408077821058928	29.140695446775823	26.364039335582063	23.087187396583182
50	21.292250987488273	27.67396036688112	26.495502032662255	24.538286612968356
51	20.60154051206694	27.758060059932415	27.46188948025175	24.178509947748893
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	20608.0
1	15902.5
2	11197.0
3	6872.5
4	2548.0
5	2112.5
6	1677.0
7	1829.5
8	1982.0
9	2238.5
10	2495.0
11	2441.5
12	2388.0
13	2590.0
14	2792.0
15	2862.0
16	2932.0
17	2870.0
18	2808.0
19	2712.0
20	2616.0
21	2728.5
22	2841.0
23	3401.0
24	3961.0
25	4532.5
26	6518.5
27	7933.0
28	9227.0
29	10521.0
30	10927.5
31	11334.0
32	16172.5
33	21011.0
34	18434.5
35	15858.0
36	17996.5
37	20135.0
38	22198.5
39	24262.0
40	25639.5
41	27017.0
42	26658.5
43	26300.0
44	29729.5
45	33159.0
46	36583.0
47	40007.0
48	43703.0
49	47399.0
50	47090.5
51	46782.0
52	44443.5
53	42105.0
54	38628.5
55	35152.0
56	33275.5
57	31399.0
58	30320.0
59	29241.0
60	29015.5
61	28790.0
62	24895.5
63	21001.0
64	20615.0
65	20229.0
66	16999.0
67	13769.0
68	12152.5
69	10536.0
70	9680.0
71	8824.0
72	7843.0
73	6862.0
74	5738.0
75	4652.5
76	4691.0
77	3172.5
78	1654.0
79	1303.0
80	952.0
81	759.0
82	566.0
83	415.0
84	264.0
85	241.5
86	219.0
87	186.0
88	153.0
89	99.5
90	46.0
91	26.0
92	6.0
93	3.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	658742.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.529190177191946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.35689207892212	18.436742154186636
2	8.872039389765241	4.175038041226486
3	3.2198003493667735	2.272778842585197
4	1.6810631454265295	1.582162177944372
5	1.0710445733733187	1.2600405727575115
6	0.7517554763055141	1.0612918541242773
7	0.5759473064384946	0.9486101593662949
8	0.4281226165583034	0.8058702771325879
9	0.3481882560466551	0.7373328924587896
>10	2.9607758350798297	15.218334593161508
>50	0.8880815221744927	15.277623955290245
>100	0.8358414326348165	32.4704437450304
>500	0.006530011192459504	0.9504533840278521
>1k	0.003265005596229752	2.0232088847201353
>5k	0.0	0.0
>10k+	6.530011192459504E-4	2.780068465987707
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18094	2.746750624675518	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4597	0.6978452869256856	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3479	0.5281278558221579	No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT	1945	0.29525975268010846	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1789	0.2715782506656627	No Hit
GCCCCTGGGCTATATCTTTTATTTTGTTTTGGTTTAATATAACAGATAACC	1358	0.20615051112575183	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	895	0.13586502758287766	No Hit
CTACTTGGGGACTCCTAGGACTTTGATCTTTTAAAGGTTCCCTCCCCCATC	820	0.12447969007593261	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5180450009260075E-4	0.0	0.0	0.35871403371881555	0.0
2	1.5180450009260075E-4	0.0	0.0	0.48228289679419256	0.0
3	1.5180450009260075E-4	0.0	0.0	0.6046373238688287	0.0
4	1.5180450009260075E-4	0.0	0.0	1.1793691612194153	0.0
5	1.5180450009260075E-4	0.0	0.0	1.2789529132801614	0.0
6	1.5180450009260075E-4	0.0	0.0	1.8832866281488048	0.0
7	1.5180450009260075E-4	0.0	0.0	2.3648105024425345	0.0
8	1.5180450009260075E-4	0.0	0.0	2.790773929702372	0.0
9	1.5180450009260075E-4	0.0	0.0	3.378864563061107	0.0
10	1.5180450009260075E-4	0.0	0.0	4.3396352441471775	0.0
11	1.5180450009260075E-4	0.0	0.0	5.178810520659074	0.0
12	1.5180450009260075E-4	0.0	0.0	5.602496880417523	0.0
13	1.5180450009260075E-4	0.0	0.0	5.778286491524755	0.0
14	1.5180450009260075E-4	0.0	0.0	5.981552717148747	0.0
15	1.5180450009260075E-4	0.0	0.0	6.142465487246904	0.0
16	1.5180450009260075E-4	0.0	0.0	6.461862155441736	0.0
17	1.5180450009260075E-4	0.0	0.0	6.869305433690276	0.0
18	1.5180450009260075E-4	0.0	0.0	7.412006521521324	0.0
19	1.5180450009260075E-4	0.0	0.0	7.612540266143649	0.0
20	1.5180450009260075E-4	0.0	0.0	7.836300099280143	0.0
21	1.5180450009260075E-4	0.0	0.0	8.095582185438305	0.0
22	1.5180450009260075E-4	0.0	0.0	8.365642391103043	0.0
23	1.5180450009260075E-4	0.0	0.0	8.639194100269908	0.0
24	1.5180450009260075E-4	0.0	0.0	8.848532505897605	0.0
25	1.5180450009260075E-4	0.0	0.0	9.033126778010207	0.0
26	1.5180450009260075E-4	0.0	0.0	9.190092631105957	0.0
27	1.5180450009260075E-4	0.0	0.0	9.368614723214854	0.0
28	1.5180450009260075E-4	0.0	0.0	9.56277267883329	0.0
29	1.5180450009260075E-4	0.0	0.0	9.755564393950895	0.0
30	1.5180450009260075E-4	0.0	0.0	9.99055776009424	0.0
31	1.5180450009260075E-4	0.0	0.0	10.16862443870286	0.0
32	1.5180450009260075E-4	0.0	0.0	10.374471340828428	0.0
33	1.5180450009260075E-4	0.0	0.0	10.563771552443901	0.0
34	1.5180450009260075E-4	0.0	0.0	10.757170485561874	0.0
35	1.5180450009260075E-4	0.0	0.0	10.970304003691885	0.0
36	1.5180450009260075E-4	0.0	0.0	11.175088274316805	0.0
37	1.5180450009260075E-4	0.0	0.0	11.37152329743663	0.0
38	1.5180450009260075E-4	0.0	0.0	11.57706659056201	0.0
39	1.5180450009260075E-4	0.0	0.0	11.805532363201374	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGAAC	20	7.031092E-4	45.0	38
GTCATAT	25	3.8889855E-5	45.0	35
GACAGTA	20	7.031092E-4	45.0	13
TCGTTTA	25	3.8889855E-5	45.0	45
ACAGTCG	25	3.8889855E-5	45.0	17
CAAACGT	25	3.8889855E-5	45.0	39
GTACATA	20	7.031092E-4	45.0	10
GTCGAGT	20	7.031092E-4	45.0	24
CTCCGAT	25	3.8889855E-5	45.0	18
CGAGTAC	20	7.031092E-4	45.0	36
TTAGCGA	25	3.8889855E-5	45.0	12
TGGACGG	50	2.1827873E-11	45.0	2
GTTTAGC	35	1.2108649E-7	45.0	43
GTTTACG	20	7.031092E-4	45.0	1
CGCATTA	25	3.8889855E-5	45.0	22
TTTCGAA	20	7.031092E-4	45.0	29
TGGGTAT	70	0.0	45.0	6
TAGCGAG	20	7.031092E-4	45.0	1
ATTACAC	35	1.2108649E-7	45.0	19
TTGTCGA	20	7.031092E-4	45.0	22
>>END_MODULE