SRR3553254_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553254_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	813258
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14721	1.8101266756675005	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3466	0.4261870156826001	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2943	0.3618777804829464	No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT	2172	0.2670739175021949	TruSeq Adapter, Index 21 (95% over 23bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1758	0.2161675630611688	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGT	1392	0.17116339464229063	No Hit
AATCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT	1198	0.14730872613610932	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCC	1187	0.1459561418393671	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGT	25	3.889796E-5	45.0	11
AACCGGT	20	7.0320687E-4	45.0	41
AACCGCG	40	6.8102963E-9	45.0	1
ATCTCGC	25	3.889796E-5	45.0	38
ACCGGTT	25	3.889796E-5	45.0	36
ACACGAT	25	3.889796E-5	45.0	30
GTCGATA	25	3.889796E-5	45.0	9
ATTAGCG	40	6.8102963E-9	45.0	1
ATAGGTT	35	1.2113378E-7	45.0	43
CACGGTA	20	7.0320687E-4	45.0	4
CGACGTA	20	7.0320687E-4	45.0	25
CAACGAT	20	7.0320687E-4	45.0	32
TAGGTTG	20	7.0320687E-4	45.0	36
CGTTGTC	20	7.0320687E-4	45.0	45
CGCATCT	25	3.889796E-5	45.0	42
ATTACCG	50	2.1827873E-11	45.0	1
GCGCCTA	20	7.0320687E-4	45.0	9
CCGCTAA	25	3.889796E-5	45.0	25
ACGATAG	25	3.889796E-5	45.0	1
TCGGACG	45	3.8380676E-10	45.0	1