##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553254_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	813258
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.75460309028623	31.0	31.0	34.0	30.0	34.0
2	31.954453568240336	33.0	31.0	34.0	30.0	34.0
3	32.12138214441174	34.0	31.0	34.0	30.0	34.0
4	35.81763351851442	37.0	35.0	37.0	35.0	37.0
5	35.57328424681958	37.0	35.0	37.0	33.0	37.0
6	34.16771799355186	37.0	35.0	37.0	32.0	37.0
7	34.980444090313284	37.0	35.0	37.0	32.0	37.0
8	35.68711774123341	37.0	35.0	37.0	33.0	37.0
9	37.38532790332219	39.0	37.0	39.0	35.0	39.0
10	36.81719823229528	39.0	37.0	39.0	32.0	39.0
11	36.836513136052766	39.0	37.0	39.0	32.0	39.0
12	36.77784786623679	39.0	37.0	39.0	32.0	39.0
13	36.75630488725595	39.0	37.0	39.0	33.0	39.0
14	37.941281856434244	40.0	37.0	41.0	33.0	41.0
15	37.93208182397222	40.0	37.0	41.0	33.0	41.0
16	37.85242936435916	40.0	37.0	41.0	33.0	41.0
17	37.82262947305775	39.0	37.0	41.0	33.0	41.0
18	37.71451372135288	39.0	37.0	41.0	33.0	41.0
19	37.66476198205243	39.0	36.0	41.0	33.0	41.0
20	37.565660589874305	39.0	36.0	41.0	32.0	41.0
21	37.544982035221295	39.0	36.0	41.0	33.0	41.0
22	37.49497576414865	39.0	36.0	41.0	32.0	41.0
23	37.34575497566578	39.0	36.0	41.0	32.0	41.0
24	37.15269324125923	39.0	35.0	41.0	32.0	41.0
25	37.20804222030401	39.0	35.0	41.0	32.0	41.0
26	37.12029761773017	39.0	35.0	41.0	32.0	41.0
27	37.047093296346304	39.0	35.0	41.0	31.0	41.0
28	36.98616306264433	39.0	35.0	40.0	31.0	41.0
29	36.845486918050604	39.0	35.0	40.0	31.0	41.0
30	36.84738053606605	39.0	35.0	40.0	31.0	41.0
31	36.75966052593396	39.0	35.0	40.0	31.0	41.0
32	36.71665080454173	39.0	35.0	40.0	31.0	41.0
33	36.64817806895229	39.0	35.0	40.0	31.0	41.0
34	36.52882234174149	39.0	35.0	40.0	30.0	41.0
35	36.40514941137991	39.0	35.0	40.0	30.0	41.0
36	36.31772451054893	38.0	35.0	40.0	30.0	41.0
37	36.27880697146539	38.0	35.0	40.0	30.0	41.0
38	36.17231432091661	38.0	35.0	40.0	30.0	41.0
39	36.08475170241178	38.0	35.0	40.0	30.0	41.0
40	35.96226781660924	38.0	35.0	40.0	29.0	41.0
41	35.85965339412585	38.0	35.0	40.0	29.0	41.0
42	35.784466921936215	38.0	35.0	40.0	29.0	41.0
43	35.723626450646655	38.0	35.0	40.0	29.0	41.0
44	35.57272968725792	38.0	34.0	40.0	28.0	41.0
45	35.41536142282031	38.0	34.0	40.0	28.0	41.0
46	35.21630282149084	38.0	34.0	40.0	27.0	41.0
47	35.12754501031653	37.0	34.0	40.0	27.0	41.0
48	34.9804625346446	37.0	34.0	40.0	26.0	41.0
49	34.80973319659936	37.0	34.0	40.0	26.0	41.0
50	34.724830250670756	37.0	33.0	40.0	26.0	41.0
51	33.53130986722541	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	2.0
10	8.0
11	7.0
12	11.0
13	25.0
14	34.0
15	66.0
16	121.0
17	217.0
18	396.0
19	808.0
20	1283.0
21	2028.0
22	2757.0
23	3723.0
24	4745.0
25	5645.0
26	6680.0
27	8082.0
28	10008.0
29	12763.0
30	16222.0
31	21171.0
32	27591.0
33	37678.0
34	55571.0
35	75202.0
36	82950.0
37	112817.0
38	160334.0
39	164291.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.158158911440157	32.424519648131344	18.80522540202494	26.612096038403553
2	24.113995804529438	31.346141077001395	19.18038310105772	25.35948001741145
3	21.633479166512963	32.227288265224566	20.645231894429568	25.494000673832907
4	20.513416406601596	32.961864500564396	19.5602133640247	26.964505728809307
5	18.846909590806362	35.6267014895642	16.59584043440089	28.930548485228552
6	18.896217436533057	42.28067845628324	18.141106512324505	20.681997594859197
7	83.61602345135246	8.455865174397301	4.214160819813639	3.7139505544365994
8	85.44975887110854	7.464666809302829	3.6189007670382587	3.466673552550359
9	78.78803528523544	11.194479488673952	6.086506373131282	3.9309788529593312
10	34.507745389531976	36.34971927727732	12.877463240447682	16.265072092743015
11	24.55346273876187	31.64789033738371	24.63572445644556	19.162922467408865
12	25.859075471744514	26.91667342958815	28.14703329078841	19.077217807878927
13	24.13612900211249	28.00734822159757	27.377043939315694	20.479478836974245
14	20.938127875778658	29.97646503323669	27.726010687875192	21.359396403109468
15	19.817696229240905	31.84733504004879	27.417252581591573	20.91771614911873
16	21.205939566533623	29.52974332868536	27.297979239060666	21.96633786572035
17	21.719429750460492	29.273981934392285	27.504309825418254	21.502278489728965
18	22.97155392261742	28.36307789164078	25.96875776198943	22.696610423752364
19	21.400957629682093	31.294988798142782	25.918220294174787	21.38583327800034
20	23.211822078602363	30.619065536398043	25.904940375624957	20.26417200937464
21	24.800616778439313	28.803405561334777	25.517363493503908	20.878614166722002
22	21.572366948741976	28.543340489733886	25.73033403913641	24.153958522387732
23	20.922757599679315	30.507169926394823	25.854648832227905	22.71542364169796
24	19.779332020096945	30.839659738975822	27.606737345344285	21.77427089558295
25	23.420734871344642	30.392323223380522	24.40406365507625	21.782878250198586
26	24.018946017131096	29.82116376352892	23.486888539676215	22.673001679663773
27	20.531245926876835	29.752304926603856	26.86539818852074	22.85105095799857
28	20.663061414704806	30.48479080439418	28.20003000277895	20.652117778122072
29	21.752383622417486	30.995821744145154	25.403377525951175	21.848417107486185
30	21.832309058134072	29.74123832781233	26.90806607497252	21.518386539081078
31	22.046263301436937	31.884346664896015	23.94996421799724	22.119425815669814
32	22.521389276219846	29.29980399823918	26.076349694684836	22.102457030856137
33	21.41559013252867	29.711112586657617	25.815546849831172	23.057750430982544
34	21.01657776498971	30.521925391450193	26.33998067034078	22.121516173219323
35	21.978142237764644	28.86685406107287	27.652848173642315	21.502155527520173
36	23.66542966684619	31.43098500106977	24.94915512666337	19.954430205420667
37	22.56295050279247	31.10206109254382	24.32745819899712	22.00753020566659
38	22.80162015006308	29.576591930236162	26.03638697682654	21.585400942874216
39	22.308787617213728	28.93497512474516	25.695289809629905	23.060947448411206
40	23.16497347705156	28.91370266262367	26.230175417887065	21.6911484424377
41	20.29245331739743	28.90878417427188	27.68408057467618	23.11468193365451
42	21.347223144438786	29.120771022234027	26.28489360080073	23.247112232526455
43	21.02592289285811	28.36381566489355	27.072220623713505	23.53804081853483
44	22.296122509707867	27.053899254603092	27.250269902048302	23.39970833364074
45	22.88216039682364	28.592156486625402	26.0865555580148	22.43912755853616
46	21.776976064176438	28.336026205705938	28.122194924611872	21.764802805505756
47	21.56351366970875	28.772787971344886	27.834955204867335	21.828743154079024
48	20.88144229752428	29.101834842079636	28.594246844174908	21.422476016221175
49	21.01116742780274	28.922924828283275	27.258877256663936	22.80703048725005
50	21.560808501115268	27.007419539678672	28.19916926731738	23.232602691888673
51	21.170649412609528	26.863430793180026	28.410295379817967	23.555624414392483
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17280.0
1	13103.5
2	8927.0
3	5580.5
4	2234.0
5	1960.0
6	1686.0
7	1711.0
8	1736.0
9	1892.0
10	2048.0
11	2248.0
12	2448.0
13	2700.0
14	2952.0
15	2921.0
16	2890.0
17	3044.5
18	3199.0
19	3227.0
20	3255.0
21	3963.0
22	4671.0
23	5099.5
24	5528.0
25	5862.5
26	7245.5
27	8294.0
28	11103.5
29	13913.0
30	15289.5
31	16666.0
32	16785.0
33	16904.0
34	19789.5
35	22675.0
36	25812.5
37	28950.0
38	31546.0
39	34142.0
40	35261.0
41	36380.0
42	39642.5
43	42905.0
44	45574.5
45	48244.0
46	53380.5
47	58517.0
48	58981.5
49	59446.0
50	57424.0
51	55402.0
52	52771.5
53	50141.0
54	46839.0
55	43537.0
56	40569.5
57	37602.0
58	35378.5
59	33155.0
60	31889.0
61	30623.0
62	28221.5
63	25820.0
64	23488.5
65	21157.0
66	19230.0
67	17303.0
68	14771.0
69	12239.0
70	11069.5
71	9900.0
72	8769.5
73	7639.0
74	7022.0
75	5259.0
76	4113.0
77	3347.5
78	2582.0
79	2165.5
80	1749.0
81	1294.0
82	839.0
83	702.5
84	566.0
85	386.5
86	207.0
87	151.5
88	96.0
89	76.5
90	57.0
91	43.0
92	29.0
93	18.0
94	7.0
95	4.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	813258.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.507771303554993
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.25724115465488	16.61631074297914
2	9.242171772305934	3.97557033653855
3	3.3055372052150194	2.132842147354709
4	1.7141483247856069	1.4747004059944293
5	1.065625742454014	1.1459617381940965
6	0.7243951208350632	0.9348074755398974
7	0.5540466190133485	0.8341415581272873
8	0.43770504033893753	0.7531247924818544
9	0.33170312881760844	0.6420775531754487
>10	3.1458095777683055	15.768385270448599
>50	1.2588382050977067	19.620960205132825
>100	0.9557845631297402	32.1528341711004
>500	0.0023311818612920493	0.3336718927904709
>1k	0.004079568257261086	1.7693885945267798
>5k	0.0	0.0
>10k+	5.827954653230123E-4	1.845223115615523
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14721	1.8101266756675005	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3466	0.4261870156826001	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2943	0.3618777804829464	No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT	2172	0.2670739175021949	TruSeq Adapter, Index 21 (95% over 23bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1758	0.2161675630611688	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGT	1392	0.17116339464229063	No Hit
AATCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT	1198	0.14730872613610932	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCC	1187	0.1459561418393671	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2296220879474902E-4	0.0	0.0	0.31515214114094176	0.0
2	1.2296220879474902E-4	0.0	0.0	0.42975291973764784	0.0
3	1.2296220879474902E-4	0.0	0.0	0.566855782543793	0.0
4	1.2296220879474902E-4	0.0	0.0	1.210562945584304	0.0
5	1.2296220879474902E-4	0.0	0.0	1.3129904655103302	0.0
6	1.2296220879474902E-4	0.0	0.0	1.9113245735055788	0.0
7	1.2296220879474902E-4	0.0	0.0	2.343905624045506	0.0
8	1.2296220879474902E-4	0.0	0.0	2.6909049772642875	0.0
9	1.2296220879474902E-4	0.0	0.0	3.3424817216676628	0.0
10	1.2296220879474902E-4	0.0	0.0	4.242319165627636	0.0
11	1.2296220879474902E-4	0.0	0.0	5.098627987674268	0.0
12	1.2296220879474902E-4	0.0	0.0	5.453742846673503	0.0
13	1.2296220879474902E-4	0.0	0.0	5.63154620059071	0.0
14	1.2296220879474902E-4	0.0	0.0	5.812423609727786	0.0
15	1.2296220879474902E-4	0.0	0.0	5.9524775655450055	0.0
16	1.2296220879474902E-4	0.0	0.0	6.247217980026019	0.0
17	1.2296220879474902E-4	0.0	0.0	6.612292777937629	0.0
18	1.2296220879474902E-4	0.0	0.0	7.08483654633585	0.0
19	1.2296220879474902E-4	0.0	0.0	7.275796856594094	0.0
20	1.2296220879474902E-4	0.0	0.0	7.4844637249187835	0.0
21	1.2296220879474902E-4	0.0	0.0	7.724240032068544	0.0
22	1.2296220879474902E-4	0.0	0.0	7.9664755833942	0.0
23	1.2296220879474902E-4	0.0	0.0	8.23318061427001	0.0
24	1.2296220879474902E-4	0.0	0.0	8.430411997176787	0.0
25	1.2296220879474902E-4	0.0	0.0	8.604895371456536	0.0
26	1.2296220879474902E-4	0.0	0.0	8.75982775453792	0.0
27	1.2296220879474902E-4	0.0	0.0	8.933696317773695	0.0
28	1.2296220879474902E-4	0.0	0.0	9.119984064097741	0.0
29	1.2296220879474902E-4	0.0	0.0	9.316969522586929	0.0
30	1.2296220879474902E-4	0.0	0.0	9.542359251307703	0.0
31	1.2296220879474902E-4	0.0	0.0	9.7227448116096	0.0
32	1.2296220879474902E-4	0.0	0.0	9.913213273032666	0.0
33	1.2296220879474902E-4	0.0	0.0	10.116961653005566	0.0
34	1.2296220879474902E-4	0.0	0.0	10.309889358604526	0.0
35	2.4592441758949804E-4	0.0	0.0	10.527778392588822	0.0
36	2.4592441758949804E-4	0.0	0.0	10.715910572044788	0.0
37	3.6888662638424706E-4	0.0	0.0	10.914494539248308	0.0
38	3.6888662638424706E-4	0.0	0.0	11.112955544243032	0.0
39	3.6888662638424706E-4	0.0	0.0	11.320884639314952	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGT	25	3.889796E-5	45.0	11
AACCGGT	20	7.0320687E-4	45.0	41
AACCGCG	40	6.8102963E-9	45.0	1
ATCTCGC	25	3.889796E-5	45.0	38
ACCGGTT	25	3.889796E-5	45.0	36
ACACGAT	25	3.889796E-5	45.0	30
GTCGATA	25	3.889796E-5	45.0	9
ATTAGCG	40	6.8102963E-9	45.0	1
ATAGGTT	35	1.2113378E-7	45.0	43
CACGGTA	20	7.0320687E-4	45.0	4
CGACGTA	20	7.0320687E-4	45.0	25
CAACGAT	20	7.0320687E-4	45.0	32
TAGGTTG	20	7.0320687E-4	45.0	36
CGTTGTC	20	7.0320687E-4	45.0	45
CGCATCT	25	3.889796E-5	45.0	42
ATTACCG	50	2.1827873E-11	45.0	1
GCGCCTA	20	7.0320687E-4	45.0	9
CCGCTAA	25	3.889796E-5	45.0	25
ACGATAG	25	3.889796E-5	45.0	1
TCGGACG	45	3.8380676E-10	45.0	1
>>END_MODULE