Basic Statistics
Measure | Value |
---|---|
Filename | SRR3553253_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 806162 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 46 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 14960 | 1.8557064212900136 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 3365 | 0.41740990024337543 | No Hit |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2939 | 0.364566923273486 | No Hit |
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT | 2157 | 0.26756408761514433 | TruSeq Adapter, Index 21 (95% over 23bp) |
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1777 | 0.22042715980162797 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGT | 1266 | 0.15704039634713618 | No Hit |
AATGATACGGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCC | 1150 | 0.1426512289093259 | No Hit |
AATCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT | 1115 | 0.1383096697686073 | No Hit |
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA | 839 | 0.10407337483036908 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AGCGTAA | 35 | 1.2113196E-7 | 45.000004 | 25 |
AACCGCG | 70 | 0.0 | 45.000004 | 1 |
AGTTTCG | 35 | 1.2113196E-7 | 45.000004 | 14 |
TATCTCG | 35 | 1.2113196E-7 | 45.000004 | 1 |
CATCCGC | 35 | 1.2113196E-7 | 45.000004 | 38 |
TAATGCG | 35 | 1.2113196E-7 | 45.000004 | 1 |
ACACGTT | 40 | 6.8102963E-9 | 45.0 | 35 |
TCGCACT | 25 | 3.8897662E-5 | 45.0 | 12 |
CGAACGT | 20 | 7.0320326E-4 | 45.0 | 38 |
CGTATTG | 20 | 7.0320326E-4 | 45.0 | 13 |
ATCTACG | 20 | 7.0320326E-4 | 45.0 | 40 |
CGAAATT | 20 | 7.0320326E-4 | 45.0 | 13 |
ACGTTCA | 25 | 3.8897662E-5 | 45.0 | 40 |
ACGTTAG | 20 | 7.0320326E-4 | 45.0 | 1 |
GCGAAAT | 20 | 7.0320326E-4 | 45.0 | 12 |
CGTTGAC | 55 | 1.8189894E-12 | 45.0 | 18 |
ATTCGTT | 25 | 3.8897662E-5 | 45.0 | 10 |
TTATCGT | 25 | 3.8897662E-5 | 45.0 | 27 |
TTATCCG | 20 | 7.0320326E-4 | 45.0 | 1 |
CCGTCAA | 20 | 7.0320326E-4 | 45.0 | 13 |