FastQCFastQC Report
Sat 18 Jun 2016
SRR3553253_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553253_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences806162
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA149601.8557064212900136No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33650.41740990024337543No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29390.364566923273486No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT21570.26756408761514433TruSeq Adapter, Index 21 (95% over 23bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17770.22042715980162797No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGT12660.15704039634713618No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCC11500.1426512289093259No Hit
AATCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT11150.1383096697686073No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA8390.10407337483036908No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAA351.2113196E-745.00000425
AACCGCG700.045.0000041
AGTTTCG351.2113196E-745.00000414
TATCTCG351.2113196E-745.0000041
CATCCGC351.2113196E-745.00000438
TAATGCG351.2113196E-745.0000041
ACACGTT406.8102963E-945.035
TCGCACT253.8897662E-545.012
CGAACGT207.0320326E-445.038
CGTATTG207.0320326E-445.013
ATCTACG207.0320326E-445.040
CGAAATT207.0320326E-445.013
ACGTTCA253.8897662E-545.040
ACGTTAG207.0320326E-445.01
GCGAAAT207.0320326E-445.012
CGTTGAC551.8189894E-1245.018
ATTCGTT253.8897662E-545.010
TTATCGT253.8897662E-545.027
TTATCCG207.0320326E-445.01
CCGTCAA207.0320326E-445.013