##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3553253_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 806162 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.7322076704186 31.0 31.0 34.0 30.0 34.0 2 31.936129462812687 33.0 31.0 34.0 30.0 34.0 3 32.10347423966895 33.0 31.0 34.0 30.0 34.0 4 35.81150562790109 37.0 35.0 37.0 35.0 37.0 5 35.59250870172497 37.0 35.0 37.0 33.0 37.0 6 32.71046638268735 37.0 35.0 37.0 26.0 37.0 7 34.24484036707263 37.0 35.0 37.0 27.0 37.0 8 35.38603531300161 37.0 35.0 37.0 32.0 37.0 9 37.258109412252125 39.0 37.0 39.0 34.0 39.0 10 36.7748678305353 39.0 37.0 39.0 32.0 39.0 11 36.840352187277496 39.0 37.0 39.0 32.0 39.0 12 36.801430729803684 39.0 37.0 39.0 33.0 39.0 13 36.7938739360079 39.0 37.0 39.0 33.0 39.0 14 37.94895442851437 40.0 37.0 41.0 33.0 41.0 15 37.97176870157611 40.0 37.0 41.0 33.0 41.0 16 37.920766545681886 40.0 37.0 41.0 33.0 41.0 17 37.91049317630948 40.0 37.0 41.0 33.0 41.0 18 37.79738315623907 39.0 37.0 41.0 33.0 41.0 19 37.751510242358236 39.0 36.0 41.0 33.0 41.0 20 37.651583676729985 39.0 36.0 41.0 33.0 41.0 21 37.607598720852636 39.0 36.0 41.0 33.0 41.0 22 37.52605183573525 39.0 36.0 41.0 32.0 41.0 23 37.37529181479653 39.0 36.0 41.0 32.0 41.0 24 37.25012590521508 39.0 35.0 41.0 32.0 41.0 25 37.2759246900747 39.0 35.0 41.0 32.0 41.0 26 37.18676022933356 39.0 35.0 41.0 32.0 41.0 27 37.08468025037151 39.0 35.0 41.0 32.0 41.0 28 37.0489727871073 39.0 35.0 41.0 31.0 41.0 29 36.89053440871686 39.0 35.0 40.0 31.0 41.0 30 36.896613831959336 39.0 35.0 40.0 31.0 41.0 31 36.51088118765211 39.0 35.0 40.0 30.0 41.0 32 36.645734976344706 39.0 35.0 40.0 31.0 41.0 33 36.6270551080304 39.0 35.0 40.0 31.0 41.0 34 36.54758100729134 39.0 35.0 40.0 30.0 41.0 35 36.50327973781945 39.0 35.0 40.0 30.0 41.0 36 36.39798452420233 39.0 35.0 40.0 30.0 41.0 37 36.35110933038273 38.0 35.0 40.0 30.0 41.0 38 36.23773757631841 38.0 35.0 40.0 30.0 41.0 39 36.11992254658493 38.0 35.0 40.0 30.0 41.0 40 36.01560852533362 38.0 35.0 40.0 30.0 41.0 41 35.974766858274144 38.0 35.0 40.0 30.0 41.0 42 35.886529010298176 38.0 35.0 40.0 29.0 41.0 43 35.84484384031994 38.0 35.0 40.0 29.0 41.0 44 35.64904944663728 38.0 35.0 40.0 29.0 41.0 45 35.48756205328458 38.0 34.0 40.0 28.0 41.0 46 35.200370397016975 38.0 34.0 40.0 27.0 41.0 47 35.17093834737931 37.0 34.0 40.0 27.0 41.0 48 35.13164475626487 37.0 34.0 40.0 27.0 41.0 49 34.993387185205954 37.0 34.0 40.0 27.0 41.0 50 34.87814235848378 37.0 34.0 40.0 26.0 41.0 51 33.767318479412324 36.0 32.0 39.0 23.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 4.0 10 8.0 11 10.0 12 9.0 13 14.0 14 25.0 15 54.0 16 103.0 17 200.0 18 396.0 19 737.0 20 1264.0 21 1989.0 22 2797.0 23 3685.0 24 4548.0 25 5508.0 26 6444.0 27 7670.0 28 9699.0 29 12062.0 30 16039.0 31 20748.0 32 27721.0 33 37774.0 34 56190.0 35 76052.0 36 84867.0 37 115169.0 38 155914.0 39 158439.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.190453035494105 32.35962498852588 18.953262495627428 26.496659480352587 2 24.311490742555467 31.304626117331257 19.05559924680151 25.328283893311763 3 21.568121543808815 32.23917773350766 20.65019189691402 25.54250882576951 4 20.424802955237283 33.038644838134275 19.589238887469268 26.94731331915918 5 18.94569577826789 35.61529816587733 16.593439035826545 28.845567020028234 6 18.07403474735847 44.775491774606095 17.401589258734596 19.748884219300837 7 83.65440196883505 8.468149081698218 4.205730361887561 3.671718587579172 8 85.2653684991354 7.54786258841275 3.5816622465459793 3.60510666590586 9 78.81046241326185 11.257538807336491 6.055358600380568 3.8766401790210905 10 34.524574465181935 36.372342035471775 12.949258337654218 16.15382516169207 11 24.473368876230833 31.711864364730662 24.781743619768733 19.033023139269776 12 25.873955855026658 26.88057735293899 28.105393208809147 19.14007358322521 13 24.180871834693274 28.025012342432415 27.351946631074153 20.442169191800158 14 20.96886233784277 30.00935295883458 27.610331422220348 21.41145328110231 15 19.827900595661916 31.892473224984556 27.273922611088093 21.005703568265435 16 21.054453075188363 29.635110560904632 27.36794837762137 21.942487986285634 17 21.574819949340206 29.42125776208752 27.497450884561665 21.506471404010608 18 22.96659976530772 28.429397565253634 25.934365549356087 22.669637120082562 19 21.411205192008552 31.31504585926898 25.935357905731106 21.338391042991358 20 23.233419585641595 30.61220945666008 25.899633076230337 20.254737881467992 21 24.73659140470526 28.802771651355435 25.558386527769855 20.902250416169455 22 21.507339715838754 28.53322285099025 25.825702526291245 24.13373490687976 23 20.85449326562155 30.53554992668967 25.98782874906036 22.622128058628416 24 19.759552050332317 30.85967832768104 27.66478697829965 21.715982643687003 25 23.37358992361337 30.48146650425101 24.432434175761202 21.712509396374426 26 23.92075041989079 29.920537063270164 23.45074563177128 22.707966885067766 27 20.511137959864143 29.873896313644156 26.80156097657791 22.81340474991379 28 20.728464005993832 30.432965086421838 28.102044006043453 20.73652690154088 29 21.669341894060995 30.997987997449645 25.4041991560009 21.928470952488457 30 21.88207829195621 29.68646500331199 26.98489881686311 21.446557887868693 31 22.120119777414466 31.84806527720235 23.794224982075562 22.23758996330762 32 22.44412413385895 29.322890436413523 26.145613412688768 22.08737201703876 33 21.397932425492645 29.745138073985128 25.813918294337864 23.043011206184367 34 20.96104753138947 30.679193511974017 26.289753176160623 22.070005780475885 35 21.954148173692136 28.975565705156036 27.577955795485277 21.492330325666554 36 23.507806123335012 31.48635137850705 24.954785762663086 20.05105673549485 37 22.67149778828573 31.068693389169923 24.313723544399267 21.946085278145087 38 22.51520165921986 29.60906120606032 26.131844467985342 21.743892666734478 39 22.214889811229007 29.034238775829174 25.66432057080339 23.08655084213843 40 23.040406270699933 29.00942986645364 26.35946621150588 21.590697651340548 41 20.335738970579115 28.970231789640295 27.62298396600187 23.07104527377872 42 21.27488023498999 29.208893497832943 26.302033586301516 23.214192680875556 43 21.034605947687936 28.439941351738234 27.092693528099808 23.432759172474018 44 22.341663338137995 27.211900337649258 27.12234017480358 23.324096149409176 45 22.794301889694626 28.546371572959284 26.114602275969347 22.544724261376743 46 21.64292040557605 28.452841984613514 28.211203207295803 21.69303440251463 47 21.56725323198067 28.892331814201118 27.752858606582798 21.78755634723542 48 20.90572366348203 29.108789548502656 28.54389068202173 21.441596105993586 49 20.998384939999653 29.039572691344915 27.22715781691521 22.73488455174022 50 21.626546525388196 27.100260245459353 28.221126771046016 23.052066458106435 51 21.086208479189047 26.923000587971153 28.424931961566035 23.565858971273766 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 17576.0 1 13224.0 2 8872.0 3 5568.0 4 2264.0 5 2007.5 6 1751.0 7 1772.5 8 1794.0 9 1914.5 10 2035.0 11 2188.0 12 2341.0 13 2651.0 14 2961.0 15 2935.5 16 2910.0 17 3034.5 18 3159.0 19 3223.0 20 3287.0 21 3969.5 22 4652.0 23 5153.5 24 5655.0 25 5904.0 26 7140.0 27 8127.0 28 10927.0 29 13727.0 30 15050.0 31 16373.0 32 16845.5 33 17318.0 34 19833.5 35 22349.0 36 25728.5 37 29108.0 38 31418.5 39 33729.0 40 34951.5 41 36174.0 42 39383.0 43 42592.0 44 45081.5 45 47571.0 46 52786.5 47 58002.0 48 58315.5 49 58629.0 50 56744.5 51 54860.0 52 52417.0 53 49974.0 54 46200.5 55 42427.0 56 40059.5 57 37692.0 58 35299.0 59 32906.0 60 31608.5 61 30311.0 62 27901.5 63 25492.0 64 23138.5 65 20785.0 66 18867.0 67 16949.0 68 14572.0 69 12195.0 70 10951.5 71 9708.0 72 8612.0 73 7516.0 74 6924.5 75 5195.5 76 4058.0 77 3295.5 78 2533.0 79 2084.0 80 1635.0 81 1228.0 82 821.0 83 663.5 84 506.0 85 350.0 86 194.0 87 131.5 88 69.0 89 56.0 90 43.0 91 34.0 92 25.0 93 20.0 94 15.0 95 8.5 96 2.0 97 2.5 98 3.0 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 806162.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.797808474404924 #Duplication Level Percentage of deduplicated Percentage of total 1 76.37327666642214 16.647700573374085 2 9.12748438151568 3.9791831280280214 3 3.378098819835567 2.20905453247167 4 1.8486970316792268 1.6119017529497872 5 1.1805758754785562 1.2866983411592243 6 0.8681114101923614 1.1353755752291197 7 0.642437282875377 0.9802607394254197 8 0.5136632753785565 0.895738695762983 9 0.38983007424208194 0.764769716630276 >10 3.5396872158460115 17.232522617611092 >50 1.2773441602556217 20.186143416479364 >100 0.8544474712459692 29.176864286362342 >500 0.0017308186453328209 0.28082247698939294 >1k 0.004038576839109915 1.7315918878938428 >5k 0.0 0.0 >10k+ 5.769395484442736E-4 1.8813722596333713 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 14960 1.8557064212900136 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3365 0.41740990024337543 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2939 0.364566923273486 No Hit CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT 2157 0.26756408761514433 TruSeq Adapter, Index 21 (95% over 23bp) TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1777 0.22042715980162797 No Hit AATGATACCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGT 1266 0.15704039634713618 No Hit AATGATACGGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCC 1150 0.1426512289093259 No Hit AATCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT 1115 0.1383096697686073 No Hit AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA 839 0.10407337483036908 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.240445468776747E-4 0.0 0.0 0.3075064317097556 0.0 2 1.240445468776747E-4 0.0 0.0 0.4045092673680972 0.0 3 1.240445468776747E-4 0.0 0.0 0.5310347051833254 0.0 4 1.240445468776747E-4 0.0 0.0 1.1387289403370537 0.0 5 1.240445468776747E-4 0.0 0.0 1.2352355978078848 0.0 6 1.240445468776747E-4 0.0 0.0 1.7944284151324423 0.0 7 1.240445468776747E-4 0.0 0.0 2.2084891126101205 0.0 8 1.240445468776747E-4 0.0 0.0 2.573552214071117 0.0 9 1.240445468776747E-4 0.0 0.0 3.205063002225359 0.0 10 1.240445468776747E-4 0.0 0.0 4.0615905984157035 0.0 11 1.240445468776747E-4 0.0 0.0 4.89765084437123 0.0 12 1.240445468776747E-4 0.0 0.0 5.2515499366132365 0.0 13 1.240445468776747E-4 0.0 0.0 5.41888603035122 0.0 14 1.240445468776747E-4 0.0 0.0 5.5950292869175176 0.0 15 1.240445468776747E-4 0.0 0.0 5.741773985873807 0.0 16 1.240445468776747E-4 0.0 0.0 6.03824045291145 0.0 17 1.240445468776747E-4 0.0 0.0 6.388418208747125 0.0 18 1.240445468776747E-4 0.0 0.0 6.85011201222583 0.0 19 1.240445468776747E-4 0.0 0.0 7.047466886308211 0.0 20 1.240445468776747E-4 0.0 0.0 7.250279720453209 0.0 21 1.240445468776747E-4 0.0 0.0 7.473311815739268 0.0 22 1.240445468776747E-4 0.0 0.0 7.729587849588544 0.0 23 1.240445468776747E-4 0.0 0.0 7.9929344226098475 0.0 24 1.240445468776747E-4 0.0 0.0 8.177760797457584 0.0 25 1.240445468776747E-4 0.0 0.0 8.34001106477358 0.0 26 1.240445468776747E-4 0.0 0.0 8.49531483746443 0.0 27 1.240445468776747E-4 0.0 0.0 8.673938984968283 0.0 28 1.240445468776747E-4 0.0 0.0 8.850702464268968 0.0 29 1.240445468776747E-4 0.0 0.0 9.044832180132529 0.0 30 1.240445468776747E-4 0.0 0.0 9.279772551918844 0.0 31 1.240445468776747E-4 0.0 0.0 9.449713581141259 0.0 32 1.240445468776747E-4 0.0 0.0 9.640370049692246 0.0 33 1.240445468776747E-4 0.0 0.0 9.836112344665215 0.0 34 1.240445468776747E-4 0.0 0.0 10.024536011372405 0.0 35 1.240445468776747E-4 0.0 0.0 10.258608071330576 0.0 36 1.240445468776747E-4 0.0 0.0 10.449760718069072 0.0 37 1.240445468776747E-4 0.0 0.0 10.637936295682506 0.0 38 1.240445468776747E-4 0.0 0.0 10.839384639811849 0.0 39 1.240445468776747E-4 0.0 0.0 11.037855914816129 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCGTAA 35 1.2113196E-7 45.000004 25 AACCGCG 70 0.0 45.000004 1 AGTTTCG 35 1.2113196E-7 45.000004 14 TATCTCG 35 1.2113196E-7 45.000004 1 CATCCGC 35 1.2113196E-7 45.000004 38 TAATGCG 35 1.2113196E-7 45.000004 1 ACACGTT 40 6.8102963E-9 45.0 35 TCGCACT 25 3.8897662E-5 45.0 12 CGAACGT 20 7.0320326E-4 45.0 38 CGTATTG 20 7.0320326E-4 45.0 13 ATCTACG 20 7.0320326E-4 45.0 40 CGAAATT 20 7.0320326E-4 45.0 13 ACGTTCA 25 3.8897662E-5 45.0 40 ACGTTAG 20 7.0320326E-4 45.0 1 GCGAAAT 20 7.0320326E-4 45.0 12 CGTTGAC 55 1.8189894E-12 45.0 18 ATTCGTT 25 3.8897662E-5 45.0 10 TTATCGT 25 3.8897662E-5 45.0 27 TTATCCG 20 7.0320326E-4 45.0 1 CCGTCAA 20 7.0320326E-4 45.0 13 >>END_MODULE