Basic Statistics
Measure | Value |
---|---|
Filename | SRR3553251_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 538066 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 45 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 15747 | 2.926592648485502 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 3370 | 0.626317217590407 | No Hit |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 3311 | 0.6153520200124148 | No Hit |
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT | 2115 | 0.39307445555006265 | Illumina Single End Adapter 1 (95% over 21bp) |
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1570 | 0.2917857660584389 | No Hit |
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG | 654 | 0.12154642738994846 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCACGAA | 25 | 3.8880295E-5 | 45.000004 | 11 |
CCTTACG | 25 | 3.8880295E-5 | 45.000004 | 1 |
GGTCGAG | 30 | 2.1633405E-6 | 45.000004 | 9 |
GAATGCG | 25 | 3.8880295E-5 | 45.000004 | 1 |
CATGCGT | 25 | 3.8880295E-5 | 45.000004 | 45 |
CGCGGAC | 30 | 2.1633405E-6 | 45.000004 | 35 |
TAGAATC | 30 | 2.1633405E-6 | 45.000004 | 19 |
TAGGTCG | 25 | 3.8880295E-5 | 45.000004 | 40 |
CATTGCG | 25 | 3.8880295E-5 | 45.000004 | 1 |
ATTACGG | 50 | 2.1827873E-11 | 45.000004 | 2 |
TAACGGC | 25 | 3.8880295E-5 | 45.000004 | 25 |
TATGCGT | 25 | 3.8880295E-5 | 45.000004 | 45 |
TCGGCCC | 30 | 2.1633405E-6 | 45.000004 | 42 |
ATCGTAC | 25 | 3.8880295E-5 | 45.000004 | 35 |
CTGAACG | 30 | 2.1633405E-6 | 45.000004 | 1 |
ATCCGAG | 25 | 3.8880295E-5 | 45.000004 | 1 |
CCGTACA | 25 | 3.8880295E-5 | 45.000004 | 18 |
AGGTTAC | 25 | 3.8880295E-5 | 45.000004 | 32 |
GCTTACG | 30 | 2.1633405E-6 | 45.000004 | 1 |
TCATGCG | 25 | 3.8880295E-5 | 45.000004 | 1 |