FastQCFastQC Report
Sat 18 Jun 2016
SRR3553251_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553251_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538066
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA157472.926592648485502No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33700.626317217590407No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33110.6153520200124148No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT21150.39307445555006265Illumina Single End Adapter 1 (95% over 21bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15700.2917857660584389No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG6540.12154642738994846No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAA253.8880295E-545.00000411
CCTTACG253.8880295E-545.0000041
GGTCGAG302.1633405E-645.0000049
GAATGCG253.8880295E-545.0000041
CATGCGT253.8880295E-545.00000445
CGCGGAC302.1633405E-645.00000435
TAGAATC302.1633405E-645.00000419
TAGGTCG253.8880295E-545.00000440
CATTGCG253.8880295E-545.0000041
ATTACGG502.1827873E-1145.0000042
TAACGGC253.8880295E-545.00000425
TATGCGT253.8880295E-545.00000445
TCGGCCC302.1633405E-645.00000442
ATCGTAC253.8880295E-545.00000435
CTGAACG302.1633405E-645.0000041
ATCCGAG253.8880295E-545.0000041
CCGTACA253.8880295E-545.00000418
AGGTTAC253.8880295E-545.00000432
GCTTACG302.1633405E-645.0000041
TCATGCG253.8880295E-545.0000041