##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553251_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	538066
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.621819628075365	31.0	31.0	34.0	30.0	34.0
2	31.821224533793252	33.0	31.0	34.0	30.0	34.0
3	32.01406333052079	33.0	31.0	34.0	30.0	34.0
4	35.74232157393331	37.0	35.0	37.0	35.0	37.0
5	35.50110581229812	37.0	35.0	37.0	33.0	37.0
6	32.93270528150822	37.0	35.0	37.0	28.0	37.0
7	34.31587760609293	37.0	35.0	37.0	29.0	37.0
8	35.32852847048503	37.0	35.0	37.0	32.0	37.0
9	37.113248188883894	39.0	37.0	39.0	34.0	39.0
10	36.65179736314876	38.0	37.0	39.0	32.0	39.0
11	36.713230347206476	39.0	37.0	39.0	32.0	39.0
12	36.661593559154454	39.0	35.0	39.0	32.0	39.0
13	36.625841439526006	39.0	35.0	39.0	32.0	39.0
14	37.77197592860355	40.0	37.0	41.0	33.0	41.0
15	37.82044210189828	40.0	37.0	41.0	33.0	41.0
16	37.7542383276401	40.0	37.0	41.0	33.0	41.0
17	37.69887708942769	39.0	36.0	41.0	33.0	41.0
18	37.55969156200169	39.0	36.0	41.0	33.0	41.0
19	37.463417127266915	39.0	35.0	41.0	32.0	41.0
20	37.33517263681407	39.0	35.0	41.0	32.0	41.0
21	37.263556887073335	39.0	35.0	41.0	32.0	41.0
22	37.20458828470857	39.0	35.0	41.0	32.0	41.0
23	37.038510145595524	39.0	35.0	40.0	31.0	41.0
24	36.9043370144183	39.0	35.0	40.0	31.0	41.0
25	36.9236394791717	39.0	35.0	40.0	31.0	41.0
26	36.83435303475782	39.0	35.0	40.0	31.0	41.0
27	36.76276516263804	39.0	35.0	40.0	31.0	41.0
28	36.68815535640609	39.0	35.0	40.0	31.0	41.0
29	36.43887552828092	38.0	35.0	40.0	30.0	41.0
30	36.428068303888374	38.0	35.0	40.0	30.0	41.0
31	36.06538603070999	38.0	35.0	40.0	30.0	41.0
32	36.1446216635134	38.0	35.0	40.0	30.0	41.0
33	36.11528511372211	38.0	35.0	40.0	30.0	41.0
34	36.07798485687629	38.0	35.0	40.0	30.0	41.0
35	35.97280631000658	38.0	35.0	40.0	30.0	41.0
36	35.881237617690026	38.0	35.0	40.0	29.0	41.0
37	35.80020852460479	38.0	35.0	40.0	29.0	41.0
38	35.6748521556835	38.0	35.0	40.0	29.0	41.0
39	35.535804157854244	38.0	34.0	40.0	28.0	41.0
40	35.409239758691314	38.0	34.0	40.0	27.0	41.0
41	35.374972587006056	38.0	34.0	40.0	27.0	41.0
42	35.28350239561689	38.0	34.0	40.0	27.0	41.0
43	35.22032613099508	38.0	34.0	40.0	27.0	41.0
44	35.02666959071935	38.0	34.0	40.0	26.0	41.0
45	34.82052573476116	37.0	34.0	40.0	25.0	41.0
46	34.5437195436991	37.0	33.0	40.0	24.0	41.0
47	34.49933465411306	37.0	33.0	40.0	24.0	41.0
48	34.48157103403672	37.0	33.0	40.0	24.0	41.0
49	34.33589745495906	37.0	33.0	40.0	24.0	41.0
50	34.23690402292655	36.0	33.0	40.0	24.0	41.0
51	33.14447112436021	35.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	8.0
11	14.0
12	10.0
13	21.0
14	33.0
15	66.0
16	120.0
17	221.0
18	387.0
19	739.0
20	1177.0
21	1794.0
22	2361.0
23	3194.0
24	3779.0
25	4452.0
26	5334.0
27	6295.0
28	7600.0
29	9643.0
30	12201.0
31	15715.0
32	20609.0
33	27690.0
34	40404.0
35	54594.0
36	55457.0
37	73349.0
38	96333.0
39	94455.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.37717677757004	33.46020748384027	20.24063962413533	26.92197611445436
2	22.637929175974694	33.964049020008694	19.647589700891714	23.750432103124897
3	20.49506937810603	34.92396843509904	18.553300152769364	26.027662034025568
4	19.77489750328027	36.63937137823241	17.57739756832805	26.008333550159275
5	17.801533640854466	37.671772607821346	16.328480149275368	28.19821360204882
6	17.735184902967294	44.68280842870577	16.35951723394528	21.222489434381657
7	81.73848561328909	10.710210271602369	3.984455438552148	3.5668486765564076
8	82.80657019770808	9.94915121936714	3.7692402047332485	3.475038378191523
9	75.76914356231391	13.74887095635108	6.583950667761948	3.8980348135730565
10	31.36957176257188	41.99819353016173	11.511784799634246	15.12044990763215
11	23.32650641371133	31.369757613378287	27.435110190943117	17.868625781967268
12	24.97128605041017	28.122200622228497	27.996937178710418	18.90957614865091
13	22.37699464377976	29.8524716298744	26.51979496939037	21.250738756955467
14	18.85735207205064	31.410830641594156	27.680433255399894	22.051384030955308
15	18.51352808019834	33.18068787100467	26.838343251571366	21.46744079722562
16	21.223232837607284	31.79963052859686	25.548724505915633	21.42841212788022
17	19.4879438581884	32.00350886322496	27.364858586121404	21.143688692465236
18	21.340504696449877	30.423033605542816	25.800738199403046	22.43572349860426
19	20.41794129344727	33.39627480643638	24.55813970776819	21.62764419234815
20	21.679310716529198	31.782346403601046	25.6708284857248	20.867514394144955
21	22.468804942144644	31.808179665691572	25.69833440507298	20.024680987090804
22	21.809963833433073	30.480089803109657	24.108380756264104	23.60156560719317
23	21.22992346663792	32.40048618570956	23.501949574959205	22.867640772693314
24	18.254823757680285	33.37787557660213	26.179502142859796	22.18779852285779
25	19.232027297766447	33.69233514104218	24.554608542446466	22.521029018744912
26	20.707310999022425	32.5385733348697	23.730917768452198	23.023197897655677
27	19.71802715651983	31.260477339211175	24.134399869161033	24.88709563510796
28	19.35710489047812	33.074195358933665	25.29094943742961	22.27775031315861
29	20.620332821624114	33.517077830600705	24.16171993770281	21.70086941007237
30	20.370177636200765	31.68849174636569	25.88437106228604	22.05695955514751
31	23.68315411120569	32.112045734166436	22.55113684938279	21.653663305245082
32	22.47363706311122	30.21134953704564	24.662216159355914	22.652797240487228
33	20.524991357937502	30.38995216200243	25.142082941497883	23.942973538562185
34	20.399913765225826	30.9424866094494	26.07300963078879	22.584589994535985
35	20.54524909583583	29.777202053279712	27.357052852252327	22.32049599863214
36	21.80903457940104	32.13211762125836	24.979463485892065	21.079384313448536
37	21.928164946307703	31.772868012474305	24.11358457884349	22.185382462374502
38	22.387773990551345	30.466894395854784	27.238851739377694	19.906479874216174
39	21.98540699468095	29.385986105793716	26.52221102987366	22.106395869651678
40	23.344533941932774	29.294361658235236	26.49805042504079	20.863053974791196
41	20.001821337902786	28.6840276099958	28.605784420498598	22.708366631602814
42	21.499407135927562	29.74951028312512	26.106276925135578	22.64480565581174
43	20.942226418320427	30.1095032951348	25.763010485702498	23.185259800842275
44	21.937829188240848	28.485167247140687	25.98250028806875	23.594503276549716
45	22.63662822032985	29.13155635182301	26.710292045957186	21.521523381889953
46	20.29881092654061	28.73606583578966	27.446818791746736	23.51830444592299
47	19.8345556121368	30.108202339489953	27.52208836834143	22.535153680031815
48	20.560303011154765	28.762642501105812	28.98120304944003	21.69585143829939
49	20.13693487416042	29.67108124282151	27.893046577929105	22.298937305088966
50	20.28059754751276	27.075303029739846	28.574561485022283	24.069537937725112
51	20.134332962870726	27.611668457029438	28.49297298100976	23.761025599090075
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	17820.0
1	13635.0
2	9450.0
3	5744.0
4	2038.0
5	1657.5
6	1277.0
7	1410.5
8	1544.0
9	1685.0
10	1826.0
11	1914.5
12	2003.0
13	2345.5
14	2688.0
15	2653.0
16	2618.0
17	2644.0
18	2670.0
19	2722.5
20	2775.0
21	2791.5
22	2808.0
23	3079.5
24	3351.0
25	3567.5
26	4726.5
27	5669.0
28	7460.5
29	9252.0
30	9314.0
31	9376.0
32	11249.0
33	13122.0
34	14739.5
35	16357.0
36	17737.5
37	19118.0
38	19622.5
39	20127.0
40	20426.0
41	20725.0
42	22018.5
43	23312.0
44	24822.0
45	26332.0
46	28847.0
47	31362.0
48	33327.0
49	35292.0
50	39890.0
51	44488.0
52	41502.5
53	38517.0
54	35434.5
55	32352.0
56	29564.0
57	26776.0
58	25079.0
59	23382.0
60	21693.0
61	20004.0
62	17548.5
63	15093.0
64	14432.0
65	13771.0
66	12057.0
67	10343.0
68	9251.5
69	8160.0
70	7049.0
71	5938.0
72	5278.0
73	4618.0
74	3830.0
75	2475.0
76	1908.0
77	1650.5
78	1393.0
79	1046.5
80	700.0
81	510.5
82	321.0
83	272.0
84	223.0
85	209.0
86	195.0
87	131.5
88	68.0
89	51.0
90	34.0
91	25.5
92	17.0
93	16.5
94	16.0
95	12.0
96	8.0
97	4.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	538066.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.564744172480474
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.89549073930935	19.913782929402657
2	8.703643983523818	4.450128636142706
3	3.1425095236319422	2.410123560937023
4	1.7747576434233299	1.8148490048908703
5	1.0781812354685165	1.3781713728160778
6	0.797017264516796	1.2225325481053255
7	0.5673525375878141	1.015295573532806
8	0.42558074749908154	0.8703890347637624
9	0.37638484816204426	0.8659964118294512
>10	3.5802732492397444	20.81977508706865
>50	1.1430443249267361	20.700659277863185
>100	0.5113619348356451	19.5179145088954
>500	7.336613125332067E-4	0.12266334901759118
>1k	0.002934645250132827	1.9442328377925846
>5k	0.0	0.0
>10k+	7.336613125332067E-4	2.953485866941909
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15747	2.926592648485502	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3370	0.626317217590407	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3311	0.6153520200124148	No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT	2115	0.39307445555006265	Illumina Single End Adapter 1 (95% over 21bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1570	0.2917857660584389	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	654	0.12154642738994846	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.85850806406649E-4	0.0	0.0	0.4649987176294358	0.0
2	1.85850806406649E-4	0.0	0.0	0.6220426490430542	0.0
3	1.85850806406649E-4	0.0	0.0	0.785219657068092	0.0
4	1.85850806406649E-4	0.0	0.0	1.554084443172399	0.0
5	1.85850806406649E-4	0.0	0.0	1.6851092616890864	0.0
6	1.85850806406649E-4	0.0	0.0	2.405466987321258	0.0
7	1.85850806406649E-4	0.0	0.0	2.981976188794683	0.0
8	1.85850806406649E-4	0.0	0.0	3.40032635401605	0.0
9	1.85850806406649E-4	0.0	0.0	4.0405823820869555	0.0
10	1.85850806406649E-4	0.0	0.0	5.025405805235789	0.0
11	1.85850806406649E-4	0.0	0.0	5.880505365512781	0.0
12	1.85850806406649E-4	0.0	0.0	6.358699490397089	0.0
13	1.85850806406649E-4	0.0	0.0	6.561276869380336	0.0
14	1.85850806406649E-4	0.0	0.0	6.753260752398405	0.0
15	1.85850806406649E-4	0.0	0.0	6.916066058810629	0.0
16	1.85850806406649E-4	0.0	0.0	7.247995599052905	0.0
17	1.85850806406649E-4	0.0	0.0	7.676567558626637	0.0
18	1.85850806406649E-4	0.0	0.0	8.239137949619563	0.0
19	1.85850806406649E-4	0.0	0.0	8.459928707630663	0.0
20	1.85850806406649E-4	0.0	0.0	8.713429207569332	0.0
21	1.85850806406649E-4	0.0	0.0	8.979381711537247	0.0
22	1.85850806406649E-4	0.0	0.0	9.263547594533012	0.0
23	1.85850806406649E-4	0.0	0.0	9.553103150914572	0.0
24	1.85850806406649E-4	0.0	0.0	9.782443046020378	0.0
25	1.85850806406649E-4	0.0	0.0	9.971825017748753	0.0
26	1.85850806406649E-4	0.0	0.0	10.14057754996599	0.0
27	1.85850806406649E-4	0.0	0.0	10.336464299918598	0.0
28	1.85850806406649E-4	0.0	0.0	10.539413380514658	0.0
29	1.85850806406649E-4	0.0	0.0	10.744778521594005	0.0
30	1.85850806406649E-4	0.0	0.0	11.042139811844644	0.0
31	3.71701612813298E-4	0.0	0.0	11.236353904539591	0.0
32	3.71701612813298E-4	0.0	0.0	11.445250210940666	0.0
33	3.71701612813298E-4	0.0	0.0	11.658978638308312	0.0
34	3.71701612813298E-4	0.0	0.0	11.8570955979378	0.0
35	3.71701612813298E-4	0.0	0.0	12.083461880141098	0.0
36	3.71701612813298E-4	0.0	0.0	12.278976928480892	0.0
37	3.71701612813298E-4	0.0	0.0	12.494563863912605	0.0
38	3.71701612813298E-4	0.0	0.0	12.714053666278858	0.0
39	3.71701612813298E-4	0.0	0.0	12.922020718647898	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAA	25	3.8880295E-5	45.000004	11
CCTTACG	25	3.8880295E-5	45.000004	1
GGTCGAG	30	2.1633405E-6	45.000004	9
GAATGCG	25	3.8880295E-5	45.000004	1
CATGCGT	25	3.8880295E-5	45.000004	45
CGCGGAC	30	2.1633405E-6	45.000004	35
TAGAATC	30	2.1633405E-6	45.000004	19
TAGGTCG	25	3.8880295E-5	45.000004	40
CATTGCG	25	3.8880295E-5	45.000004	1
ATTACGG	50	2.1827873E-11	45.000004	2
TAACGGC	25	3.8880295E-5	45.000004	25
TATGCGT	25	3.8880295E-5	45.000004	45
TCGGCCC	30	2.1633405E-6	45.000004	42
ATCGTAC	25	3.8880295E-5	45.000004	35
CTGAACG	30	2.1633405E-6	45.000004	1
ATCCGAG	25	3.8880295E-5	45.000004	1
CCGTACA	25	3.8880295E-5	45.000004	18
AGGTTAC	25	3.8880295E-5	45.000004	32
GCTTACG	30	2.1633405E-6	45.000004	1
TCATGCG	25	3.8880295E-5	45.000004	1
>>END_MODULE