SRR3553250_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553250_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	973083
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16024	1.6467248939710177	No Hit
AATCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCT	5734	0.589261142163618	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGT	5502	0.5654193938235484	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCC	5054	0.5193801556496208	No Hit
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGCT	3931	0.40397376174488714	Illumina Single End Adapter 1 (95% over 22bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3584	0.3683139053914209	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2987	0.3069625098783968	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1576	0.16195946286185248	No Hit
AATGACTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTT	1526	0.15682115502994093	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCT	1327	0.1363706898589329	No Hit
AACTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG	1315	0.13513749597927413	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1083	0.11129574763920447	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1016	0.10441041514444298	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGTT	25	3.8903625E-5	45.000004	35
ACACGTA	25	3.8903625E-5	45.000004	32
CGACGTG	35	1.2116652E-7	45.000004	1
CGCGGAA	25	3.8903625E-5	45.000004	4
GGCGTAC	35	1.2116652E-7	45.000004	8
TAGCGAT	25	3.8903625E-5	45.000004	45
ATATGCG	25	3.8903625E-5	45.000004	1
TAGCCGA	35	1.2116652E-7	45.000004	41
GTTACGT	25	3.8903625E-5	45.000004	45
CTAAACG	35	1.2116652E-7	45.000004	1
ACTACGC	25	3.8903625E-5	45.000004	13
ACTCGTC	25	3.8903625E-5	45.000004	18
CATACGT	25	3.8903625E-5	45.000004	23
CGTAATC	25	3.8903625E-5	45.000004	23
GTATACG	70	0.0	45.000004	1
TCGAGCA	35	1.2116652E-7	45.000004	31
TATCGTG	50	2.1827873E-11	45.000004	1
ATACCCG	35	1.2116652E-7	45.000004	1
TCTATCG	25	3.8903625E-5	45.000004	26
CTAGCGA	25	3.8903625E-5	45.000004	44