##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553250_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	973083
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53482796431548	31.0	31.0	34.0	30.0	34.0
2	31.73604307135157	31.0	31.0	34.0	30.0	34.0
3	31.900734058656866	33.0	31.0	34.0	30.0	34.0
4	35.63009938515008	37.0	35.0	37.0	33.0	37.0
5	35.36927271363285	37.0	35.0	37.0	33.0	37.0
6	33.995848247271816	37.0	35.0	37.0	31.0	37.0
7	34.85575228423475	37.0	35.0	37.0	32.0	37.0
8	35.57881393467977	37.0	35.0	37.0	32.0	37.0
9	37.29038530115108	39.0	37.0	39.0	34.0	39.0
10	36.58695198662396	38.0	35.0	39.0	32.0	39.0
11	36.61579125316134	39.0	35.0	39.0	32.0	39.0
12	36.65581250520253	39.0	35.0	39.0	32.0	39.0
13	36.67131991823925	39.0	35.0	39.0	32.0	39.0
14	37.81850469076122	39.0	37.0	41.0	33.0	41.0
15	37.83336159402641	39.0	37.0	41.0	33.0	41.0
16	37.704834017242106	39.0	37.0	41.0	32.0	41.0
17	37.66430098974085	39.0	37.0	41.0	32.0	41.0
18	37.54930771578581	39.0	36.0	41.0	32.0	41.0
19	37.53504377324442	39.0	36.0	41.0	32.0	41.0
20	37.50496000855014	39.0	36.0	41.0	32.0	41.0
21	37.52224630375826	39.0	36.0	41.0	32.0	41.0
22	37.477172039795164	39.0	36.0	41.0	32.0	41.0
23	37.31795232266929	39.0	36.0	40.0	32.0	41.0
24	37.123431403076616	39.0	36.0	40.0	31.0	41.0
25	37.223450620347904	39.0	36.0	40.0	32.0	41.0
26	37.12100920476465	39.0	36.0	40.0	31.0	41.0
27	37.10611530568307	39.0	35.0	40.0	31.0	41.0
28	37.05438898840078	39.0	35.0	40.0	31.0	41.0
29	36.85293135323503	39.0	35.0	40.0	31.0	41.0
30	36.8706040491921	39.0	35.0	40.0	31.0	41.0
31	36.78511802179259	39.0	35.0	40.0	31.0	41.0
32	36.67996357967409	39.0	35.0	40.0	31.0	41.0
33	36.592778827705345	39.0	35.0	40.0	30.0	41.0
34	36.554258989212634	39.0	35.0	40.0	30.0	41.0
35	36.43988950582838	39.0	35.0	40.0	30.0	41.0
36	36.347414352115905	38.0	35.0	40.0	30.0	41.0
37	36.329136363496225	38.0	35.0	40.0	30.0	41.0
38	36.19596581175501	38.0	35.0	40.0	30.0	41.0
39	36.116834843482	38.0	35.0	40.0	30.0	41.0
40	36.00801370489465	38.0	35.0	40.0	30.0	41.0
41	35.89856672041336	38.0	35.0	40.0	29.0	41.0
42	35.86427776458945	38.0	35.0	40.0	29.0	41.0
43	35.76906697578727	38.0	35.0	40.0	29.0	41.0
44	35.677715056166846	38.0	35.0	40.0	28.0	41.0
45	35.54521865041317	38.0	34.0	40.0	28.0	41.0
46	35.36671897464039	38.0	34.0	40.0	27.0	41.0
47	35.30637777044713	38.0	34.0	40.0	27.0	41.0
48	35.202941578467616	38.0	34.0	40.0	27.0	41.0
49	35.00054054998392	37.0	34.0	40.0	26.0	41.0
50	34.88494095570471	37.0	33.0	40.0	26.0	41.0
51	33.76255057379483	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	7.0
11	6.0
12	5.0
13	13.0
14	28.0
15	44.0
16	78.0
17	173.0
18	365.0
19	804.0
20	1383.0
21	2086.0
22	3111.0
23	4073.0
24	5230.0
25	6175.0
26	7558.0
27	9531.0
28	11945.0
29	15932.0
30	20955.0
31	27298.0
32	36238.0
33	47504.0
34	64969.0
35	89804.0
36	105046.0
37	135710.0
38	186294.0
39	190683.0
40	32.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.02211835989325	34.74780671330195	20.3030985023888	25.926976424416004
2	21.879223046749352	33.80698254927894	20.440599619970754	23.873194784000955
3	19.808896055115547	31.89779289125388	22.487393161734403	25.80591789189617
4	21.04959186420891	33.32716736393504	18.614547782666023	27.00869298919003
5	17.899192566307292	37.24790177199684	17.122177655965626	27.730728005730242
6	18.19628952514842	40.219590723504574	19.950199520493115	21.633920230853896
7	83.60427630531004	8.670791700194124	4.152677623594288	3.572254370901557
8	84.80643480566405	6.490710453270687	3.346477124767363	5.35637761629789
9	79.31399479797714	10.349271336566357	6.418260312840735	3.918473552615758
10	29.144276490289112	45.403218430493595	12.428538983827691	13.023966095389602
11	18.594200083651653	29.72151399212606	32.617669818504694	19.0666161057176
12	22.7481109011256	26.16652433553972	33.73412134422244	17.351243419112244
13	23.311988802599572	28.52994040590577	28.820974161505237	19.337096629989425
14	18.30727697431771	31.194975146005017	29.960137007840032	20.53761087183724
15	17.39029455863477	34.02042785661655	27.879944465168954	20.70933311957973
16	19.594628618524833	32.24380654065481	28.08804593236137	20.07351890845899
17	17.933413696467824	31.454665223829824	29.14900373349447	21.462917346207878
18	18.453821513683828	32.336809912412406	27.986101904976245	21.223266668927522
19	18.710325840652853	34.23644231787011	27.867612526372366	19.18561931510467
20	19.905085177728928	34.27559622354928	26.4387518844744	19.380566714247397
21	20.248015842430707	31.743951954766448	28.19245634750581	19.81557585529703
22	20.050602055528664	30.866123444762678	26.694228549877042	22.38904594983162
23	18.370375394493585	34.37527939548836	25.537389924600472	21.716955285417587
24	15.988358649776021	36.65216636196501	27.843873544188934	19.515601444070032
25	18.692136230927886	33.8767607696363	25.849593508467418	21.581509490968394
26	20.647262360970238	33.9963805759632	23.963937300312512	21.39241976275405
27	17.27190794618753	32.70625424552684	27.292841412294738	22.728996395990887
28	19.145334981702486	32.30022516064919	27.944995442320952	20.609444415327367
29	17.89826767089755	33.81838959266579	27.3067148434409	20.976627892995765
30	19.59956139404347	32.008266509639974	27.372690716002644	21.01948138031391
31	22.02689801383849	33.51152984894403	24.664288657802057	19.797283479415427
32	20.030048824201018	33.16520790107319	25.033835757073135	21.770907517652656
33	19.397317597779427	32.50308555385306	24.996737174526736	23.102859673840772
34	18.66068978699659	31.773856906348175	27.692498995460817	21.87295431119442
35	18.742183349210702	31.479842932206193	28.38565672198569	21.392316996597412
36	21.369605675980363	32.439370536737364	27.574728979953406	18.61629480732887
37	19.24142133815923	33.34176015817767	26.435771665931888	20.98104683773121
38	20.338347294115714	34.14128085682311	27.544104665275214	17.976267183785964
39	19.8415756929265	32.74078367415729	26.36434918706832	21.05329144584789
40	21.584489709510905	33.240741026202286	25.501935600560284	19.67283366372653
41	20.082973394869708	30.706424837346862	29.00441175110448	20.206190016678946
42	20.89143474914267	31.63132024709095	25.606757080331278	21.870487923435103
43	19.032086677087154	31.66893266042054	27.341038739758066	21.957941922734236
44	19.971472114917226	30.511682970517416	26.6150986092656	22.901746305299753
45	21.88867753316007	32.0561555386334	26.01504702065497	20.040119907551563
46	19.677458140775247	31.872204118250963	28.86670510120925	19.58363263976454
47	19.866547868989592	33.06778558458014	25.895221682014792	21.170444864415472
48	19.770667044846125	31.81753252291942	27.66958214253049	20.742218289703963
49	19.763884478508	31.909199934640725	27.171063516678434	21.15585207017284
50	19.721955886599602	30.46749352316298	26.86029865900442	22.950251931232998
51	18.53336251892182	29.849868921767207	29.38608525685887	22.230683302452103
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	18172.0
1	13667.5
2	9163.0
3	5706.5
4	2250.0
5	2138.5
6	2027.0
7	2208.0
8	2389.0
9	2997.0
10	3605.0
11	4692.5
12	5780.0
13	6273.0
14	6766.0
15	7226.0
16	7686.0
17	7500.5
18	7315.0
19	6960.5
20	6606.0
21	6582.5
22	6559.0
23	6877.0
24	7195.0
25	8204.0
26	9973.5
27	10734.0
28	13692.0
29	16650.0
30	19729.5
31	22809.0
32	29405.0
33	36001.0
34	38436.0
35	40871.0
36	40027.0
37	39183.0
38	41886.0
39	44589.0
40	45556.0
41	46523.0
42	50234.5
43	53946.0
44	65977.5
45	78009.0
46	81424.0
47	84839.0
48	84786.0
49	84733.0
50	81084.0
51	77435.0
52	68785.5
53	60136.0
54	53615.0
55	47094.0
56	40936.0
57	34778.0
58	29932.0
59	25086.0
60	23143.0
61	21200.0
62	19076.5
63	16953.0
64	14139.0
65	11325.0
66	9522.5
67	7720.0
68	6455.0
69	5190.0
70	4394.5
71	3599.0
72	3360.0
73	3121.0
74	2979.0
75	2116.0
76	1395.0
77	1003.0
78	611.0
79	456.5
80	302.0
81	286.5
82	271.0
83	232.0
84	193.0
85	169.5
86	146.0
87	99.5
88	53.0
89	31.5
90	10.0
91	9.0
92	8.0
93	6.0
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	973083.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.685606246388364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.42417207923081	19.373155854869616
2	9.347792954434079	4.802074582007142
3	3.531337437292195	2.7211362881225254
4	1.911409657809744	1.9638286338418
5	1.300015959704558	1.669584902749598
6	0.9504839731582169	1.4648254246826835
7	0.7456853715407391	1.3407366585961065
8	0.6142155015513778	1.2621198018621307
9	0.5338019848326808	1.2339924836357532
>10	4.405556129332864	24.10954677368886
>50	0.7511361440751053	13.616759897618511
>100	0.47416491837556884	20.345276137117065
>500	0.004909386557814346	0.7735219133583943
>1k	0.003682039918360759	1.927762464415126
>5k	0.0012273466394535865	1.7118152382296212
>10k+	4.0911554648452873E-4	1.6838629452051228
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16024	1.6467248939710177	No Hit
AATCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCT	5734	0.589261142163618	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGT	5502	0.5654193938235484	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCC	5054	0.5193801556496208	No Hit
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGCT	3931	0.40397376174488714	Illumina Single End Adapter 1 (95% over 22bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3584	0.3683139053914209	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2987	0.3069625098783968	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1576	0.16195946286185248	No Hit
AATGACTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTT	1526	0.15682115502994093	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCT	1327	0.1363706898589329	No Hit
AACTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG	1315	0.13513749597927413	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1083	0.11129574763920447	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1016	0.10441041514444298	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.49666883503257175	0.0
2	0.0	0.0	0.0	0.6579089347979566	0.0
3	0.0	0.0	0.0	0.9858357406305526	0.0
4	0.0	0.0	0.0	2.3152187429027125	0.0
5	0.0	0.0	0.0	2.511502102081734	0.0
6	0.0	0.0	0.0	3.3801844241447028	0.0
7	0.0	0.0	0.0	4.077041732308549	0.0
8	0.0	0.0	0.0	4.594058266355491	0.0
9	0.0	0.0	0.0	5.916555936132889	0.0
10	0.0	0.0	0.0	7.02509446778949	0.0
11	0.0	0.0	0.0	8.593922615028728	0.0
12	0.0	0.0	0.0	9.12717620182451	0.0
13	0.0	0.0	0.0	9.39046309513166	0.0
14	0.0	0.0	0.0	9.704927534444646	0.0
15	0.0	0.0	0.0	9.912823469323788	0.0
16	0.0	0.0	0.0	10.279287583895721	0.0
17	0.0	0.0	0.0	10.798667739545342	0.0
18	0.0	0.0	0.0	11.505185066433182	0.0
19	0.0	0.0	0.0	11.8062899053832	0.0
20	0.0	0.0	0.0	12.12753691103431	0.0
21	1.0276615663823127E-4	0.0	0.0	12.479613763676891	0.0
22	1.0276615663823127E-4	0.0	0.0	12.862931527937494	0.0
23	1.0276615663823127E-4	0.0	0.0	13.254881649355708	0.0
24	1.0276615663823127E-4	0.0	0.0	13.522176422771746	0.0
25	1.0276615663823127E-4	0.0	0.0	13.765115617064525	0.0
26	1.0276615663823127E-4	0.0	0.0	14.000039051139522	0.0
27	1.0276615663823127E-4	0.0	0.0	14.253871458035954	0.0
28	1.0276615663823127E-4	0.0	0.0	14.523940917681225	0.0
29	1.0276615663823127E-4	0.0	0.0	14.807678276159383	0.0
30	1.0276615663823127E-4	0.0	0.0	15.180205593972971	0.0
31	1.0276615663823127E-4	0.0	0.0	15.451713779811177	0.0
32	1.0276615663823127E-4	0.0	0.0	15.706882146743906	0.0
33	1.0276615663823127E-4	0.0	0.0	15.992983126824742	0.0
34	1.0276615663823127E-4	0.0	0.0	16.258222577108018	0.0
35	1.0276615663823127E-4	0.0	0.0	16.57926405044585	0.0
36	1.0276615663823127E-4	0.0	0.0	16.85817139956201	0.0
37	1.0276615663823127E-4	0.0	0.0	17.129165754617027	0.0
38	1.0276615663823127E-4	0.0	0.0	17.420507808686413	0.0
39	1.0276615663823127E-4	0.0	0.0	17.705170062574314	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGTT	25	3.8903625E-5	45.000004	35
ACACGTA	25	3.8903625E-5	45.000004	32
CGACGTG	35	1.2116652E-7	45.000004	1
CGCGGAA	25	3.8903625E-5	45.000004	4
GGCGTAC	35	1.2116652E-7	45.000004	8
TAGCGAT	25	3.8903625E-5	45.000004	45
ATATGCG	25	3.8903625E-5	45.000004	1
TAGCCGA	35	1.2116652E-7	45.000004	41
GTTACGT	25	3.8903625E-5	45.000004	45
CTAAACG	35	1.2116652E-7	45.000004	1
ACTACGC	25	3.8903625E-5	45.000004	13
ACTCGTC	25	3.8903625E-5	45.000004	18
CATACGT	25	3.8903625E-5	45.000004	23
CGTAATC	25	3.8903625E-5	45.000004	23
GTATACG	70	0.0	45.000004	1
TCGAGCA	35	1.2116652E-7	45.000004	31
TATCGTG	50	2.1827873E-11	45.000004	1
ATACCCG	35	1.2116652E-7	45.000004	1
TCTATCG	25	3.8903625E-5	45.000004	26
CTAGCGA	25	3.8903625E-5	45.000004	44
>>END_MODULE