SRR3553248_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553248_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	776206
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGCT	2858	0.3682012249325566	RNA PCR Primer, Index 30 (96% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1814	0.23370084745544356	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	960	0.12367850802493152	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	815	0.10499790004199916	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCCGT	30	2.1645865E-6	45.000004	19
TTATCCG	30	2.1645865E-6	45.000004	1
TTGCGAA	30	2.1645865E-6	45.000004	25
TATACCG	30	2.1645865E-6	45.000004	1
CGATCGT	45	3.8380676E-10	45.000004	27
TATCGCG	45	3.8380676E-10	45.000004	1
TAGTCCG	30	2.1645865E-6	45.000004	1
TACGTAG	45	3.8380676E-10	45.000004	1
GTCGCTC	20	7.03187E-4	45.0	35
GTCGATT	20	7.03187E-4	45.0	28
TTAGCGA	20	7.03187E-4	45.0	20
TAGCGTC	20	7.03187E-4	45.0	28
ATTACGA	25	3.889631E-5	45.0	23
ATTACCG	25	3.889631E-5	45.0	1
TCTAGCG	40	6.8102963E-9	45.0	1
ATCCGTC	20	7.03187E-4	45.0	11
ACGATAG	20	7.03187E-4	45.0	1
ACGGCGT	25	3.889631E-5	45.0	17
CATAGCG	35	1.2112469E-7	45.0	1
CGTAGCA	25	3.889631E-5	45.0	19