##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553248_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	776206
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.897139419174806	33.0	31.0	34.0	30.0	34.0
2	32.103074441578656	34.0	31.0	34.0	30.0	34.0
3	32.28122044921065	34.0	31.0	34.0	30.0	34.0
4	35.9201500632564	37.0	35.0	37.0	35.0	37.0
5	35.69660373663692	37.0	35.0	37.0	33.0	37.0
6	35.0573494665076	37.0	35.0	37.0	33.0	37.0
7	35.48074609059966	37.0	35.0	37.0	35.0	37.0
8	35.86923832075506	37.0	35.0	37.0	35.0	37.0
9	37.566607318160386	39.0	37.0	39.0	35.0	39.0
10	37.095411527352276	39.0	37.0	39.0	33.0	39.0
11	37.0911149875162	39.0	37.0	39.0	33.0	39.0
12	36.975773183922826	39.0	37.0	39.0	33.0	39.0
13	36.93951992125802	39.0	37.0	39.0	33.0	39.0
14	38.164610940910016	40.0	37.0	41.0	33.0	41.0
15	38.16950397188375	40.0	37.0	41.0	33.0	41.0
16	38.09800491106742	40.0	37.0	41.0	33.0	41.0
17	38.10458563834858	40.0	37.0	41.0	33.0	41.0
18	38.068774268686404	40.0	37.0	41.0	33.0	41.0
19	38.10499661172421	40.0	37.0	41.0	34.0	41.0
20	38.004001515061724	40.0	37.0	41.0	33.0	41.0
21	38.009751277367094	40.0	37.0	41.0	33.0	41.0
22	37.97958273963355	40.0	37.0	41.0	33.0	41.0
23	37.87870101493676	40.0	37.0	41.0	33.0	41.0
24	37.71827324189712	40.0	36.0	41.0	33.0	41.0
25	37.758855252342805	40.0	37.0	41.0	33.0	41.0
26	37.70544546164292	40.0	36.0	41.0	33.0	41.0
27	37.63720326820457	40.0	36.0	41.0	33.0	41.0
28	37.592564345032116	40.0	36.0	41.0	33.0	41.0
29	37.415125623867894	39.0	36.0	41.0	32.0	41.0
30	37.45575916702525	39.0	36.0	41.0	32.0	41.0
31	37.41636112063035	39.0	36.0	41.0	32.0	41.0
32	37.39332213355733	39.0	36.0	41.0	32.0	41.0
33	37.128115474500326	39.0	36.0	41.0	31.0	41.0
34	37.14028363604507	39.0	35.0	41.0	31.0	41.0
35	37.08463346070502	39.0	35.0	41.0	31.0	41.0
36	37.05116167615298	39.0	35.0	41.0	31.0	41.0
37	37.01999726876628	39.0	35.0	41.0	31.0	41.0
38	36.83382504129059	39.0	35.0	41.0	31.0	41.0
39	36.78257833616334	39.0	35.0	41.0	31.0	41.0
40	36.664148434822714	39.0	35.0	41.0	31.0	41.0
41	36.63840011543327	39.0	35.0	40.0	31.0	41.0
42	36.604856185084884	39.0	35.0	40.0	31.0	41.0
43	36.560775103516335	39.0	35.0	40.0	31.0	41.0
44	36.47469357361319	39.0	35.0	40.0	31.0	41.0
45	36.26978920544288	38.0	35.0	40.0	30.0	41.0
46	36.1128643684795	38.0	35.0	40.0	30.0	41.0
47	35.97615452598924	38.0	35.0	40.0	30.0	41.0
48	35.90014892953675	38.0	35.0	40.0	29.0	41.0
49	35.80009559318016	38.0	35.0	40.0	29.0	41.0
50	35.71287647866674	38.0	34.0	40.0	29.0	41.0
51	34.5186857612541	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	2.0
11	5.0
12	3.0
13	10.0
14	23.0
15	39.0
16	51.0
17	120.0
18	248.0
19	481.0
20	815.0
21	1201.0
22	1732.0
23	2349.0
24	3051.0
25	3724.0
26	4472.0
27	5736.0
28	7454.0
29	9402.0
30	12530.0
31	16537.0
32	21912.0
33	30409.0
34	47768.0
35	59961.0
36	72266.0
37	106809.0
38	165316.0
39	201725.0
40	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.280956344063302	32.50425789030232	22.083828262085063	25.13095750354932
2	24.02996111856904	30.410999141980348	20.660237102006427	24.898802637444184
3	20.788167058744715	31.090071450104737	20.19476788378343	27.926993607367116
4	20.3388018129208	31.711684784709217	19.658698850562867	28.290814551807124
5	18.62508148610034	33.39616029765294	17.611948374529444	30.366809841717274
6	20.322569008742526	37.943793271373835	18.6310077479432	23.102629971940438
7	86.43221000610663	5.073524296385238	4.559871992744194	3.9343937047639415
8	88.12325593978917	4.358250258307717	3.6391112668544174	3.879382535048685
9	80.79878279735019	8.233381344643046	6.515538400888424	4.452297457118342
10	36.74552889310312	35.68253788298467	12.321729025542188	15.250204198370021
11	26.937436711388475	29.319665140439522	25.14938044797386	18.59351770019814
12	28.098339873693323	23.916975648217097	28.72910026462047	19.255584213469103
13	26.55132786914814	25.187901149952463	25.989363648309855	22.271407332589543
14	21.747061991275512	27.267890225017588	27.473119249271456	23.511928534435448
15	20.718211402643114	29.728706039376146	27.444776257849075	22.108306300131666
16	23.563976573229272	27.826762483155242	26.399177537921638	22.21008340569385
17	22.236880415765917	27.676673460395822	27.716353648387155	22.370092475451102
18	23.357072735845897	27.36155092849063	26.111109679646898	23.170266656016572
19	23.07480230763483	29.93329090473405	25.970554208547732	21.021352579083388
20	25.991811452114515	28.29107221536551	24.828460486005007	20.888655846514972
21	25.072725539354245	27.929827906509352	25.294702694903158	21.702743859233244
22	23.831946673949954	26.054681360360522	25.971713694560467	24.141658271129057
23	23.878326114459306	28.100401182160407	24.81506198096897	23.20621072241132
24	22.00833283947818	27.627459720744234	26.504175437963635	23.860032001813952
25	22.88773856424712	28.67408909490522	24.452915849658467	23.985256491189194
26	24.287367013395926	28.400579227679252	24.29097430321332	23.021079455711497
27	21.898696995385244	27.65116476811568	26.241487440189847	24.20865079630923
28	22.504335189369833	28.7356706853593	26.596161328307176	22.163832796963693
29	23.237001517638358	28.443866705487974	25.494520784430936	22.82461099244273
30	23.254522639608556	27.948379682713092	24.23080986233036	24.566287815347987
31	24.188166543417598	29.067283685001144	23.44506484103447	23.29948493054679
32	24.54631888957313	27.376624246656174	24.263661966024483	23.813394897746218
33	23.4305068499857	28.220214736809556	24.393524399450662	23.95575401375408
34	22.48475275893255	28.366567637972395	25.669989667691308	23.478689935403747
35	23.02507324086647	27.666624581618798	25.91876383331229	23.38953834420244
36	23.124016047286418	30.511874425088187	24.60751398468963	21.756595542935766
37	23.554700685127404	28.930464335498566	23.897779713117394	23.617055266256635
38	24.875870580747893	27.811431501431322	24.671156883610795	22.641541034209993
39	24.229650376317625	26.935246571142198	24.69795389368286	24.137149158857312
40	23.391728484448716	28.251649690932563	24.585226086889307	23.771395737729417
41	21.39277459849576	27.143825221655078	26.90007549542261	24.563324684426558
42	22.774495430336792	27.5423019146979	24.490148233845137	25.193054421120166
43	22.429741589217294	26.600412777020534	25.80590719473954	25.163938439022633
44	23.78968985037477	25.23711488960405	24.778602587457456	26.194592672563726
45	24.73995305369966	26.078257575952772	25.639456536022653	23.54233283432491
46	21.294733614530166	26.967583347719547	27.157996717366267	24.579686320384024
47	22.014645596658617	27.60607364539826	26.260683375289553	24.118597382653572
48	22.98938683803011	26.110980847867705	27.344545133637205	23.55508718046498
49	22.492869161021687	26.655295114956594	26.390416976936535	24.46141874708518
50	22.020958353839056	25.728865790782347	26.49193641894033	25.758239436438263
51	22.236365088649144	24.970819602012867	27.440395977356523	25.35241933198146
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2252.0
1	1802.0
2	1352.0
3	951.5
4	551.0
5	519.5
6	488.0
7	537.0
8	586.0
9	667.5
10	749.0
11	839.0
12	929.0
13	1014.5
14	1100.0
15	1288.0
16	1476.0
17	1445.5
18	1415.0
19	1559.5
20	1704.0
21	2030.0
22	2356.0
23	3050.5
24	3745.0
25	4272.0
26	5724.0
27	6649.0
28	7393.0
29	8137.0
30	9850.0
31	11563.0
32	15447.0
33	19331.0
34	20402.5
35	21474.0
36	23542.0
37	25610.0
38	26846.5
39	28083.0
40	31035.0
41	33987.0
42	35857.5
43	37728.0
44	42920.0
45	48112.0
46	54555.0
47	60998.0
48	64508.0
49	68018.0
50	66808.0
51	65598.0
52	60175.0
53	54752.0
54	50681.5
55	46611.0
56	43751.5
57	40892.0
58	39699.0
59	38506.0
60	37513.5
61	36521.0
62	32973.0
63	29425.0
64	25797.0
65	22169.0
66	18614.5
67	15060.0
68	12696.5
69	10333.0
70	9409.0
71	8485.0
72	6813.5
73	5142.0
74	4426.5
75	3201.0
76	2691.0
77	2135.0
78	1579.0
79	1173.5
80	768.0
81	505.0
82	242.0
83	262.5
84	283.0
85	232.0
86	181.0
87	109.0
88	37.0
89	26.5
90	16.0
91	10.0
92	4.0
93	5.0
94	6.0
95	4.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	776206.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.17420654537097
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.1818334283128	18.926430031840223
2	10.294328088818661	5.183030831074699
3	3.6101284032343743	2.7264635423499732
4	1.7203582489809268	1.7323461556751436
5	0.9633830912585628	1.2126202460830515
6	0.6935371439074227	1.047554838456728
7	0.48678027411502794	0.8578015013948806
8	0.3793132363847979	0.7639127806515221
9	0.32897431620732603	0.7453500645892865
>10	4.412872947087146	27.287205842591096
>50	1.651426799420528	29.077445216680765
>100	0.27288979971465743	9.269348569127642
>500	0.00313066691833259	0.5568071730037988
>1k	0.0010435556394441966	0.6136832064811861
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGCT	2858	0.3682012249325566	RNA PCR Primer, Index 30 (96% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1814	0.23370084745544356	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	960	0.12367850802493152	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	815	0.10499790004199916	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4207645908431525	0.0
2	0.0	0.0	0.0	0.6178772130078871	0.0
3	0.0	0.0	0.0	0.8621422663571269	0.0
4	0.0	0.0	0.0	1.9284829027345833	0.0
5	0.0	0.0	0.0	2.0909397762964987	0.0
6	0.0	0.0	0.0	2.9225489109849705	0.0
7	0.0	0.0	0.0	3.5397819650968945	0.0
8	0.0	0.0	0.0	4.001515061723305	0.0
9	0.0	0.0	0.0	4.71601610912567	0.0
10	0.0	0.0	0.0	5.791117306488226	0.0
11	0.0	0.0	0.0	6.873561915264762	0.0
12	0.0	0.0	0.0	7.401901041733766	0.0
13	1.28831779192637E-4	0.0	0.0	7.6258106739705696	0.0
14	1.28831779192637E-4	0.0	0.0	7.885922036160504	0.0
15	1.28831779192637E-4	0.0	0.0	8.116659752694517	0.0
16	1.28831779192637E-4	0.0	0.0	8.587539905643604	0.0
17	1.28831779192637E-4	0.0	0.0	9.175914641216378	0.0
18	1.28831779192637E-4	0.0	0.0	9.909611623718446	0.0
19	1.28831779192637E-4	0.0	0.0	10.259518736005647	0.0
20	1.28831779192637E-4	0.0	0.0	10.680154495069608	0.0
21	1.28831779192637E-4	0.0	0.0	11.11019497401463	0.0
22	1.28831779192637E-4	0.0	0.0	11.586099566352232	0.0
23	1.28831779192637E-4	0.0	0.0	12.090347150112212	0.0
24	1.28831779192637E-4	0.0	0.0	12.437548795036369	0.0
25	1.28831779192637E-4	0.0	0.0	12.76439501884809	0.0
26	1.28831779192637E-4	0.0	0.0	13.049113250863817	0.0
27	1.28831779192637E-4	0.0	0.0	13.362303306081118	0.0
28	1.28831779192637E-4	0.0	0.0	13.692627987931038	0.0
29	2.57663558385274E-4	0.0	0.0	14.026688791377547	0.0
30	2.57663558385274E-4	0.0	0.0	14.487906560887188	0.0
31	3.8649533757791104E-4	0.0	0.0	14.798906475858212	0.0
32	3.8649533757791104E-4	0.0	0.0	15.127814008137015	0.0
33	3.8649533757791104E-4	0.0	0.0	15.44074639979593	0.0
34	3.8649533757791104E-4	0.0	0.0	15.76900977317877	0.0
35	3.8649533757791104E-4	0.0	0.0	16.122524175283367	0.0
36	3.8649533757791104E-4	0.0	0.0	16.453235352470866	0.0
37	3.8649533757791104E-4	0.0	0.0	16.785363679229484	0.0
38	3.8649533757791104E-4	0.0	0.0	17.121614622922266	0.0
39	3.8649533757791104E-4	0.0	0.0	17.462117015328406	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCCGT	30	2.1645865E-6	45.000004	19
TTATCCG	30	2.1645865E-6	45.000004	1
TTGCGAA	30	2.1645865E-6	45.000004	25
TATACCG	30	2.1645865E-6	45.000004	1
CGATCGT	45	3.8380676E-10	45.000004	27
TATCGCG	45	3.8380676E-10	45.000004	1
TAGTCCG	30	2.1645865E-6	45.000004	1
TACGTAG	45	3.8380676E-10	45.000004	1
GTCGCTC	20	7.03187E-4	45.0	35
GTCGATT	20	7.03187E-4	45.0	28
TTAGCGA	20	7.03187E-4	45.0	20
TAGCGTC	20	7.03187E-4	45.0	28
ATTACGA	25	3.889631E-5	45.0	23
ATTACCG	25	3.889631E-5	45.0	1
TCTAGCG	40	6.8102963E-9	45.0	1
ATCCGTC	20	7.03187E-4	45.0	11
ACGATAG	20	7.03187E-4	45.0	1
ACGGCGT	25	3.889631E-5	45.0	17
CATAGCG	35	1.2112469E-7	45.0	1
CGTAGCA	25	3.889631E-5	45.0	19
>>END_MODULE