##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553246_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	423603
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.730079815298758	31.0	31.0	34.0	30.0	34.0
2	31.91997932025977	33.0	31.0	34.0	30.0	34.0
3	32.09374579500145	34.0	31.0	34.0	30.0	34.0
4	35.770046482201494	37.0	35.0	37.0	35.0	37.0
5	35.534939554252446	37.0	35.0	37.0	33.0	37.0
6	34.92965583341006	37.0	35.0	37.0	32.0	37.0
7	35.327020819021584	37.0	35.0	37.0	33.0	37.0
8	35.709633784463286	37.0	35.0	37.0	35.0	37.0
9	37.36773346742115	39.0	37.0	39.0	34.0	39.0
10	36.92631544158091	39.0	37.0	39.0	33.0	39.0
11	36.92242972783479	39.0	37.0	39.0	33.0	39.0
12	36.82255319249391	39.0	37.0	39.0	33.0	39.0
13	36.772631449730056	39.0	37.0	39.0	33.0	39.0
14	37.939761994131295	40.0	37.0	41.0	33.0	41.0
15	37.981114392485416	40.0	37.0	41.0	33.0	41.0
16	37.877219944145814	40.0	37.0	41.0	33.0	41.0
17	37.86105858551521	40.0	37.0	41.0	33.0	41.0
18	37.816106118228625	40.0	37.0	41.0	33.0	41.0
19	37.82428358628244	40.0	36.0	41.0	33.0	41.0
20	37.74271428672601	40.0	36.0	41.0	33.0	41.0
21	37.71858792312614	39.0	36.0	41.0	33.0	41.0
22	37.70643975609238	39.0	36.0	41.0	33.0	41.0
23	37.59046087964438	39.0	36.0	41.0	33.0	41.0
24	37.43825468658154	39.0	36.0	41.0	32.0	41.0
25	37.45694907732004	39.0	36.0	41.0	32.0	41.0
26	37.43631182970848	39.0	36.0	41.0	32.0	41.0
27	37.35509663529295	39.0	36.0	41.0	32.0	41.0
28	37.297242937372964	39.0	36.0	41.0	32.0	41.0
29	37.03559228806217	39.0	35.0	41.0	31.0	41.0
30	37.029577222068774	39.0	35.0	41.0	31.0	41.0
31	37.02397055733788	39.0	35.0	41.0	31.0	41.0
32	36.904103606442824	39.0	35.0	41.0	31.0	41.0
33	36.71931737971639	39.0	35.0	41.0	31.0	41.0
34	36.681307733892346	39.0	35.0	41.0	30.0	41.0
35	36.57265883386095	39.0	35.0	40.0	30.0	41.0
36	36.558659877290765	39.0	35.0	40.0	30.0	41.0
37	36.448594556695774	39.0	35.0	40.0	30.0	41.0
38	36.25287592391933	39.0	35.0	40.0	30.0	41.0
39	36.19500334039183	38.0	35.0	40.0	30.0	41.0
40	36.13703632882675	38.0	35.0	40.0	30.0	41.0
41	36.12499911473715	38.0	35.0	40.0	30.0	41.0
42	36.04545529658666	38.0	35.0	40.0	29.0	41.0
43	35.96589731423054	38.0	35.0	40.0	29.0	41.0
44	35.83642939261526	38.0	35.0	40.0	29.0	41.0
45	35.654860801269116	38.0	34.0	40.0	28.0	41.0
46	35.43856865980647	38.0	34.0	40.0	28.0	41.0
47	35.35441911412337	38.0	34.0	40.0	28.0	41.0
48	35.23454744182643	37.0	34.0	40.0	27.0	41.0
49	35.08257731885752	37.0	34.0	40.0	27.0	41.0
50	34.995970283496575	37.0	34.0	40.0	27.0	41.0
51	33.69583076607106	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	3.0
11	5.0
12	1.0
13	6.0
14	16.0
15	26.0
16	47.0
17	93.0
18	199.0
19	353.0
20	633.0
21	990.0
22	1401.0
23	1729.0
24	2236.0
25	2656.0
26	3120.0
27	3912.0
28	4752.0
29	6377.0
30	7920.0
31	10480.0
32	14011.0
33	18846.0
34	28876.0
35	34959.0
36	40864.0
37	59453.0
38	84748.0
39	94857.0
40	32.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.025575833976625	30.47995410797374	19.707603581655462	27.786866476394174
2	23.524384860352736	30.958704258468423	19.869311595999083	25.647599285179755
3	20.288099942634968	31.916204559457796	21.237101720242773	26.558593777664463
4	21.159906799526915	31.763703278777534	20.021104666397544	27.055285255298
5	18.75694931339013	34.727563308097444	18.05086366243865	28.464623716073778
6	21.74677705304259	36.25729751677868	19.280080641544085	22.71584478863464
7	82.55890539018846	6.649386335790823	5.88640779220166	4.9053004818190615
8	84.40284889389358	5.155062641199424	4.702516271131224	5.739572193775776
9	77.35780908067224	8.471847460948105	8.751590522257867	5.418752936121794
10	35.28610515034124	34.197113807031585	13.723462770565837	16.79331827206134
11	25.526023186804625	28.209195874439036	27.058590236613057	19.20619070214328
12	26.221013543341286	23.5175388276287	30.704928907491215	19.556518721538797
13	25.3657315930246	24.712525643113953	28.129876322877788	21.791866440983657
14	19.81430726411286	26.85438960536162	30.127029317544967	23.20427381298055
15	18.16512158790188	28.842335866365442	29.640960994138382	23.3515815515943
16	21.677136375332566	27.624686321862686	27.501693802923967	23.196483499880785
17	21.338375790539725	27.520107270250683	27.71344867718123	23.42806826202836
18	22.621180680967793	26.692681591018	26.441975151261914	24.24416257675229
19	22.389123778632353	30.51725318281504	26.34258964171642	20.75103339683619
20	24.33410528254049	28.297486089569716	25.695993654435874	21.672414973453918
21	23.798462239408124	29.07368455841909	25.507373649383975	21.62047955278881
22	22.547526811660916	26.93866662889545	25.943395112876917	24.570411446566716
23	22.46513834887855	28.18983812673659	24.603461259717236	24.741562264667625
24	20.4989105365165	28.426852501044607	26.55103953465863	24.523197427780257
25	23.30814465431076	28.544887548010756	24.251008609476326	23.895959188202163
26	23.838122015188752	27.68299563506396	24.12471110922255	24.354171240524735
27	20.633234419963976	27.37657665313985	25.687731201148246	26.302457725747928
28	24.67286586733333	26.833379367001648	26.61383417964462	21.8799205860204
29	24.805301190029343	28.91410117492086	23.148089130624665	23.132508504425132
30	24.227165529989165	27.998621350651437	25.44623149505551	22.327981624303888
31	27.14640831155587	28.26065915491628	21.396212963553136	23.196719569974718
32	24.58315923163906	29.304561110284865	22.214667979216387	23.897611678859686
33	24.984478391323954	26.570161212267145	24.474566988430205	23.970793407978697
34	23.444121028415758	27.146172241461937	24.586700283048042	24.823006447074263
35	24.84614131627963	27.926619972002086	25.45945142031572	21.767787291402563
36	24.69552859635083	28.98185329187942	24.792789475050935	21.529828636718815
37	22.905173003968336	30.79534375346728	22.572078101429877	23.727405141134504
38	24.44128110518575	28.58785230510643	25.732348448901448	21.238518140806367
39	22.632748115570475	27.53639610673201	25.556240158827958	24.274615618869554
40	23.63935099609776	28.035920425492737	24.94505468563726	23.379673892772242
41	21.38842265045337	25.525078906428895	27.311657377308467	25.774841065809262
42	24.758323241336818	27.310240956744874	24.245815067409815	23.68562073450849
43	22.657535475433367	25.39382393420254	26.538291749586286	25.410348840777804
44	23.64926594004292	24.419798726637914	25.698354355375198	26.23258097794397
45	25.63367114963775	24.762808573121532	26.875635913815533	22.72788436342519
46	22.258577016687795	26.088578220645275	28.353906842019533	23.298937920647397
47	22.388415568350556	25.986359869972592	26.334091118334857	25.291133443341995
48	22.278879044765972	24.743686895513015	29.29983970840622	23.67759435131479
49	22.890536658144537	25.716767822701918	27.046786731916438	24.345908787237107
50	22.139833759439853	24.021784548268073	26.275073594851783	27.56330809744029
51	22.205933385740895	23.371411439484614	29.26372098403458	25.158934190739913
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1606.0
1	1375.0
2	1144.0
3	791.0
4	438.0
5	430.5
6	423.0
7	459.5
8	496.0
9	584.0
10	672.0
11	810.0
12	948.0
13	921.0
14	894.0
15	973.5
16	1053.0
17	1092.5
18	1132.0
19	1183.0
20	1234.0
21	1268.5
22	1303.0
23	1526.5
24	1750.0
25	2181.5
26	2941.0
27	3269.0
28	4325.5
29	5382.0
30	5783.5
31	6185.0
32	7099.0
33	8013.0
34	9541.0
35	11069.0
36	12113.5
37	13158.0
38	14033.0
39	14908.0
40	15406.0
41	15904.0
42	17772.5
43	19641.0
44	23922.5
45	28204.0
46	31097.0
47	33990.0
48	37614.0
49	41238.0
50	40090.5
51	38943.0
52	34763.5
53	30584.0
54	27098.5
55	23613.0
56	22362.0
57	21111.0
58	20454.5
59	19798.0
60	19069.0
61	18340.0
62	15427.0
63	12514.0
64	11459.0
65	10404.0
66	9243.0
67	8082.0
68	7822.5
69	7563.0
70	6191.0
71	4819.0
72	4212.0
73	3605.0
74	3365.0
75	2534.0
76	1943.0
77	1526.0
78	1109.0
79	879.0
80	649.0
81	557.5
82	466.0
83	300.0
84	134.0
85	113.0
86	92.0
87	62.0
88	32.0
89	18.0
90	4.0
91	4.0
92	4.0
93	2.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	423603.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.709265693753913
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.5850826658123	24.28456734428349
2	9.904683157805154	6.281404597265864
3	3.4014164583657527	3.235692546402813
4	1.7511575611685684	2.22111681514881
5	1.02281052329369	1.6216285318743546
6	0.639664499459202	1.2169974940928368
7	0.4785100127351264	1.0621240791657782
8	0.42208860655451647	1.0707295817234819
9	0.3379509068545831	0.9644557587207352
>10	4.526920443472672	34.41395158279721
>50	0.8456052303737723	17.493560159576255
>100	0.07885306322313153	4.003692071587427
>500	0.001501963109012029	0.2833755763509807
>1k	0.0037549077725300725	1.8467038610099622
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCTGCT	2866	0.6765768892099442	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1323	0.3123207342724202	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCC	1211	0.28588088375200366	No Hit
AATCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCT	1188	0.28045127159156097	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGT	1167	0.2754937996189829	No Hit
CCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCTGC	648	0.15297342086812415	No Hit
AAACTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCT	542	0.12794999091130138	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTT	468	0.1104808039603119	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7521193192682771	0.0
2	0.0	0.0	0.0	1.0840338713370774	0.0
3	0.0	0.0	0.0	1.4770905777343408	0.0
4	0.0	0.0	0.0	3.2190518008607114	0.0
5	0.0	0.0	0.0	3.47967318456196	0.0
6	0.0	0.0	0.0	4.823384159224557	0.0
7	0.0	0.0	0.0	5.866341834217415	0.0
8	0.0	0.0	0.0	6.8108582800405095	0.0
9	0.0	0.0	0.0	8.221613161379876	0.0
10	0.0	0.0	0.0	10.092232585699346	0.0
11	0.0	0.0	0.0	11.799255434923738	0.0
12	0.0	0.0	0.0	12.610864417862953	0.0
13	0.0	0.0	0.0	12.970871311109695	0.0
14	0.0	0.0	0.0	13.370774050230995	0.0
15	0.0	0.0	0.0	13.739751607047165	0.0
16	0.0	0.0	0.0	14.361560234464818	0.0
17	0.0	0.0	0.0	15.136578352844527	0.0
18	0.0	0.0	0.0	16.230055027938896	0.0
19	0.0	0.0	0.0	16.676463575564856	0.0
20	0.0	0.0	0.0	17.25483530569897	0.0
21	0.0	0.0	0.0	17.79189476939493	0.0
22	0.0	0.0	0.0	18.33650847609672	0.0
23	0.0	0.0	0.0	18.883010743549974	0.0
24	0.0	0.0	0.0	19.28669060417419	0.0
25	0.0	0.0	0.0	19.64008753479083	0.0
26	0.0	0.0	0.0	19.981917030804787	0.0
27	0.0	0.0	0.0	20.414161372794812	0.0
28	0.0	0.0	0.0	20.762128691251007	0.0
29	0.0	0.0	0.0	21.158962519151185	0.0
30	0.0	0.0	0.0	21.708297627731625	0.0
31	0.0	0.0	0.0	22.05083533402738	0.0
32	0.0	0.0	0.0	22.39738623191998	0.0
33	0.0	0.0	0.0	22.759517756012116	0.0
34	0.0	0.0	0.0	23.11149826606516	0.0
35	0.0	0.0	0.0	23.47646263128448	0.0
36	0.0	0.0	0.0	23.832456332934374	0.0
37	0.0	0.0	0.0	24.16814800650609	0.0
38	0.0	0.0	0.0	24.515407114680492	0.0
39	0.0	0.0	0.0	24.875886148115097	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGA	20	7.028236E-4	45.000004	43
GGTCAAT	20	7.028236E-4	45.000004	9
TCTAGCG	20	7.028236E-4	45.000004	1
ATCGTTA	20	7.028236E-4	45.000004	12
GTTACGA	20	7.028236E-4	45.000004	15
ACGATCT	20	7.028236E-4	45.000004	32
TACGCCC	20	7.028236E-4	45.000004	28
ACGGCAT	20	7.028236E-4	45.000004	5
CCATACT	20	7.028236E-4	45.000004	18
TACTCGT	20	7.028236E-4	45.000004	21
ACGTACT	20	7.028236E-4	45.000004	14
GCGGAAT	20	7.028236E-4	45.000004	5
TCGAGGT	20	7.028236E-4	45.000004	16
TCATACG	20	7.028236E-4	45.000004	1
ATAGTCG	20	7.028236E-4	45.000004	27
GTCCAAT	20	7.028236E-4	45.000004	44
CGATCTT	20	7.028236E-4	45.000004	33
TAATGCG	20	7.028236E-4	45.000004	1
AATCGCG	20	7.028236E-4	45.000004	1
GTACGAC	20	7.028236E-4	45.000004	1
>>END_MODULE