##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3553245_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 430626 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.746884767756708 31.0 31.0 34.0 30.0 34.0 2 31.94211218087157 33.0 31.0 34.0 30.0 34.0 3 32.09019427531083 34.0 31.0 34.0 30.0 34.0 4 35.7648725344034 37.0 35.0 37.0 35.0 37.0 5 35.54210614314974 37.0 35.0 37.0 33.0 37.0 6 35.56369099868564 37.0 35.0 37.0 33.0 37.0 7 35.648969175107865 37.0 35.0 37.0 35.0 37.0 8 35.70626251085629 37.0 35.0 37.0 35.0 37.0 9 37.405340132736995 39.0 37.0 39.0 35.0 39.0 10 36.93863816861964 39.0 37.0 39.0 33.0 39.0 11 36.92224575385602 39.0 37.0 39.0 33.0 39.0 12 36.86406765963969 39.0 37.0 39.0 33.0 39.0 13 36.811571990543996 39.0 37.0 39.0 33.0 39.0 14 37.975537937792886 40.0 37.0 41.0 33.0 41.0 15 38.00129578799236 40.0 37.0 41.0 33.0 41.0 16 37.9140112301626 40.0 37.0 41.0 33.0 41.0 17 37.86769029273662 40.0 37.0 41.0 33.0 41.0 18 37.79683530488173 40.0 37.0 41.0 33.0 41.0 19 37.83276439416106 40.0 37.0 41.0 33.0 41.0 20 37.75709780644922 40.0 36.0 41.0 33.0 41.0 21 37.72424795530228 39.0 36.0 41.0 33.0 41.0 22 37.705809681719174 39.0 36.0 41.0 33.0 41.0 23 37.55093747242387 39.0 36.0 41.0 32.0 41.0 24 37.41710904590062 39.0 36.0 41.0 32.0 41.0 25 37.43278157844626 39.0 36.0 41.0 32.0 41.0 26 37.390157584539715 39.0 36.0 41.0 32.0 41.0 27 37.34764041186552 39.0 36.0 41.0 32.0 41.0 28 37.2736829638712 39.0 36.0 41.0 32.0 41.0 29 37.067987069986486 39.0 35.0 41.0 31.0 41.0 30 37.047591181210606 39.0 35.0 41.0 31.0 41.0 31 37.01879124808999 39.0 35.0 41.0 31.0 41.0 32 36.87328447423054 39.0 35.0 41.0 31.0 41.0 33 36.84969555948781 39.0 35.0 41.0 31.0 41.0 34 36.76767543065212 39.0 35.0 41.0 31.0 41.0 35 36.63781100072917 39.0 35.0 40.0 30.0 41.0 36 36.558640212156256 39.0 35.0 40.0 30.0 41.0 37 36.44673568247156 39.0 35.0 40.0 30.0 41.0 38 36.36374022934054 39.0 35.0 40.0 30.0 41.0 39 36.26128705651772 39.0 35.0 40.0 30.0 41.0 40 36.171652431576355 38.0 35.0 40.0 30.0 41.0 41 36.09918351423277 38.0 35.0 40.0 29.0 41.0 42 36.01978282779024 38.0 35.0 40.0 29.0 41.0 43 35.93614412506444 38.0 35.0 40.0 29.0 41.0 44 35.82484104536187 38.0 35.0 40.0 29.0 41.0 45 35.660775243482746 38.0 34.0 40.0 28.0 41.0 46 35.42532731418911 38.0 34.0 40.0 28.0 41.0 47 35.320881693162974 38.0 34.0 40.0 28.0 41.0 48 35.21749964005889 38.0 34.0 40.0 27.0 41.0 49 35.00417067246288 37.0 34.0 40.0 26.0 41.0 50 34.93954382689387 37.0 34.0 40.0 27.0 41.0 51 33.56952436685198 36.0 32.0 39.0 23.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 4.0 10 0.0 11 10.0 12 2.0 13 7.0 14 8.0 15 26.0 16 43.0 17 100.0 18 196.0 19 347.0 20 639.0 21 948.0 22 1394.0 23 1795.0 24 2218.0 25 2702.0 26 3238.0 27 3884.0 28 5043.0 29 6243.0 30 8198.0 31 10482.0 32 14013.0 33 18844.0 34 29045.0 35 34944.0 36 41535.0 37 59753.0 38 87887.0 39 97067.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.914608035743314 30.628898394430433 19.749388100114718 27.707105469711536 2 23.471875827284 31.147910251587223 19.99856023556404 25.381653685564736 3 20.30091076711578 31.669476529517492 21.378876333523753 26.650736369842974 4 21.187526995583177 31.669708749587812 20.07612173904966 27.066642515779353 5 18.709738845308923 34.787727633723925 18.073223632572116 28.429309888395032 6 22.282444627124235 34.98975909489906 19.7034085261921 23.024387751784612 7 82.26674655037085 6.640332910692805 6.088113583480793 5.004806955455546 8 84.02302694217256 5.129741353285682 4.832035223140266 6.015196481401495 9 77.21201227979732 8.400328823619569 8.957889212448853 5.429769684134261 10 35.12235675504963 34.14772911993238 13.726063916252155 17.003850208765844 11 25.43018768026083 28.298105548666356 27.195060214664235 19.076646556408576 12 26.118023528537528 23.53411080612875 30.88643045241114 19.46143521292258 13 25.286443456735082 24.730740828468324 28.15111024415618 21.831705470640415 14 19.75356806138041 26.838137966588178 30.248986359393072 23.159307612638344 15 18.016329715344636 28.925564178660835 29.661934021633623 23.39617208436091 16 21.5983242999726 27.540371459224477 27.430763586035212 23.43054065476771 17 21.14456628257467 27.54966026203713 27.92887563686354 23.376897818524657 18 22.51396803722952 26.688123801163886 26.415497438612622 24.382410722993967 19 22.299396692257318 30.573165577554533 26.42687622205812 20.700561508130026 20 24.25445746424972 28.314593173658814 25.736021512867314 21.69492784922415 21 23.451208241025856 29.075578344085123 25.734163752304784 21.739049662584236 22 22.26711810248336 26.871577656713715 26.024903280340713 24.83640096046221 23 22.54810438756601 28.10791731107736 24.699158898905317 24.644819402451315 24 20.484132402595293 28.54518770348284 26.448704908667846 24.521974985254026 25 23.22688365310037 28.40655232150404 24.400756108548947 23.965807916846636 26 23.859218904571485 27.75122728307162 24.127897525927374 24.261656286429524 27 20.4885445839313 27.403826057878533 25.63059360094374 26.477035757246426 28 24.636691699990244 26.793319493017144 26.615206699084588 21.954782107908024 29 24.92905676851839 28.69613074918839 23.198785024592105 23.176027457701114 30 24.153673953732476 28.10536289030388 25.345659574665717 22.395303581297924 31 27.172767087913876 28.16248902760168 21.479659844041002 23.185084040443446 32 24.589551025716048 29.2325591116189 22.26595700213178 23.91193286053327 33 24.920000185776058 26.524176431520623 24.637388360201196 23.918435022502123 34 23.600757966309512 27.046207149591524 24.392628406087884 24.960406478011084 35 24.83941982137632 27.922605694965004 25.367488261275444 21.870486222383228 36 24.717736504530613 28.776478893517808 24.98966620687093 21.51611839508065 37 22.831877313492452 30.855777403129398 22.403431283758994 23.90891399961916 38 24.29463153641443 28.469483960559742 25.83401838254077 21.40186612048506 39 22.47170398443197 27.422171443433513 25.698634081546402 24.40749049058812 40 23.561048334285438 28.119528314593172 25.03378802023101 23.28563533089038 41 21.512635094025907 25.43877982286253 27.33485669699461 25.71372838611696 42 24.870769530869012 27.363187545573187 24.19152582519402 23.574517098363778 43 22.67327100546646 25.409984534143316 26.438255005503613 25.478489454886606 44 23.659277424029206 24.519652784550864 25.452016366870556 26.369053424549378 45 25.643830144951767 24.72656086720263 26.900140725362608 22.729468262482992 46 22.154491368379986 25.973118204660196 28.51476687427141 23.357623552688413 47 22.370456033774087 26.008183435277942 26.424321801284638 25.19703872966333 48 22.41713226790765 24.617881874294632 29.370730053457063 23.59425580434066 49 22.80284980470292 25.517270206629416 27.27285393822017 24.407026050447488 50 22.077858745175625 23.825082554234996 26.27430763586035 27.822751064729022 51 22.116407276848125 23.129119003497234 29.443879375606674 25.310594344047963 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1590.0 1 1316.5 2 1043.0 3 736.5 4 430.0 5 430.5 6 431.0 7 460.0 8 489.0 9 596.5 10 704.0 11 805.5 12 907.0 13 921.0 14 935.0 15 985.5 16 1036.0 17 1088.0 18 1140.0 19 1172.5 20 1205.0 21 1261.0 22 1317.0 23 1556.0 24 1795.0 25 2275.5 26 3077.5 27 3399.0 28 4446.5 29 5494.0 30 5876.0 31 6258.0 32 7016.5 33 7775.0 34 9386.5 35 10998.0 36 12103.0 37 13208.0 38 14078.5 39 14949.0 40 15571.5 41 16194.0 42 17988.5 43 19783.0 44 24471.0 45 29159.0 46 32310.5 47 35462.0 48 39016.0 49 42570.0 50 41376.5 51 40183.0 52 35652.0 53 31121.0 54 27565.0 55 24009.0 56 22712.0 57 21415.0 58 20803.5 59 20192.0 60 19212.5 61 18233.0 62 15366.5 63 12500.0 64 11520.0 65 10540.0 66 9289.5 67 8039.0 68 7744.0 69 7449.0 70 6109.0 71 4769.0 72 4165.0 73 3561.0 74 3291.5 75 2482.5 76 1943.0 77 1572.5 78 1202.0 79 912.5 80 623.0 81 559.0 82 495.0 83 320.0 84 145.0 85 118.0 86 91.0 87 69.5 88 48.0 89 28.5 90 9.0 91 7.0 92 5.0 93 3.0 94 1.0 95 1.0 96 1.0 97 2.0 98 3.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 430626.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.232114335082823 #Duplication Level Percentage of deduplicated Percentage of total 1 76.42255892694915 23.868380981860803 2 9.85404104183396 6.155250729623138 3 3.4784209607823366 3.2591532345810768 4 1.7122799217636289 2.1391248916075334 5 1.0482418001951388 1.636940387725381 6 0.6988193549132462 1.3095363595331595 7 0.5286182430336654 1.155690578422663 8 0.39320156530590594 0.9824412995494074 9 0.33786132560600945 0.949691119565658 >10 4.55201985389846 33.9158583147775 >50 0.8887268609357584 18.190404441630278 >100 0.07997794291011451 4.1404116114623095 >500 0.0014949148207498038 0.29951218411739644 >1k 0.0037372870518745096 1.9976038655436952 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCTGCT 3090 0.7175600172771732 No Hit AATGATACGGCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCC 1429 0.33184248048190307 No Hit AATCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCT 1380 0.3204636970364075 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1330 0.3088526935205956 No Hit AATGATACCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGT 1328 0.3083882533799631 No Hit CCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCTGC 681 0.1581418678853576 No Hit AAACTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCT 602 0.13979648233037487 No Hit AAAAACTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTT 451 0.10473125171262301 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.3222007031623729E-4 0.0 0.0 0.8048747637160785 0.0 2 2.3222007031623729E-4 0.0 0.0 1.155759289963913 0.0 3 2.3222007031623729E-4 0.0 0.0 1.5623766330876445 0.0 4 2.3222007031623729E-4 0.0 0.0 3.4473069438445427 0.0 5 2.3222007031623729E-4 0.0 0.0 3.7417619930055315 0.0 6 2.3222007031623729E-4 0.0 0.0 5.144138997645289 0.0 7 2.3222007031623729E-4 0.0 0.0 6.2253556450376895 0.0 8 2.3222007031623729E-4 0.0 0.0 7.183495655162484 0.0 9 2.3222007031623729E-4 0.0 0.0 8.622795650982523 0.0 10 2.3222007031623729E-4 0.0 0.0 10.552776655380772 0.0 11 2.3222007031623729E-4 0.0 0.0 12.329956853510936 0.0 12 2.3222007031623729E-4 0.0 0.0 13.15666030383674 0.0 13 2.3222007031623729E-4 0.0 0.0 13.531463497327147 0.0 14 2.3222007031623729E-4 0.0 0.0 13.962231727763767 0.0 15 2.3222007031623729E-4 0.0 0.0 14.335409380761961 0.0 16 2.3222007031623729E-4 0.0 0.0 14.986786677999007 0.0 17 2.3222007031623729E-4 0.0 0.0 15.819527850153033 0.0 18 2.3222007031623729E-4 0.0 0.0 16.941847449991407 0.0 19 2.3222007031623729E-4 0.0 0.0 17.397463227951864 0.0 20 2.3222007031623729E-4 0.0 0.0 18.000538750563134 0.0 21 2.3222007031623729E-4 0.0 0.0 18.523962789055933 0.0 22 2.3222007031623729E-4 0.0 0.0 19.119839489487397 0.0 23 2.3222007031623729E-4 0.0 0.0 19.716180630059494 0.0 24 2.3222007031623729E-4 0.0 0.0 20.133480096417774 0.0 25 2.3222007031623729E-4 0.0 0.0 20.48250686210308 0.0 26 2.3222007031623729E-4 0.0 0.0 20.825728126030477 0.0 27 2.3222007031623729E-4 0.0 0.0 21.261372977943736 0.0 28 2.3222007031623729E-4 0.0 0.0 21.62177852707454 0.0 29 2.3222007031623729E-4 0.0 0.0 22.01283712548708 0.0 30 2.3222007031623729E-4 0.0 0.0 22.55181990869107 0.0 31 2.3222007031623729E-4 0.0 0.0 22.915476538806296 0.0 32 2.3222007031623729E-4 0.0 0.0 23.293066373140498 0.0 33 4.6444014063247457E-4 0.0 0.0 23.66392182543553 0.0 34 4.6444014063247457E-4 0.0 0.0 24.03222285695708 0.0 35 4.6444014063247457E-4 0.0 0.0 24.411670451853812 0.0 36 4.6444014063247457E-4 0.0 0.0 24.745370692898245 0.0 37 4.6444014063247457E-4 0.0 0.0 25.10925954308379 0.0 38 4.6444014063247457E-4 0.0 0.0 25.46850399186301 0.0 39 4.6444014063247457E-4 0.0 0.0 25.823104039235904 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCCGT 25 3.8867278E-5 45.000004 36 ATCTACG 30 2.1623273E-6 45.000004 1 TTTACCG 30 2.1623273E-6 45.000004 1 GAGTCGA 25 3.8867278E-5 45.000004 20 GGCCGAT 30 2.1623273E-6 45.000004 8 TTCACGG 30 2.1623273E-6 45.000004 2 ATCGTTA 30 2.1623273E-6 45.000004 12 ATCGTAG 25 3.8867278E-5 45.000004 1 GTTACGA 30 2.1623273E-6 45.000004 15 CTAAACG 25 3.8867278E-5 45.000004 1 TACGAGG 30 2.1623273E-6 45.000004 2 TGCGACC 25 3.8867278E-5 45.000004 13 TCTCGGT 25 3.8867278E-5 45.000004 11 GTATCTA 25 3.8867278E-5 45.000004 9 ATGACCG 25 3.8867278E-5 45.000004 1 CTACCCG 25 3.8867278E-5 45.000004 35 AACGTAG 20 7.028367E-4 45.0 1 AATCCTC 20 7.028367E-4 45.0 20 CGTATCG 20 7.028367E-4 45.0 24 GATCGCC 20 7.028367E-4 45.0 9 >>END_MODULE