FastQCFastQC Report
Sat 18 Jun 2016
SRR3553243_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553243_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1190732
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT55150.4631604760769006TruSeq Adapter, Index 16 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12760.10716097325006801No Hit
CCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC11930.10019047107157615TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTT207.033388E-445.0000049
ATTACGT207.033388E-445.00000413
ATAGCCG207.033388E-445.0000041
AGATTCG351.2119744E-745.00000444
ACGTAAG351.2119744E-745.0000041
GACGATA351.2119744E-745.0000049
ATCGGAC351.2119744E-745.00000416
ATTGCGT207.033388E-445.00000444
GCCGTAA207.033388E-445.00000431
CCGATAC253.8908915E-545.025
ATCGAGT253.8908915E-545.021
CGGTATG302.1655687E-644.9999961
TGTAACG302.1655687E-644.9999961
ATACCCG551.8189894E-1244.9999961
TATCGCG850.042.352941
TCGTATA556.184564E-1140.9090929
CATACCG501.0822987E-940.51
TAGTAGG1450.040.344832
TATAGCG950.040.2631571
CGAAACG451.9284926E-840.01
Produced by FastQC (version 0.11.5)