##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553243_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1190732
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.797879791590383	33.0	31.0	34.0	30.0	34.0
2	32.00662953544543	33.0	31.0	34.0	30.0	34.0
3	32.155268355935675	34.0	31.0	34.0	30.0	34.0
4	35.82801839540719	37.0	35.0	37.0	35.0	37.0
5	35.610306937245326	37.0	35.0	37.0	33.0	37.0
6	35.62985709630715	37.0	35.0	37.0	33.0	37.0
7	35.767841126298784	37.0	35.0	37.0	35.0	37.0
8	35.80401803260516	37.0	35.0	37.0	35.0	37.0
9	37.51404766143851	39.0	37.0	39.0	35.0	39.0
10	37.01154835848873	39.0	37.0	39.0	33.0	39.0
11	36.988783370229406	39.0	37.0	39.0	33.0	39.0
12	36.887449064944924	39.0	37.0	39.0	33.0	39.0
13	36.84602580597481	39.0	37.0	39.0	33.0	39.0
14	38.02014307165676	40.0	37.0	41.0	33.0	41.0
15	38.04195402491913	40.0	37.0	41.0	33.0	41.0
16	37.95843649116678	40.0	37.0	41.0	33.0	41.0
17	37.917030028587455	40.0	37.0	41.0	33.0	41.0
18	37.89455981698653	40.0	37.0	41.0	33.0	41.0
19	37.960118649704555	40.0	37.0	41.0	33.0	41.0
20	37.844041312402794	40.0	37.0	41.0	33.0	41.0
21	37.82310881037883	40.0	37.0	41.0	33.0	41.0
22	37.81487605943235	40.0	37.0	41.0	33.0	41.0
23	37.68251210179957	39.0	36.0	41.0	33.0	41.0
24	37.558094516650264	39.0	36.0	41.0	32.0	41.0
25	37.59908694819657	39.0	36.0	41.0	32.0	41.0
26	37.52517107124021	39.0	36.0	41.0	32.0	41.0
27	37.488134189725315	39.0	36.0	41.0	32.0	41.0
28	37.4399184703191	39.0	36.0	41.0	32.0	41.0
29	37.3008636704145	39.0	36.0	41.0	32.0	41.0
30	37.321642485462725	39.0	36.0	41.0	32.0	41.0
31	37.27385927311939	39.0	36.0	41.0	32.0	41.0
32	37.23856417732958	39.0	36.0	41.0	32.0	41.0
33	37.16714088476668	39.0	35.0	41.0	32.0	41.0
34	37.11304894804204	39.0	35.0	41.0	31.0	41.0
35	37.06329551905886	39.0	35.0	41.0	31.0	41.0
36	36.98265688668819	39.0	35.0	41.0	31.0	41.0
37	36.90291602140532	39.0	35.0	41.0	31.0	41.0
38	36.82576012066527	39.0	35.0	41.0	31.0	41.0
39	36.775467527537685	39.0	35.0	40.0	31.0	41.0
40	36.66291575266307	39.0	35.0	40.0	31.0	41.0
41	36.595697436534834	39.0	35.0	40.0	31.0	41.0
42	36.536756381788685	39.0	35.0	40.0	31.0	41.0
43	36.440953967811396	39.0	35.0	40.0	30.0	41.0
44	36.34766681335515	38.0	35.0	40.0	30.0	41.0
45	36.14915950860479	38.0	35.0	40.0	30.0	41.0
46	36.004275521275986	38.0	35.0	40.0	30.0	41.0
47	35.86727575978474	38.0	35.0	40.0	30.0	41.0
48	35.79679726420387	38.0	35.0	40.0	29.0	41.0
49	35.64991618600995	38.0	34.0	40.0	29.0	41.0
50	35.589717081593506	38.0	34.0	40.0	29.0	41.0
51	34.32860290980674	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	5.0
11	10.0
12	14.0
13	19.0
14	34.0
15	46.0
16	98.0
17	173.0
18	331.0
19	620.0
20	1049.0
21	1635.0
22	2330.0
23	3452.0
24	4525.0
25	5766.0
26	7282.0
27	9375.0
28	12269.0
29	15840.0
30	20595.0
31	26901.0
32	35747.0
33	49435.0
34	78197.0
35	92664.0
36	113241.0
37	163903.0
38	252647.0
39	292452.0
40	75.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.02505013722651	30.537182170295246	20.67207398474216	27.765693707736084
2	24.63392266269824	28.9767134838066	20.231924564049677	26.157439289445485
3	21.519703846037565	29.569458114840288	20.71171346701021	28.199124572111945
4	20.64629152487713	31.445950894071885	18.958506196188562	28.949251384862425
5	18.77080652909303	33.32966612134385	18.023199174961285	29.87632817460184
6	20.61706580489984	35.83274825905409	19.455931309480217	24.094254626565846
7	85.29475986200086	4.53771293624426	5.670629495134086	4.496897706620802
8	86.65425973266865	4.053976881447714	4.6035547881471235	4.688208597736518
9	79.60212709492984	7.063218255661223	8.251982813932942	5.082671835475993
10	37.527336126013246	31.885848368902494	13.523278117998005	17.063537387086264
11	28.684708229895563	27.82515293113816	23.788224386343863	19.70191445262242
12	26.968033109045532	24.042101833158092	28.518255997151332	20.47160906064505
13	25.5929125949416	23.942163307948388	27.517527033790977	22.947397063319034
14	21.171262719066927	25.859135388987614	29.303655230564058	23.6659466613814
15	20.80031442843562	27.57858191431825	28.02335034247841	23.597753314767722
16	23.30180090902067	26.184229532758003	27.326804016352966	23.187165541868364
17	22.084650450311237	26.994823352358043	28.15923314398202	22.761293053348698
18	23.96500639942489	25.904065734355004	26.088658069154103	24.042269797066005
19	23.197747268067037	28.894495150881976	25.963524957757077	21.944232623293907
20	26.0012328550841	27.041937228528333	25.389508302455972	21.567321613931597
21	24.504506471649375	27.339401309446625	25.431751225296708	22.724340993607296
22	23.854486148016516	25.039807446175967	25.62373397204409	25.481972433763435
23	24.011952311687264	27.432789242247622	24.215860495896642	24.33939795016847
24	21.972114632007873	26.912185109663632	26.522424861345794	24.593275396982698
25	23.16465837820769	26.65142114262487	24.436229143081732	25.747691336085705
26	24.61754618167648	26.64369480286076	25.59240870321785	23.146350312244905
27	23.06715532966276	24.898885727434887	25.924053439396943	26.10990550350541
28	21.895019198274674	27.81885428459133	26.15794318116923	24.128183335964767
29	23.03280671049405	27.272719638004183	24.925591988793446	24.768881662708317
30	24.334023105115175	24.696321254488833	25.484995784105912	25.484659856290083
31	24.571524070907643	26.42139457073464	24.153965795829794	24.853115562527925
32	24.50677398440623	23.921503747274787	26.086642502259117	25.48507976605987
33	23.00903981752401	24.978248673924945	25.30913757251842	26.703573936032626
34	21.51953588212965	25.1949221151359	26.969376820308856	26.3161651824256
35	22.051225632636058	24.25424024885533	27.839009953541183	25.85552416496743
36	23.190104910256885	27.45554835177017	26.059180403314937	23.295166334658006
37	22.719218094415872	27.056298142655105	25.189379306174693	25.035104456754333
38	22.823691645139295	26.799313363544442	26.481777595630252	23.895217395686014
39	22.494146457809148	23.497982753465934	26.698954928564948	27.30891586015997
40	23.151473211436326	23.60640345602537	27.449249705223345	25.79287362731496
41	20.262074085520503	23.462122459125982	30.108706241202892	26.167097214150626
42	22.94378583929885	24.585213129402753	26.822240436974905	25.648760594323495
43	21.927604196410275	24.34334510200448	27.274567240991253	26.454483460593988
44	24.369967381409083	22.54445164822983	26.45843061243	26.62715035793109
45	24.986478905412802	23.44809747281504	26.87724861681722	24.688175004954935
46	21.268429839796024	23.88463566948734	28.97402606127995	25.87290842943668
47	22.217425919518412	24.38516811507543	27.414229230422965	25.98317673498319
48	22.279236637631307	23.627650890376675	29.42206978564446	24.67104268634756
49	22.335840474598818	23.59943295384688	27.647111188747765	26.41761538280654
50	21.371727643164036	23.127118444788582	27.646691278977976	27.854462633069403
51	22.322655307827453	21.88804869609618	29.378819079356226	26.410476916720132
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1755.0
1	1436.0
2	1117.0
3	842.0
4	567.0
5	547.0
6	527.0
7	568.0
8	609.0
9	699.0
10	789.0
11	840.0
12	891.0
13	1003.5
14	1116.0
15	1159.0
16	1202.0
17	1362.0
18	1522.0
19	1929.0
20	2336.0
21	2451.5
22	2567.0
23	3465.5
24	4364.0
25	5204.0
26	6943.5
27	7843.0
28	10606.0
29	13369.0
30	15530.0
31	17691.0
32	21279.0
33	24867.0
34	26556.5
35	28246.0
36	30679.0
37	33112.0
38	35304.5
39	37497.0
40	40973.5
41	44450.0
42	48329.5
43	52209.0
44	60674.5
45	69140.0
46	80278.5
47	91417.0
48	102130.0
49	112843.0
50	109830.0
51	106817.0
52	99316.5
53	91816.0
54	84784.0
55	77752.0
56	72505.0
57	67258.0
58	63783.0
59	60308.0
60	57850.5
61	55393.0
62	49563.5
63	43734.0
64	40929.5
65	38125.0
66	32205.0
67	26285.0
68	22858.5
69	19432.0
70	17117.0
71	14802.0
72	13032.5
73	11263.0
74	9263.0
75	6351.5
76	5440.0
77	4481.0
78	3522.0
79	2524.5
80	1527.0
81	1275.0
82	1023.0
83	762.5
84	502.0
85	380.5
86	259.0
87	155.5
88	52.0
89	44.5
90	37.0
91	24.5
92	12.0
93	10.5
94	9.0
95	5.5
96	2.0
97	2.5
98	3.0
99	4.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1190732.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.321089830800815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.9949092675721	17.99636835318577
2	10.753924314836121	5.230943185895249
3	4.101293829926441	2.992438069804503
4	1.9823970964063786	1.9285623144807296
5	1.152768343774999	1.4018291221527615
6	0.726445586862834	1.0600769025167882
7	0.531173479507834	0.9043102537594346
8	0.4119340057070098	0.801494716572944
9	0.3179793328637089	0.6960243527024741
>10	3.9673710660589787	23.79530758839462
>50	1.6816216551936574	29.185735566912648
>100	0.37052087537066614	12.121699573747593
>500	0.005919976392199941	1.0298824564658382
>1k	0.0013929356216941038	0.38823858391771404
>5k	3.4823390542352596E-4	0.46708895949087975
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT	5515	0.4631604760769006	TruSeq Adapter, Index 16 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1276	0.10716097325006801	No Hit
CCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	1193	0.10019047107157615	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.398195395773356E-5	0.0	0.0	0.5246352663739615	0.0
2	8.398195395773356E-5	0.0	0.0	0.7678470050355579	0.0
3	8.398195395773356E-5	0.0	0.0	1.0931091127138601	0.0
4	8.398195395773356E-5	0.0	0.0	2.349815071737385	0.0
5	8.398195395773356E-5	0.0	0.0	2.567580278349788	0.0
6	8.398195395773356E-5	0.0	0.0	3.7120023649318235	0.0
7	8.398195395773356E-5	0.0	0.0	4.596668267922589	0.0
8	8.398195395773356E-5	0.0	0.0	5.385090851677791	0.0
9	8.398195395773356E-5	0.0	0.0	6.35340278081046	0.0
10	8.398195395773356E-5	0.0	0.0	7.911352008680375	0.0
11	8.398195395773356E-5	0.0	0.0	9.152437324267762	0.0
12	8.398195395773356E-5	0.0	0.0	9.796830856985451	0.0
13	8.398195395773356E-5	0.0	0.0	10.06515319988041	0.0
14	8.398195395773356E-5	0.0	0.0	10.374038826536953	0.0
15	8.398195395773356E-5	0.0	0.0	10.653446787354333	0.0
16	8.398195395773356E-5	0.0	0.0	11.162713356154029	0.0
17	8.398195395773356E-5	0.0	0.0	11.785019634980836	0.0
18	8.398195395773356E-5	0.0	0.0	12.513731049472089	0.0
19	8.398195395773356E-5	0.0	0.0	12.888206582169623	0.0
20	8.398195395773356E-5	0.0	0.0	13.290480141627167	0.0
21	8.398195395773356E-5	0.0	0.0	13.7539765455199	0.0
22	8.398195395773356E-5	0.0	0.0	14.239812149165388	0.0
23	8.398195395773356E-5	0.0	0.0	14.714310189026582	0.0
24	8.398195395773356E-5	0.0	0.0	15.123638232616575	0.0
25	8.398195395773356E-5	0.0	0.0	15.47283519717283	0.0
26	8.398195395773356E-5	0.0	0.0	15.781468877967502	0.0
27	8.398195395773356E-5	0.0	0.0	16.10723487736955	0.0
28	8.398195395773356E-5	0.0	0.0	16.436948028607613	0.0
29	8.398195395773356E-5	0.0	0.0	16.79387133292798	0.0
30	1.6796390791546712E-4	0.0	0.0	17.27517191105975	0.0
31	1.6796390791546712E-4	0.0	0.0	17.625796568833287	0.0
32	1.6796390791546712E-4	0.0	0.0	17.997920606820006	0.0
33	1.6796390791546712E-4	0.0	0.0	18.353668163784967	0.0
34	1.6796390791546712E-4	0.0	0.0	18.687664394674872	0.0
35	1.6796390791546712E-4	0.0	0.0	19.04232018623838	0.0
36	1.6796390791546712E-4	0.0	0.0	19.395632266538566	0.0
37	1.6796390791546712E-4	0.0	0.0	19.73080424478388	0.0
38	1.6796390791546712E-4	0.0	0.0	20.0888193145057	0.0
39	1.6796390791546712E-4	0.0	0.0	20.457668056288064	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTT	20	7.033388E-4	45.000004	9
ATTACGT	20	7.033388E-4	45.000004	13
ATAGCCG	20	7.033388E-4	45.000004	1
AGATTCG	35	1.2119744E-7	45.000004	44
ACGTAAG	35	1.2119744E-7	45.000004	1
GACGATA	35	1.2119744E-7	45.000004	9
ATCGGAC	35	1.2119744E-7	45.000004	16
ATTGCGT	20	7.033388E-4	45.000004	44
GCCGTAA	20	7.033388E-4	45.000004	31
CCGATAC	25	3.8908915E-5	45.0	25
ATCGAGT	25	3.8908915E-5	45.0	21
CGGTATG	30	2.1655687E-6	44.999996	1
TGTAACG	30	2.1655687E-6	44.999996	1
ATACCCG	55	1.8189894E-12	44.999996	1
TATCGCG	85	0.0	42.35294	1
TCGTATA	55	6.184564E-11	40.90909	29
CATACCG	50	1.0822987E-9	40.5	1
TAGTAGG	145	0.0	40.34483	2
TATAGCG	95	0.0	40.263157	1
CGAAACG	45	1.9284926E-8	40.0	1
>>END_MODULE