SRR3553242_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553242_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	514648
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGCT	2641	0.5131662806423031	Illumina Single End Adapter 1 (96% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1256	0.24405030234257202	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCC	987	0.19178156720710077	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGT	930	0.18070603597021653	No Hit
AATCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCT	888	0.1725451182167229	Illumina Single End Adapter 1 (95% over 22bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	827	0.16069235671760115	No Hit
AAACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCT	644	0.12513407222023595	Illumina Single End Adapter 1 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAA	25	3.8877915E-5	45.000004	33
CCCTAGA	25	3.8877915E-5	45.000004	33
CATTCGG	25	3.8877915E-5	45.000004	2
CGTTCGA	25	3.8877915E-5	45.000004	31
TGCGCAC	25	3.8877915E-5	45.000004	14
TTGCGCC	25	3.8877915E-5	45.000004	19
AACGACT	25	3.8877915E-5	45.000004	37
ATTATCC	50	2.1827873E-11	45.000004	45
CGACTTT	25	3.8877915E-5	45.000004	18
CCTATCA	25	3.8877915E-5	45.000004	42
TATAACG	50	2.1827873E-11	45.000004	1
CGATAGG	25	3.8877915E-5	45.000004	2
TACACCG	25	3.8877915E-5	45.000004	1
ACGACTA	25	3.8877915E-5	45.000004	38
ATCGAAC	25	3.8877915E-5	45.000004	10
AGTCGCA	25	3.8877915E-5	45.000004	31
GTCATGC	20	7.029651E-4	45.0	2
ACGCCTA	20	7.029651E-4	45.0	30
AATTTCG	20	7.029651E-4	45.0	37
CCGATTG	20	7.029651E-4	45.0	12