##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553242_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	514648
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80770546081982	33.0	31.0	34.0	30.0	34.0
2	32.00626641898929	33.0	31.0	34.0	30.0	34.0
3	32.191754364147926	34.0	31.0	34.0	30.0	34.0
4	35.83797469338266	37.0	35.0	37.0	35.0	37.0
5	35.61023845424445	37.0	35.0	37.0	33.0	37.0
6	34.913018218277344	37.0	35.0	37.0	33.0	37.0
7	35.32947762354075	37.0	35.0	37.0	33.0	37.0
8	35.729277098133096	37.0	35.0	37.0	35.0	37.0
9	37.388391677418355	39.0	37.0	39.0	35.0	39.0
10	36.97893705989336	39.0	37.0	39.0	33.0	39.0
11	36.97746420854642	39.0	37.0	39.0	33.0	39.0
12	36.858244858621816	39.0	37.0	39.0	33.0	39.0
13	36.79601203152446	39.0	37.0	39.0	33.0	39.0
14	37.98571645085573	40.0	37.0	41.0	33.0	41.0
15	38.02051499277176	40.0	37.0	41.0	33.0	41.0
16	37.926825713886	40.0	37.0	41.0	33.0	41.0
17	37.92841320669662	40.0	37.0	41.0	33.0	41.0
18	37.891415103138456	40.0	37.0	41.0	33.0	41.0
19	37.88914170462141	40.0	37.0	41.0	33.0	41.0
20	37.78994769240335	40.0	37.0	41.0	33.0	41.0
21	37.74411830999052	40.0	36.0	41.0	33.0	41.0
22	37.73353826304581	40.0	36.0	41.0	33.0	41.0
23	37.61565963532356	39.0	36.0	41.0	33.0	41.0
24	37.45635852077537	39.0	36.0	41.0	32.0	41.0
25	37.48240933608991	39.0	36.0	41.0	32.0	41.0
26	37.45132206867606	39.0	36.0	41.0	32.0	41.0
27	37.341987144611466	39.0	36.0	41.0	32.0	41.0
28	37.25709611229423	39.0	36.0	41.0	32.0	41.0
29	37.01027304099112	39.0	35.0	41.0	31.0	41.0
30	37.05413020161353	39.0	35.0	41.0	31.0	41.0
31	37.00715634763955	39.0	35.0	41.0	31.0	41.0
32	36.996896908178016	39.0	35.0	41.0	31.0	41.0
33	36.72145427554367	39.0	35.0	41.0	31.0	41.0
34	36.73516073121823	39.0	35.0	41.0	31.0	41.0
35	36.66455713419658	39.0	35.0	41.0	30.0	41.0
36	36.59821858823895	39.0	35.0	40.0	30.0	41.0
37	36.48732920364987	39.0	35.0	40.0	30.0	41.0
38	36.302548538029875	39.0	35.0	40.0	30.0	41.0
39	36.295083241361084	39.0	35.0	40.0	30.0	41.0
40	36.20983856927453	38.0	35.0	40.0	30.0	41.0
41	36.19398112884923	38.0	35.0	40.0	30.0	41.0
42	36.160161897063624	38.0	35.0	40.0	30.0	41.0
43	36.07789984610841	38.0	35.0	40.0	30.0	41.0
44	35.97762548382584	38.0	35.0	40.0	30.0	41.0
45	35.74479450031866	38.0	35.0	40.0	29.0	41.0
46	35.55498321182633	38.0	34.0	40.0	28.0	41.0
47	35.414677604887224	37.0	34.0	40.0	28.0	41.0
48	35.350688237397215	37.0	34.0	40.0	28.0	41.0
49	35.253033141098385	37.0	34.0	40.0	28.0	41.0
50	35.166539071365285	37.0	34.0	40.0	28.0	41.0
51	33.95675879436042	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	2.0
11	3.0
12	2.0
13	9.0
14	21.0
15	29.0
16	65.0
17	142.0
18	240.0
19	460.0
20	808.0
21	1167.0
22	1685.0
23	2109.0
24	2613.0
25	3116.0
26	3713.0
27	4550.0
28	5670.0
29	7226.0
30	9424.0
31	12140.0
32	16034.0
33	21508.0
34	33871.0
35	42771.0
36	50975.0
37	73763.0
38	103528.0
39	116961.0
40	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.860083008192007	31.38028322270756	21.132502215106246	26.62713155399419
2	24.459436352613825	30.230759664858308	20.250928790163375	25.05887519236449
3	21.494885824874476	30.71788873171566	20.05370661112061	27.733518832289256
4	21.304270103060734	31.145559683511838	19.306399713979264	28.243770499448168
5	18.935077956195304	33.967877073261725	17.3759540501469	29.721090920396076
6	20.088876280486858	38.89920877959304	17.995406569150177	23.016508370769927
7	84.08115838398284	5.896457384464721	5.307316845688704	4.715067385863736
8	85.84838569274534	5.051802396978129	4.296528889648847	4.803283020627691
9	79.77957749762945	7.938241283362609	7.4462545273662775	4.835926691641665
10	37.949433399138826	32.563616296964135	12.188524972408326	17.298425331488705
11	28.106589358163248	29.79959117688206	22.307480064043776	19.78633940091091
12	27.18634872767406	24.90964698201489	28.462172203136905	19.441832087174145
13	26.679011673998538	25.748084127403587	25.689208935039094	21.88369526355878
14	22.25890317265393	27.879055198893226	27.65909903468001	22.202942593772832
15	20.37606286238361	28.994769240335145	27.082005564968675	23.54716233231257
16	22.709113802054997	28.63879778023037	25.59147222956273	23.060616188151904
17	22.3049540656915	26.76800453902473	26.623439710248558	24.303601685035208
18	24.790730751892557	25.102788702180906	26.110273429606256	23.99620711632028
19	24.42310083785422	28.804153518521396	25.5399807246895	21.232764918934883
20	25.63169389563352	28.05937261973232	23.868158430616653	22.440775054017504
21	25.31244656541947	27.80929878285741	24.577381044908364	22.300873606814754
22	24.193623602928604	25.968623214313475	24.59817195442322	25.239581228334707
23	23.772947723492564	27.88915919230231	23.462055618597567	24.87583746560756
24	21.42027171969968	28.90655360557119	24.766053691066514	24.90712098366262
25	24.218106356189082	27.757418662853055	23.524622654707684	24.499852326250174
26	25.599827454881783	26.65103138455799	23.86932427601001	23.879816884550216
27	22.969291632338997	26.700579813775626	24.31623167679657	26.013896877088804
28	24.322060903763347	27.404944738928354	25.216458628033138	23.056535729275154
29	23.609729368422688	28.310806609566153	23.719513142963734	24.359950879047425
30	24.232679423606037	27.737210676034884	23.847367521101802	24.18274237925728
31	24.918779437596182	28.851370256952325	21.311459482986432	24.918390822465064
32	25.205966019492937	26.9020767592607	23.092676936469196	24.79928028477717
33	22.822200805210553	27.99175358691766	23.489452985341437	25.69659262253035
34	22.996883306648428	27.90004041597364	24.440199903623448	24.66287637375449
35	23.718735912701497	26.792487292285212	24.19498375588752	25.293793039125774
36	24.20664220982108	30.01371811412849	23.019811599384436	22.759828076665993
37	23.13270429497443	31.192776421942764	21.709789992383143	23.964729290699662
38	24.006505417294928	29.373863300741476	23.592241687522346	23.02738959444125
39	23.82288476784132	27.12591907478509	23.661415180861482	25.3897809765121
40	24.639753773452924	26.96736410128865	24.382296249086753	24.010585876171675
41	21.847748363930297	26.09103698061588	25.764017347779454	26.29719730767437
42	24.430873140476596	25.476442150751584	23.76711849652578	26.325566212246038
43	22.356251262999177	25.217818780992058	25.961433834387776	26.46449612162099
44	24.96580186846155	24.01563787287622	24.398618084593743	26.61994217406849
45	25.069756416035816	24.30962521956755	25.435248946852994	25.18536941754364
46	22.77887021809081	25.290295502945703	27.69388008891514	24.236954190048344
47	22.759050846403756	27.348595544916137	25.457594316892322	24.434759291787785
48	22.532293917395968	25.661422953164102	27.1426295254232	24.663653604016726
49	23.227526386967405	25.633636971289114	26.164873855528437	24.973962786215047
50	22.430476753042857	25.16302404750431	26.078406988854518	26.328092210598314
51	22.974926551740218	24.04614416066904	27.344709393604948	25.634219893985794
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1576.0
1	1261.0
2	946.0
3	674.5
4	403.0
5	377.5
6	352.0
7	383.0
8	414.0
9	455.0
10	496.0
11	518.0
12	540.0
13	601.5
14	663.0
15	702.5
16	742.0
17	794.0
18	846.0
19	867.5
20	889.0
21	999.5
22	1110.0
23	1983.0
24	2856.0
25	2836.0
26	3053.5
27	3291.0
28	4359.0
29	5427.0
30	6136.5
31	6846.0
32	7773.0
33	8700.0
34	10668.0
35	12636.0
36	12789.5
37	12943.0
38	14557.0
39	16171.0
40	17179.5
41	18188.0
42	20266.5
43	22345.0
44	27443.0
45	32541.0
46	35150.5
47	37760.0
48	42642.0
49	47524.0
50	46916.0
51	46308.0
52	41673.5
53	37039.0
54	34905.5
55	32772.0
56	32252.0
57	31732.0
58	29227.5
59	26723.0
60	24645.5
61	22568.0
62	21254.5
63	19941.0
64	17756.0
65	15571.0
66	14024.5
67	12478.0
68	11137.0
69	9796.0
70	8134.5
71	6473.0
72	5653.0
73	4833.0
74	4458.5
75	3198.0
76	2312.0
77	1819.0
78	1326.0
79	1042.5
80	759.0
81	640.0
82	521.0
83	380.0
84	239.0
85	163.0
86	87.0
87	55.5
88	24.0
89	19.0
90	14.0
91	11.5
92	9.0
93	10.0
94	11.0
95	8.5
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	514648.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.997933368496195
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.73082149897293	19.948427846402737
2	9.781077006548538	5.0857557657675825
3	3.3959259551013434	2.648611701152146
4	1.6561007450141791	1.7222078728158212
5	0.9545727592458358	1.2408459495127402
6	0.6085086540694944	0.9491980465591209
7	0.4580046521661718	0.8335022100634187
8	0.31156915614311353	0.6480123328869802
9	0.24290515573264587	0.5683528848241367
>10	3.939195788606383	26.01652994955441
>50	1.6761562592475057	30.575396956086625
>100	0.23988194889320735	8.160316263278927
>500	0.0037717287561825057	0.8385847714919656
>1k	0.0015086915024730022	0.7642574496034121
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGCT	2641	0.5131662806423031	Illumina Single End Adapter 1 (96% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1256	0.24405030234257202	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCC	987	0.19178156720710077	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGT	930	0.18070603597021653	No Hit
AATCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCT	888	0.1725451182167229	Illumina Single End Adapter 1 (95% over 22bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	827	0.16069235671760115	No Hit
AAACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCT	644	0.12513407222023595	Illumina Single End Adapter 1 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5877803858171021	0.0
2	0.0	0.0	0.0	0.8149259299560088	0.0
3	0.0	0.0	0.0	1.0879280595669274	0.0
4	0.0	0.0	0.0	2.3134258755498904	0.0
5	0.0	0.0	0.0	2.491994528298954	0.0
6	0.0	0.0	0.0	3.483546035348432	0.0
7	0.0	0.0	0.0	4.181304503272139	0.0
8	0.0	0.0	0.0	4.779966112760566	0.0
9	0.0	0.0	0.0	5.679804448866021	0.0
10	0.0	0.0	0.0	6.9634002269512365	0.0
11	0.0	0.0	0.0	8.136823615364287	0.0
12	0.0	0.0	0.0	8.686908333462872	0.0
13	0.0	0.0	0.0	8.926878176928698	0.0
14	0.0	0.0	0.0	9.204155072981921	0.0
15	0.0	0.0	0.0	9.45889229143026	0.0
16	0.0	0.0	0.0	9.954570891172219	0.0
17	0.0	0.0	0.0	10.529332670096842	0.0
18	0.0	0.0	0.0	11.433834387775722	0.0
19	0.0	0.0	0.0	11.746086645629635	0.0
20	0.0	0.0	0.0	12.222334488815656	0.0
21	0.0	0.0	0.0	12.594239169296296	0.0
22	0.0	0.0	0.0	13.003645209929894	0.0
23	0.0	0.0	0.0	13.429373086070479	0.0
24	0.0	0.0	0.0	13.741431036358831	0.0
25	0.0	0.0	0.0	14.009575476830767	0.0
26	0.0	0.0	0.0	14.267033001196934	0.0
27	0.0	0.0	0.0	14.61212323763038	0.0
28	0.0	0.0	0.0	14.89717243630598	0.0
29	0.0	0.0	0.0	15.171923334006934	0.0
30	0.0	0.0	0.0	15.621162425580202	0.0
31	0.0	0.0	0.0	15.889889788748814	0.0
32	0.0	0.0	0.0	16.165029301580887	0.0
33	0.0	0.0	0.0	16.447746809469773	0.0
34	0.0	0.0	0.0	16.725023705522997	0.0
35	0.0	0.0	0.0	17.03008658345121	0.0
36	0.0	0.0	0.0	17.31299839890566	0.0
37	0.0	0.0	0.0	17.572398998927422	0.0
38	0.0	0.0	0.0	17.853173431160716	0.0
39	0.0	0.0	0.0	18.154544465343303	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAA	25	3.8877915E-5	45.000004	33
CCCTAGA	25	3.8877915E-5	45.000004	33
CATTCGG	25	3.8877915E-5	45.000004	2
CGTTCGA	25	3.8877915E-5	45.000004	31
TGCGCAC	25	3.8877915E-5	45.000004	14
TTGCGCC	25	3.8877915E-5	45.000004	19
AACGACT	25	3.8877915E-5	45.000004	37
ATTATCC	50	2.1827873E-11	45.000004	45
CGACTTT	25	3.8877915E-5	45.000004	18
CCTATCA	25	3.8877915E-5	45.000004	42
TATAACG	50	2.1827873E-11	45.000004	1
CGATAGG	25	3.8877915E-5	45.000004	2
TACACCG	25	3.8877915E-5	45.000004	1
ACGACTA	25	3.8877915E-5	45.000004	38
ATCGAAC	25	3.8877915E-5	45.000004	10
AGTCGCA	25	3.8877915E-5	45.000004	31
GTCATGC	20	7.029651E-4	45.0	2
ACGCCTA	20	7.029651E-4	45.0	30
AATTTCG	20	7.029651E-4	45.0	37
CCGATTG	20	7.029651E-4	45.0	12
>>END_MODULE