##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553240_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	545400
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.883465346534653	33.0	31.0	34.0	30.0	34.0
2	32.08454895489549	33.0	31.0	34.0	30.0	34.0
3	32.24737440410708	34.0	31.0	34.0	30.0	34.0
4	35.897513751375136	37.0	35.0	37.0	35.0	37.0
5	35.67771910524386	37.0	35.0	37.0	33.0	37.0
6	35.015150348368174	37.0	35.0	37.0	33.0	37.0
7	35.44663549688302	37.0	35.0	37.0	33.0	37.0
8	35.8397561422809	37.0	35.0	37.0	35.0	37.0
9	37.49745874587459	39.0	37.0	39.0	35.0	39.0
10	37.09663366336633	39.0	37.0	39.0	33.0	39.0
11	37.106820682068204	39.0	37.0	39.0	34.0	39.0
12	37.010555555555555	39.0	37.0	39.0	33.0	39.0
13	36.968542354235424	39.0	37.0	39.0	33.0	39.0
14	38.16374770810415	40.0	37.0	41.0	33.0	41.0
15	38.15341400806747	40.0	37.0	41.0	33.0	41.0
16	38.07065639897323	40.0	37.0	41.0	33.0	41.0
17	38.05208837550422	40.0	37.0	41.0	33.0	41.0
18	38.03921158782545	40.0	37.0	41.0	33.0	41.0
19	38.08256875687569	40.0	37.0	41.0	33.0	41.0
20	37.95062889622296	40.0	37.0	41.0	33.0	41.0
21	37.966683168316834	40.0	37.0	41.0	33.0	41.0
22	37.933892555922256	40.0	37.0	41.0	33.0	41.0
23	37.81354968830216	40.0	37.0	41.0	33.0	41.0
24	37.694319765309864	40.0	36.0	41.0	33.0	41.0
25	37.712988632196556	40.0	36.0	41.0	33.0	41.0
26	37.69243674367437	40.0	36.0	41.0	33.0	41.0
27	37.60079574624129	40.0	36.0	41.0	33.0	41.0
28	37.51399339933993	40.0	36.0	41.0	33.0	41.0
29	37.297411074440774	39.0	36.0	41.0	32.0	41.0
30	37.32468280161349	39.0	36.0	41.0	32.0	41.0
31	37.27899889988999	39.0	36.0	41.0	32.0	41.0
32	37.24478364503117	39.0	36.0	41.0	32.0	41.0
33	37.01655848918225	39.0	35.0	41.0	31.0	41.0
34	36.98677117711771	39.0	35.0	41.0	31.0	41.0
35	36.88870920425376	39.0	35.0	41.0	31.0	41.0
36	36.89396956362303	39.0	35.0	41.0	31.0	41.0
37	36.75504950495049	39.0	35.0	41.0	31.0	41.0
38	36.61565273193986	39.0	35.0	41.0	30.0	41.0
39	36.6274569123579	39.0	35.0	40.0	30.0	41.0
40	36.57750825082508	39.0	35.0	40.0	30.0	41.0
41	36.489365603226986	39.0	35.0	40.0	30.0	41.0
42	36.404792812614595	39.0	35.0	40.0	30.0	41.0
43	36.323006967363405	39.0	35.0	40.0	30.0	41.0
44	36.26117895122846	38.0	35.0	40.0	30.0	41.0
45	36.12	38.0	35.0	40.0	30.0	41.0
46	36.039024569123576	38.0	35.0	40.0	30.0	41.0
47	35.9212834616795	38.0	35.0	40.0	30.0	41.0
48	35.812200220022	38.0	35.0	40.0	29.0	41.0
49	35.6793032636597	38.0	34.0	40.0	29.0	41.0
50	35.61409057572424	38.0	34.0	40.0	28.0	41.0
51	34.40262559589292	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	2.0
11	3.0
12	5.0
13	9.0
14	7.0
15	16.0
16	39.0
17	82.0
18	170.0
19	347.0
20	666.0
21	1029.0
22	1509.0
23	2055.0
24	2409.0
25	2694.0
26	3400.0
27	4140.0
28	5224.0
29	6918.0
30	9066.0
31	11930.0
32	15759.0
33	22054.0
34	34052.0
35	42530.0
36	51032.0
37	75164.0
38	114596.0
39	138456.0
40	35.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.961312797946462	31.07040704070407	21.281444811147782	27.68683535020169
2	22.60964429776311	30.139163916391638	21.83901723505684	25.412174550788414
3	21.496699669966997	30.046387972130546	20.52016868353502	27.936743674367438
4	20.69068573524019	31.601760176017603	18.736707004033736	28.970847084708474
5	18.477081041437476	33.004583791712506	17.72973964063073	30.788595526219286
6	19.764759809314263	37.70682068206821	20.26347634763476	22.264943160982764
7	85.24624129079574	5.18005133846718	5.465896589658966	4.107810781078108
8	86.41235790245692	4.608910891089109	4.549321598826549	4.42940960762743
9	78.82636596993032	8.12871287128713	8.139713971397141	4.905207187385406
10	35.57590759075907	34.55757242390906	13.32874954162083	16.53777044371104
11	26.768793546021268	28.71048771543821	25.420975430876418	19.0997433076641
12	27.103593692702603	22.48349834983498	30.8003300330033	19.612577924459114
13	27.133846718005135	23.653832049871653	27.506050605060505	21.706270627062707
14	22.755225522552255	26.913091309130916	27.99028236156949	22.34140080674734
15	20.565823248991567	28.68976897689769	29.258525852585258	21.485881921525486
16	22.86798679867987	27.11789512284562	27.38980564723139	22.624312431243123
17	21.815914924825815	27.045471213788048	28.41639163916392	22.72222222222222
18	23.501833516685004	26.03043637697103	26.35313531353135	24.114594792812618
19	23.876237623762375	29.071690502383575	25.729189585625228	21.322882288228822
20	24.887972130546387	27.004033736707004	26.136046938027135	21.971947194719473
21	25.63586358635864	26.879721305463878	25.685551888522184	21.7988632196553
22	23.106160616061604	25.85020168683535	26.652915291529155	24.390722405573893
23	23.770810414374772	27.373120645397876	24.733590025669233	24.122478914558123
24	21.542170883755045	27.48184818481848	26.314081408140815	24.661899523285662
25	22.005500550055004	28.16336633663366	24.936560322698938	24.894572790612397
26	24.226072607260726	26.986248624862487	25.378254492115875	23.40942427576091
27	22.00880088008801	26.396222955628897	26.85130179684635	24.743674367436743
28	21.998716538320497	27.434176751008433	28.46021268793546	22.106894022735606
29	24.824165749908325	27.47799779977998	25.279427942794282	22.41840850751742
30	23.992849284928493	25.362669600293366	28.01906857352402	22.625412541254125
31	25.121012101210123	28.165016501650165	23.680968096809682	23.033003300330034
32	25.284745141180785	25.85735240190686	26.422258892555924	22.435643564356436
33	24.7007700770077	26.788412174550793	26.234506784011735	22.276310964429776
34	24.154748808214155	25.545654565456545	27.654015401540157	22.645581224789147
35	25.284011734506784	25.086541987532087	27.502566923359	22.126879354602128
36	25.272827282728272	27.08874220755409	27.325632563256324	20.312797946461313
37	24.863219655298863	27.63201320132013	25.991749174917494	21.51301796846351
38	24.91144114411441	26.5984598459846	26.68848551521819	21.8016134946828
39	25.574990832416578	24.744957829116245	25.94169416941694	23.73835716905024
40	26.426292629262925	24.293912724605793	26.730289695636227	22.54950495049505
41	22.502750275027502	23.933810047671432	29.875137513751376	23.688302163549686
42	24.871653832049873	25.53960396039604	26.802346901356806	22.786395306197285
43	23.51081774844151	24.841950861752842	27.680418041804177	23.966813348001466
44	23.89310597726439	23.48349834983498	27.203703703703702	25.41969196919692
45	24.7011367803447	24.02273560689402	28.21873854052072	23.057389072240557
46	23.768426842684267	24.275210854418773	29.554822148881556	22.4015401540154
47	23.851301796846354	24.504950495049506	28.056839017235056	23.586908690869087
48	23.339933993399338	23.723138980564723	30.38082141547488	22.556105610561055
49	23.178584525119177	24.383388338833882	28.47671433810048	23.96131279794646
50	23.064539787312064	22.90740740740741	27.94316098276494	26.084891822515583
51	23.092042537587094	22.31151448478181	29.885955262192887	24.710487715438212
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1596.0
1	1329.0
2	1062.0
3	741.0
4	420.0
5	407.0
6	394.0
7	410.0
8	426.0
9	471.5
10	517.0
11	599.5
12	682.0
13	815.5
14	949.0
15	923.5
16	898.0
17	989.5
18	1081.0
19	1036.0
20	991.0
21	1158.0
22	1325.0
23	1871.0
24	2417.0
25	2837.5
26	3942.0
27	4626.0
28	5802.0
29	6978.0
30	8410.5
31	9843.0
32	10829.0
33	11815.0
34	13328.0
35	14841.0
36	16734.5
37	18628.0
38	19065.0
39	19502.0
40	20635.5
41	21769.0
42	23455.0
43	25141.0
44	29340.5
45	33540.0
46	37568.0
47	41596.0
48	46034.0
49	50472.0
50	48447.0
51	46422.0
52	42726.0
53	39030.0
54	35868.5
55	32707.0
56	30707.0
57	28707.0
58	27288.0
59	25869.0
60	24950.0
61	24031.0
62	22074.5
63	20118.0
64	17764.0
65	15410.0
66	13666.5
67	11923.0
68	9968.0
69	8013.0
70	7163.0
71	6313.0
72	5621.0
73	4929.0
74	4010.0
75	2551.0
76	2011.0
77	1533.0
78	1055.0
79	800.5
80	546.0
81	373.5
82	201.0
83	157.5
84	114.0
85	95.0
86	76.0
87	63.0
88	50.0
89	29.5
90	9.0
91	6.0
92	3.0
93	2.5
94	2.0
95	2.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	545400.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.63352846764172
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.65157410984432	20.14868352676446
2	10.155547483000287	5.409561259859508
3	3.5667049476364765	2.84981813275664
4	1.6653520639612303	1.7741680641662927
5	0.9902017455819639	1.318628318983288
6	0.6219808495814487	0.9939326798193298
7	0.4681316574851304	0.8727598478364166
8	0.3732823927572894	0.7953461787176548
9	0.3171781006053307	0.7602814774606144
>10	4.538579737925531	30.706347139748114
>50	1.4918507794253129	26.772118553217656
>100	0.154015566153716	5.941715083193257
>500	0.003500353776220818	0.6585539170250914
>1k	0.002100212265732491	0.9980858204516744
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGCT	3062	0.5614228089475615	Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1257	0.2304730473047305	No Hit
CTCCCTGGGAATGATACCTGTCTCTTATACACATCTGACGCTGTAGGGTTC	1034	0.1895856252291896	No Hit
CCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC	815	0.14943160982764941	No Hit
AAACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCT	786	0.14411441144114412	No Hit
CTCCCTGGGAACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGC	727	0.1332966629996333	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	657	0.12046204620462046	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTT	547	0.10029336266960029	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.5005500550055E-4	0.0	0.0	0.6178951228456179	0.0
2	5.5005500550055E-4	0.0	0.0	0.9184085075174184	0.0
3	5.5005500550055E-4	0.0	0.0	1.2332233223322333	0.0
4	5.5005500550055E-4	0.0	0.0	2.6593325999266595	0.0
5	5.5005500550055E-4	0.0	0.0	2.8835716905023836	0.0
6	5.5005500550055E-4	0.0	0.0	4.006050605060506	0.0
7	5.5005500550055E-4	0.0	0.0	4.782361569490282	0.0
8	5.5005500550055E-4	0.0	0.0	5.392189218921892	0.0
9	5.5005500550055E-4	0.0	0.0	6.2455078841217455	0.0
10	5.5005500550055E-4	0.0	0.0	7.618261826182618	0.0
11	5.5005500550055E-4	0.0	0.0	8.765126512651266	0.0
12	5.5005500550055E-4	0.0	0.0	9.52988632196553	0.0
13	5.5005500550055E-4	0.0	0.0	9.803813714704804	0.0
14	5.5005500550055E-4	0.0	0.0	10.106160616061606	0.0
15	5.5005500550055E-4	0.0	0.0	10.356618995232857	0.0
16	5.5005500550055E-4	0.0	0.0	10.896406307297397	0.0
17	5.5005500550055E-4	0.0	0.0	11.546571323799046	0.0
18	5.5005500550055E-4	0.0	0.0	12.546571323799046	0.0
19	5.5005500550055E-4	0.0	0.0	12.90887422075541	0.0
20	5.5005500550055E-4	0.0	0.0	13.413091309130913	0.0
21	5.5005500550055E-4	0.0	0.0	13.855702236890355	0.0
22	5.5005500550055E-4	0.0	0.0	14.377521085441877	0.0
23	5.5005500550055E-4	0.0	0.0	14.866153281994865	0.0
24	5.5005500550055E-4	0.0	0.0	15.241840850751743	0.0
25	5.5005500550055E-4	0.0	0.0	15.556105610561056	0.0
26	5.5005500550055E-4	0.0	0.0	15.84965163182985	0.0
27	5.5005500550055E-4	0.0	0.0	16.255042170883755	0.0
28	5.5005500550055E-4	0.0	0.0	16.604877154382105	0.0
29	5.5005500550055E-4	0.0	0.0	16.92867620095343	0.0
30	5.5005500550055E-4	0.0	0.0	17.46974697469747	0.0
31	5.5005500550055E-4	0.0	0.0	17.786395306197285	0.0
32	5.5005500550055E-4	0.0	0.0	18.117895122845617	0.0
33	5.5005500550055E-4	0.0	0.0	18.434360102676933	0.0
34	5.5005500550055E-4	0.0	0.0	18.73982398239824	0.0
35	5.5005500550055E-4	0.0	0.0	19.07059039237257	0.0
36	5.5005500550055E-4	0.0	0.0	19.392372570590393	0.0
37	5.5005500550055E-4	0.0	0.0	19.728822882288227	0.0
38	5.5005500550055E-4	0.0	0.0	20.06307297396406	0.0
39	5.5005500550055E-4	0.0	0.0	20.414374770810415	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	30	2.163395E-6	45.000004	1
GCGACGA	35	1.2103555E-7	45.000004	33
CTCAACG	55	1.8189894E-12	45.000004	1
ATGTACC	30	2.163395E-6	45.000004	21
GCGATTA	30	2.163395E-6	45.000004	8
ATAACGG	70	0.0	45.000004	2
CGCACAT	35	1.2103555E-7	45.000004	28
GAATTCG	30	2.163395E-6	45.000004	12
TTACCGG	30	2.163395E-6	45.000004	2
CAATACG	35	1.2103555E-7	45.000004	1
ACCCACG	30	2.163395E-6	45.000004	1
TCAACTC	30	2.163395E-6	45.000004	39
CACGCTA	20	7.0300244E-4	45.0	27
AATATCG	20	7.0300244E-4	45.0	1
ATCGTGT	20	7.0300244E-4	45.0	17
CGTTATG	20	7.0300244E-4	45.0	1
GTAATAG	20	7.0300244E-4	45.0	1
TATCTCG	20	7.0300244E-4	45.0	1
TGCGACG	20	7.0300244E-4	45.0	1
CAAGGCG	90	0.0	45.0	1
>>END_MODULE